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Protein

Two pore calcium channel protein 1

Gene

Tpcn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca2+ channels (VDCC) across the lysosomal and endosomal membrane.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi1.A.1.11.22. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Two pore calcium channel protein 1
Alternative name(s):
Voltage-dependent calcium channel protein TPC1
Gene namesi
Name:Tpcn1
Synonyms:Kiaa1169, Tpc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2182472. Tpcn1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 113113CytoplasmicSequence analysisAdd
BLAST
Transmembranei114 – 13421Helical; Name=S1 of repeat ISequence analysisAdd
BLAST
Topological domaini135 – 1373ExtracellularSequence analysis
Transmembranei138 – 15821Helical; Name=S2 of repeat ISequence analysisAdd
BLAST
Topological domaini159 – 17214CytoplasmicSequence analysisAdd
BLAST
Transmembranei173 – 19321Helical; Name=S3 of repeat ISequence analysisAdd
BLAST
Topological domaini194 – 2029ExtracellularSequence analysis
Transmembranei203 – 22119Helical; Name=S4 of repeat ISequence analysisAdd
BLAST
Topological domaini222 – 23514CytoplasmicSequence analysisAdd
BLAST
Transmembranei236 – 25621Helical; Name=S5 of repeat ISequence analysisAdd
BLAST
Topological domaini257 – 2637ExtracellularSequence analysis
Intramembranei264 – 28724Helical; Pore-formingSequence analysisAdd
BLAST
Topological domaini288 – 29811ExtracellularSequence analysisAdd
BLAST
Transmembranei299 – 31921Helical; Name=S6 of repeat ISequence analysisAdd
BLAST
Topological domaini320 – 445126CytoplasmicSequence analysisAdd
BLAST
Transmembranei446 – 46621Helical; Name=S1 of repeat IISequence analysisAdd
BLAST
Topological domaini467 – 48014ExtracellularSequence analysisAdd
BLAST
Transmembranei481 – 50121Helical; Name=S2 of repeat IISequence analysisAdd
BLAST
Topological domaini502 – 5043CytoplasmicSequence analysis
Transmembranei505 – 52723Helical; Name=S3 of repeat IISequence analysisAdd
BLAST
Topological domaini528 – 5358ExtracellularSequence analysis
Transmembranei536 – 55015Helical; Name=S4 of repeat IISequence analysisAdd
BLAST
Topological domaini551 – 56919CytoplasmicSequence analysisAdd
BLAST
Transmembranei570 – 59021Helical; Name=S5 of repeat IISequence analysisAdd
BLAST
Topological domaini591 – 63040ExtracellularSequence analysisAdd
BLAST
Intramembranei631 – 65424Helical; Pore-formingSequence analysisAdd
BLAST
Topological domaini655 – 67117ExtracellularSequence analysisAdd
BLAST
Transmembranei672 – 69221Helical; Name=S6 of repeat IISequence analysisAdd
BLAST
Topological domaini693 – 817125CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 817817Two pore calcium channel protein 1PRO_0000276854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi470 – 4701N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9EQJ0.
PaxDbiQ9EQJ0.
PRIDEiQ9EQJ0.

PTM databases

iPTMnetiQ9EQJ0.
PhosphoSiteiQ9EQJ0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032741.
CleanExiMM_TPCN1.
GenevisibleiQ9EQJ0. MM.

Interactioni

Subunit structurei

Dimer.Curated

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042188.

Structurei

3D structure databases

ProteinModelPortaliQ9EQJ0.
SMRiQ9EQJ0. Positions 178-322, 435-687.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili770 – 79425Sequence analysisAdd
BLAST

