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Q9EQR5

- LIME1_MOUSE

UniProt

Q9EQR5 - LIME1_MOUSE

Protein

Lck-interacting transmembrane adapter 1

Gene

Lime1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.2 Publications

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. protein kinase binding Source: MGI

    GO - Biological processi

    1. B cell receptor signaling pathway Source: MGI
    2. regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
    3. regulation of MAP kinase activity Source: MGI
    4. regulation of NF-kappaB import into nucleus Source: MGI
    5. regulation of phosphatidylinositol 3-kinase signaling Source: MGI
    6. regulation of release of sequestered calcium ion into cytosol Source: MGI
    7. regulation of transcription from RNA polymerase II promoter Source: MGI
    8. T cell receptor signaling pathway Source: InterPro

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lck-interacting transmembrane adapter 1
    Alternative name(s):
    Lck-interacting molecule
    Gene namesi
    Name:Lime1
    Synonyms:Lime
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1919949. Lime1.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type III membrane protein 1 Publication
    Note: Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell.

    GO - Cellular componenti

    1. B cell receptor complex Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi28 – 281C → S: Abolishes palmitoylation and lipid raft localization; when associated with S-31. 1 Publication
    Mutagenesisi31 – 311C → S: Abolishes palmitoylation and lipid raft localization; when associated with S-28. 1 Publication
    Mutagenesisi137 – 1371Y → F: Reduces GRB2 binding. 1 Publication
    Mutagenesisi242 – 2421Y → F: Abolishes LCK, PIK3R1 and LYN binding; when associated with F-261. 2 Publications
    Mutagenesisi261 – 2611Y → F: Strongly reduces PLCG2 binding. Abolishes LCK, PIK3R1 and LYN binding; when associated with F-242. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Lck-interacting transmembrane adapter 1PRO_0000083333Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi28 – 281S-palmitoyl cysteine1 Publication
    Lipidationi31 – 311S-palmitoyl cysteine1 Publication
    Modified residuei137 – 1371Phosphotyrosine1 Publication
    Modified residuei207 – 2071PhosphotyrosineBy similarity
    Modified residuei242 – 2421Phosphotyrosine; by LYN or LCK2 Publications
    Modified residuei261 – 2611Phosphotyrosine; by LYN or LCK2 Publications

    Post-translational modificationi

    Palmitoylation of Cys-28 and Cys-31 is required for raft targeting.1 Publication
    Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins.2 Publications

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ9EQR5.
    PRIDEiQ9EQR5.

    PTM databases

    PhosphoSiteiQ9EQR5.

    Expressioni

    Tissue specificityi

    Expressed in spleen and lung. Present in primary B-cells and peripheral T-cells (at protein level).2 Publications

    Inductioni

    Up-regulated in T-cells following TCR engagement.1 Publication

    Gene expression databases

    BgeeiQ9EQR5.
    CleanExiMM_LIME1.
    GenevestigatoriQ9EQR5.

    Interactioni

    Subunit structurei

    When phosphorylated in response to TCR stimulation and/or CD4 costimulation, interacts with LCK, CSK, FYN, PTPN11/SHP2, GRB2, PIK3R1 and GRAP2 By similarity. When phosphorylated in response to BCR activation, interacts with LYN, PIK3R1, PLCG2 and GRB2.By similarity2 Publications

    Protein-protein interaction databases

    IntActiQ9EQR5. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EQR5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77ExtracellularSequence Analysis
    Topological domaini29 – 269241CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni137 – 1404Interaction with GRB2
    Regioni175 – 1784Interaction with CSKBy similarity
    Regioni207 – 2104Interaction with CSKBy similarity
    Regioni242 – 2454Interaction with LCK and PIK3R1
    Regioni261 – 2644Interaction with LCK, PLCG2 and PIK3R1

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG241375.
    GeneTreeiENSGT00510000050080.
    HOGENOMiHOG000231956.
    HOVERGENiHBG080499.
    OMAiVAEYACI.
    PhylomeDBiQ9EQR5.
    TreeFamiTF337416.

