Reviewed, UniProtKB/Swiss-Prot Q9EQH5 (CTBP2_RAT)

Names and origin

Protein names

Recommended name:
    C-terminal-binding protein 2
      Short name=CtBP2

Gene names

Name:

Ctbp2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses By similarity.
Subunit structure	Interacts with HIPK2 and PNN By similarity. Interacts with the transcription factors BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ By similarity.
Subcellular location	Nucleus Potential. Cell junction › synapse By similarity.
Tissue specificity	Isoform 2 is specifically localized in synaptic ribbon (at protein level). Ref.1
Post-translational modification	Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-171 and Ser-177. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Cellular component	Cell junction Nucleus Synapse
Coding sequence diversity	Alternative splicing
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9EQH5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9EQH5-2)

Also known as: Ribeye;

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS

Note: Contains a phosphothreonine at position 171 (By similarity). Contains a phosphoserine at position 177 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	C-terminal-binding protein 2		PRO_0000299356
Sites
	Active site	272	1	Potential
	Active site	301	1	Potential
	Active site	321	1	Potential
Amino acid modifications
	Modified residue	428	1	Phosphoserine; by HIPK2 By similarity
Natural variations
	Alternative sequence	1 – 20	20	MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGVWCELVNHRAQDTES CLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGVLGSRIPW EQVQGQLPALQDAGHLYRES GSKTVLHGQRTHCRAPSPGR YGREQPDSRLGIEVPTYSPN SSQVYNDICERPVDSTHARQ VAPTCLVVDPSSTAPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTVYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLV SSCRTEMQFRRQDRTGPPPR HRSSSFSHRSELLPNDTASL GTTALQFHPAGPLQTPSPRG GDLGRRPSSAPSQHLLETAA TYSAPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSVPLHSSPRT AYSTSYTVPMELLKRERSVT ASPLPSPHASPQLLRKPGAA PVEPAALPPVRQSLHTPHPP YQKVARRTGAPIIVSTMLTP EPS in isoform 2.		VSP_027611

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 403012CDEB2E492B	FASTA	445	48,987
	10 20 30 40 50 60 MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI LKDLATVAFC 70 80 90 100 110 120 DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV IVRIGSGYDN VDIKAAGELG 130 140 150 160 170 180 IAVCNIPSAA VEETADSTVC HILNLYRRNT WLYQALREGT RVQSVEQIRE VASGAARIRG 190 200 210 220 230 240 ETLGLIGFGR TGQAVAVRAK AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS 250 260 270 280 290 300 LHCNLNEHNH HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES 310 320 330 340 350 360 EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP ESLRNCVNKE 370 380 390 400 410 420 FFVTSTPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP PAMEGIIPGG IPVTHNLPTV 430 440 AHPSQAPSPN QPTKHGDNRE HPNEQ
	Isoform 2 (Ribeye). Checksum: 41E08C4148B50424	FASTA	988	107,125
	10 20 30 40 50 60 MPVPSRHINI GRSQSWDAAG WYEGPWENAG PPGRRSSLTY GPGEGVWCEL VNHRAQDTES 70 80 90 100 110 120 CLSREAFYNS LASRKGSVPD FTFYDSRQAV MSGRGSVLPQ DYYGDPSRGT RVPKEPPFYR 130 140 150 160 170 180 DPGTSRPVPS YGVLGSRIPW EQVQGQLPAL QDAGHLYRES GSKTVLHGQR THCRAPSPGR 190 200 210 220 230 240 YGREQPDSRL GIEVPTYSPN SSQVYNDICE RPVDSTHARQ VAPTCLVVDP SSTAPTENST 250 260 270 280 290 300 GVAPGSLNRG YGPTRESIHS KLAYENYEAD LSTFQGPGGK RTVYPEFLAL LRAEGVAEAT 310 320 330 340 350 360 LAALLQQGFD SPAVLATLED ADIKSVAPNL GQARVLSRLV SSCRTEMQFR RQDRTGPPPR 370 380 390 400 410 420 HRSSSFSHRS ELLPNDTASL GTTALQFHPA GPLQTPSPRG GDLGRRPSSA PSQHLLETAA 430 440 450 460 470 480 TYSAPVVGSQ TPHLPSNSGY SSPTPCALTA RLASSYPSQA GVALTANPGP SVPLHSSPRT 490 500 510 520 530 540 AYSTSYTVPM ELLKRERSVT ASPLPSPHAS PQLLRKPGAA PVEPAALPPV RQSLHTPHPP 550 560 570 580 590 600 YQKVARRTGA PIIVSTMLTP EPSIRPQIMN GPLHPRPLVA LLDGRDCTVE MPILKDLATV 610 620 630 640 650 660 AFCDAQSTQE IHEKVLNEAV GAMMYHTITL TREDLEKFKA LRVIVRIGSG YDNVDIKAAG 670 680 690 700 710 720 ELGIAVCNIP SAAVEETADS TVCHILNLYR RNTWLYQALR EGTRVQSVEQ IREVASGAAR 730 740 750 760 770 780 IRGETLGLIG FGRTGQAVAV RAKAFGFSVI FYDPYLQDGI ERSLGVQRVY TLQDLLYQSD 790 800 810 820 830 840 CVSLHCNLNE HNHHLINDFT IKQMRQGAFL VNAARGGLVD EKALAQALKE GRIRGAALDV 850 860 870 880 890 900 HESEPFSFAQ GPLKDAPNLI CTPHTAWYSE QASLEMREAA ATEIRRAITG RIPESLRNCV 910 920 930 940 950 960 NKEFFVTSTP WSVIDQQAIH PELNGATYRY PPGIVGVAPG GLPPAMEGII PGGIPVTHNL 970 980 PTVAHPSQAP SPNQPTKHGD NREHPNEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RIBEYE, a component of synaptic ribbons: a protein's journey through evolution provides insight into synaptic ribbon function." Schmitz F., Koenigstorfer A., Suedhof T.C. Neuron 28:857-872(2000) [PubMed: 11163272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
[2]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF222712 mRNA. Translation: AAG45952.1. AABR03001613 Genomic DNA. No translation available. AABR03000195 Genomic DNA. No translation available.
IPI	IPI00189984. IPI00476123.
RefSeq	NP_445787.1.
UniGene	Rn.138124
3D structure databases
HSSP	HSSP built from PDB template 1MX3 based on UniProtKB Q13363.
SMR	Q9EQH5. Positions 576-905.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000017326. Rattus norvegicus. [Contig view]
GeneID	81717.
KEGG	rno:81717.
NMPDR	fig|10116.3.peg.3145.
Organism-specific databases
RGD	68372. Ctbp2.
Phylogenomic databases
HOVERGEN	Q9EQH5.
OMA	Q9EQH5. GHLYRDP.
Gene expression databases
ArrayExpress	Q9EQH5.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615359.

Entry information

Entry name

CTBP2_RAT

Accession

Primary (citable) accession number: Q9EQH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	June 16, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)