Reviewed, UniProtKB/Swiss-Prot Q9EQL9 (SHRPN_RAT)

Names and origin

Protein names

Recommended name:
    Sharpin
Alternative name(s):
    Shank-associated RH domain-interacting protein

Gene names

Name:

Sharpin

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a role in normal immune development and control of inflammation By similarity.
Subunit structure	Self-associates. Interacts with SHANK1. Ref.1
Subcellular location	Cytoplasm. Note: Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. Ref.1
Tissue specificity	Expressed in brain, spleen, lung, heart, skeletal muscle, kidney and testis (at protein level). Expressed in heart and testis. Ref.1
Sequence similarities	Contains 1 RanBP2-type zinc finger.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Ref.1 Traceable author statement. Source: RGD
Molecular function	identical protein binding Ref.1 Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-1394695,EBI-1394695
Dlg4	P31016	1	EBI-1394695,EBI-375655
Grip1	P97879	1	EBI-1394695,EBI-936113
Shank1	Q9WV48	4	EBI-1394695,EBI-80909

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Sharpin		PRO_0000280636
Regions
	Zinc finger	342 – 371	30	RanBP2-type
	Region	1 – 177	177	Self-association
	Region	172 – 305	134	Interaction with SHANK1

Sequences

	Sequence		Length	Mass (Da)
	Q9EQL9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 8D9A7D7D23665567	FASTA	381	40,287
	10 20 30 40 50 60 MSPPAGGAAA AADPASPVVL LAVQAAVRLL GAGHEDEAQL RKLQLKADPE RPGRFRLGLL 70 80 90 100 110 120 GIEPGAVSLE WPLESICYTI RGPNQHELQP PPGGPGTFSV HFLNSEEAQQ WAALVRDATA 130 140 150 160 170 180 EGQNGNDSTA PVPTPAMCPT SPPCSSVTPT PKATQPEMDL PQGSGNLKKE ELATHLAQAI 190 200 210 220 230 240 AGGDEKAAAQ VAAILAQHHV ALNVQLLEAW FPRGPIRLQV TVEDATSVLS SSSSAHVSLQ 250 260 270 280 290 300 IHPHCSIAAL QEQVFSEFGF PPAVQRWVIG RCLCMPERSL ASYGVSQDGD PAFLYLLSAP 310 320 330 340 350 360 REVSGHSPQH SKMDRKLGCL FPQSLELPHN LQASSSSLPS PPQPGWSCPS CTFINASNRP 370 380 GCEMCSTQRP CAWDPLTATS T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins." Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Mol. Cell. Neurosci. 17:385-397(2001) [PubMed: 11178875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SELF-ASSOCIATION, INTERACTION WITH SHANK1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF203906 mRNA. Translation: AAG43482.1. BC083553 mRNA. Translation: AAH83553.1.
IPI	IPI00190132.
RefSeq	NP_112415.1.
UniGene	Rn.38393
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9EQL9. 4 interactions.
Genome annotation databases
Ensembl	ENSRNOG00000012812. Rattus norvegicus. [Contig view]
GeneID	81859.
KEGG	rno:81859.
Organism-specific databases
RGD	631353. Sharpin.
Phylogenomic databases
HOVERGEN	Q9EQL9.
OMA	Q9EQL9. PERPGRF.
Gene expression databases
ArrayExpress	Q9EQL9.
Family and domain databases
InterPro	IPR001876. Znf_RanBP2. [Graphical view]
SMART	SM00547. ZnF_RBZ. 1 hit. [Graphical view]
PROSITE	PS01358. ZF_RANBP2_1. 1 hit. PS50199. ZF_RANBP2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615783.

Entry information

Entry name

SHRPN_RAT

Accession

Primary (citable) accession number: Q9EQL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)