Q9EQH3 (VPS35_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 35

Alternative name(s):
Maternal-embryonic 3
Vesicle protein sorting 35

Gene names

Name:	Vps35
Synonyms:	Mem3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) By similarity.
Subunit structure	Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts directly with VPS26A and VPS26B. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GOLPH3 By similarity. Ref.5
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Ubiquitous. Highly expressed in fat tissue, testis, brain, kidney, thymus, liver and pancreas, and at lower levels in heart, intestine and skeletal muscle. Detected in oocytes, pre-implantation embryos and at E6.5-E12.5.
Sequence similarities	Belongs to the VPS35 family.
Sequence caution	The sequence AAB18153.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vacuolar protein processing Inferred from sequence alignment Ref.2. Source: MGI
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara endosome Inferred from electronic annotation. Source: Compara integral to membrane Inferred from sequence alignment Ref.2. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 796	796	Vacuolar protein sorting-associated protein 35		PRO_0000065897
Amino acid modifications
	Modified residue	7	1	Phosphoserine Ref.7
	Modified residue	791	1	Phosphotyrosine Ref.6 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9EQH3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2ABD338111D641CC	FASTA	796	91,713
	10 20 30 40 50 60 MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DALKHASNML GELRTSMLSP 70 80 90 100 110 120 KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY AGNIIPRLYL LITVGVVYVK 130 140 150 160 170 180 SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR NYLLQCTRNI LPDEGEPTDE ETTGDISDSM 190 200 210 220 230 240 DFVLLNFAEM NKLWVRMQHQ GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ 250 260 270 280 290 300 IVLTGILEQV VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII 310 320 330 340 350 360 ALIDRLALFA HREDGPGIPA EIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL QVSLINLAMK 370 380 390 400 410 420 CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR LLKIPVDTYN NILTVLKLKH 430 440 450 460 470 480 FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD 490 500 510 520 530 540 PEDFADEQSL VGRFIHLLRS DDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA 550 560 570 580 590 600 FRYKENSQMD DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE 610 620 630 640 650 660 TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR TQCALAASKL 670 680 690 700 710 720 LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL KKALKIANQC MDPSLQVQLF 730 740 750 760 770 780 IEILNRYIYF YEKENDAVTI QVLNQLIQKI REDLPNLESS EETEQINKHF HNTLEHLRSR 790 RESPESEGPI YEGLIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3." Hwang S.-Y., Benjamin L.E., Oh B., Rothstein J.L., Ackerman S.L., Beddington R.S.P., Solter D., Knowles B.B. Mamm. Genome 7:586-590(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo and Embryonic carcinoma.
[2]	"Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21." Zhang P., Yu L., Gao J., Fu Q., Dai F., Zhao Y., Zheng L., Zhao S. Genomics 70:253-257(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 91-107; 218-226; 297-305; 313-323; 372-382; 404-417 AND 574-582, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"A novel mammalian retromer component, Vps26B." Kerr M.C., Bennetts J.S., Simpson F., Thomas E.C., Flegg C., Gleeson P.A., Wicking C., Teasdale R.D. Traffic 6:991-1001(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VPS26B.
[6]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, MASS SPECTROMETRY. Tissue: Mast cell.
[7]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY. Tissue: Brain cortex.
[8]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U47024 mRNA. Translation: AAB18153.1. Frameshift. AF226323 mRNA. Translation: AAG40621.1. BC005469 mRNA. Translation: AAH05469.1. BC006637 mRNA. Translation: AAH06637.1.
IPI	IPI00111181.
RefSeq	NP_075373.1. NM_022997.4.
UniGene	Mm.296520.
3D structure databases
ProteinModelPortal	Q9EQH3.
SMR	Q9EQH3. Positions 483-780.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-32210N.
IntAct	Q9EQH3. 2 interactions.
STRING	10090.ENSMUSP00000034131.
PTM databases
PhosphoSite	Q9EQH3.
Proteomic databases
PaxDb	Q9EQH3.
PRIDE	Q9EQH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034131; ENSMUSP00000034131; ENSMUSG00000031696.
GeneID	65114.
KEGG	mmu:65114.
Organism-specific databases
CTD	55737.
MGI	MGI:1890467. Vps35.
Phylogenomic databases
eggNOG	NOG252873.
GeneTree	ENSGT00390000007315.
HOGENOM	HOG000196946.
HOVERGEN	HBG054277.
InParanoid	Q9EQH3.
OMA	VQLFEVF.
OrthoDB	EOG4HT8RK.
Gene expression databases
ArrayExpress	Q9EQH3.
Bgee	Q9EQH3.
CleanEx	MM_VPS35.
Genevestigator	Q9EQH3.
GermOnline	ENSMUSG00000031696. Mus musculus.
Family and domain databases
InterPro	IPR005378. VPS35. [Graphical view]
PANTHER	PTHR11099. PTHR11099. 1 hit.
Pfam	PF03635. Vps35. 1 hit. [Graphical view]
PIRSF	PIRSF009375. Retromer_Vps35. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	VPS35. mouse.
NextBio	320313.
SOURCE	Search...

Entry information

Entry name

VPS35_MOUSE

Accession

Primary (citable) accession number: Q9EQH3
Secondary accession number(s): Q61123

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program