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Protein

Lck-interacting transmembrane adapter 1

Gene

Lime1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.2 Publications

GO - Molecular functioni

  • protein kinase binding Source: MGI

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • B cell receptor signaling pathway Source: MGI
  • regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • regulation of MAP kinase activity Source: MGI
  • regulation of NF-kappaB import into nucleus Source: MGI
  • regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  • regulation of release of sequestered calcium ion into cytosol Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • spliceosomal complex disassembly Source: GO_Central
  • T cell receptor signaling pathway Source: InterPro

Keywordsi

Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Lck-interacting transmembrane adapter 1
Alternative name(s):
Lck-interacting molecule
Gene namesi
Name:Lime1
Synonyms:Lime
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919949 Lime1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7ExtracellularSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 269CytoplasmicSequence analysisAdd BLAST241

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28C → S: Abolishes palmitoylation and lipid raft localization; when associated with S-31. 1 Publication1
Mutagenesisi31C → S: Abolishes palmitoylation and lipid raft localization; when associated with S-28. 1 Publication1
Mutagenesisi137Y → F: Reduces GRB2 binding. 1 Publication1
Mutagenesisi242Y → F: Abolishes LCK, PIK3R1 and LYN binding; when associated with F-261. 2 Publications1
Mutagenesisi261Y → F: Strongly reduces PLCG2 binding. Abolishes LCK, PIK3R1 and LYN binding; when associated with F-242. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833331 – 269Lck-interacting transmembrane adapter 1Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi28S-palmitoyl cysteine1 Publication1
Lipidationi31S-palmitoyl cysteine1 Publication1
Modified residuei137Phosphotyrosine1 Publication1
Modified residuei175PhosphotyrosineBy similarity1
Modified residuei207PhosphotyrosineBy similarity1
Modified residuei242Phosphotyrosine; by LYN or LCK1 Publication1
Modified residuei261Phosphotyrosine; by LYN or LCK1 Publication1
Modified residuei263PhosphoserineBy similarity1

Post-translational modificationi

Palmitoylation of Cys-28 and Cys-31 is required for raft targeting.1 Publication
Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins.2 Publications

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ9EQR5
PaxDbiQ9EQR5
PRIDEiQ9EQR5

PTM databases

iPTMnetiQ9EQR5
PhosphoSitePlusiQ9EQR5

Expressioni

Tissue specificityi

Expressed in spleen and lung. Present in primary B-cells and peripheral T-cells (at protein level).2 Publications

Inductioni

Up-regulated in T-cells following TCR engagement.1 Publication

Gene expression databases

BgeeiENSMUSG00000090077
CleanExiMM_LIME1
ExpressionAtlasiQ9EQR5 baseline and differential
GenevisibleiQ9EQR5 MM

Interactioni

Subunit structurei

When phosphorylated in response to TCR stimulation and/or CD4 costimulation, interacts with LCK, CSK, FYN, PTPN11/SHP2, GRB2, PIK3R1 and GRAP2 (By similarity). When phosphorylated in response to BCR activation, interacts with LYN, PIK3R1, PLCG2 and GRB2.By similarity2 Publications

GO - Molecular functioni

  • protein kinase binding Source: MGI

Protein-protein interaction databases

IntActiQ9EQR5, 1 interactor
STRINGi10090.ENSMUSP00000045010

Structurei

3D structure databases

ProteinModelPortaliQ9EQR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 140Interaction with GRB24
Regioni175 – 178Interaction with CSKBy similarity4
Regioni207 – 210Interaction with CSKBy similarity4
Regioni242 – 245Interaction with LCK and PIK3R11 Publication4
Regioni261 – 264Interaction with LCK, PLCG2 and PIK3R12 Publications4

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J9JT Eukaryota
ENOG41114H9 LUCA
GeneTreeiENSGT00510000050080
HOGENOMiHOG000231956
HOVERGENiHBG080499
InParanoidiQ9EQR5
OMAiLYSRVCK
OrthoDBiEOG091G0IKW
PhylomeDBiQ9EQR5
TreeFamiTF337416

Family and domain databases

InterProiView protein in InterPro
IPR026072 Lime1
PfamiView protein in Pfam
PF15332 LIME1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9EQR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPPVPSAPL ALWVLGCFSL LLWLWALCTA CHRKRAQRQQ TGLQDSLVPV
60 70 80 90 100
EMPLLRQTHL CSLSKSDTRL HELHRGPRSS IAPRPASMDL LHPRWLEMSR
110 120 130 140 150
GSTRSQVPNS AFPPRQLPRA PPAAPATAPS TSSEATYSNV GLAAIPRASL
160 170 180 190 200
AASPVVWAGT QLTISCARLG PGAEYACIQK HKGTEQGCQE LQQKAKVIPA
210 220 230 240 250
TQMDVLYSRV CKPKRRDPRP VTDQLNLQDG RTSLPLGSDV EYEAINLRGQ
260
DMKQGPLENV YESIKEMGL
Length:269
Mass (Da):29,551
Last modified:March 1, 2001 - v1
Checksum:iB37A1059EB723FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115339 mRNA Translation: AAG35210.1
BC023065 mRNA Translation: AAH23065.1
CCDSiCCDS17211.1
RefSeqiNP_076173.1, NM_023684.2
UniGeneiMm.440138

Genome annotation databases

EnsembliENSMUST00000048077; ENSMUSP00000045010; ENSMUSG00000090077
GeneIDi72699
KEGGimmu:72699
UCSCiuc008omd.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLIME1_MOUSE
AccessioniPrimary (citable) accession number: Q9EQR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health