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Q9EQK7

- ICMT_MOUSE

UniProt

Q9EQK7 - ICMT_MOUSE

Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

Icmt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (20 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.PROSITE-ProRule annotation

    Cofactori

    Divalent cations. Probably zinc By similarity.By similarity

    GO - Molecular functioni

    1. cAMP response element binding protein binding Source: MGI
    2. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: MGI

    GO - Biological processi

    1. C-terminal protein methylation Source: MGI
    2. in utero embryonic development Source: MGI
    3. liver development Source: MGI
    4. multicellular organism growth Source: MGI
    5. positive regulation of cell proliferation Source: MGI
    6. protein localization Source: MGI
    7. protein methylation Source: MGI
    8. regulation of Ras protein signal transduction Source: MGI
    9. regulation of RNA biosynthetic process Source: GOC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    SABIO-RKQ9EQK7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
    Alternative name(s):
    Isoprenylcysteine carboxylmethyltransferase
    Prenylated protein carboxyl methyltransferase
    Short name:
    PPMT
    Prenylcysteine carboxyl methyltransferase
    Short name:
    pcCMT
    Gene namesi
    Name:Icmt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1888594. Icmt.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 283283Protein-S-isoprenylcysteine O-methyltransferasePRO_0000209895Add
    BLAST

    Proteomic databases

    PaxDbiQ9EQK7.
    PRIDEiQ9EQK7.

    PTM databases

    PhosphoSiteiQ9EQK7.

    Expressioni

    Tissue specificityi

    Highly enriched in adult cerebellum, with a low level expression in other brain regions.

    Developmental stagei

    During cerebellar development expression was low before postnatal day P6 and dramatically increased after P12.

    Gene expression databases

    CleanExiMM_ICMT.
    GenevestigatoriQ9EQK7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EQK7.
    SMRiQ9EQK7. Positions 181-274.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 408LumenalSequence Analysis
    Topological domaini59 – 6810CytoplasmicSequence Analysis
    Topological domaini87 – 915LumenalSequence Analysis
    Topological domaini112 – 13019CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini149 – 1535LumenalSequence Analysis
    Topological domaini174 – 21138CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini228 – 2281LumenalSequence Analysis
    Topological domaini244 – 28340CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3217HelicalSequence AnalysisAdd
    BLAST
    Transmembranei41 – 5818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei69 – 8618HelicalSequence AnalysisAdd
    BLAST
    Transmembranei92 – 11120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei131 – 14818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei212 – 22716HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 24315HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2020.
    HOVERGENiHBG019034.
    InParanoidiQ9EQK7.
    KOiK00587.
    PhylomeDBiQ9EQK7.

    Family and domain databases

    InterProiIPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view]
    PfamiPF04140. ICMT. 1 hit.
    [Graphical view]
    PROSITEiPS51564. SAM_ICMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EQK7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAARRGSAG SEARLSLATF LLGASVLALP LLTRAGLQGR TGLALYVAGL    50
    NALLLLLYRP PRYQIAIRAC FLGFVFGCGV LLSFSQSSWN HFGWYVCSLS 100
    LFHYSEYLVT AVNNPKSLSL DSFLLNHSLE YTVAALSSWI EFTLENIFWP 150
    ELKQITWLSA TGLLMVVFGE CLRKAAMFTA GSNFNHVVQS EKSDTHTLVT 200
    SGVYAWCRHP SYVGWFYWSI GTQVMLCNPI CGVVYALTVW RFFRDRTEEE 250
    EISLIHFFGE EYLDYKKRVP TGLPFIKGVK VEL 283
    Length:283
    Mass (Da):31,790
    Last modified:June 20, 2001 - v2
    Checksum:iA6C17436EDC27F40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241A → D in AA022288. 1 PublicationCurated
    Sequence conflicti42 – 421G → A1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF209926 mRNA. Translation: AAG48552.1.
    AA022288 mRNA. No translation available.
    RefSeqiNP_598549.1. NM_133788.2.
    UniGeneiMm.277464.

    Genome annotation databases

    GeneIDi57295.
    KEGGimmu:57295.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF209926 mRNA. Translation: AAG48552.1 .
    AA022288 mRNA. No translation available.
    RefSeqi NP_598549.1. NM_133788.2.
    UniGenei Mm.277464.

    3D structure databases

    ProteinModelPortali Q9EQK7.
    SMRi Q9EQK7. Positions 181-274.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9EQK7.

    Proteomic databases

    PaxDbi Q9EQK7.
    PRIDEi Q9EQK7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 57295.
    KEGGi mmu:57295.

    Organism-specific databases

    CTDi 23463.
    MGIi MGI:1888594. Icmt.

    Phylogenomic databases

    eggNOGi COG2020.
    HOVERGENi HBG019034.
    InParanoidi Q9EQK7.
    KOi K00587.
    PhylomeDBi Q9EQK7.

    Enzyme and pathway databases

    SABIO-RK Q9EQK7.

    Miscellaneous databases

    NextBioi 313651.
    PROi Q9EQK7.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_ICMT.
    Genevestigatori Q9EQK7.

    Family and domain databases

    InterProi IPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view ]
    Pfami PF04140. ICMT. 1 hit.
    [Graphical view ]
    PROSITEi PS51564. SAM_ICMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Polyglutamine expansion down-regulates specific neuronal genes before pathologic changes in SCA1."
      Lin X., Antalffy B., Kang D., Orr H.T., Zoghbi H.Y.
      Nat. Neurosci. 3:157-163(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-283.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-153.
      Strain: C57BL/6J.
      Tissue: Placenta.

    Entry informationi

    Entry nameiICMT_MOUSE
    AccessioniPrimary (citable) accession number: Q9EQK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: June 20, 2001
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3