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Protein

Major vault protein

Gene

Mvp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • ERBB signaling pathway Source: MGI
  • negative regulation of protein autophosphorylation Source: MGI
  • negative regulation of protein tyrosine kinase activity Source: MGI
  • negative regulation of signaling Source: MGI
  • protein activation cascade Source: UniProtKB
  • regulation of signaling Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Major vault protein
Short name:
MVP
Gene namesi
Name:Mvp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1925638. Mvp.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001589812 – 861Major vault proteinAdd BLAST860

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei421PhosphoserineBy similarity1
Cross-linki444Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylated on Tyr residues after EGF stimulation.By similarity
Dephosphorylated by PTPN11.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9EQK5.
MaxQBiQ9EQK5.
PaxDbiQ9EQK5.
PeptideAtlasiQ9EQK5.
PRIDEiQ9EQK5.

2D gel databases

REPRODUCTION-2DPAGEQ9EQK5.

PTM databases

iPTMnetiQ9EQK5.
PhosphoSitePlusiQ9EQK5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030681.
CleanExiMM_MVP.

Interactioni

Subunit structurei

The vault ribonucleoprotein particle is a huge (400 A x 670 A) cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains, PARP4 and one or more vault RNAs (vRNAs). Interacts with PTEN and activated MAPK1. The phosphorylated protein interacts with the SH2 domains of PTPN11 and SRC. Interacts with APEX1 (By similarity). May interact with ZNF540 (By similarity). Interacts with TEP1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9EQK5. 5 interactors.
MINTiMINT-4102629.
STRINGi10090.ENSMUSP00000127250.

Structurei

Secondary structure

1861
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Beta strandi14 – 20Combined sources7
Turni21 – 23Combined sources3
Beta strandi26 – 36Combined sources11
Beta strandi41 – 43Combined sources3
Beta strandi51 – 53Combined sources3
Beta strandi57 – 63Combined sources7
Beta strandi77 – 79Combined sources3
Beta strandi88 – 91Combined sources4
Beta strandi93 – 97Combined sources5
Beta strandi104 – 111Combined sources8
Beta strandi113 – 115Combined sources3
Beta strandi119 – 127Combined sources9
Beta strandi132 – 134Combined sources3
Beta strandi142 – 151Combined sources10
Beta strandi157 – 164Combined sources8
Beta strandi166 – 168Combined sources3
Beta strandi172 – 183Combined sources12
Beta strandi185 – 187Combined sources3
Beta strandi195 – 198Combined sources4
Beta strandi202 – 204Combined sources3
Beta strandi210 – 217Combined sources8
Beta strandi225 – 233Combined sources9
Beta strandi238 – 240Combined sources3
Beta strandi248 – 251Combined sources4
Turni253 – 255Combined sources3
Beta strandi257 – 259Combined sources3
Beta strandi265 – 272Combined sources8
Beta strandi274 – 276Combined sources3
Beta strandi280 – 286Combined sources7
Beta strandi290 – 292Combined sources3
Beta strandi299 – 308Combined sources10
Beta strandi315 – 322Combined sources8
Beta strandi324 – 326Combined sources3
Beta strandi331 – 336Combined sources6
Beta strandi356 – 359Combined sources4
Beta strandi361 – 365Combined sources5
Beta strandi369 – 377Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GF5X-ray2.50A/B1-383[»]
3GNFX-ray2.10B1-383[»]
3GNGX-ray3.00A1-383[»]
ProteinModelPortaliQ9EQK5.
SMRiQ9EQK5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9EQK5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati2 – 56MVP 1Add BLAST55
Repeati57 – 111MVP 2Add BLAST55
Repeati112 – 164MVP 3Add BLAST53
Repeati165 – 217MVP 4Add BLAST53
Repeati218 – 272MVP 5Add BLAST55
Repeati273 – 323MVP 6Add BLAST51
Repeati324 – 379MVP 7Add BLAST56
Repeati380 – 457MVP 8Add BLAST78
Repeati458 – 520MVP 9Add BLAST63

Domaini

MVP 3 mediates interaction with PTEN.By similarity
MVP 4 mediates interaction with PARP4.By similarity

Sequence similaritiesi

Contains 9 MVP (vault) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF6T. Eukaryota.
ENOG410ZCU8. LUCA.
HOVERGENiHBG003499.
InParanoidiQ9EQK5.

