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Protein

Rho-related GTP-binding protein RhoU

Gene

Rhou

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (By similarity).By similarity

Cofactori

Mg2+By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 66GTPBy similarity8
Nucleotide bindingi106 – 110GTPBy similarity5
Nucleotide bindingi164 – 167GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • cytoskeleton organization Source: UniProtKB
  • G1/S transition of mitotic cell cycle Source: MGI
  • positive regulation of protein targeting to mitochondrion Source: MGI
  • Rac protein signal transduction Source: MGI
  • regulation of cell shape Source: MGI
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoU
Alternative name(s):
Rho GTPase-like protein ARHU
Wnt-1 responsive Cdc42 homolog 1
Short name:
WRCH-1
Gene namesi
Name:RhouImported
Synonyms:ArhuImported, G28k, Wrch1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1916831. Rhou.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Golgi apparatus membrane By similarity; Lipid-anchor By similarity
  • Cell junctionfocal adhesion By similarity
  • Cell projectionpodosome By similarity

  • Note: Localizes to podosomes in SRC-transformed cells.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264361 – 261Rho-related GTP-binding protein RhoUAdd BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi259S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9EQT3.
PaxDbiQ9EQT3.
PRIDEiQ9EQT3.

PTM databases

iPTMnetiQ9EQT3.
PhosphoSitePlusiQ9EQT3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039960.
CleanExiMM_RHOU.
GenevisibleiQ9EQT3. MM.

Interactioni

Subunit structurei

Interacts with PAK3 (By similarity). Interacts with ARHGAP30 in a GTP-independent manner. In its GTP-loaded conformation, interacts with ARHGAP31. Interacts with PTK2B/PYK2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038915.

Structurei

3D structure databases

ProteinModelPortaliQ9EQT3.
SMRiQ9EQT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.By similarity

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ9EQT3.
KOiK07865.
OMAiKKSKCRT.
OrthoDBiEOG091G0E4F.
PhylomeDBiQ9EQT3.
TreeFamiTF321839.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQQGRPAL PARCEPPAAP PVPPRRERGG RGARGPGVSG GRGRAGGAEG
60 70 80 90 100
RGVKCVLVGD GAVGKTSLVV SYTTNGYPTE YIPTAFDNFS AVVSVDGRPV
110 120 130 140 150
RLQLCDTAGQ DEFDKLRPLC YTNTDIFLLC FSVVSPTSFQ NVGEKWVPEI
160 170 180 190 200
RRHCPKAPII LVGTQSDLRE DVKVLIELDK CKEKPVPEEA AKLCAEEVKA
210 220 230 240 250
VSYIECSALT QKNLKEVFDA AIVAGIQHSD SQLQPKKSKS RTPDKVRDLS
260
KSWWRKYCCL A
Length:261
Mass (Da):28,354
Last modified:March 1, 2001 - v1
Checksum:i8EC4250071E75B14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378088 mRNA. Translation: AAK83341.1.
AB051827 mRNA. Translation: BAB18639.1.
CCDSiCCDS22762.1.
RefSeqiNP_598716.1. NM_133955.4.
UniGeneiMm.168257.

Genome annotation databases

EnsembliENSMUST00000045487; ENSMUSP00000038915; ENSMUSG00000039960.
GeneIDi69581.
KEGGimmu:69581.
UCSCiuc009nwm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378088 mRNA. Translation: AAK83341.1.
AB051827 mRNA. Translation: BAB18639.1.
CCDSiCCDS22762.1.
RefSeqiNP_598716.1. NM_133955.4.
UniGeneiMm.168257.

3D structure databases

ProteinModelPortaliQ9EQT3.
SMRiQ9EQT3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038915.

PTM databases

iPTMnetiQ9EQT3.
PhosphoSitePlusiQ9EQT3.

Proteomic databases

MaxQBiQ9EQT3.
PaxDbiQ9EQT3.
PRIDEiQ9EQT3.

Protocols and materials databases

DNASUi69581.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045487; ENSMUSP00000038915; ENSMUSG00000039960.
GeneIDi69581.
KEGGimmu:69581.
UCSCiuc009nwm.1. mouse.

Organism-specific databases

CTDi58480.
MGIiMGI:1916831. Rhou.

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ9EQT3.
KOiK07865.
OMAiKKSKCRT.
OrthoDBiEOG091G0E4F.
PhylomeDBiQ9EQT3.
TreeFamiTF321839.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiRhou. mouse.
PROiQ9EQT3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039960.
CleanExiMM_RHOU.
GenevisibleiQ9EQT3. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHOU_MOUSE
AccessioniPrimary (citable) accession number: Q9EQT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.