Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription initiation factor TFIID subunit 8

Gene

Taf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex (By similarity). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.By similarity2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 8
Alternative name(s):
Protein taube nuss
TBP-associated factor 8
Gene namesi
Name:Taf8
Synonyms:Tbn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1926879. Taf8.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death during early embryonic development. The inner cell mass cells of mutant embryos died of apoptosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 308307Transcription initiation factor TFIID subunit 8PRO_0000315398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei128 – 1281PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9EQH4.
MaxQBiQ9EQH4.
PaxDbiQ9EQH4.
PRIDEiQ9EQH4.

PTM databases

iPTMnetiQ9EQH4.
PhosphoSiteiQ9EQH4.

Expressioni

Tissue specificityi

Low level of expression throughout the brain with slightly higher expression in the hippocampus.

Developmental stagei

Expressed ubiquitously at very low levels throughout embryonic development. Higher levels of expression seen in inner cell mass and heart.1 Publication

Gene expression databases

BgeeiQ9EQH4.
GenevisibleiQ9EQH4. MM.

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063201.

Structurei

3D structure databases

ProteinModelPortaliQ9EQH4.
SMRiQ9EQH4. Positions 26-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 10268Histone-foldAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi292 – 30514Nuclear localization signalSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the TAF8 family.Curated
Contains 1 histone-fold domain.Curated

Phylogenomic databases

eggNOGiKOG4336. Eukaryota.
ENOG410ZRN5. LUCA.
GeneTreeiENSGT00390000017567.
HOGENOMiHOG000007034.
HOVERGENiHBG105411.
InParanoidiQ9EQH4.
KOiK14649.
OMAiNVIDNPY.
OrthoDBiEOG783MVS.
PhylomeDBiQ9EQH4.
TreeFamiTF316311.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR006565. BTP.
IPR009072. Histone-fold.
IPR019473. TFIID_su8_C.
[Graphical view]
PfamiPF07524. Bromo_TP. 1 hit.
PF10406. TAF8_C. 1 hit.
[Graphical view]
SMARTiSM00576. BTP. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EQH4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTAAGPGG SGTRPGSKQS TNPADNYHLA RRRTLQVVVS SLLTEAGFES
60 70 80 90 100
AEKASVETLT EMLQSYISEI GRSAKSYCEH TARTQPTLSD IVVTLVEMGF
110 120 130 140 150
NVDTLPAYAK RSQRMVITAP PVTNQPVTPK ALTAGQNRPH PPHIPSHFPE
160 170 180 190 200
FPDPHTYIKT PTYREPVSDY QILREKAASQ RRDVERALTR FMAKTGETQS
210 220 230 240 250
LFKDDVSTFP LIAARPFTIP YLTALLPSEL EIQQMEETDS SEQEEQTDTE
260 270 280 290 300
NNALHISTDD SGAEKESASV LQQSSSLSGS RNGEESVIDN PYLRPVKKPK

IRRKKSLS
Length:308
Mass (Da):33,988
Last modified:March 1, 2001 - v1
Checksum:iFD527CD7905CC8D9
GO
Isoform 2 (identifier: Q9EQH4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     306-308: Missing.

Show »
Length:305
Mass (Da):33,701
Checksum:iC7905CC8D9ED1AD4
GO
Isoform 3 (identifier: Q9EQH4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-265: DDSGAEK → GHPEASA
     266-308: Missing.

Show »
Length:265
Mass (Da):29,196
Checksum:i9EC61183C9051CFB
GO
Isoform 4 (identifier: Q9EQH4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-308: DDSGAEKESA...PKIRRKKSLS → VGPPLLPASQ...LLGSHTFSRW

Show »
Length:322
Mass (Da):35,440
Checksum:iE05BE93D76FE5D7A
GO
Isoform 5 (identifier: Q9EQH4-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-221: AARPFTIPY → SDQHLIKAP
     222-308: Missing.

Note: No experimental confirmation available.
Show »
Length:221
Mass (Da):24,361
Checksum:i924CADAB54196D11
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271V → L in BAC36363 (PubMed:16141072).Curated
Sequence conflicti153 – 1531D → V in BAC34001 (PubMed:16141072).Curated
Sequence conflicti236 – 2361E → G in BAC34001 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei213 – 2219AARPFTIPY → SDQHLIKAP in isoform 5. 1 PublicationVSP_030550
Alternative sequencei222 – 30887Missing in isoform 5. 1 PublicationVSP_030551Add
BLAST
Alternative sequencei259 – 30850DDSGA…KKSLS → VGPPLLPASQQSQNSLSHIL PLPLGTEQGLPQVSGWLLQA CMMGCVSSLHHETHLLGSHT FSRW in isoform 4. 1 PublicationVSP_030552Add
BLAST
Alternative sequencei259 – 2657DDSGAEK → GHPEASA in isoform 3. 1 PublicationVSP_030553
Alternative sequencei266 – 30843Missing in isoform 3. 1 PublicationVSP_030554Add
BLAST
Alternative sequencei306 – 3083Missing in isoform 2. 1 PublicationVSP_030555

