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Protein

Transcription initiation factor TFIID subunit 8

Gene

Taf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex (By similarity). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.By similarity2 Publications

Miscellaneous

'Taube nuss' means 'empty nut' in German.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 8
Alternative name(s):
Protein taube nuss
TBP-associated factor 8
Gene namesi
Name:Taf8
Synonyms:Tbn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1926879. Taf8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death during early embryonic development. The inner cell mass cells of mutant embryos died of apoptosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003153982 – 308Transcription initiation factor TFIID subunit 8Add BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei128PhosphothreonineBy similarity1
Modified residuei269PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9EQH4.
PeptideAtlasiQ9EQH4.
PRIDEiQ9EQH4.

PTM databases

iPTMnetiQ9EQH4.
PhosphoSitePlusiQ9EQH4.

Expressioni

Tissue specificityi

Low level of expression throughout the brain with slightly higher expression in the hippocampus.

Developmental stagei

Expressed ubiquitously at very low levels throughout embryonic development. Higher levels of expression seen in inner cell mass and heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000023980.
GenevisibleiQ9EQH4. MM.

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ9EQH4.
STRINGi10090.ENSMUSP00000063201.

Structurei

3D structure databases

ProteinModelPortaliQ9EQH4.
SMRiQ9EQH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 102Histone-foldAdd BLAST68

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi292 – 305Nuclear localization signalSequence analysisAdd BLAST14

Sequence similaritiesi

Belongs to the TAF8 family.Curated

Phylogenomic databases

eggNOGiKOG4336. Eukaryota.
ENOG410ZRN5. LUCA.
GeneTreeiENSGT00390000017567.
HOGENOMiHOG000007034.
HOVERGENiHBG105411.
InParanoidiQ9EQH4.
KOiK14649.
OMAiLFPTEDN.
OrthoDBiEOG091G0O7T.
PhylomeDBiQ9EQH4.
TreeFamiTF316311.

Family and domain databases

CDDicd08049. TAF8. 1 hit.
InterProiView protein in InterPro
IPR006565. BTP.
IPR009072. Histone-fold.
IPR019473. TFIID_su8_C.
PfamiView protein in Pfam
PF07524. Bromo_TP. 1 hit.
PF10406. TAF8_C. 1 hit.
SMARTiView protein in SMART
SM00576. BTP. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EQH4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTAAGPGG SGTRPGSKQS TNPADNYHLA RRRTLQVVVS SLLTEAGFES
60 70 80 90 100
AEKASVETLT EMLQSYISEI GRSAKSYCEH TARTQPTLSD IVVTLVEMGF
110 120 130 140 150
NVDTLPAYAK RSQRMVITAP PVTNQPVTPK ALTAGQNRPH PPHIPSHFPE
160 170 180 190 200
FPDPHTYIKT PTYREPVSDY QILREKAASQ RRDVERALTR FMAKTGETQS
210 220 230 240 250
LFKDDVSTFP LIAARPFTIP YLTALLPSEL EIQQMEETDS SEQEEQTDTE
260 270 280 290 300
NNALHISTDD SGAEKESASV LQQSSSLSGS RNGEESVIDN PYLRPVKKPK

IRRKKSLS
Length:308
Mass (Da):33,988
Last modified:March 1, 2001 - v1
Checksum:iFD527CD7905CC8D9
GO
Isoform 2 (identifier: Q9EQH4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     306-308: Missing.

Show »
Length:305
Mass (Da):33,701
Checksum:iC7905CC8D9ED1AD4
GO
Isoform 3 (identifier: Q9EQH4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-265: DDSGAEK → GHPEASA
     266-308: Missing.

Show »
Length:265
Mass (Da):29,196
Checksum:i9EC61183C9051CFB
GO
Isoform 4 (identifier: Q9EQH4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-308: DDSGAEKESA...PKIRRKKSLS → VGPPLLPASQ...LLGSHTFSRW

Show »
Length:322
Mass (Da):35,440
Checksum:iE05BE93D76FE5D7A
GO
Isoform 5 (identifier: Q9EQH4-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-221: AARPFTIPY → SDQHLIKAP
     222-308: Missing.

Note: No experimental confirmation available.
Show »
Length:221
Mass (Da):24,361
Checksum:i924CADAB54196D11
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127V → L in BAC36363 (PubMed:16141072).Curated1
Sequence conflicti153D → V in BAC34001 (PubMed:16141072).Curated1
Sequence conflicti236E → G in BAC34001 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030550213 – 221AARPFTIPY → SDQHLIKAP in isoform 5. 1 Publication9
Alternative sequenceiVSP_030551222 – 308Missing in isoform 5. 1 PublicationAdd BLAST87
Alternative sequenceiVSP_030552259 – 308DDSGA…KKSLS → VGPPLLPASQQSQNSLSHIL PLPLGTEQGLPQVSGWLLQA CMMGCVSSLHHETHLLGSHT FSRW in isoform 4. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_030553259 – 265DDSGAEK → GHPEASA in isoform 3. 1 Publication7
Alternative sequenceiVSP_030554266 – 308Missing in isoform 3. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_030555306 – 308Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222802 mRNA. Translation: AAG01682.1.
AK045108 mRNA. Translation: BAC32225.1.
AK049949 mRNA. Translation: BAC34001.1.
AK076486 mRNA. Translation: BAC36363.1.
AK086656 mRNA. Translation: BAC39712.1.
AK162110 mRNA. Translation: BAE36731.1.
BC057895 mRNA. Translation: AAH57895.1.
CCDSiCCDS37642.1. [Q9EQH4-1]
RefSeqiNP_071298.1. NM_022015.3. [Q9EQH4-1]
XP_006524827.1. XM_006524764.2. [Q9EQH4-4]
XP_006524828.1. XM_006524765.3. [Q9EQH4-2]
XP_006524830.1. XM_006524767.3. [Q9EQH4-3]
UniGeneiMm.275901.

Genome annotation databases

EnsembliENSMUST00000067103; ENSMUSP00000063201; ENSMUSG00000023980. [Q9EQH4-1]
GeneIDi63856.
KEGGimmu:63856.
UCSCiuc008cvh.1. mouse. [Q9EQH4-1]
uc008cvj.1. mouse. [Q9EQH4-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTAF8_MOUSE
AccessioniPrimary (citable) accession number: Q9EQH4
Secondary accession number(s): Q3TSE4
, Q6PES8, Q8C382, Q8C664, Q8C7L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families