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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

Icmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Divalent metal cations. Probably Zn2+.By similarity

GO - Molecular functioni

  • cAMP response element binding protein binding Source: MGI
  • protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: MGI

GO - Biological processi

  • C-terminal protein methylation Source: MGI
  • in utero embryonic development Source: MGI
  • liver development Source: MGI
  • multicellular organism growth Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • protein localization Source: MGI
  • protein methylation Source: MGI
  • regulation of Ras protein signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ9EQK7.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:Icmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1888594. Icmt.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 32HelicalSequence analysisAdd BLAST17
Topological domaini33 – 40LumenalSequence analysis8
Transmembranei41 – 58HelicalSequence analysisAdd BLAST18
Topological domaini59 – 68CytoplasmicSequence analysis10
Transmembranei69 – 86HelicalSequence analysisAdd BLAST18
Topological domaini87 – 91LumenalSequence analysis5
Transmembranei92 – 111HelicalSequence analysisAdd BLAST20
Topological domaini112 – 130CytoplasmicSequence analysisAdd BLAST19
Transmembranei131 – 148HelicalSequence analysisAdd BLAST18
Topological domaini149 – 153LumenalSequence analysis5
Transmembranei154 – 173HelicalSequence analysisAdd BLAST20
Topological domaini174 – 211CytoplasmicSequence analysisAdd BLAST38
Transmembranei212 – 227HelicalSequence analysisAdd BLAST16
Topological domaini228LumenalSequence analysis1
Transmembranei229 – 243HelicalSequence analysisAdd BLAST15
Topological domaini244 – 283CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002098951 – 283Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST283

Proteomic databases

MaxQBiQ9EQK7.
PaxDbiQ9EQK7.
PRIDEiQ9EQK7.

PTM databases

iPTMnetiQ9EQK7.
PhosphoSitePlusiQ9EQK7.

Expressioni

Tissue specificityi

Highly enriched in adult cerebellum, with a low level expression in other brain regions.

Developmental stagei

During cerebellar development expression was low before postnatal day P6 and dramatically increased after P12.

Gene expression databases

CleanExiMM_ICMT.

Interactioni

GO - Molecular functioni

  • cAMP response element binding protein binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043390.

Structurei

3D structure databases

ProteinModelPortaliQ9EQK7.
SMRiQ9EQK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2628. Eukaryota.
COG2020. LUCA.
HOVERGENiHBG019034.
InParanoidiQ9EQK7.
KOiK00587.
PhylomeDBiQ9EQK7.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAARRGSAG SEARLSLATF LLGASVLALP LLTRAGLQGR TGLALYVAGL
60 70 80 90 100
NALLLLLYRP PRYQIAIRAC FLGFVFGCGV LLSFSQSSWN HFGWYVCSLS
110 120 130 140 150
LFHYSEYLVT AVNNPKSLSL DSFLLNHSLE YTVAALSSWI EFTLENIFWP
160 170 180 190 200
ELKQITWLSA TGLLMVVFGE CLRKAAMFTA GSNFNHVVQS EKSDTHTLVT
210 220 230 240 250
SGVYAWCRHP SYVGWFYWSI GTQVMLCNPI CGVVYALTVW RFFRDRTEEE
260 270 280
EISLIHFFGE EYLDYKKRVP TGLPFIKGVK VEL
Length:283
Mass (Da):31,790
Last modified:June 20, 2001 - v2
Checksum:iA6C17436EDC27F40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24A → D in AA022288 (Ref. 2) Curated1
Sequence conflicti42G → A (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF209926 mRNA. Translation: AAG48552.1.
AA022288 mRNA. No translation available.
RefSeqiNP_598549.1. NM_133788.2.
UniGeneiMm.277464.

Genome annotation databases

GeneIDi57295.
KEGGimmu:57295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF209926 mRNA. Translation: AAG48552.1.
AA022288 mRNA. No translation available.
RefSeqiNP_598549.1. NM_133788.2.
UniGeneiMm.277464.

3D structure databases

ProteinModelPortaliQ9EQK7.
SMRiQ9EQK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043390.

PTM databases

iPTMnetiQ9EQK7.
PhosphoSitePlusiQ9EQK7.

Proteomic databases

MaxQBiQ9EQK7.
PaxDbiQ9EQK7.
PRIDEiQ9EQK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi57295.
KEGGimmu:57295.

Organism-specific databases

CTDi23463.
MGIiMGI:1888594. Icmt.

Phylogenomic databases

eggNOGiKOG2628. Eukaryota.
COG2020. LUCA.
HOVERGENiHBG019034.
InParanoidiQ9EQK7.
KOiK00587.
PhylomeDBiQ9EQK7.

Enzyme and pathway databases

SABIO-RKQ9EQK7.

Miscellaneous databases

PROiQ9EQK7.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ICMT.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICMT_MOUSE
AccessioniPrimary (citable) accession number: Q9EQK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.