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222802 mRNA. Translation: AAG01682.1.
AK045108 mRNA. Translation: BAC32225.1.
AK049949 mRNA. Translation: BAC34001.1.
AK076486 mRNA. Translation: BAC36363.1.
AK086656 mRNA. Translation: BAC39712.1.
AK162110 mRNA. Translation: BAE36731.1.
BC057895 mRNA. Translation: AAH57895.1.
CCDSiCCDS37642.1. [Q9EQH4-1]
RefSeqiNP_071298.1. NM_022015.3. [Q9EQH4-1]
XP_006524827.1. XM_006524764.1. [Q9EQH4-4]
XP_006524828.1. XM_006524765.2. [Q9EQH4-2]
XP_006524830.1. XM_006524767.2. [Q9EQH4-3]
UniGeneiMm.275901.

Genome annotation databases

EnsembliENSMUST00000067103; ENSMUSP00000063201; ENSMUSG00000023980. [Q9EQH4-1]
GeneIDi63856.
KEGGimmu:63856.
UCSCiuc008cvh.1. mouse. [Q9EQH4-1]
uc008cvj.1. mouse. [Q9EQH4-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222802 mRNA. Translation: AAG01682.1.
AK045108 mRNA. Translation: BAC32225.1.
AK049949 mRNA. Translation: BAC34001.1.
AK076486 mRNA. Translation: BAC36363.1.
AK086656 mRNA. Translation: BAC39712.1.
AK162110 mRNA. Translation: BAE36731.1.
BC057895 mRNA. Translation: AAH57895.1.
CCDSiCCDS37642.1. [Q9EQH4-1]
RefSeqiNP_071298.1. NM_022015.3. [Q9EQH4-1]
XP_006524827.1. XM_006524764.1. [Q9EQH4-4]
XP_006524828.1. XM_006524765.2. [Q9EQH4-2]
XP_006524830.1. XM_006524767.2. [Q9EQH4-3]
UniGeneiMm.275901.

3D structure databases

ProteinModelPortaliQ9EQH4.
SMRiQ9EQH4. Positions 26-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063201.

PTM databases

iPTMnetiQ9EQH4.
PhosphoSiteiQ9EQH4.

Proteomic databases

EPDiQ9EQH4.
MaxQBiQ9EQH4.
PaxDbiQ9EQH4.
PRIDEiQ9EQH4.

Protocols and materials databases

DNASUi63856.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067103; ENSMUSP00000063201; ENSMUSG00000023980. [Q9EQH4-1]
GeneIDi63856.
KEGGimmu:63856.
UCSCiuc008cvh.1. mouse. [Q9EQH4-1]
uc008cvj.1. mouse. [Q9EQH4-4]

Organism-specific databases

CTDi129685.
MGIiMGI:1926879. Taf8.

Phylogenomic databases

eggNOGiKOG4336. Eukaryota.
ENOG410ZRN5. LUCA.
GeneTreeiENSGT00390000017567.
HOGENOMiHOG000007034.
HOVERGENiHBG105411.
InParanoidiQ9EQH4.
KOiK14649.
OMAiNVIDNPY.
OrthoDBiEOG783MVS.
PhylomeDBiQ9EQH4.
TreeFamiTF316311.

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Miscellaneous databases

PROiQ9EQH4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EQH4.
GenevisibleiQ9EQH4. MM.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR006565. BTP.
IPR009072. Histone-fold.
IPR019473. TFIID_su8_C.
[Graphical view]
PfamiPF07524. Bromo_TP. 1 hit.
PF10406. TAF8_C. 1 hit.
[Graphical view]
SMARTiSM00576. BTP. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Taube nuss is a novel gene essential for the survival of pluripotent cells of early mouse embryos."
    Voss A.K., Thomas T., Petrou P., Anastassiadis K., Schoeler H., Gruss P.
    Development 127:5449-5461(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Egg, Head and Hippocampus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
    Strain: 129.
    Tissue: Mammary tumor.
  4. "The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis."
    Guermah M., Ge K., Chiang C.-M., Roeder R.G.
    Mol. Cell 12:991-1001(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTAF8_MOUSE
AccessioniPrimary (citable) accession number: Q9EQH4
Secondary accession number(s): Q3TSE4
, Q6PES8, Q8C382, Q8C664, Q8C7L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

'Taube nuss' means "empty nut' in German.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.