Q9EQT3 (RHOU_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho-related GTP-binding protein RhoU

Alternative name(s):
Rho GTPase-like protein ARHU
Wnt-1 responsive Cdc42 homolog 1
Short name=WRCH-1

Gene names

Name:	Rhou
Synonyms:	Arhu, G28k, Wrch1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape By similarity. UniProtKB Q7L0Q8
Cofactor	Magnesium By similarity. UniProtKB Q7L0Q8
Subunit structure	Interacts with PAK3 By similarity. Interacts with ARHGAP30 in a GTP-independent manner. In its GTP-loaded conformation, interacts with ARHGAP31. Interacts with PTK2B/PYK2 By similarity. UniProtKB Q7L0Q8
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Cell junction › focal adhesion By similarity. Cell projection › podosome By similarity. Note: Localizes to podosomes in SRC-transformed cells By similarity. UniProtKB Q7L0Q8
Post-translational modification	Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family. UniProtKB Q7L0Q8

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cell projection Golgi apparatus Membrane
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Inferred from direct assay Ref.1. Source: MGI Rac protein signal transduction Inferred from direct assay Ref.1. Source: MGI actin cytoskeleton organization Inferred from direct assay Ref.1. Source: MGI regulation of cell shape Inferred from direct assay Ref.1. Source: MGI
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell projection Inferred from electronic annotation. Source: UniProtKB-KW focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell podosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from mutant phenotype Ref.1. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Rho-related GTP-binding protein RhoU		PRO_0000326436
Regions
	Nucleotide binding	59 – 66	8	GTP By similarity UniProtKB Q7L0Q8
	Nucleotide binding	106 – 110	5	GTP By similarity UniProtKB Q7L0Q8
	Nucleotide binding	164 – 167	4	GTP By similarity UniProtKB P61586
Amino acid modifications
	Lipidation	259	1	S-palmitoyl cysteine By similarity UniProtKB Q7L0Q8

Sequences

	Sequence		Length	Mass (Da)
	Q9EQT3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 8EC4250071E75B14	FASTA	261	28,354
	10 20 30 40 50 60 MAPQQGRPAL PARCEPPAAP PVPPRRERGG RGARGPGVSG GRGRAGGAEG RGVKCVLVGD 70 80 90 100 110 120 GAVGKTSLVV SYTTNGYPTE YIPTAFDNFS AVVSVDGRPV RLQLCDTAGQ DEFDKLRPLC 130 140 150 160 170 180 YTNTDIFLLC FSVVSPTSFQ NVGEKWVPEI RRHCPKAPII LVGTQSDLRE DVKVLIELDK 190 200 210 220 230 240 CKEKPVPEEA AKLCAEEVKA VSYIECSALT QKNLKEVFDA AIVAGIQHSD SQLQPKKSKS 250 260 RTPDKVRDLS KSWWRKYCCL A

References

[1]	"Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1." Tao W., Pennica D., Xu L., Kalejta R.F., Levine A.J. Genes Dev. 15:1796-1807(2001) [PubMed: 11459829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Novel human, mouse and xenopus genes encoding a member of the RAS superfamily of low-molecular-weight GTP-binding proteins and its downregulation in W/WV mouse jejunum." Daigo Y., Takayama I., Ponder B.A.J., Caldas C., Ward S.M., Sanders K.M., Fujino M.A. J. Gastroenterol. Hepatol. 19:211-217(2004) [PubMed: 14731133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF378088 mRNA. Translation: AAK83341.1. AB051827 mRNA. Translation: BAB18639.1.
IPI	IPI00111517.
RefSeq	NP_598716.1. NM_133955.4.
UniGene	Mm.168257.
3D structure databases
HSSP	HSSP built from PDB template 2C2H based on UniProtKB P60763.
ProteinModelPortal	Q9EQT3.
SMR	Q9EQT3. Positions 52-230.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9EQT3.
PTM databases
PhosphoSite	Q9EQT3.
Proteomic databases
PRIDE	Q9EQT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000045487; ENSMUSP00000038915; ENSMUSG00000039960.
GeneID	69581.
KEGG	mmu:69581.
UCSC	uc009nwm.1. mouse.
Organism-specific databases
CTD	58480.
MGI	MGI:1916831. Rhou.
Phylogenomic databases
GeneTree	ENSGT00560000076971.
HOGENOM	HBG745225.
HOVERGEN	HBG009351.
InParanoid	Q9EQT3.
OMA	EIRRHCP.
OrthoDB	EOG4DFPPJ.
PhylomeDB	Q9EQT3.
Gene expression databases
ArrayExpress	Q9EQT3.
Bgee	Q9EQT3.
CleanEx	MM_RHOU.
Genevestigator	Q9EQT3.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
KO	K07865.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	329836.
SOURCE	Search...

Entry information

Entry name

RHOU_MOUSE

Accession

Primary (citable) accession number: Q9EQT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program