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Protein

TSC22 domain family protein 4

Gene

Tsc22d4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor.By similarity

GO - Molecular functioni

GO - Biological processi

  • response to osmotic stress Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
TSC22 domain family protein 4
Alternative name(s):
TSC22-related-inducible leucine zipper protein 2
Thg-1pit
Gene namesi
Name:Tsc22d4
Synonyms:Thg1pit, Tilz2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1926079. Tsc22d4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387TSC22 domain family protein 4PRO_0000219375Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei165 – 1651PhosphoserineCombined sources
Modified residuei183 – 1831PhosphothreonineCombined sources
Modified residuei187 – 1871PhosphoserineCombined sources
Modified residuei189 – 1891PhosphoserineCombined sources
Modified residuei219 – 2191PhosphoserineCombined sources
Modified residuei223 – 2231PhosphothreonineCombined sources
Modified residuei254 – 2541PhosphoserineCombined sources
Modified residuei258 – 2581PhosphoserineCombined sources
Modified residuei271 – 2711PhosphoserineCombined sources
Modified residuei362 – 3621PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9EQN3.
MaxQBiQ9EQN3.
PaxDbiQ9EQN3.
PRIDEiQ9EQN3.

PTM databases

iPTMnetiQ9EQN3.
PhosphoSiteiQ9EQN3.

Expressioni

Developmental stagei

Expression starts at 8.5 dpc and undergoes a second peak of activation at 12.5 dpc. At 12.5 dpc, expression encompasses the entire central nervous system, with highest levels in the dorsal root and trigeminal ganglia.1 Publication

Inductioni

Up-regulated by TGF-beta treatment.1 Publication

Gene expression databases

BgeeiQ9EQN3.
CleanExiMM_TSC22D4.
ExpressionAtlasiQ9EQN3. baseline and differential.
GenevisibleiQ9EQN3. MM.

Interactioni

Subunit structurei

Forms a homodimer or heterodimer. Can form a heterodimer with TSC22D1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Aifm1Q9Z0X14EBI-7821198,EBI-773597
Tsc22d1P625002EBI-7821198,EBI-8296837

Protein-protein interaction databases

BioGridi219663. 1 interaction.
IntActiQ9EQN3. 3 interactions.
STRINGi10090.ENSMUSP00000098108.

Structurei

3D structure databases

ProteinModelPortaliQ9EQN3.
SMRiQ9EQN3. Positions 318-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni336 – 35722Leucine-zipperAdd
BLAST

Sequence similaritiesi

Belongs to the TSC-22/Dip/Bun family.Curated

Phylogenomic databases

eggNOGiKOG4797. Eukaryota.
ENOG411251N. LUCA.
GeneTreeiENSGT00530000063062.
HOGENOMiHOG000253006.
HOVERGENiHBG069048.
InParanoidiQ9EQN3.
OMAiCVDIYER.
TreeFamiTF338725.

Family and domain databases

InterProiIPR000580. TSC-22_Dip_Bun.
[Graphical view]
PANTHERiPTHR12348. PTHR12348. 1 hit.
PfamiPF01166. TSC22. 1 hit.
[Graphical view]
ProDomiPD007152. TSC-22_Dip_Bun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01289. TSC22. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGKKKSSF QITSVTTDYE GPGSPGASDS PVPPALAGPP PRLPNGDPNP
60 70 80 90 100
DPGGRGTPRN GSPPPGAPAS RFRVVKLPQG LGEPYRRGRW TCVDVYERDL
110 120 130 140 150
EPPSFGRLLE GIRGASGGTG GRSLDSRLEL ASLGISTPIP QPGLSQGPTS
160 170 180 190 200
WLRPPPTSPG PQARSFTGGL GQLAGPGKAK VETPPLSASP PQQRPPGPGT
210 220 230 240 250
GDSAQTLPSL RVEVESGGSA AATPPLSRRR DGAVRLRMEL VAPAETGKVP
260 270 280 290 300
PTDSRPNSPA LYFDASLVHK SPDPFGAAAA QSLSLARSML AISGHLDSDD
310 320 330 340 350
DSGSGSLVGI DNKIEQAMDL VKSHLMFAVR EEVEVLKEQI RDLAERNAAL
360 370 380
EQENGLLRAL ASPEQLAQLP SSGLPRLGPS APNGPSI
Length:387
Mass (Da):39,979
Last modified:July 27, 2011 - v2
Checksum:iD4160FA3AB2DFB90
GO

