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Q9EQH3

- VPS35_MOUSE

UniProt

Q9EQH3 - VPS35_MOUSE

Protein

Vacuolar protein sorting-associated protein 35

Gene

Vps35

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. retrograde transport, endosome to Golgi Source: InterPro
    3. vacuolar protein processing Source: MGI

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_213918. WNT ligand biogenesis and trafficking.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 35
    Alternative name(s):
    Maternal-embryonic 3
    Vesicle protein sorting 35
    Gene namesi
    Name:Vps35
    Synonyms:Mem3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1890467. Vps35.

    Subcellular locationi

    Cytoplasm By similarity. Membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. endosome Source: Ensembl
    3. integral component of membrane Source: MGI
    4. lysosomal membrane Source: Ensembl
    5. retromer complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 796796Vacuolar protein sorting-associated protein 35PRO_0000065897Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei7 – 71PhosphoserineBy similarity
    Modified residuei791 – 7911Phosphotyrosine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9EQH3.
    PaxDbiQ9EQH3.
    PRIDEiQ9EQH3.

    PTM databases

    PhosphoSiteiQ9EQH3.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highly expressed in fat tissue, testis, brain, kidney, thymus, liver and pancreas, and at lower levels in heart, intestine and skeletal muscle. Detected in oocytes, pre-implantation embryos and at E6.5-E12.5.

    Gene expression databases

    ArrayExpressiQ9EQH3.
    BgeeiQ9EQH3.
    CleanExiMM_VPS35.
    GenevestigatoriQ9EQH3.

    Interactioni

    Subunit structurei

    Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts directly with VPS26A and VPS26B. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GOLPH3. Interacts with MAGEL2; leading to recruitment of the TRIM27:MAGEL2 E3 ubiquitin ligase complex retromer-containing endosomes By similarity. Interacts with LRRK2.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi211137. 6 interactions.
    DIPiDIP-32210N.
    IntActiQ9EQH3. 8 interactions.
    MINTiMINT-1870050.
    STRINGi10090.ENSMUSP00000034131.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EQH3.
    SMRiQ9EQH3. Positions 483-780.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the VPS35 family.Curated

    Phylogenomic databases

    eggNOGiNOG252873.
    GeneTreeiENSGT00390000007315.
    HOGENOMiHOG000196946.
    HOVERGENiHBG054277.
    InParanoidiQ9EQH3.
    OMAiGEFRTSM.
    OrthoDBiEOG7TMZR5.
    PhylomeDBiQ9EQH3.
    TreeFamiTF105659.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR005378. Vps35.
    [Graphical view]
    PANTHERiPTHR11099. PTHR11099. 1 hit.
    PfamiPF03635. Vps35. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009375. Retromer_Vps35. 1 hit.
    SUPFAMiSSF48371. SSF48371. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9EQH3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DALKHASNML    50
    GELRTSMLSP KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY 100
    AGNIIPRLYL LITVGVVYVK SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR 150
    NYLLQCTRNI LPDEGEPTDE ETTGDISDSM DFVLLNFAEM NKLWVRMQHQ 200
    GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ IVLTGILEQV 250
    VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII 300
    ALIDRLALFA HREDGPGIPA EIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL 350
    QVSLINLAMK CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR 400
    LLKIPVDTYN NILTVLKLKH FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE 450
    IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD PEDFADEQSL VGRFIHLLRS 500
    DDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA FRYKENSQMD 550
    DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE 600
    TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR 650
    TQCALAASKL LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL 700
    KKALKIANQC MDPSLQVQLF IEILNRYIYF YEKENDAVTI QVLNQLIQKI 750
    REDLPNLESS EETEQINKHF HNTLEHLRSR RESPESEGPI YEGLIL 796
    Length:796
    Mass (Da):91,713
    Last modified:March 1, 2001 - v1
    Checksum:i2ABD338111D641CC
    GO

    Sequence cautioni

    The sequence AAB18153.1 differs from that shown. Reason: Frameshift at several positions.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47024 mRNA. Translation: AAB18153.1. Frameshift.
    AF226323 mRNA. Translation: AAG40621.1.
    BC005469 mRNA. Translation: AAH05469.1.
    BC006637 mRNA. Translation: AAH06637.1.
    CCDSiCCDS40421.1.
    RefSeqiNP_075373.1. NM_022997.4.
    UniGeneiMm.296520.
    Mm.391632.

