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Q9EQP2

- EHD4_MOUSE

UniProt

Q9EQP2 - EHD4_MOUSE

Protein

EH domain-containing protein 4

Gene

Ehd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Plays a role in early endosomal transport.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei223 – 2231ATPBy similarity
    Binding sitei261 – 2611ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi68 – 758ATPBy similarity
    Calcium bindingi492 – 50312PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. GTPase activity Source: InterPro
    4. GTP binding Source: InterPro
    5. protein binding Source: IntAct

    GO - Biological processi

    1. cellular response to growth factor stimulus Source: Ensembl
    2. endocytic recycling Source: UniProtKB
    3. pinocytosis Source: Ensembl
    4. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    5. protein homooligomerization Source: UniProtKB
    6. regulation of endocytosis Source: Ensembl

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EH domain-containing protein 4
    Alternative name(s):
    PAST homolog 2
    Short name:
    mPAST2
    Gene namesi
    Name:Ehd4
    Synonyms:Past2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1919619. Ehd4.

    Subcellular locationi

    Cell membrane 1 Publication. Early endosome membrane By similarity; Peripheral membrane protein By similarity. Recycling endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. early endosome membrane Source: UniProtKB-SubCell
    2. endoplasmic reticulum Source: Ensembl
    3. perinuclear region of cytoplasm Source: MGI
    4. plasma membrane Source: UniProtKB-SubCell
    5. recycling endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 541541EH domain-containing protein 4PRO_0000146115Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei451 – 4511PhosphotyrosineBy similarity
    Modified residuei459 – 4591PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9EQP2.
    PaxDbiQ9EQP2.
    PRIDEiQ9EQP2.

    PTM databases

    PhosphoSiteiQ9EQP2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9EQP2.
    BgeeiQ9EQP2.
    CleanExiMM_EHD4.
    GenevestigatoriQ9EQP2.

    Interactioni

    Subunit structurei

    Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Pacsin2Q9QY174EBI-491022,EBI-491201From a different organism.
    Snap29Q9Z2P62EBI-491022,EBI-492883From a different organism.

    Protein-protein interaction databases

    BioGridi221151. 1 interaction.
    IntActiQ9EQP2. 3 interactions.
    MINTiMINT-4094173.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EQP2.
    SMRiQ9EQP2. Positions 1-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 289232Dynamin-type GAdd
    BLAST
    Domaini447 – 53589EHPROSITE-ProRule annotationAdd
    BLAST
    Domaini479 – 51436EF-handPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

    Sequence similaritiesi

    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 EH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG136252.
    GeneTreeiENSGT00700000104190.
    HOVERGENiHBG018183.
    InParanoidiQ9EQP2.
    KOiK12477.
    OMAiMPNQVLQ.
    OrthoDBiEOG757CX9.
    PhylomeDBiQ9EQP2.
    TreeFamiTF314429.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    3.40.50.300. 3 hits.
    InterProiIPR001401. Dynamin_GTPase.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00350. Dynamin_N. 1 hit.
    [Graphical view]
    SMARTiSM00027. EH. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50031. EH. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EQP2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS    50
    PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF 100
    IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK 150
    SISIIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS 200
    DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP 250
    EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL 300
    IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ 350
    ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI 400
    SQEEMNMPTQ MVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV 450
    YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD 500
    EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA D 541
    Length:541
    Mass (Da):61,481
    Last modified:March 1, 2001 - v1
    Checksum:iD34C28F046D740E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF173639 mRNA. Translation: AAG45672.1.
    BC007480 mRNA. Translation: AAH07480.1.
    CCDSiCCDS16615.1.
    RefSeqiNP_598599.2. NM_133838.4.
    UniGeneiMm.132226.

    Genome annotation databases

    EnsembliENSMUST00000028755; ENSMUSP00000028755; ENSMUSG00000027293.
    GeneIDi98878.
    KEGGimmu:98878.
    UCSCiuc008lvd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF173639 mRNA. Translation: AAG45672.1 .
    BC007480 mRNA. Translation: AAH07480.1 .
    CCDSi CCDS16615.1.
    RefSeqi NP_598599.2. NM_133838.4.
    UniGenei Mm.132226.

    3D structure databases

    ProteinModelPortali Q9EQP2.
    SMRi Q9EQP2. Positions 1-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 221151. 1 interaction.
    IntActi Q9EQP2. 3 interactions.
    MINTi MINT-4094173.

    PTM databases

    PhosphoSitei Q9EQP2.

    Proteomic databases

    MaxQBi Q9EQP2.
    PaxDbi Q9EQP2.
    PRIDEi Q9EQP2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028755 ; ENSMUSP00000028755 ; ENSMUSG00000027293 .
    GeneIDi 98878.
    KEGGi mmu:98878.
    UCSCi uc008lvd.1. mouse.

    Organism-specific databases

    CTDi 30844.
    MGIi MGI:1919619. Ehd4.

    Phylogenomic databases

    eggNOGi NOG136252.
    GeneTreei ENSGT00700000104190.
    HOVERGENi HBG018183.
    InParanoidi Q9EQP2.
    KOi K12477.
    OMAi MPNQVLQ.
    OrthoDBi EOG757CX9.
    PhylomeDBi Q9EQP2.
    TreeFami TF314429.

    Miscellaneous databases

    ChiTaRSi EHD4. mouse.
    NextBioi 353697.
    PROi Q9EQP2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9EQP2.
    Bgeei Q9EQP2.
    CleanExi MM_EHD4.
    Genevestigatori Q9EQP2.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    3.40.50.300. 3 hits.
    InterProi IPR001401. Dynamin_GTPase.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00350. Dynamin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00027. EH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50031. EH. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MPAST2, a novel murine isoform of the PAST protein family."
      Plomann M., Behrendt D., Ritter B., Modregger J., Halbach A., Paulsson M.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    3. "EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling."
      Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B.
      Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion."
      Posey A.D. Jr., Pytel P., Gardikiotes K., Demonbreun A.R., Rainey M., George M., Band H., McNally E.M.
      J. Biol. Chem. 286:7379-7388(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiEHD4_MOUSE
    AccessioniPrimary (citable) accession number: Q9EQP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3