Domaini

Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INF7. Eukaryota.
ENOG410XZT8. LUCA.
GeneTreeiENSGT00530000063660.
HOGENOMiHOG000006773.
HOVERGENiHBG080605.
InParanoidiQ9EQJ0.
KOiK16896.
OMAiAIQHAYH.
OrthoDBiEOG091G038K.
PhylomeDBiQ9EQJ0.
TreeFamiTF328550.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EQJ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVSLDDDVP LILTLDEAES APLPPSNSLG QEQLPSKNGG SHSIHNSQVP
60 70 80 90 100
SLVSGADSPP SSPTGHNWEM NYQEAAIYLQ EGQNNDKFFT HPKDARALAA
110 120 130 140 150
YLFVHNHFFY MMELLTALLL LLLSLCESPA VPVLKLHTYV HATLELFALM
160 170 180 190 200
VVVFELCMKL RWLGFHTFVR HKRTMVKTSV LVVQFIEAIV VLVRQTSHVR
210 220 230 240 250
VTRALRCIFL VDCRYCGGVR RNLRQIFQSL PPFMDILLLL LFFMIIFAIL
260 270 280 290 300
GFYLFSTNPS DPYFSTLENS IVNLFVLLTT ANFPDVMMPS YSRNPWSCVF
310 320 330 340 350
FIVYLSIELY FIMNLLLAVV FDTFNDIEKH KFKSLLLHKR TAIQHAYGLL
360 370 380 390 400
ASQRRPAGIS YRQFEGLMRF YKPRMSARER FLTFKALNQS NTPLLSLKDF
410 420 430 440 450
YDIYEVAALQ WKAKRNRQHW FDELPRTAFL IFKGINILVN SKAFQYFMYL
460 470 480 490 500
VVAVNGVWIL VETFMLKGGN FTSKHVPWSY LVFLTIYGVE LFMKVAGLGP
510 520 530 540 550
VEYLSSGWNL FDFSVTAFAF LGLLALTLNM EPFYFIVVLR PLQLLRLFKL
560 570 580 590 600
KKRYRNVLDT MFELLPRMAS LGLTLLTFYY SFAIVGMEFF NGRLTPNCCN
610 620 630 640 650
TSTVADAYRF INHTVGNKTK VEEGYYYLNN FDNILNSFVT LFELTVVNNW
660 670 680 690 700
YIIMEGVTSQ TSHWSRLYFM TFYIVTMVVM TIIVAFILEA FVFRMNYSRK
710 720 730 740 750
SQDSEVDSGI VIEKEMSKEE LMAVLELYRE ERGTSSDVTR LLDTLSQMEK
760 770 780 790 800
YQQNSMVFLG RRSRTKSDLS LKMYQEEIQE WYEEHAREQE QQKLRGSVPG
810
PAAQQPPGSR QRSQTVT
Length:817
Mass (Da):94,496
Last modified:March 1, 2001 - v1
Checksum:i269930E1D1136B10
GO
Isoform 2 (identifier: Q9EQJ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     449-488: YLVVAVNGVW...SYLVFLTIYG → CKCDPSQLWA...STGFCPQYFS
     489-817: Missing.

Show »
Length:488
Mass (Da):56,200
Checksum:iFABAE9BFF61FB810
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei449 – 48840YLVVA…LTIYG → CKCDPSQLWAPVLLSPSFLK VERQTIQKTPSTGFCPQYFS in isoform 2. 1 PublicationVSP_023006Add
BLAST
Alternative sequencei489 – 817329Missing in isoform 2. 1 PublicationVSP_023007Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217002 mRNA. Translation: AAG44100.1.
AK077411 mRNA. Translation: BAE43360.1.
AK148129 mRNA. Translation: BAE28363.1.
BC058951 mRNA. Translation: AAH58951.1.
AK129303 Transcribed RNA. Translation: BAC98113.2.
CCDSiCCDS39239.1. [Q9EQJ0-1]
RefSeqiNP_665852.1. NM_145853.2. [Q9EQJ0-1]
XP_011246503.1. XM_011248201.1. [Q9EQJ0-1]
UniGeneiMm.114054.

Genome annotation databases

EnsembliENSMUST00000046426; ENSMUSP00000042188; ENSMUSG00000032741. [Q9EQJ0-1]
GeneIDi252972.
KEGGimmu:252972.
UCSCiuc008zhl.1. mouse. [Q9EQJ0-1]
uc008zhn.1. mouse. [Q9EQJ0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217002 mRNA. Translation: AAG44100.1.
AK077411 mRNA. Translation: BAE43360.1.
AK148129 mRNA. Translation: BAE28363.1.
BC058951 mRNA. Translation: AAH58951.1.
AK129303 Transcribed RNA. Translation: BAC98113.2.
CCDSiCCDS39239.1. [Q9EQJ0-1]
RefSeqiNP_665852.1. NM_145853.2. [Q9EQJ0-1]
XP_011246503.1. XM_011248201.1. [Q9EQJ0-1]
UniGeneiMm.114054.

3D structure databases

ProteinModelPortaliQ9EQJ0.
SMRiQ9EQJ0. Positions 178-322, 435-687.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042188.

Protein family/group databases

TCDBi1.A.1.11.22. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiQ9EQJ0.
PhosphoSiteiQ9EQJ0.

Proteomic databases

MaxQBiQ9EQJ0.
PaxDbiQ9EQJ0.
PRIDEiQ9EQJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046426; ENSMUSP00000042188; ENSMUSG00000032741. [Q9EQJ0-1]
GeneIDi252972.
KEGGimmu:252972.
UCSCiuc008zhl.1. mouse. [Q9EQJ0-1]
uc008zhn.1. mouse. [Q9EQJ0-2]

Organism-specific databases

CTDi53373.
MGIiMGI:2182472. Tpcn1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INF7. Eukaryota.
ENOG410XZT8. LUCA.
GeneTreeiENSGT00530000063660.
HOGENOMiHOG000006773.
HOVERGENiHBG080605.
InParanoidiQ9EQJ0.
KOiK16896.
OMAiAIQHAYH.
OrthoDBiEOG091G038K.
PhylomeDBiQ9EQJ0.
TreeFamiTF328550.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ9EQJ0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032741.
CleanExiMM_TPCN1.
GenevisibleiQ9EQJ0. MM.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPC1_MOUSE
AccessioniPrimary (citable) accession number: Q9EQJ0
Secondary accession number(s): Q3V2Z6, Q6ZPW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.