    Genome annotation databases

    EnsembliENSMUST00000034131; ENSMUSP00000034131; ENSMUSG00000031696.
    GeneIDi65114.
    KEGGimmu:65114.
    UCSCiuc009mpo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47024 mRNA. Translation: AAB18153.1 . Frameshift.
    AF226323 mRNA. Translation: AAG40621.1 .
    BC005469 mRNA. Translation: AAH05469.1 .
    BC006637 mRNA. Translation: AAH06637.1 .
    CCDSi CCDS40421.1.
    RefSeqi NP_075373.1. NM_022997.4.
    UniGenei Mm.296520.
    Mm.391632.

    3D structure databases

    ProteinModelPortali Q9EQH3.
    SMRi Q9EQH3. Positions 483-780.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211137. 6 interactions.
    DIPi DIP-32210N.
    IntActi Q9EQH3. 8 interactions.
    MINTi MINT-1870050.
    STRINGi 10090.ENSMUSP00000034131.

    PTM databases

    PhosphoSitei Q9EQH3.

    Proteomic databases

    MaxQBi Q9EQH3.
    PaxDbi Q9EQH3.
    PRIDEi Q9EQH3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034131 ; ENSMUSP00000034131 ; ENSMUSG00000031696 .
    GeneIDi 65114.
    KEGGi mmu:65114.
    UCSCi uc009mpo.1. mouse.

    Organism-specific databases

    CTDi 55737.
    MGIi MGI:1890467. Vps35.

    Phylogenomic databases

    eggNOGi NOG252873.
    GeneTreei ENSGT00390000007315.
    HOGENOMi HOG000196946.
    HOVERGENi HBG054277.
    InParanoidi Q9EQH3.
    OMAi GEFRTSM.
    OrthoDBi EOG7TMZR5.
    PhylomeDBi Q9EQH3.
    TreeFami TF105659.

    Enzyme and pathway databases

    Reactomei REACT_213918. WNT ligand biogenesis and trafficking.

    Miscellaneous databases

    ChiTaRSi VPS35. mouse.
    NextBioi 320313.
    PROi Q9EQH3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9EQH3.
    Bgeei Q9EQH3.
    CleanExi MM_VPS35.
    Genevestigatori Q9EQH3.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR005378. Vps35.
    [Graphical view ]
    PANTHERi PTHR11099. PTHR11099. 1 hit.
    Pfami PF03635. Vps35. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009375. Retromer_Vps35. 1 hit.
    SUPFAMi SSF48371. SSF48371. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3."
      Hwang S.-Y., Benjamin L.E., Oh B., Rothstein J.L., Ackerman S.L., Beddington R.S.P., Solter D., Knowles B.B.
      Mamm. Genome 7:586-590(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo and Embryonic carcinoma.
    2. "Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21."
      Zhang P., Yu L., Gao J., Fu Q., Dai F., Zhao Y., Zheng L., Zhao S.
      Genomics 70:253-257(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    4. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 91-107; 218-226; 297-305; 313-323; 372-382; 404-417 AND 574-582, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    5. Cited for: INTERACTION WITH VPS26B.
    6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    8. "RAB7L1 interacts with LRRK2 to modify intraneuronal protein sorting and Parkinson's disease risk."
      MacLeod D.A., Rhinn H., Kuwahara T., Zolin A., Di Paolo G., McCabe B.D., MacCabe B.D., Marder K.S., Honig L.S., Clark L.N., Small S.A., Abeliovich A.
      Neuron 77:425-439(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LRRK2.

    Entry informationi

    Entry nameiVPS35_MOUSE
    AccessioniPrimary (citable) accession number: Q9EQH3
    Secondary accession number(s): Q61123
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3