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Protein

EH domain-containing protein 4

Gene

Ehd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223ATPBy similarity1
Binding sitei261ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 75ATPBy similarity8
Calcium bindingi492 – 503PROSITE-ProRule annotationBy similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 4Curated
Alternative name(s):
PAST homolog 2Curated
Short name:
mPAST21 Publication
Gene namesi
Name:Ehd4Imported
Synonyms:Past21 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919619. Ehd4.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001461151 – 541EH domain-containing protein 4Add BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei15PhosphoserineCombined sources1
Modified residuei162PhosphoserineBy similarity1
Modified residuei451PhosphotyrosineBy similarity1
Modified residuei459PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9EQP2.
MaxQBiQ9EQP2.
PaxDbiQ9EQP2.
PRIDEiQ9EQP2.

PTM databases

iPTMnetiQ9EQP2.
PhosphoSitePlusiQ9EQP2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027293.
CleanExiMM_EHD4.
ExpressionAtlasiQ9EQP2. baseline and differential.
GenevisibleiQ9EQP2. MM.

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221151. 1 interactor.
IntActiQ9EQP2. 4 interactors.
MINTiMINT-4094173.
STRINGi10090.ENSMUSP00000028755.

Structurei

Secondary structure

1541
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 35Combined sources14
Helixi37 – 42Combined sources6
Helixi45 – 48Combined sources4
Helixi55 – 57Combined sources3
Beta strandi63 – 69Combined sources7
Helixi74 – 82Combined sources9
Turni95 – 97Combined sources3
Beta strandi100 – 105Combined sources6
Beta strandi107 – 113Combined sources7
Helixi114 – 117Combined sources4
Helixi127 – 130Combined sources4
Turni133 – 135Combined sources3
Helixi136 – 138Combined sources3
Beta strandi139 – 144Combined sources6
Helixi148 – 150Combined sources3
Beta strandi153 – 156Combined sources4
Helixi174 – 184Combined sources11
Beta strandi186 – 193Combined sources8
Helixi194 – 196Combined sources3
Helixi201 – 209Combined sources9
Turni210 – 212Combined sources3
Helixi214 – 216Combined sources3
Beta strandi217 – 222Combined sources6
Helixi229 – 247Combined sources19
Beta strandi255 – 258Combined sources4
Beta strandi261 – 263Combined sources3
Helixi268 – 270Combined sources3
Helixi271 – 319Combined sources49
Beta strandi323 – 325Combined sources3
Helixi327 – 336Combined sources10
Helixi338 – 347Combined sources10
Turni348 – 350Combined sources3
Beta strandi353 – 356Combined sources4
Helixi359 – 366Combined sources8
Helixi371 – 373Combined sources3
Helixi379 – 390Combined sources12
Helixi392 – 401Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MTVX-ray2.79A22-541[»]
5MVFX-ray3.27A22-541[»]
ProteinModelPortaliQ9EQP2.
SMRiQ9EQP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 289Dynamin-type GAdd BLAST232
Domaini447 – 535EHPROSITE-ProRule annotationAdd BLAST89
Domaini479 – 514EF-handPROSITE-ProRule annotationAdd BLAST36

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1954. Eukaryota.
ENOG410XYGB. LUCA.
GeneTreeiENSGT00760000118985.
HOVERGENiHBG018183.
InParanoidiQ9EQP2.
KOiK12477.
OMAiQREHHIS.
OrthoDBiEOG091G04KA.
PhylomeDBiQ9EQP2.
TreeFamiTF314429.

Family and domain databases

InterProiView protein in InterPro
IPR022812. Dynamin_SF.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR029952. EHD4.
IPR031692. EHD_N.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
PANTHERiPTHR11216:SF107. PTHR11216:SF107. 1 hit.
PfamiView protein in Pfam
PF00350. Dynamin_N. 1 hit.
PF12763. EF-hand_4. 1 hit.
PF16880. EHD_N. 1 hit.
SMARTiView protein in SMART
SM00027. EH. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9EQP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS
60 70 80 90 100
PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF
110 120 130 140 150
IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK
160 170 180 190 200
SISIIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS
210 220 230 240 250
DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP
260 270 280 290 300
EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL
310 320 330 340 350
IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ
360 370 380 390 400
ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI
410 420 430 440 450
SQEEMNMPTQ MVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV
460 470 480 490 500
YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD
510 520 530 540
EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA D
Length:541
Mass (Da):61,481
Last modified:March 1, 2001 - v1
Checksum:iD34C28F046D740E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173639 mRNA. Translation: AAG45672.1.
BC007480 mRNA. Translation: AAH07480.1.
CCDSiCCDS16615.1.
RefSeqiNP_598599.2. NM_133838.4.
UniGeneiMm.132226.

Genome annotation databases

EnsembliENSMUST00000028755; ENSMUSP00000028755; ENSMUSG00000027293.
GeneIDi98878.
KEGGimmu:98878.
UCSCiuc008lvd.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiEHD4_MOUSE
AccessioniPrimary (citable) accession number: Q9EQP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: June 7, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families