    Family and domain databases

    InterProiIPR026072. Lime1.
    [Graphical view]
    PfamiPF15332. LIME1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EQR5-1 [UniParc]FASTAAdd to Basket

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    MRPPVPSAPL ALWVLGCFSL LLWLWALCTA CHRKRAQRQQ TGLQDSLVPV    50
    EMPLLRQTHL CSLSKSDTRL HELHRGPRSS IAPRPASMDL LHPRWLEMSR 100
    GSTRSQVPNS AFPPRQLPRA PPAAPATAPS TSSEATYSNV GLAAIPRASL 150
    AASPVVWAGT QLTISCARLG PGAEYACIQK HKGTEQGCQE LQQKAKVIPA 200
    TQMDVLYSRV CKPKRRDPRP VTDQLNLQDG RTSLPLGSDV EYEAINLRGQ 250
    DMKQGPLENV YESIKEMGL 269
    Length:269
    Mass (Da):29,551
    Last modified:March 1, 2001 - v1
    Checksum:iB37A1059EB723FBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF115339 mRNA. Translation: AAG35210.1.
    BC023065 mRNA. Translation: AAH23065.1.
    CCDSiCCDS17211.1.
    RefSeqiNP_076173.1. NM_023684.2.
    UniGeneiMm.440138.

    Genome annotation databases

    EnsembliENSMUST00000048077; ENSMUSP00000045010; ENSMUSG00000090077.
    GeneIDi72699.
    KEGGimmu:72699.
    UCSCiuc008omd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF115339 mRNA. Translation: AAG35210.1 .
    BC023065 mRNA. Translation: AAH23065.1 .
    CCDSi CCDS17211.1.
    RefSeqi NP_076173.1. NM_023684.2.
    UniGenei Mm.440138.

    3D structure databases

    ProteinModelPortali Q9EQR5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9EQR5. 1 interaction.

    PTM databases

    PhosphoSitei Q9EQR5.

    Proteomic databases

    PaxDbi Q9EQR5.
    PRIDEi Q9EQR5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000048077 ; ENSMUSP00000045010 ; ENSMUSG00000090077 .
    GeneIDi 72699.
    KEGGi mmu:72699.
    UCSCi uc008omd.1. mouse.

    Organism-specific databases

    CTDi 54923.
    MGIi MGI:1919949. Lime1.

    Phylogenomic databases

    eggNOGi NOG241375.
    GeneTreei ENSGT00510000050080.
    HOGENOMi HOG000231956.
    HOVERGENi HBG080499.
    OMAi VAEYACI.
    PhylomeDBi Q9EQR5.
    TreeFami TF337416.

    Miscellaneous databases

    NextBioi 336761.
    PROi Q9EQR5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9EQR5.
    CleanExi MM_LIME1.
    Genevestigatori Q9EQR5.

    Family and domain databases

    InterProi IPR026072. Lime1.
    [Graphical view ]
    Pfami PF15332. LIME1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
      Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y.
      J. Exp. Med. 198:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH LCK; PTPN11; GRB2; PIK3R1 AND GRAP2, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF CYS-28; CYS-31; TYR-242 AND TYR-261, PALMITOYLATION AT CYS-28 AND CYS-31, PHOSPHORYLATION AT TYR-242 AND TYR-261, SUBCELLULAR LOCATION, FUNCTION.
      Tissue: Lung.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    3. "LIME acts as a transmembrane adapter mediating BCR-dependent B-cell activation."
      Ahn E., Lee H., Yun Y.
      Blood 107:1521-1527(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION, INTERACTION WITH PIK3R1; LYN; PLCG2 AND GRB2, MUTAGENESIS OF TYR-137; TYR-242 AND TYR-261, FUNCTION.

    Entry informationi

    Entry nameiLIME1_MOUSE
    AccessioniPrimary (citable) accession number: Q9EQR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3