Q9EQP2 (EHD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EH domain-containing protein 4

Alternative name(s):
PAST homolog 2
Short name=mPAST2

Gene names

Name:	Ehd4
Synonyms:	Past2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in early endosomal transport. Ref.3
Subunit structure	Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3 By similarity.
Subcellular location	Early endosome membrane; Peripheral membrane protein By similarity. Recycling endosome membrane; Peripheral membrane protein By similarity.
Domain	The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins By similarity.
Sequence similarities	Contains 1 EF-hand domain. Contains 1 EH domain.

Ontologies

Keywords
Cellular component	Endosome Membrane
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara endocytic recycling Inferred from sequence or structural similarity. Source: UniProtKB pinocytosis Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB regulation of endocytosis Inferred from electronic annotation. Source: Compara
Cellular_component	early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from direct assay PubMed 20489164. Source: MGI plasma membrane Inferred from electronic annotation. Source: Compara recycling endosome membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: InterPro GTPase activity Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pacsin2	Q9QY17	4	EBI-491022,EBI-491201	From a different organism.
Snap29	Q9Z2P6	2	EBI-491022,EBI-492883	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	EH domain-containing protein 4		PRO_0000146115
Regions
	Domain	447 – 535	89	EH
	Domain	479 – 514	36	EF-hand
	Nucleotide binding	68 – 75	8	ATP By similarity
	Calcium binding	492 – 503	12	By similarity
Sites
	Binding site	223	1	ATP By similarity
	Binding site	261	1	ATP By similarity
Amino acid modifications
	Modified residue	451	1	Phosphotyrosine By similarity
	Modified residue	459	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9EQP2 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D34C28F046D740E1	FASTA	541	61,481
	10 20 30 40 50 60 MFSWMGRQAG GRERSGGMDA VQTVTGGLRS LYQRKVLPLE EAYRFHEFHS PALEDADFEN 70 80 90 100 110 120 KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF IAVMYGETEG STPGNALVVD 130 140 150 160 170 180 PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK SISIIDSPGI LSGEKQRISR GYDFCQVLQW 190 200 210 220 230 240 FAERVDRIIL LFDAHKLDIS DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM 250 260 270 280 290 300 WSLGKVINTP EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL 310 320 330 340 350 360 IKRARLAKVH AYIISYLKKE MPNMFGKENK KRELIYRLPE IYVQLQREYQ ISAGDFPEVK 370 380 390 400 410 420 AMQEQLENYD FTKFHSLKPK LIEAVDNMLT NKISSLMGLI SQEEMNMPTQ MVQGGAFDGT 430 440 450 460 470 480 TEGPFNQGYG EGAKEGADEE EWVVAKDKPV YDELFYTLSP INGKISGVNA KKEMVTSKLP 490 500 510 520 530 540 NSVLGKIWKL ADCDCDGMLD EEEFALAKHL IKIKLDGYEL PNSLPPHLVP PSHRKSLPKA D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MPAST2, a novel murine isoform of the PAST protein family." Plomann M., Behrendt D., Ritter B., Modregger J., Halbach A., Paulsson M. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[3]	"EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling." Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B. Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-459, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF173639 mRNA. Translation: AAG45672.1. BC007480 mRNA. Translation: AAH07480.1.
IPI	IPI00318671.
RefSeq	NP_598599.2. NM_133838.4.
UniGene	Mm.132226.
3D structure databases
ProteinModelPortal	Q9EQP2.
SMR	Q9EQP2. Positions 22-535.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9EQP2. 2 interactions.
PTM databases
PhosphoSite	Q9EQP2.
Proteomic databases
PaxDb	Q9EQP2.
PRIDE	Q9EQP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028755; ENSMUSP00000028755; ENSMUSG00000027293.
GeneID	98878.
KEGG	mmu:98878.
Organism-specific databases
CTD	30844.
MGI	MGI:1919619. Ehd4.
Phylogenomic databases
eggNOG	NOG136252.
GeneTree	ENSGT00700000104190.
HOVERGEN	HBG018183.
InParanoid	Q9EQP2.
KO	K12477.
OMA	TENRRLF.
OrthoDB	EOG418BN4.
Gene expression databases
ArrayExpress	Q9EQP2.
Bgee	Q9EQP2.
CleanEx	MM_EHD4.
Genevestigator	Q9EQP2.
GermOnline	ENSMUSG00000027293. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR001401. Dynamin_GTPase. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR000261. EPS15_homology. [Graphical view]
Pfam	PF00350. Dynamin_N. 1 hit. [Graphical view]
SMART	SM00027. EH. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS50031. EH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EHD4. mouse.
NextBio	353697.
SOURCE	Search...

Entry information

Entry name

EHD4_MOUSE

Accession

Primary (citable) accession number: Q9EQP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program