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Protein

Lck-interacting transmembrane adapter 1

Gene

Lime1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.2 Publications

GO - Molecular functioni

  1. protein kinase binding Source: MGI

GO - Biological processi

  1. B cell receptor signaling pathway Source: MGI
  2. regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  3. regulation of MAP kinase activity Source: MGI
  4. regulation of NF-kappaB import into nucleus Source: MGI
  5. regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  6. regulation of release of sequestered calcium ion into cytosol Source: MGI
  7. regulation of transcription from RNA polymerase II promoter Source: MGI
  8. T cell receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Lck-interacting transmembrane adapter 1
Alternative name(s):
Lck-interacting molecule
Gene namesi
Name:Lime1
Synonyms:Lime
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919949. Lime1.

Subcellular locationi

Cell membrane 1 Publication; Single-pass type III membrane protein 1 Publication
Note: Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77ExtracellularSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 269241CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. B cell receptor complex Source: MGI
  2. extracellular space Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281C → S: Abolishes palmitoylation and lipid raft localization; when associated with S-31. 1 Publication
Mutagenesisi31 – 311C → S: Abolishes palmitoylation and lipid raft localization; when associated with S-28. 1 Publication
Mutagenesisi137 – 1371Y → F: Reduces GRB2 binding. 1 Publication
Mutagenesisi242 – 2421Y → F: Abolishes LCK, PIK3R1 and LYN binding; when associated with F-261. 2 Publications
Mutagenesisi261 – 2611Y → F: Strongly reduces PLCG2 binding. Abolishes LCK, PIK3R1 and LYN binding; when associated with F-242. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Lck-interacting transmembrane adapter 1PRO_0000083333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi28 – 281S-palmitoyl cysteine1 Publication
Lipidationi31 – 311S-palmitoyl cysteine1 Publication
Modified residuei137 – 1371Phosphotyrosine1 Publication
Modified residuei207 – 2071PhosphotyrosineBy similarity
Modified residuei242 – 2421Phosphotyrosine; by LYN or LCK1 Publication
Modified residuei261 – 2611Phosphotyrosine; by LYN or LCK1 Publication
Modified residuei263 – 2631PhosphoserineBy similarity

Post-translational modificationi

Palmitoylation of Cys-28 and Cys-31 is required for raft targeting.1 Publication
Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins.2 Publications

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9EQR5.
PRIDEiQ9EQR5.

PTM databases

PhosphoSiteiQ9EQR5.

Expressioni

Tissue specificityi

Expressed in spleen and lung. Present in primary B-cells and peripheral T-cells (at protein level).2 Publications

Inductioni

Up-regulated in T-cells following TCR engagement.1 Publication

Gene expression databases

BgeeiQ9EQR5.
CleanExiMM_LIME1.
ExpressionAtlasiQ9EQR5. baseline and differential.
GenevestigatoriQ9EQR5.

Interactioni

Subunit structurei

When phosphorylated in response to TCR stimulation and/or CD4 costimulation, interacts with LCK, CSK, FYN, PTPN11/SHP2, GRB2, PIK3R1 and GRAP2 (By similarity). When phosphorylated in response to BCR activation, interacts with LYN, PIK3R1, PLCG2 and GRB2.By similarity2 Publications

Protein-protein interaction databases

IntActiQ9EQR5. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9EQR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1404Interaction with GRB2
Regioni175 – 1784Interaction with CSKBy similarity
Regioni207 – 2104Interaction with CSKBy similarity
Regioni242 – 2454Interaction with LCK and PIK3R1
Regioni261 – 2644Interaction with LCK, PLCG2 and PIK3R1

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG241375.
GeneTreeiENSGT00510000050080.
HOGENOMiHOG000231956.
HOVERGENiHBG080499.
InParanoidiQ9EQR5.
OMAiVAEYACI.
PhylomeDBiQ9EQR5.
TreeFamiTF337416.

Family and domain databases

InterProiIPR026072. Lime1.
[Graphical view]
PfamiPF15332. LIME1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPVPSAPL ALWVLGCFSL LLWLWALCTA CHRKRAQRQQ TGLQDSLVPV
60 70 80 90 100
EMPLLRQTHL CSLSKSDTRL HELHRGPRSS IAPRPASMDL LHPRWLEMSR
110 120 130 140 150
GSTRSQVPNS AFPPRQLPRA PPAAPATAPS TSSEATYSNV GLAAIPRASL
160 170 180 190 200
AASPVVWAGT QLTISCARLG PGAEYACIQK HKGTEQGCQE LQQKAKVIPA
210 220 230 240 250
TQMDVLYSRV CKPKRRDPRP VTDQLNLQDG RTSLPLGSDV EYEAINLRGQ
260
DMKQGPLENV YESIKEMGL
Length:269
Mass (Da):29,551
Last modified:February 28, 2001 - v1
Checksum:iB37A1059EB723FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115339 mRNA. Translation: AAG35210.1.
BC023065 mRNA. Translation: AAH23065.1.
CCDSiCCDS17211.1.
RefSeqiNP_076173.1. NM_023684.2.
UniGeneiMm.440138.

Genome annotation databases

EnsembliENSMUST00000048077; ENSMUSP00000045010; ENSMUSG00000090077.
GeneIDi72699.
KEGGimmu:72699.
UCSCiuc008omd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115339 mRNA. Translation: AAG35210.1.
BC023065 mRNA. Translation: AAH23065.1.
CCDSiCCDS17211.1.
RefSeqiNP_076173.1. NM_023684.2.
UniGeneiMm.440138.

3D structure databases

ProteinModelPortaliQ9EQR5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EQR5. 1 interaction.

PTM databases

PhosphoSiteiQ9EQR5.

Proteomic databases

PaxDbiQ9EQR5.
PRIDEiQ9EQR5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048077; ENSMUSP00000045010; ENSMUSG00000090077.
GeneIDi72699.
KEGGimmu:72699.
UCSCiuc008omd.1. mouse.

Organism-specific databases

CTDi54923.
MGIiMGI:1919949. Lime1.

Phylogenomic databases

eggNOGiNOG241375.
GeneTreeiENSGT00510000050080.
HOGENOMiHOG000231956.
HOVERGENiHBG080499.
InParanoidiQ9EQR5.
OMAiVAEYACI.
PhylomeDBiQ9EQR5.
TreeFamiTF337416.

Miscellaneous databases

NextBioi336761.
PROiQ9EQR5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EQR5.
CleanExiMM_LIME1.
ExpressionAtlasiQ9EQR5. baseline and differential.
GenevestigatoriQ9EQR5.

Family and domain databases

InterProiIPR026072. Lime1.
[Graphical view]
PfamiPF15332. LIME1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation."
    Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y.
    J. Exp. Med. 198:1463-1473(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH LCK; PTPN11; GRB2; PIK3R1 AND GRAP2, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF CYS-28; CYS-31; TYR-242 AND TYR-261, PALMITOYLATION AT CYS-28 AND CYS-31, PHOSPHORYLATION AT TYR-242 AND TYR-261, SUBCELLULAR LOCATION, FUNCTION.
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "LIME acts as a transmembrane adapter mediating BCR-dependent B-cell activation."
    Ahn E., Lee H., Yun Y.
    Blood 107:1521-1527(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION, INTERACTION WITH PIK3R1; LYN; PLCG2 AND GRB2, MUTAGENESIS OF TYR-137; TYR-242 AND TYR-261, FUNCTION.

Entry informationi

Entry nameiLIME1_MOUSE
AccessioniPrimary (citable) accession number: Q9EQR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2005
Last sequence update: February 28, 2001
Last modified: March 3, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.