Reviewed, UniProtKB/Swiss-Prot Q9EQM3 (NKX24_MOUSE)

Names and origin

Protein names

Recommended name:
Homeobox protein Nkx-2.4

Alternative name(s):
Homeobox protein NK-2 homolog D

Gene names

Name:	Nkx2-4
Synonyms:	Nkx2d

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable transcription factor.
Subcellular location	Nucleus By similarity.
Tissue specificity	In the embryo it is detected in the posterior hypotalamus and later in the head. In the adult it is detected only in testis.
Developmental stage	Expressed in a restricted region of the posterior hypothalamus from E10. Detected in the head region from E12.5 to E14.5. Expression is down-regulated by E15.5.
Sequence similarities	Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of cellular transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Homeobox protein Nkx-2.4		PRO_0000048936
Regions
	DNA binding	188 – 247	60	Homeobox
	Compositional bias	76 – 91	16	Poly-Ala
	Compositional bias	132 – 136	5	Poly-Ala
	Compositional bias	263 – 272	10	Poly-Pro

Sequences

	Sequence		Length	Mass (Da)
	Q9EQM3-1 [UniParc]. Last modified January 27, 2003. Version 2. Checksum: A7A0F450DB15616C	FASTA	354	36,225
	10 20 30 40 50 60 MSLSPKHTTP FSVSDILSPI EETYKKFGGV MDGAPPGLGA PLGAAAYRAP PSGPSSQAAA 70 80 90 100 110 120 VAAGMQPPHA MAGHNAAAAA AAAAAAAAAA ATYHMPPGVS QFPHSAMGSY CNGGLGNMGE 130 140 150 160 170 180 LPAYTDGMRG GAAAAATGWY GANTDPRYSS ISRFMGPSAG VNVAGMGSLT GIADAAKSLA 190 200 210 220 230 240 PLHAAAPRRK RRVLFSQAQV YELERRFKQQ KYLSAPEREH LASMIHLTPT QVKIWFQNHR 250 260 270 280 290 300 YKMKRQAKDK AAQQLQQEGG LGPPPPPPPP SPRRVAVPVL VKDGKPCQNG AGTPTPGQGG 310 320 330 340 350 QQPQAPTPAP ELEELSPSPP ALHGPGGGLA ALDAATGDYG GGVLGANLLY GRTW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conserved linkage of NK-2 homeobox gene pairs Nkx2-2/2-4 and Nkx2-1/2-9 in mammals." Wang C.-C., Brodnicki T., Copeland N.G., Jenkins N.A., Harvey R.P. Mamm. Genome 11:466-468(2000) [PubMed: 10818213] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/Sv and C57BL/6.
[2]	"Regional expression of the homeobox gene Nkx-2.2 in the developing mammalian forebrain." Price M., Lazzaro D., Pohl T., Mattei M.-G., Ruether U., Olivo J.-C., Duboule D., di Lauro R. Neuron 8:241-255(1992) [PubMed: 1346742] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE. Strain: C57BL/6.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF202038 Genomic DNA. Translation: AAG35618.1. AF202039 mRNA. Translation: AAG35619.1. AL928876 Genomic DNA. Translation: CAM18290.1.
IPI	IPI00111298.
RefSeq	NP_075993.1.
UniGene	Mm.315498
3D structure databases
SMR	Q9EQM3. Positions 139-246, 188-254.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9EQM3.
Proteomic databases
PRIDE	Q9EQM3.
Genome annotation databases
Ensembl	ENSMUST00000067020; ENSMUSP00000066690; ENSMUSG00000054160; Mus musculus. [Genome view]
GeneID	228731.
KEGG	mmu:228731.
UCSC	uc008mss.1. mouse.
Organism-specific databases
CTD	228731.
MGI	MGI:97349. Nkx2-4.
Phylogenomic databases
HOGENOM	HBG715804.
HOVERGEN	HBG006689.
InParanoid	Q9EQM3.
OMA	YCNGGLG.
PhylomeDB	Q9EQM3.
Gene expression databases
ArrayExpress	Q9EQM3.
Bgee	Q9EQM3.
Genevestigator	Q9EQM3.
GermOnline	ENSMUSG00000054160. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_region. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	379096.
SOURCE	Search...

Entry information

Entry name

NKX24_MOUSE

Accession

Primary (citable) accession number: Q9EQM3
Secondary accession number(s): A2ATK2, Q9EQM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	January 27, 2003
Last modified:	March 2, 2010
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)