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Q9EQP2

- EHD4_MOUSE

UniProt

Q9EQP2 - EHD4_MOUSE

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Protein

EH domain-containing protein 4

Gene

Ehd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in early endosomal transport.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231ATPBy similarity
Binding sitei261 – 2611ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 758ATPBy similarity
Calcium bindingi492 – 50312PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. GTPase activity Source: InterPro
  4. GTP binding Source: InterPro

GO - Biological processi

  1. cellular response to growth factor stimulus Source: Ensembl
  2. endocytic recycling Source: UniProtKB
  3. pinocytosis Source: Ensembl
  4. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  5. protein homooligomerization Source: UniProtKB
  6. regulation of endocytosis Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 4
Alternative name(s):
PAST homolog 2
Short name:
mPAST2
Gene namesi
Name:Ehd4
Synonyms:Past2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1919619. Ehd4.

Subcellular locationi

Cell membrane 1 Publication. Early endosome membrane By similarity; Peripheral membrane protein By similarity. Recycling endosome membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
  3. perinuclear region of cytoplasm Source: MGI
  4. plasma membrane Source: UniProtKB-KW
  5. recycling endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 541541EH domain-containing protein 4PRO_0000146115Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei451 – 4511PhosphotyrosineBy similarity
Modified residuei459 – 4591PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9EQP2.
PaxDbiQ9EQP2.
PRIDEiQ9EQP2.

PTM databases

PhosphoSiteiQ9EQP2.

Expressioni

Gene expression databases

BgeeiQ9EQP2.
CleanExiMM_EHD4.
ExpressionAtlasiQ9EQP2. baseline and differential.
GenevestigatoriQ9EQP2.

Interactioni

Subunit structurei

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Pacsin2Q9QY174EBI-491022,EBI-491201From a different organism.
Snap29Q9Z2P62EBI-491022,EBI-492883From a different organism.

Protein-protein interaction databases

BioGridi221151. 1 interaction.
IntActiQ9EQP2. 3 interactions.
MINTiMINT-4094173.

Structurei

3D structure databases

ProteinModelPortaliQ9EQP2.
SMRiQ9EQP2. Positions 1-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 289232Dynamin-type GAdd
BLAST
Domaini447 – 53589EHPROSITE-ProRule annotationAdd
BLAST
Domaini479 – 51436EF-handPROSITE-ProRule annotationAdd
BLAST

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

Sequence similaritiesi

Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 EH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG136252.
GeneTreeiENSGT00760000118985.
HOVERGENiHBG018183.
InParanoidiQ9EQP2.
KOiK12477.
OMAiMPNQVLQ.
OrthoDBiEOG757CX9.
PhylomeDBiQ9EQP2.
TreeFamiTF314429.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQP2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS
60 70 80 90 100
PALEDADFEN KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF
110 120 130 140 150
IAVMYGETEG STPGNALVVD PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK
160 170 180 190 200
SISIIDSPGI LSGEKQRISR GYDFCQVLQW FAERVDRIIL LFDAHKLDIS
210 220 230 240 250
DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM WSLGKVINTP
260 270 280 290 300
EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL
310 320 330 340 350
IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ
360 370 380 390 400
ISAGDFPEVK AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI
410 420 430 440 450
SQEEMNMPTQ MVQGGAFDGT TEGPFNQGYG EGAKEGADEE EWVVAKDKPV
460 470 480 490 500
YDELFYTLSP INGKISGVNA KKEMVTSKLP NSVLGKIWKL ADCDCDGMLD
510 520 530 540
EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA D
Length:541
Mass (Da):61,481
Last modified:March 1, 2001 - v1
Checksum:iD34C28F046D740E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173639 mRNA. Translation: AAG45672.1.
BC007480 mRNA. Translation: AAH07480.1.
CCDSiCCDS16615.1.
RefSeqiNP_598599.2. NM_133838.4.
UniGeneiMm.132226.

Genome annotation databases

EnsembliENSMUST00000028755; ENSMUSP00000028755; ENSMUSG00000027293.
GeneIDi98878.
KEGGimmu:98878.
UCSCiuc008lvd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173639 mRNA. Translation: AAG45672.1 .
BC007480 mRNA. Translation: AAH07480.1 .
CCDSi CCDS16615.1.
RefSeqi NP_598599.2. NM_133838.4.
UniGenei Mm.132226.

3D structure databases

ProteinModelPortali Q9EQP2.
SMRi Q9EQP2. Positions 1-535.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 221151. 1 interaction.
IntActi Q9EQP2. 3 interactions.
MINTi MINT-4094173.

PTM databases

PhosphoSitei Q9EQP2.

Proteomic databases

MaxQBi Q9EQP2.
PaxDbi Q9EQP2.
PRIDEi Q9EQP2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028755 ; ENSMUSP00000028755 ; ENSMUSG00000027293 .
GeneIDi 98878.
KEGGi mmu:98878.
UCSCi uc008lvd.1. mouse.

Organism-specific databases

CTDi 30844.
MGIi MGI:1919619. Ehd4.

Phylogenomic databases

eggNOGi NOG136252.
GeneTreei ENSGT00760000118985.
HOVERGENi HBG018183.
InParanoidi Q9EQP2.
KOi K12477.
OMAi MPNQVLQ.
OrthoDBi EOG757CX9.
PhylomeDBi Q9EQP2.
TreeFami TF314429.

Miscellaneous databases

NextBioi 353697.
PROi Q9EQP2.
SOURCEi Search...

Gene expression databases

Bgeei Q9EQP2.
CleanExi MM_EHD4.
ExpressionAtlasi Q9EQP2. baseline and differential.
Genevestigatori Q9EQP2.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
3.40.50.300. 3 hits.
InterProi IPR001401. Dynamin_GTPase.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00350. Dynamin_N. 1 hit.
[Graphical view ]
SMARTi SM00027. EH. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MPAST2, a novel murine isoform of the PAST protein family."
    Plomann M., Behrendt D., Ritter B., Modregger J., Halbach A., Paulsson M.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  3. "EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling."
    Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B.
    Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion."
    Posey A.D. Jr., Pytel P., Gardikiotes K., Demonbreun A.R., Rainey M., George M., Band H., McNally E.M.
    J. Biol. Chem. 286:7379-7388(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiEHD4_MOUSE
AccessioniPrimary (citable) accession number: Q9EQP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3