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Q9EQP2

- EHD4_MOUSE

UniProt

Q9EQP2 - EHD4_MOUSE

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Protein
EH domain-containing protein 4
Gene
Ehd4, Past2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in early endosomal transport.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231ATP By similarity
Binding sitei261 – 2611ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 758ATP By similarity
Calcium bindingi492 – 50312 By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: InterPro
  3. GTPase activity Source: InterPro
  4. calcium ion binding Source: InterPro
  5. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. cellular response to growth factor stimulus Source: Ensembl
  2. endocytic recycling Source: UniProtKB
  3. pinocytosis Source: Ensembl
  4. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  5. protein homooligomerization Source: UniProtKB
  6. regulation of endocytosis Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 4
Alternative name(s):
PAST homolog 2
Short name:
mPAST2
Gene namesi
Name:Ehd4
Synonyms:Past2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1919619. Ehd4.

Subcellular locationi

Cell membrane. Early endosome membrane; Peripheral membrane protein By similarity. Recycling endosome membrane; Peripheral membrane protein By similarity 1 Publication

GO - Cellular componenti

  1. early endosome membrane Source: UniProtKB-SubCell
  2. endoplasmic reticulum Source: Ensembl
  3. perinuclear region of cytoplasm Source: MGI
  4. plasma membrane Source: UniProtKB-SubCell
  5. recycling endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 541541EH domain-containing protein 4
PRO_0000146115Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei451 – 4511Phosphotyrosine By similarity
Modified residuei459 – 4591Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9EQP2.
PaxDbiQ9EQP2.
PRIDEiQ9EQP2.

PTM databases

PhosphoSiteiQ9EQP2.

Expressioni

Gene expression databases

ArrayExpressiQ9EQP2.
BgeeiQ9EQP2.
CleanExiMM_EHD4.
GenevestigatoriQ9EQP2.

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Pacsin2Q9QY174EBI-491022,EBI-491201From a different organism.
Snap29Q9Z2P62EBI-491022,EBI-492883From a different organism.

Protein-protein interaction databases

BioGridi221151. 1 interaction.
IntActiQ9EQP2. 3 interactions.
MINTiMINT-4094173.

Structurei

3D structure databases

ProteinModelPortaliQ9EQP2.
SMRiQ9EQP2. Positions 1-535.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 289232Dynamin-type G
Add
BLAST
Domaini447 – 53589EH
Add
BLAST
Domaini479 – 51436EF-hand
Add
BLAST

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins By similarity.

Sequence similaritiesi

Contains 1 EF-hand domain.
Contains 1 EH domain.

Phylogenomic databases

eggNOGiNOG136252.
GeneTreeiENSGT00700000104190.
HOVERGENiHBG018183.
InParanoidiQ9EQP2.
KOiK12477.
OMAiMPNQVLQ.
OrthoDBiEOG757CX9.
PhylomeDBiQ9EQP2.
TreeFamiTF314429.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQP2-1 [UniParc]FASTAAdd to Basket

« Hide

MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS    50
PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF 100
IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK 150
SISIIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS 200
DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP 250
EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL 300
IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ 350
ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI 400
SQEEMNMPTQ MVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV 450
YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD 500
EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA D 541
Length:541
Mass (Da):61,481
Last modified:March 1, 2001 - v1
Checksum:iD34C28F046D740E1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF173639 mRNA. Translation: AAG45672.1.
BC007480 mRNA. Translation: AAH07480.1.
CCDSiCCDS16615.1.
RefSeqiNP_598599.2. NM_133838.4.
UniGeneiMm.132226.

Genome annotation databases

EnsembliENSMUST00000028755; ENSMUSP00000028755; ENSMUSG00000027293.
GeneIDi98878.
KEGGimmu:98878.
UCSCiuc008lvd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF173639 mRNA. Translation: AAG45672.1 .
BC007480 mRNA. Translation: AAH07480.1 .
CCDSi CCDS16615.1.
RefSeqi NP_598599.2. NM_133838.4.
UniGenei Mm.132226.

3D structure databases

ProteinModelPortali Q9EQP2.
SMRi Q9EQP2. Positions 1-535.
ModBasei Search...

Protein-protein interaction databases

BioGridi 221151. 1 interaction.
IntActi Q9EQP2. 3 interactions.
MINTi MINT-4094173.

PTM databases

PhosphoSitei Q9EQP2.

Proteomic databases

MaxQBi Q9EQP2.
PaxDbi Q9EQP2.
PRIDEi Q9EQP2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028755 ; ENSMUSP00000028755 ; ENSMUSG00000027293 .
GeneIDi 98878.
KEGGi mmu:98878.
UCSCi uc008lvd.1. mouse.

Organism-specific databases

CTDi 30844.
MGIi MGI:1919619. Ehd4.

Phylogenomic databases

eggNOGi NOG136252.
GeneTreei ENSGT00700000104190.
HOVERGENi HBG018183.
InParanoidi Q9EQP2.
KOi K12477.
OMAi MPNQVLQ.
OrthoDBi EOG757CX9.
PhylomeDBi Q9EQP2.
TreeFami TF314429.

Miscellaneous databases

ChiTaRSi EHD4. mouse.
NextBioi 353697.
PROi Q9EQP2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9EQP2.
Bgeei Q9EQP2.
CleanExi MM_EHD4.
Genevestigatori Q9EQP2.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProi IPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00350. Dynamin_N. 1 hit.
[Graphical view ]
SMARTi SM00027. EH. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MPAST2, a novel murine isoform of the PAST protein family."
    Plomann M., Behrendt D., Ritter B., Modregger J., Halbach A., Paulsson M.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  3. "EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling."
    Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B.
    Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion."
    Posey A.D. Jr., Pytel P., Gardikiotes K., Demonbreun A.R., Rainey M., George M., Band H., McNally E.M.
    J. Biol. Chem. 286:7379-7388(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiEHD4_MOUSE
AccessioniPrimary (citable) accession number: Q9EQP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi