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Protein

DNA-binding protein SMUBP-2

Gene

Ighmbp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

5' to 3' helicase that unwinds RNA and DNA duplices in an ATP-dependent reaction. Acts as a transcription regulator. Required for the transcriptional activation of the flounder liver-type antifreeze protein gene. Exhibits strong binding specificity to the enhancer element B of the flounder antifreeze protein gene intron. Binds to the insulin II gene RIPE3B enhancer region (By similarity). May be involved in translation. DNA-binding protein specific to 5'-phosphorylated single-stranded guanine-rich sequence related to the immunoglobulin mu chain switch region. Preferentially binds to the 5'-GGGCT-3' motif. Interacts with tRNA-Tyr (By similarity).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi213 – 2208ATPBy similarity
Zinc fingeri887 – 93347AN1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Helicase, Hydrolase, Ribonucleoprotein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein SMUBP-2 (EC:3.6.4.12, EC:3.6.4.13)
Alternative name(s):
ATP-dependent helicase IGHMBP2
Antifreeze enhancer-binding protein ortholog
Short name:
AEP
Gene namesi
Name:Ighmbp2
Synonyms:Smbp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68325. Ighmbp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 988987DNA-binding protein SMUBP-2PRO_0000372431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei797 – 7971PhosphoserineBy similarity
Modified residuei800 – 8001PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9EQN5.
PRIDEiQ9EQN5.

Expressioni

Tissue specificityi

Expressed in liver, skin, muscle, heart, brain, spleen and kidney.1 Publication

Interactioni

Subunit structurei

Homooligomer. Interacts with RUVBL1, RUVBL2, GTF3C1 and ABT1. Is part of large cytosolic ribonucleoprotein complexes. Associates with the ribosomes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018487.

Structurei

3D structure databases

ProteinModelPortaliQ9EQN5.
SMRiQ9EQN5. Positions 725-784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini721 – 78464R3HPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni637 – 783147SS DNA-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili957 – 98630Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi857 – 8615Nuclear localization signalSequence analysis

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated
Contains 1 AN1-type zinc finger.PROSITE-ProRule annotation
Contains 1 R3H domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri887 – 93347AN1-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1803. Eukaryota.
COG1112. LUCA.
HOGENOMiHOG000185831.
InParanoidiQ9EQN5.
KOiK19036.
PhylomeDBiQ9EQN5.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.40.50.300. 3 hits.
4.10.1110.10. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR014001. Helicase_ATP-bd.
IPR033098. IGHMBP2.
IPR027417. P-loop_NTPase.
IPR001374. R3H_dom.
IPR004483. SMUBP-2/Hcs1-like.
IPR000058. Znf_AN1.
[Graphical view]
PANTHERiPTHR10887:SF375. PTHR10887:SF375. 1 hit.
PfamiPF01424. R3H. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00393. R3H. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF82708. SSF82708. 1 hit.
TIGRFAMsiTIGR00376. TIGR00376. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EQN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASYTVESFV AQQLQLLELE RDAEVEERRS WQEHSSLKEL QSRGVCLLKL
60 70 80 90 100
QVSGQRTGLY GQRLVTFEPR KFGPAVVLPS NSFTSGDIVG LYDTNESSQL
110 120 130 140 150
ATGVLTRITQ KSVIVAFDES HDFQLNLDRE NTYRLLKLAN DVTYKRLKKA
160 170 180 190 200
LLTLKKYHSG PASSLIDVLL GGSTPSPATE IPPLTFYNTT LDPSQKEAVS
210 220 230 240 250
FALAQKEVAI IHGPPGTGKT TTVVEIILQA VKQGLKVLCC APSNIAVDNL
260 270 280 290 300
VERLALCKKQ ILRLGHPARL LESVQQHSLD AVLARSDNAQ IVADIRRDID
310 320 330 340 350
QVFGKNKKTQ DKREKSNFRN EIKLLRKELK EREEAAIVQS LSAADVVLAT
360 370 380 390 400
NTGASTDGPL KLLPEDYFDV VVVDECAQAL EASCWIPLLK APKCILAGDH
410 420 430 440 450
KQLPPTTVSH KAALAGLSRS LMERLAEKHG AAVVRMLAVQ YRMHQAITRW
460 470 480 490 500
ASEAMYHGQL TAHPSVAGHL LKDLPGVADT EETSVPLLLI DTAGCGLLEL
510 520 530 540 550
EEEDSQSKGN PGEVRLVTLH IQALVDAGVQ AGDIAVIAPY NLQVDLLRQS
560 570 580 590 600
LSNKHPELEI KSVDGFQGRE KEAVILTFVR SNRKGEVGFL AEDRRINVAV
610 620 630 640 650
TRARRHVAVI CDSHTVNNHA FLKTLVDYFT EHGEVRTAFE YLDDIVPENY
660 670 680 690 700
THEGSRSHSC APKPKCPTTS VRKPASAQES RQEARAATGH SRRKPSEKPL
710 720 730 740 750
GSQVQPQHSS KANGSDRTGG TDRTEHFRAM IEEFVASKEA QLEFPTSLSS
760 770 780 790 800
HDRLRVHQLA EEFGLKHDST GEGKARHITV SRRSPAGSGS ATPQPPSPPS
810 820 830 840 850
PAQAEPEPQV EQPVGQPHGS TQLDLKALHL ERLQRQQGCQ AQSQLGGGSR
860 870 880 890 900
PQKAPQKKKK KEPKGPAMAL PSEEDFDALV SAVVKADNTC SFTKCSASTT
910 920 930 940 950
TLGQFCMHCS RRYCLSHHLP EIHGCGEKAR AHARQRISRE GVLYAGSGTK
960 970 980
DRALDPAKRA QLQRKLDKKL GELSSQRTSK KKEKERGT
Length:988
Mass (Da):108,440
Last modified:March 1, 2001 - v1
Checksum:i624ED6122F8C8D8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199411 mRNA. Translation: AAG28561.1.
RefSeqiNP_113774.1. NM_031586.1.
UniGeneiRn.23984.

Genome annotation databases

GeneIDi29532.
KEGGirno:29532.
UCSCiRGD:68325. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199411 mRNA. Translation: AAG28561.1.
RefSeqiNP_113774.1. NM_031586.1.
UniGeneiRn.23984.

3D structure databases

ProteinModelPortaliQ9EQN5.
SMRiQ9EQN5. Positions 725-784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018487.

Proteomic databases

PaxDbiQ9EQN5.
PRIDEiQ9EQN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29532.
KEGGirno:29532.
UCSCiRGD:68325. rat.

Organism-specific databases

CTDi3508.
RGDi68325. Ighmbp2.

Phylogenomic databases

eggNOGiKOG1803. Eukaryota.
COG1112. LUCA.
HOGENOMiHOG000185831.
InParanoidiQ9EQN5.
KOiK19036.
PhylomeDBiQ9EQN5.

Miscellaneous databases

PROiQ9EQN5.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.40.50.300. 3 hits.
4.10.1110.10. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR014001. Helicase_ATP-bd.
IPR033098. IGHMBP2.
IPR027417. P-loop_NTPase.
IPR001374. R3H_dom.
IPR004483. SMUBP-2/Hcs1-like.
IPR000058. Znf_AN1.
[Graphical view]
PANTHERiPTHR10887:SF375. PTHR10887:SF375. 1 hit.
PfamiPF01424. R3H. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00393. R3H. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF82708. SSF82708. 1 hit.
TIGRFAMsiTIGR00376. TIGR00376. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The rat ortholog of the presumptive flounder antifreeze enhancer-binding protein is a helicase domain-containing protein."
    Miao M., Chan S.-L., Fletcher G.L., Hew C.L.
    Eur. J. Biochem. 267:7237-7246(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DNA-BINDING, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiSMBP2_RAT
AccessioniPrimary (citable) accession number: Q9EQN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.