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Q9EQH3 (VPS35_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 35
Alternative name(s):
Maternal-embryonic 3
Vesicle protein sorting 35
Gene names
Name:Vps35
Synonyms:Mem3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length796 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) By similarity.

Subunit structure

Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts directly with VPS26A and VPS26B. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GOLPH3 By similarity. Ref.5

Subcellular location

Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.

Tissue specificity

Ubiquitous. Highly expressed in fat tissue, testis, brain, kidney, thymus, liver and pancreas, and at lower levels in heart, intestine and skeletal muscle. Detected in oocytes, pre-implantation embryos and at E6.5-E12.5.

Sequence similarities

Belongs to the VPS35 family.

Sequence caution

The sequence AAB18153.1 differs from that shown. Reason: Frameshift at several positions.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 796796Vacuolar protein sorting-associated protein 35
PRO_0000065897

Amino acid modifications

Modified residue71Phosphoserine By similarity
Modified residue7911Phosphotyrosine Ref.6 Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9EQH3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 2ABD338111D641CC

FASTA79691,713
        10         20         30         40         50         60 
MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DALKHASNML GELRTSMLSP 

        70         80         90        100        110        120 
KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY AGNIIPRLYL LITVGVVYVK 

       130        140        150        160        170        180 
SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR NYLLQCTRNI LPDEGEPTDE ETTGDISDSM 

       190        200        210        220        230        240 
DFVLLNFAEM NKLWVRMQHQ GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ 

       250        260        270        280        290        300 
IVLTGILEQV VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII 

       310        320        330        340        350        360 
ALIDRLALFA HREDGPGIPA EIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL QVSLINLAMK 

       370        380        390        400        410        420 
CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR LLKIPVDTYN NILTVLKLKH 

       430        440        450        460        470        480 
FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD 

       490        500        510        520        530        540 
PEDFADEQSL VGRFIHLLRS DDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA 

       550        560        570        580        590        600 
FRYKENSQMD DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE 

       610        620        630        640        650        660 
TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR TQCALAASKL 

       670        680        690        700        710        720 
LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL KKALKIANQC MDPSLQVQLF 

       730        740        750        760        770        780 
IEILNRYIYF YEKENDAVTI QVLNQLIQKI REDLPNLESS EETEQINKHF HNTLEHLRSR 

       790 
RESPESEGPI YEGLIL 

« Hide

References

« Hide 'large scale' references
[1]"Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3."
Hwang S.-Y., Benjamin L.E., Oh B., Rothstein J.L., Ackerman S.L., Beddington R.S.P., Solter D., Knowles B.B.
Mamm. Genome 7:586-590(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo and Embryonic carcinoma.
[2]"Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21."
Zhang P., Yu L., Gao J., Fu Q., Dai F., Zhao Y., Zheng L., Zhao S.
Genomics 70:253-257(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[4]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 91-107; 218-226; 297-305; 313-323; 372-382; 404-417 AND 574-582, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[5]"A novel mammalian retromer component, Vps26B."
Kerr M.C., Bennetts J.S., Simpson F., Thomas E.C., Flegg C., Gleeson P.A., Wicking C., Teasdale R.D.
Traffic 6:991-1001(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VPS26B.
[6]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47024 mRNA. Translation: AAB18153.1. Frameshift.
AF226323 mRNA. Translation: AAG40621.1.
BC005469 mRNA. Translation: AAH05469.1.
BC006637 mRNA. Translation: AAH06637.1.
RefSeqNP_075373.1. NM_022997.4.
UniGeneMm.296520.
Mm.391632.

3D structure databases

ProteinModelPortalQ9EQH3.
SMRQ9EQH3. Positions 483-780.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid211137. 5 interactions.
DIPDIP-32210N.
IntActQ9EQH3. 8 interactions.
MINTMINT-1870050.
STRING10090.ENSMUSP00000034131.

PTM databases

PhosphoSiteQ9EQH3.

Proteomic databases

PaxDbQ9EQH3.
PRIDEQ9EQH3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034131; ENSMUSP00000034131; ENSMUSG00000031696.
GeneID65114.
KEGGmmu:65114.
UCSCuc009mpo.1. mouse.

Organism-specific databases

CTD55737.
MGIMGI:1890467. Vps35.

Phylogenomic databases

eggNOGNOG252873.
GeneTreeENSGT00390000007315.
HOGENOMHOG000196946.
HOVERGENHBG054277.
InParanoidQ9EQH3.
OMAWADECEN.
OrthoDBEOG7TMZR5.
PhylomeDBQ9EQH3.
TreeFamTF105659.

Gene expression databases

ArrayExpressQ9EQH3.
BgeeQ9EQH3.
CleanExMM_VPS35.
GenevestigatorQ9EQH3.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR005378. Vps35.
[Graphical view]
PANTHERPTHR11099. PTHR11099. 1 hit.
PfamPF03635. Vps35. 1 hit.
[Graphical view]
PIRSFPIRSF009375. Retromer_Vps35. 1 hit.
SUPFAMSSF48371. SSF48371. 2 hits.
ProtoNetSearch...

Other

ChiTaRSVPS35. mouse.
NextBio320313.
PROQ9EQH3.
SOURCESearch...

Entry information

Entry nameVPS35_MOUSE
AccessionPrimary (citable) accession number: Q9EQH3
Secondary accession number(s): Q61123
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot