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Q9EQH3

- VPS35_MOUSE

UniProt

Q9EQH3 - VPS35_MOUSE

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Protein

Vacuolar protein sorting-associated protein 35

Gene

Vps35

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of FAM21 and mediates the association of the CSC with the WASH complex (By similarity).By similarity

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. retrograde transport, endosome to Golgi Source: InterPro
  3. vacuolar protein processing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_213918. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 35
Alternative name(s):
Maternal-embryonic 3
Vesicle protein sorting 35
Gene namesi
Name:Vps35
Synonyms:Mem3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1890467. Vps35.

Subcellular locationi

Cytoplasm By similarity. Membrane By similarity; Peripheral membrane protein By similarity. Endosome By similarity. Early endosome Curated. Late endosome Curated

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. endosome Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. integral component of membrane Source: MGI
  5. lysosomal membrane Source: Ensembl
  6. neuronal postsynaptic density Source: MGI
  7. retromer complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 796796Vacuolar protein sorting-associated protein 35PRO_0000065897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineBy similarity
Modified residuei791 – 7911Phosphotyrosine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9EQH3.
PaxDbiQ9EQH3.
PRIDEiQ9EQH3.

PTM databases

PhosphoSiteiQ9EQH3.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in fat tissue, testis, brain, kidney, thymus, liver and pancreas, and at lower levels in heart, intestine and skeletal muscle. Detected in oocytes, pre-implantation embryos and at E6.5-E12.5.

Gene expression databases

BgeeiQ9EQH3.
CleanExiMM_VPS35.
ExpressionAtlasiQ9EQH3. baseline and differential.
GenevestigatoriQ9EQH3.

Interactioni

Subunit structurei

Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS) formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35. The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform (PubMed:21040701, PubMed:20875039, PubMed:21920005). The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described retromer complex (also called SNX-BAR retromer) is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the affinity between the respective CSC and SNX-BAR subcomplexes is low. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex (Probable). Interacts with VPS26A, VPS29, VPS26B and LRRK2 (PubMed:16190980, PubMed:21040701, PubMed:20875039, PubMed:21920005, PubMed:23395371). Interacts with SNX1, SNX2, IGF2R, SNX3, GOLPH3, SLC11A2, FAM21, FKBP15, WASH1, EHD1. Interacts with MAGEL2; leading to recruitment of the TRIM27:MAGEL2 E3 ubiquitin ligase complex retromer-containing endosomes (By similarity).By similarity5 Publications

Protein-protein interaction databases

BioGridi211137. 6 interactions.
DIPiDIP-32210N.
IntActiQ9EQH3. 12 interactions.
MINTiMINT-1870050.
STRINGi10090.ENSMUSP00000034131.

Structurei

3D structure databases

ProteinModelPortaliQ9EQH3.
SMRiQ9EQH3. Positions 483-780.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 4420Interaction with SNX3By similarityAdd
BLAST
Regioni205 – 21511Interaction with SNX3By similarityAdd
BLAST
Regioni438 – 796359Interaction with SLC11A2By similarityAdd
BLAST
Regioni500 – 693194Interaction with IGF2R cytoplasmic domainBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the VPS35 family.Curated

Phylogenomic databases

eggNOGiNOG252873.
GeneTreeiENSGT00390000007315.
HOGENOMiHOG000196946.
HOVERGENiHBG054277.
InParanoidiQ9EQH3.
KOiK18468.
OMAiMECIIQX.
OrthoDBiEOG7TMZR5.
PhylomeDBiQ9EQH3.
TreeFamiTF105659.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR005378. Vps35.
[Graphical view]
PANTHERiPTHR11099. PTHR11099. 1 hit.
PfamiPF03635. Vps35. 1 hit.
[Graphical view]
PIRSFiPIRSF009375. Retromer_Vps35. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9EQH3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DALKHASNML
60 70 80 90 100
GELRTSMLSP KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY
110 120 130 140 150
AGNIIPRLYL LITVGVVYVK SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR
160 170 180 190 200
NYLLQCTRNI LPDEGEPTDE ETTGDISDSM DFVLLNFAEM NKLWVRMQHQ
210 220 230 240 250
GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ IVLTGILEQV
260 270 280 290 300
VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII
310 320 330 340 350
ALIDRLALFA HREDGPGIPA EIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL
360 370 380 390 400
QVSLINLAMK CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR
410 420 430 440 450
LLKIPVDTYN NILTVLKLKH FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE
460 470 480 490 500
IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD PEDFADEQSL VGRFIHLLRS
510 520 530 540 550
DDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA FRYKENSQMD
560 570 580 590 600
DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE
610 620 630 640 650
TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR
660 670 680 690 700
TQCALAASKL LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL
710 720 730 740 750
KKALKIANQC MDPSLQVQLF IEILNRYIYF YEKENDAVTI QVLNQLIQKI
760 770 780 790
REDLPNLESS EETEQINKHF HNTLEHLRSR RESPESEGPI YEGLIL
Length:796
Mass (Da):91,713
Last modified:March 1, 2001 - v1
Checksum:i2ABD338111D641CC
GO

Sequence cautioni

The sequence AAB18153.1 differs from that shown. Reason: Frameshift at several positions. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47024 mRNA. Translation: AAB18153.1. Frameshift.
AF226323 mRNA. Translation: AAG40621.1.
BC005469 mRNA. Translation: AAH05469.1.
BC006637 mRNA. Translation: AAH06637.1.
CCDSiCCDS40421.1.
RefSeqiNP_075373.1. NM_022997.4.
UniGeneiMm.296520.
Mm.391632.

Genome annotation databases

EnsembliENSMUST00000034131; ENSMUSP00000034131; ENSMUSG00000031696.
GeneIDi65114.
KEGGimmu:65114.
UCSCiuc009mpo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47024 mRNA. Translation: AAB18153.1 . Frameshift.
AF226323 mRNA. Translation: AAG40621.1 .
BC005469 mRNA. Translation: AAH05469.1 .
BC006637 mRNA. Translation: AAH06637.1 .
CCDSi CCDS40421.1.
RefSeqi NP_075373.1. NM_022997.4.
UniGenei Mm.296520.
Mm.391632.

3D structure databases

ProteinModelPortali Q9EQH3.
SMRi Q9EQH3. Positions 483-780.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211137. 6 interactions.
DIPi DIP-32210N.
IntActi Q9EQH3. 12 interactions.
MINTi MINT-1870050.
STRINGi 10090.ENSMUSP00000034131.

PTM databases

PhosphoSitei Q9EQH3.

Proteomic databases

MaxQBi Q9EQH3.
PaxDbi Q9EQH3.
PRIDEi Q9EQH3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034131 ; ENSMUSP00000034131 ; ENSMUSG00000031696 .
GeneIDi 65114.
KEGGi mmu:65114.
UCSCi uc009mpo.1. mouse.

Organism-specific databases

CTDi 55737.
MGIi MGI:1890467. Vps35.

Phylogenomic databases

eggNOGi NOG252873.
GeneTreei ENSGT00390000007315.
HOGENOMi HOG000196946.
HOVERGENi HBG054277.
InParanoidi Q9EQH3.
KOi K18468.
OMAi MECIIQX.
OrthoDBi EOG7TMZR5.
PhylomeDBi Q9EQH3.
TreeFami TF105659.

Enzyme and pathway databases

Reactomei REACT_213918. WNT ligand biogenesis and trafficking.

Miscellaneous databases

ChiTaRSi Vps35. mouse.
NextBioi 320313.
PROi Q9EQH3.
SOURCEi Search...

Gene expression databases

Bgeei Q9EQH3.
CleanExi MM_VPS35.
ExpressionAtlasi Q9EQH3. baseline and differential.
Genevestigatori Q9EQH3.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR005378. Vps35.
[Graphical view ]
PANTHERi PTHR11099. PTHR11099. 1 hit.
Pfami PF03635. Vps35. 1 hit.
[Graphical view ]
PIRSFi PIRSF009375. Retromer_Vps35. 1 hit.
SUPFAMi SSF48371. SSF48371. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3."
    Hwang S.-Y., Benjamin L.E., Oh B., Rothstein J.L., Ackerman S.L., Beddington R.S.P., Solter D., Knowles B.B.
    Mamm. Genome 7:586-590(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo and Embryonic carcinoma.
  2. "Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21."
    Zhang P., Yu L., Gao J., Fu Q., Dai F., Zhao Y., Zheng L., Zhao S.
    Genomics 70:253-257(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 91-107; 218-226; 297-305; 313-323; 372-382; 404-417 AND 574-582, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  5. Cited for: INTERACTION WITH VPS26B.
  6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. "Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking."
    Kim E., Lee Y., Lee H.J., Kim J.S., Song B.S., Huh J.W., Lee S.R., Kim S.U., Kim S.H., Hong Y., Shim I., Chang K.T.
    Biochem. Biophys. Res. Commun. 403:167-171(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  9. "Assembly and solution structure of the core retromer protein complex."
    Norwood S.J., Shaw D.J., Cowieson N.P., Owen D.J., Teasdale R.D., Collins B.M.
    Traffic 12:56-71(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  10. "Vps26A and Vps26B subunits define distinct retromer complexes."
    Bugarcic A., Zhe Y., Kerr M.C., Griffin J., Collins B.M., Teasdale R.D.
    Traffic 12:1759-1773(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  11. "RAB7L1 interacts with LRRK2 to modify intraneuronal protein sorting and Parkinson's disease risk."
    MacLeod D.A., Rhinn H., Kuwahara T., Zolin A., Di Paolo G., McCabe B.D., MacCabe B.D., Marder K.S., Honig L.S., Clark L.N., Small S.A., Abeliovich A.
    Neuron 77:425-439(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LRRK2.

Entry informationi

Entry nameiVPS35_MOUSE
AccessioniPrimary (citable) accession number: Q9EQH3
Secondary accession number(s): Q61123
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.