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Q9EQK7

- ICMT_MOUSE

UniProt

Q9EQK7 - ICMT_MOUSE

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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

Icmt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.PROSITE-ProRule annotation

Cofactori

Divalent cations. Probably zinc By similarity.By similarity

GO - Molecular functioni

  1. cAMP response element binding protein binding Source: MGI
  2. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: MGI

GO - Biological processi

  1. C-terminal protein methylation Source: MGI
  2. in utero embryonic development Source: MGI
  3. liver development Source: MGI
  4. multicellular organism growth Source: MGI
  5. positive regulation of cell proliferation Source: MGI
  6. protein localization Source: MGI
  7. protein methylation Source: MGI
  8. regulation of Ras protein signal transduction Source: MGI
  9. regulation of RNA biosynthetic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKQ9EQK7.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:Icmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1888594. Icmt.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Protein-S-isoprenylcysteine O-methyltransferasePRO_0000209895Add
BLAST

Proteomic databases

MaxQBiQ9EQK7.
PaxDbiQ9EQK7.
PRIDEiQ9EQK7.

PTM databases

PhosphoSiteiQ9EQK7.

Expressioni

Tissue specificityi

Highly enriched in adult cerebellum, with a low level expression in other brain regions.

Developmental stagei

During cerebellar development expression was low before postnatal day P6 and dramatically increased after P12.

Gene expression databases

CleanExiMM_ICMT.
GenevestigatoriQ9EQK7.

Structurei

3D structure databases

ProteinModelPortaliQ9EQK7.
SMRiQ9EQK7. Positions 181-274.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini33 – 408LumenalSequence Analysis
Topological domaini59 – 6810CytoplasmicSequence Analysis
Topological domaini87 – 915LumenalSequence Analysis
Topological domaini112 – 13019CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini149 – 1535LumenalSequence Analysis
Topological domaini174 – 21138CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini228 – 2281LumenalSequence Analysis
Topological domaini244 – 28340CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3217HelicalSequence AnalysisAdd
BLAST
Transmembranei41 – 5818HelicalSequence AnalysisAdd
BLAST
Transmembranei69 – 8618HelicalSequence AnalysisAdd
BLAST
Transmembranei92 – 11120HelicalSequence AnalysisAdd
BLAST
Transmembranei131 – 14818HelicalSequence AnalysisAdd
BLAST
Transmembranei154 – 17320HelicalSequence AnalysisAdd
BLAST
Transmembranei212 – 22716HelicalSequence AnalysisAdd
BLAST
Transmembranei229 – 24315HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2020.
HOVERGENiHBG019034.
InParanoidiQ9EQK7.
KOiK00587.
PhylomeDBiQ9EQK7.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQK7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAARRGSAG SEARLSLATF LLGASVLALP LLTRAGLQGR TGLALYVAGL
60 70 80 90 100
NALLLLLYRP PRYQIAIRAC FLGFVFGCGV LLSFSQSSWN HFGWYVCSLS
110 120 130 140 150
LFHYSEYLVT AVNNPKSLSL DSFLLNHSLE YTVAALSSWI EFTLENIFWP
160 170 180 190 200
ELKQITWLSA TGLLMVVFGE CLRKAAMFTA GSNFNHVVQS EKSDTHTLVT
210 220 230 240 250
SGVYAWCRHP SYVGWFYWSI GTQVMLCNPI CGVVYALTVW RFFRDRTEEE
260 270 280
EISLIHFFGE EYLDYKKRVP TGLPFIKGVK VEL
Length:283
Mass (Da):31,790
Last modified:June 20, 2001 - v2
Checksum:iA6C17436EDC27F40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241A → D in AA022288. 1 PublicationCurated
Sequence conflicti42 – 421G → A1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF209926 mRNA. Translation: AAG48552.1.
AA022288 mRNA. No translation available.
RefSeqiNP_598549.1. NM_133788.2.
UniGeneiMm.277464.

Genome annotation databases

GeneIDi57295.
KEGGimmu:57295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF209926 mRNA. Translation: AAG48552.1 .
AA022288 mRNA. No translation available.
RefSeqi NP_598549.1. NM_133788.2.
UniGenei Mm.277464.

3D structure databases

ProteinModelPortali Q9EQK7.
SMRi Q9EQK7. Positions 181-274.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9EQK7.

Proteomic databases

MaxQBi Q9EQK7.
PaxDbi Q9EQK7.
PRIDEi Q9EQK7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 57295.
KEGGi mmu:57295.

Organism-specific databases

CTDi 23463.
MGIi MGI:1888594. Icmt.

Phylogenomic databases

eggNOGi COG2020.
HOVERGENi HBG019034.
InParanoidi Q9EQK7.
KOi K00587.
PhylomeDBi Q9EQK7.

Enzyme and pathway databases

SABIO-RK Q9EQK7.

Miscellaneous databases

NextBioi 313651.
PROi Q9EQK7.
SOURCEi Search...

Gene expression databases

CleanExi MM_ICMT.
Genevestigatori Q9EQK7.

Family and domain databases

InterProi IPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view ]
Pfami PF04140. ICMT. 1 hit.
[Graphical view ]
PROSITEi PS51564. SAM_ICMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Polyglutamine expansion down-regulates specific neuronal genes before pathologic changes in SCA1."
    Lin X., Antalffy B., Kang D., Orr H.T., Zoghbi H.Y.
    Nat. Neurosci. 3:157-163(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-283.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-153.
    Strain: C57BL/6J.
    Tissue: Placenta.

Entry informationi

Entry nameiICMT_MOUSE
AccessioniPrimary (citable) accession number: Q9EQK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3