Sequence cautioni

The sequence AAK02018.1 differs from that shown. Reason: Frameshift at positions 222 and 251. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971E → K in AAK02018 (PubMed:11707329).Curated
Sequence conflicti339 – 3391Q → H in AAG41219 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF315352 mRNA. Translation: AAK02018.1. Frameshift.
AF201286 mRNA. Translation: AAG41219.1.
BC018544 mRNA. Translation: AAH18544.1.
BC023761 mRNA. Translation: AAH23761.1.
BC145721 mRNA. Translation: AAI45722.1.
BC145723 mRNA. Translation: AAI45724.1.
AK018735 mRNA. Translation: BAB31377.1.
AK143608 mRNA. Translation: BAE25460.1.
CH466529 Genomic DNA. Translation: EDL19240.1.
CCDSiCCDS19776.1.
RefSeqiNP_076399.4. NM_023910.6.
UniGeneiMm.286399.
Mm.486508.
Mm.491129.

Genome annotation databases

EnsembliENSMUST00000100539; ENSMUSP00000098107; ENSMUSG00000029723.
GeneIDi78829.
KEGGimmu:78829.
UCSCiuc009adu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF315352 mRNA. Translation: AAK02018.1. Frameshift.
AF201286 mRNA. Translation: AAG41219.1.
BC018544 mRNA. Translation: AAH18544.1.
BC023761 mRNA. Translation: AAH23761.1.
BC145721 mRNA. Translation: AAI45722.1.
BC145723 mRNA. Translation: AAI45724.1.
AK018735 mRNA. Translation: BAB31377.1.
AK143608 mRNA. Translation: BAE25460.1.
CH466529 Genomic DNA. Translation: EDL19240.1.
CCDSiCCDS19776.1.
RefSeqiNP_076399.4. NM_023910.6.
UniGeneiMm.286399.
Mm.486508.
Mm.491129.

3D structure databases

ProteinModelPortaliQ9EQN3.
SMRiQ9EQN3. Positions 318-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219663. 1 interaction.
IntActiQ9EQN3. 3 interactions.
STRINGi10090.ENSMUSP00000098108.

PTM databases

iPTMnetiQ9EQN3.
PhosphoSiteiQ9EQN3.

Proteomic databases

EPDiQ9EQN3.
MaxQBiQ9EQN3.
PaxDbiQ9EQN3.
PRIDEiQ9EQN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100539; ENSMUSP00000098107; ENSMUSG00000029723.
GeneIDi78829.
KEGGimmu:78829.
UCSCiuc009adu.2. mouse.

Organism-specific databases

CTDi81628.
MGIiMGI:1926079. Tsc22d4.

Phylogenomic databases

eggNOGiKOG4797. Eukaryota.
ENOG411251N. LUCA.
GeneTreeiENSGT00530000063062.
HOGENOMiHOG000253006.
HOVERGENiHBG069048.
InParanoidiQ9EQN3.
OMAiCVDIYER.
TreeFamiTF338725.

Miscellaneous databases

NextBioi349604.
PROiQ9EQN3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EQN3.
CleanExiMM_TSC22D4.
ExpressionAtlasiQ9EQN3. baseline and differential.
GenevisibleiQ9EQN3. MM.

Family and domain databases

InterProiIPR000580. TSC-22_Dip_Bun.
[Graphical view]
PANTHERiPTHR12348. PTHR12348. 1 hit.
PfamiPF01166. TSC22. 1 hit.
[Graphical view]
ProDomiPD007152. TSC-22_Dip_Bun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01289. TSC22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression screening for Lhx3 downstream genes identifies Thg-1pit as a novel mouse gene involved in pituitary development."
    Fiorenza M.T., Mukhopadhyay M., Westphal H.
    Gene 278:125-130(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, INDUCTION.
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. "Identification and characterization of a family of leucine zipper genes related to TSC22."
    Ershler M.A., Belyavsky A.V., Visser J.W.M.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney and Spleen.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; THR-183; SER-187; SER-189; SER-219; THR-223; SER-254; SER-258 AND SER-271, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiT22D4_MOUSE
AccessioniPrimary (citable) accession number: Q9EQN3
Secondary accession number(s): Q99PD5, Q9D2V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: March 16, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.