Family and domain databases

CDDicd08825. MVP_shoulder. 1 hit.
InterProiIPR021870. MVP_shoulder.
IPR002499. Vault_N.
[Graphical view]
PfamiPF11978. MVP_shoulder. 1 hit.
PF01505. Vault. 4 hits.
[Graphical view]
PROSITEiPS51224. MVP. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EQK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATEEAIIRI PPYHYIHVLD QNSNVSRVEV GPKTYIRQDN ERVLFAPVRM
60 70 80 90 100
VTVPPRHYCI VANPVSRDAQ SSVLFDVTGQ VRLRHADQEI RLAQDPFPLY
110 120 130 140 150
PGELLEKDIT PLQVVLPNTA LHLKALLDFE DKNGDKVMAG DEWLFEGPGT
160 170 180 190 200
YIPQKEVEVV EIIQATVIKQ NQALRLRARK ECFDRDGKER VTGEEWLVRS
210 220 230 240 250
VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ARQNFKDLRG VAHRTGEEWL
260 270 280 290 300
VTVQDTEAHV PDVYEEVLGV VPITTLGPRH YCVILDPMGP DGKNQLGQKR
310 320 330 340 350
VVKGEKSFFL QPGERLERGI QDVYVLSEQQ GLLLKALQPL EEGEGEEKVA
360 370 380 390 400
HQAGDRWLIR GPLEYVPSAK VEVVEERQAI PLDQNEGIYV QDVKTGKVRA
410 420 430 440 450
VIGSTYMLTQ DEVLWEKELP SGVEELLNLG HDPLADRGQK GTAKVLQPSA
460 470 480 490 500
ARNKTRVVSY RVPHNAAVQV YDYRAKRARV VFGPELVSLD PEEQFTVLSL
510 520 530 540 550
SAGRPKRPHA RRALCLLLGP DFFTDVITIE TADHARLQLQ LAYNWHFELK
560 570 580 590 600
NRNDPEETAK LFSVPDFVGD ACKAIASRVR GAVASVTFDD FHKNSARIIR
610 620 630 640 650
MAVFGFEMSE DAGPDGALLP RARDRAVFPQ NGLVVSSVDV QSVEPVDQRT
660 670 680 690 700
RDALQRSVQL AIEITTNSQE AAAKHEAQRL EQEARGRLER QKILDQSEAE
710 720 730 740 750
KARKELLELE AMSMAVESTG NAKAEAESRA EAARIEGEGS VLQAKLKAQA
760 770 780 790 800
LAIETEAELE RVKKVREMEL IYSRAQLELE VSKAQQLADV EAKKFKEMTE
810 820 830 840 850
ALGPGTIRDL AVAGPEMQVK LLQSLGLKST LITDGSSPIN LFNTAFGLLG
860
LGSDGQPPVQ K
Length:861
Mass (Da):95,924
Last modified:July 27, 2011 - v4
Checksum:iB722F4FA747CA378
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348K → R in AAG43520 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF210456 mRNA. Translation: AAG43520.1.
AY046318 Genomic DNA. Translation: AAL02325.1.
CH466531 Genomic DNA. Translation: EDL17488.1.
CH466531 Genomic DNA. Translation: EDL17489.1.
BC006709 mRNA. Translation: AAH06709.1.
RefSeqiXP_011240254.1. XM_011241952.2.
UniGeneiMm.228797.

Genome annotation databases

GeneIDi78388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF210456 mRNA. Translation: AAG43520.1.
AY046318 Genomic DNA. Translation: AAL02325.1.
CH466531 Genomic DNA. Translation: EDL17488.1.
CH466531 Genomic DNA. Translation: EDL17489.1.
BC006709 mRNA. Translation: AAH06709.1.
RefSeqiXP_011240254.1. XM_011241952.2.
UniGeneiMm.228797.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GF5X-ray2.50A/B1-383[»]
3GNFX-ray2.10B1-383[»]
3GNGX-ray3.00A1-383[»]
ProteinModelPortaliQ9EQK5.
SMRiQ9EQK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EQK5. 5 interactors.
MINTiMINT-4102629.
STRINGi10090.ENSMUSP00000127250.

PTM databases

iPTMnetiQ9EQK5.
PhosphoSitePlusiQ9EQK5.

2D gel databases

REPRODUCTION-2DPAGEQ9EQK5.

Proteomic databases

EPDiQ9EQK5.
MaxQBiQ9EQK5.
PaxDbiQ9EQK5.
PeptideAtlasiQ9EQK5.
PRIDEiQ9EQK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi78388.

Organism-specific databases

CTDi9961.
MGIiMGI:1925638. Mvp.

Phylogenomic databases

eggNOGiENOG410IF6T. Eukaryota.
ENOG410ZCU8. LUCA.
HOVERGENiHBG003499.
InParanoidiQ9EQK5.

Miscellaneous databases

ChiTaRSiMvp. mouse.
EvolutionaryTraceiQ9EQK5.
PROiQ9EQK5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030681.
CleanExiMM_MVP.

Family and domain databases

CDDicd08825. MVP_shoulder. 1 hit.
InterProiIPR021870. MVP_shoulder.
IPR002499. Vault_N.
[Graphical view]
PfamiPF11978. MVP_shoulder. 1 hit.
PF01505. Vault. 4 hits.
[Graphical view]
PROSITEiPS51224. MVP. 8 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMVP_MOUSE
AccessioniPrimary (citable) accession number: Q9EQK5
Secondary accession number(s): Q922X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 104 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.