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Protein

C-terminal-binding protein 2

Gene

Ctbp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei106 – 1061NADBy similarity
Binding sitei210 – 2101NADBy similarity
Active sitei272 – 2721By similarity
Binding sitei296 – 2961NADBy similarity
Active sitei301 – 3011By similarity
Active sitei321 – 3211Proton donorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi186 – 1916NADBy similarity
Nucleotide bindingi243 – 2497NADBy similarity
Nucleotide bindingi270 – 2723NADBy similarity
Nucleotide bindingi321 – 3244NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 2
Short name:
CtBP2
Gene namesi
Name:Ctbp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi68372. Ctbp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Nucleus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445C-terminal-binding protein 2PRO_0000299356Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei428 – 4281Phosphoserine; by HIPK2By similarity

Post-translational modificationi

Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9EQH5.
PRIDEiQ9EQH5.

PTM databases

PhosphoSiteiQ9EQH5.

Expressioni

Tissue specificityi

Isoform 2 is specifically localized in synaptic ribbon (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000017326.
GenevisibleiQ9EQH5. RN.

Interactioni

Subunit structurei

Interacts with HIPK2, ZNF217 and PNN (By similarity). Interacts with the transcription factors BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression (By similarity). Interacts with FAM195B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249593. 1 interaction.
STRINGi10116.ENSRNOP00000023404.

Structurei

3D structure databases

ProteinModelPortaliQ9EQH5.
SMRiQ9EQH5. Positions 33-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOGENOMiHOG000136701.
HOVERGENiHBG001898.
InParanoidiQ9EQH5.
KOiK04496.
OMAiEDADIKC.
OrthoDBiEOG091G08GS.
PhylomeDBiQ9EQH5.
TreeFamiTF313593.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EQH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI
60 70 80 90 100
LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV
110 120 130 140 150
IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTVC HILNLYRRNT
160 170 180 190 200
WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK
210 220 230 240 250
AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH
260 270 280 290 300
HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES
310 320 330 340 350
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP
360 370 380 390 400
ESLRNCVNKE FFVTSTPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP
410 420 430 440
PAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ
Length:445
Mass (Da):48,987
Last modified:September 11, 2007 - v2
Checksum:i403012CDEB2E492B
GO
Isoform 2 (identifier: Q9EQH5-2) [UniParc]FASTAAdd to basket
Also known as: Ribeye

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS

Show »
Length:988
Mass (Da):107,125
Checksum:i41E08C4148B50424
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGVWCELVNHRAQDTES CLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGVLGSRIPW EQVQGQLPALQDAGHLYRES GSKTVLHGQRTHCRAPSPGR YGREQPDSRLGIEVPTYSPN SSQVYNDICERPVDSTHARQ VAPTCLVVDPSSTAPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTVYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLV SSCRTEMQFRRQDRTGPPPR HRSSSFSHRSELLPNDTASL GTTALQFHPAGPLQTPSPRG GDLGRRPSSAPSQHLLETAA TYSAPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSVPLHSSPRT AYSTSYTVPMELLKRERSVT ASPLPSPHASPQLLRKPGAA PVEPAALPPVRQSLHTPHPP YQKVARRTGAPIIVSTMLTP EPS in isoform 2. 1 PublicationVSP_027611Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222712 mRNA. Translation: AAG45952.1.
AABR03001613 Genomic DNA. No translation available.
AABR03000195 Genomic DNA. No translation available.
RefSeqiNP_445787.1. NM_053335.1. [Q9EQH5-2]
UniGeneiRn.138124.

Genome annotation databases

EnsembliENSRNOT00000023404; ENSRNOP00000023404; ENSRNOG00000017326. [Q9EQH5-2]
ENSRNOT00000023574; ENSRNOP00000023574; ENSRNOG00000017326. [Q9EQH5-1]
GeneIDi81717.
KEGGirno:81717.
UCSCiRGD:68372. rat. [Q9EQH5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222712 mRNA. Translation: AAG45952.1.
AABR03001613 Genomic DNA. No translation available.
AABR03000195 Genomic DNA. No translation available.
RefSeqiNP_445787.1. NM_053335.1. [Q9EQH5-2]
UniGeneiRn.138124.

3D structure databases

ProteinModelPortaliQ9EQH5.
SMRiQ9EQH5. Positions 33-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249593. 1 interaction.
STRINGi10116.ENSRNOP00000023404.

PTM databases

PhosphoSiteiQ9EQH5.

Proteomic databases

PaxDbiQ9EQH5.
PRIDEiQ9EQH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023404; ENSRNOP00000023404; ENSRNOG00000017326. [Q9EQH5-2]
ENSRNOT00000023574; ENSRNOP00000023574; ENSRNOG00000017326. [Q9EQH5-1]
GeneIDi81717.
KEGGirno:81717.
UCSCiRGD:68372. rat. [Q9EQH5-1]

Organism-specific databases

CTDi1488.
RGDi68372. Ctbp2.

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOGENOMiHOG000136701.
HOVERGENiHBG001898.
InParanoidiQ9EQH5.
KOiK04496.
OMAiEDADIKC.
OrthoDBiEOG091G08GS.
PhylomeDBiQ9EQH5.
TreeFamiTF313593.

Miscellaneous databases

PROiQ9EQH5.

Gene expression databases

BgeeiENSRNOG00000017326.
GenevisibleiQ9EQH5. RN.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTBP2_RAT
AccessioniPrimary (citable) accession number: Q9EQH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: September 7, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.