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Protein

Zinc finger protein 384

Gene

Znf384

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds the consensus DNA sequence [GC]AAAAA. Seems to bind and regulate the promoters of MMP1, MMP3, MMP7 and COL1A1.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri229 – 25123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri257 – 27923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri285 – 30723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri318 – 34023C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri346 – 36823C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39825C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri404 – 42623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri434 – 45623C2H2-type 8PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding Source: RGD
  • SH3 domain binding Source: RGD

GO - Biological processi

  • nucleocytoplasmic transport Source: RGD
  • positive regulation of protein secretion Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 384
Alternative name(s):
Cas-associated zinc finger protein
Nuclear matrix transcription factor 4
Short name:
Nuclear matrix protein 4
Gene namesi
Name:Znf384
Synonyms:Ciz, Nmp4, Zfp384
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708346. Zfp384.

Subcellular locationi

GO - Cellular componenti

  • focal adhesion Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Zinc finger protein 384PRO_0000047553Add
BLAST

Proteomic databases

PaxDbiQ9EQJ4.
PRIDEiQ9EQJ4.

Expressioni

Tissue specificityi

Expressed in osteocytes, osteoblasts, and chondrocytes in bone.

Interactioni

Subunit structurei

Interacts with BCAR1.1 Publication

GO - Molecular functioni

  • SH3 domain binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052839.

Structurei

3D structure databases

ProteinModelPortaliQ9EQJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi462 – 52463Gln-richAdd
BLAST
Compositional biasi467 – 50640Ala-richAdd
BLAST

Sequence similaritiesi

Contains 8 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri229 – 25123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri257 – 27923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri285 – 30723C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri318 – 34023C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri346 – 36823C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39825C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri404 – 42623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri434 – 45623C2H2-type 8PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000233753.
HOVERGENiHBG058173.
InParanoidiQ9EQJ4.
PhylomeDBiQ9EQJ4.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 8 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9EQJ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEESHFNSNP YFWPSIPTVS GQIENTMFIN KMKDQLLPEK GCGLAPPHYP
60 70 80 90 100
TLLTVPASVS LSSGISMDTE SKSEQLTPHS QASVTQNITV VPVPSTGLMT
110 120 130 140 150
AGVSCSQRWR REGSQSRGPG LVITSPSGSL VTTASSAQTF PISTPMIVSA
160 170 180 190 200
LPPGSQALQV VPDLSKKVAS TLTEEGGGGG GGGGTVAPPK PPRGRKKKRM
210 220 230 240 250
LESGLPEMND PYVLAPGDDD DHQKDGKTYR CRMCSLTFYS KSEMQIHSKS
260 270 280 290 300
HTETKPHKCP HCSKTFANSS YLAQHIRIHS GAKPYSCNFC EKSFRQLSHL
310 320 330 340 350
QQHTRIHSKM HTETIKPHKC PHCSKTFANT SYLAQHLRIH SGAKPYNCSY
360 370 380 390 400
CQKAFRQLSH LQQHTRIHTG DRPYKCAHPG CEKAFTQLSN LQSHRRQHNK
410 420 430 440 450
DKPFKCHNCH RAYTDAASLE AHLSTHTVKH AKVYTCTICS RAYTSETYLM
460 470 480 490 500
KHMRKHNPPD LQQQVQAAAA AAAVAQAQAQ AQAQAQAQAQ AQAQAQAQAQ
510 520 530 540 550
AQASQASQQQ QQQQPPPPQP PHFQSPGAAP QGGGGGDSNQ NPPPQCSFDL
560 570
TPYKPAEHHK DICLTVTTST IQVEHLASS
Length:579
Mass (Da):63,139
Last modified:March 1, 2001 - v1
Checksum:iFBC242E0D1050C45
GO
Isoform 2 (identifier: Q9EQJ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-118: Missing.

Show »
Length:563
Mass (Da):61,278
Checksum:i73A717D8F5457A96
GO
Isoform 3 (identifier: Q9EQJ4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     301-361: Missing.

Show »
Length:518
Mass (Da):56,026
Checksum:i8DA4BC4EE8BEE030
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1792GG → RS in BAA89664 (PubMed:10669742).Curated
Sequence conflicti576 – 5772LA → WP in BAA89664 (PubMed:10669742).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei103 – 11816Missing in isoform 2. 1 PublicationVSP_006921Add
BLAST
Alternative sequencei301 – 36161Missing in isoform 3. 1 PublicationVSP_006922Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019281 mRNA. Translation: BAA89664.1.
AF216804 mRNA. Translation: AAG40582.1.
AF216805 mRNA. Translation: AAG40583.1.
AF216806 mRNA. Translation: AAG40584.1.
RefSeqiNP_001030002.1. NM_001034830.1.
NP_001030003.1. NM_001034831.1.
NP_596920.2. NM_133429.2.
UniGeneiRn.9637.

Genome annotation databases

GeneIDi171018.
KEGGirno:171018.
UCSCiRGD:708346. rat. [Q9EQJ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019281 mRNA. Translation: BAA89664.1.
AF216804 mRNA. Translation: AAG40582.1.
AF216805 mRNA. Translation: AAG40583.1.
AF216806 mRNA. Translation: AAG40584.1.
RefSeqiNP_001030002.1. NM_001034830.1.
NP_001030003.1. NM_001034831.1.
NP_596920.2. NM_133429.2.
UniGeneiRn.9637.

3D structure databases

ProteinModelPortaliQ9EQJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052839.

Proteomic databases

PaxDbiQ9EQJ4.
PRIDEiQ9EQJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171018.
KEGGirno:171018.
UCSCiRGD:708346. rat. [Q9EQJ4-1]

Organism-specific databases

CTDi269800.
RGDi708346. Zfp384.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000233753.
HOVERGENiHBG058173.
InParanoidiQ9EQJ4.
PhylomeDBiQ9EQJ4.

Miscellaneous databases

NextBioi621509.
PROiQ9EQJ4.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 8 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "CIZ, a zinc finger protein that interacts with p130cas and activates the expression of matrix metalloproteinases."
    Nakamoto T., Yamagata T., Sakai R., Ogawa S., Honda H., Ueno H., Hirano N., Yazaki Y., Hirai H.
    Mol. Cell. Biol. 20:1649-1658(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, DNA-BINDING, INTERACTION WITH BCAR1.
  2. "Cloning and functional analysis of a family of nuclear matrix transcription factors (NP/NMP4) that regulate type I collagen expression in osteoblasts."
    Thunyakitpisal P., Alvarez M., Tokunaga K., Onyia J.E., Hock J., Ohashi N., Feister H., Rhodes S.J., Bidwell J.P.
    J. Bone Miner. Res. 16:10-23(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiZN384_RAT
AccessioniPrimary (citable) accession number: Q9EQJ4
Secondary accession number(s): Q9EQJ2, Q9EQJ3, Q9JMJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: March 1, 2001
Last modified: December 9, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.