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Protein

EH domain-containing protein 4

Gene

Ehd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223ATPBy similarity1
Binding sitei261ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 75ATPBy similarity8
Calcium bindingi492 – 503PROSITE-ProRule annotationBy similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 4Curated
Alternative name(s):
PAST homolog 2Curated
Short name:
mPAST21 Publication
Gene namesi
Name:Ehd4Imported
Synonyms:Past21 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919619 Ehd4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001461151 – 541EH domain-containing protein 4Add BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei15PhosphoserineCombined sources1
Modified residuei162PhosphoserineBy similarity1
Modified residuei451PhosphotyrosineBy similarity1
Modified residuei459PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9EQP2
MaxQBiQ9EQP2
PaxDbiQ9EQP2
PRIDEiQ9EQP2

PTM databases

iPTMnetiQ9EQP2
PhosphoSitePlusiQ9EQP2

Expressioni

Gene expression databases

BgeeiENSMUSG00000027293
CleanExiMM_EHD4
ExpressionAtlasiQ9EQP2 baseline and differential
GenevisibleiQ9EQP2 MM

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221151, 3 interactors
IntActiQ9EQP2, 5 interactors
MINTiQ9EQP2
STRINGi10090.ENSMUSP00000028755

Structurei

Secondary structure

1541
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 35Combined sources14
Helixi37 – 42Combined sources6
Helixi45 – 48Combined sources4
Helixi55 – 57Combined sources3
Beta strandi63 – 69Combined sources7
Helixi74 – 82Combined sources9
Turni95 – 97Combined sources3
Beta strandi100 – 105Combined sources6
Beta strandi107 – 113Combined sources7
Helixi114 – 117Combined sources4
Helixi127 – 130Combined sources4
Turni133 – 135Combined sources3
Helixi136 – 138Combined sources3
Beta strandi139 – 144Combined sources6
Helixi148 – 150Combined sources3
Beta strandi153 – 156Combined sources4
Helixi174 – 184Combined sources11
Beta strandi186 – 193Combined sources8
Helixi194 – 196Combined sources3
Helixi201 – 209Combined sources9
Turni210 – 212Combined sources3
Helixi214 – 216Combined sources3
Beta strandi217 – 222Combined sources6
Helixi229 – 247Combined sources19
Beta strandi255 – 258Combined sources4
Beta strandi261 – 263Combined sources3
Helixi268 – 270Combined sources3
Helixi271 – 319Combined sources49
Beta strandi323 – 325Combined sources3
Helixi327 – 336Combined sources10
Helixi338 – 347Combined sources10
Turni348 – 350Combined sources3
Beta strandi353 – 356Combined sources4
Helixi359 – 366Combined sources8
Helixi371 – 373Combined sources3
Helixi379 – 390Combined sources12
Helixi392 – 401Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MTVX-ray2.79A22-541[»]
5MVFX-ray3.27A22-541[»]
ProteinModelPortaliQ9EQP2
SMRiQ9EQP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 289Dynamin-type GPROSITE-ProRule annotationAdd BLAST232
Domaini447 – 535EHPROSITE-ProRule annotationAdd BLAST89
Domaini479 – 514EF-handPROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 75G1 motifPROSITE-ProRule annotation8
Regioni94 – 95G2 motifPROSITE-ProRule annotation2
Regioni156 – 159G3 motifPROSITE-ProRule annotation4
Regioni222 – 225G4 motifPROSITE-ProRule annotation4
Regioni246G5 motifPROSITE-ProRule annotation1

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1954 Eukaryota
ENOG410XYGB LUCA
GeneTreeiENSGT00760000118985
HOVERGENiHBG018183
InParanoidiQ9EQP2
KOiK12477
OMAiGEMYQTV
OrthoDBiEOG091G04KA
PhylomeDBiQ9EQP2
TreeFamiTF314429

Family and domain databases

InterProiView protein in InterPro
IPR022812 Dynamin_SF
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR029952 EHD4
IPR031692 EHD_N
IPR030381 G_DYNAMIN_dom
IPR027417 P-loop_NTPase
PANTHERiPTHR11216:SF57 PTHR11216:SF57, 1 hit
PfamiView protein in Pfam
PF00350 Dynamin_N, 1 hit
PF12763 EF-hand_4, 1 hit
PF16880 EHD_N, 1 hit
SMARTiView protein in SMART
SM00027 EH, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50031 EH, 1 hit
PS51718 G_DYNAMIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9EQP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS
60 70 80 90 100
PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF
110 120 130 140 150
IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK
160 170 180 190 200
SISIIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS
210 220 230 240 250
DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP
260 270 280 290 300
EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL
310 320 330 340 350
IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ
360 370 380 390 400
ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI
410 420 430 440 450
SQEEMNMPTQ MVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV
460 470 480 490 500
YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD
510 520 530 540
EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA D
Length:541
Mass (Da):61,481
Last modified:March 1, 2001 - v1
Checksum:iD34C28F046D740E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173639 mRNA Translation: AAG45672.1
BC007480 mRNA Translation: AAH07480.1
CCDSiCCDS16615.1
RefSeqiNP_598599.2, NM_133838.4
UniGeneiMm.132226

Genome annotation databases

EnsembliENSMUST00000028755; ENSMUSP00000028755; ENSMUSG00000027293
GeneIDi98878
KEGGimmu:98878
UCSCiuc008lvd.1 mouse

Similar proteinsi

Entry informationi

Entry nameiEHD4_MOUSE
AccessioniPrimary (citable) accession number: Q9EQP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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