Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9EQP2 (EHD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
EH domain-containing protein 4
Alternative name(s):
PAST homolog 2
Short name=mPAST2
Gene names
Name:Ehd4
Synonyms:Past2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in early endosomal transport. Ref.3

Subunit structure

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3 By similarity.

Subcellular location

Cell membrane. Early endosome membrane; Peripheral membrane protein By similarity. Recycling endosome membrane; Peripheral membrane protein By similarity Ref.4.

Domain

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins By similarity.

Sequence similarities

Contains 1 EF-hand domain.

Contains 1 EH domain.

Ontologies

Keywords
   Cellular componentCell membrane
Endosome
Membrane
   LigandATP-binding
Calcium
Metal-binding
Nucleotide-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

endocytic recycling

Inferred from sequence or structural similarity. Source: UniProtKB

pinocytosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

protein homooligomerization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of endocytosis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentearly endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from direct assay PubMed 20489164. Source: MGI

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

recycling endosome membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTP binding

Inferred from electronic annotation. Source: InterPro

GTPase activity

Inferred from electronic annotation. Source: InterPro

calcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Pacsin2Q9QY174EBI-491022,EBI-491201From a different organism.
Snap29Q9Z2P62EBI-491022,EBI-492883From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 541541EH domain-containing protein 4
PRO_0000146115

Regions

Domain447 – 53589EH
Domain479 – 51436EF-hand
Nucleotide binding68 – 758ATP By similarity
Calcium binding492 – 50312 By similarity

Sites

Binding site2231ATP By similarity
Binding site2611ATP By similarity

Amino acid modifications

Modified residue4511Phosphotyrosine By similarity
Modified residue4591Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9EQP2 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: D34C28F046D740E1

FASTA54161,481
        10         20         30         40         50         60 
MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS PALEDADFEN 

        70         80         90        100        110        120 
KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF IAVMYGETEG STPGNALVVD 

       130        140        150        160        170        180 
PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK SISIIDSPGI LSGEKQRISR GYDFCQVLQW 

       190        200        210        220        230        240 
FAERVDRIIL LFDAHKLDIS DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM 

       250        260        270        280        290        300 
WSLGKVINTP EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL 

       310        320        330        340        350        360 
IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ ISAGDFPEVK 

       370        380        390        400        410        420 
AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI SQEEMNMPTQ MVQGGAFDGT 

       430        440        450        460        470        480 
TEGPFNQGYG EGAKEGADEE EWVVAKDKPV YDELFYTLSP INGKISGVNA KKEMVTSKLP 

       490        500        510        520        530        540 
NSVLGKIWKL ADCDCDGMLD EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA 


D 

« Hide

References

« Hide 'large scale' references
[1]"MPAST2, a novel murine isoform of the PAST protein family."
Plomann M., Behrendt D., Ritter B., Modregger J., Halbach A., Paulsson M.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary gland.
[3]"EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling."
Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B.
Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion."
Posey A.D. Jr., Pytel P., Gardikiotes K., Demonbreun A.R., Rainey M., George M., Band H., McNally E.M.
J. Biol. Chem. 286:7379-7388(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF173639 mRNA. Translation: AAG45672.1.
BC007480 mRNA. Translation: AAH07480.1.
RefSeqNP_598599.2. NM_133838.4.
UniGeneMm.132226.

3D structure databases

ProteinModelPortalQ9EQP2.
SMRQ9EQP2. Positions 22-535.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid221151. 1 interaction.
IntActQ9EQP2. 3 interactions.
MINTMINT-4094173.

PTM databases

PhosphoSiteQ9EQP2.

Proteomic databases

PaxDbQ9EQP2.
PRIDEQ9EQP2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028755; ENSMUSP00000028755; ENSMUSG00000027293.
GeneID98878.
KEGGmmu:98878.
UCSCuc008lvd.1. mouse.

Organism-specific databases

CTD30844.
MGIMGI:1919619. Ehd4.

Phylogenomic databases

eggNOGNOG136252.
GeneTreeENSGT00700000104190.
HOVERGENHBG018183.
InParanoidQ9EQP2.
KOK12477.
OMAMPNQVLQ.
OrthoDBEOG757CX9.
PhylomeDBQ9EQP2.
TreeFamTF314429.

Gene expression databases

ArrayExpressQ9EQP2.
BgeeQ9EQP2.
CleanExMM_EHD4.
GenevestigatorQ9EQP2.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProIPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00350. Dynamin_N. 1 hit.
[Graphical view]
SMARTSM00027. EH. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEHD4. mouse.
NextBio353697.
PROQ9EQP2.
SOURCESearch...

Entry information

Entry nameEHD4_MOUSE
AccessionPrimary (citable) accession number: Q9EQP2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot