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Protein

GRB2-associated-binding protein 2

Gene

Gab2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-associated-binding protein 2
Alternative name(s):
GRB2-associated binder 2
Growth factor receptor bound protein 2-associated protein 2
Gene namesi
Name:Gab2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621367 Gab2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000502871 – 665GRB2-associated-binding protein 2Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineBy similarity1
Modified residuei135PhosphoserineBy similarity1
Modified residuei142PhosphoserineBy similarity1
Modified residuei143PhosphoserineBy similarity1
Modified residuei149PhosphoserineBy similarity1
Modified residuei150PhosphoserineBy similarity1
Modified residuei160PhosphoserineBy similarity1
Modified residuei165PhosphoserineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Modified residuei220PhosphoserineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei262PhosphothreonineBy similarity1
Modified residuei263PhosphotyrosineBy similarity1
Modified residuei275PhosphothreonineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Modified residuei282PhosphoserineBy similarity1
Modified residuei284PhosphothreonineBy similarity1
Modified residuei290PhosphotyrosineBy similarity1
Modified residuei328PhosphothreonineBy similarity1
Modified residuei365PhosphoserineBy similarity1
Modified residuei382PhosphothreonineBy similarity1
Modified residuei388PhosphothreonineBy similarity1
Modified residuei402PhosphoserineBy similarity1
Modified residuei405PhosphothreonineBy similarity1
Modified residuei420PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei441PhosphotyrosineBy similarity1
Modified residuei532PhosphoserineBy similarity1
Modified residuei612PhosphoserineBy similarity1
Modified residuei632PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling (By similarity). Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity).By similarity
Dephosphorylated by PTPN11.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9EQH1
PRIDEiQ9EQH1

PTM databases

iPTMnetiQ9EQH1
PhosphoSitePlusiQ9EQH1

Interactioni

Subunit structurei

Interacts with HCK. Interacts with SHC1; may mediate interaction with receptors (By similarity). Interacts with SYK (By similarity). Interacts with PI-3 kinase (By similarity). Interacts with GRB2 (via SH3 2 domain) (By similarity). Interacts (phosphorylated) with PTPN11 (By similarity). Interacts with TNFRSF11A (via cytoplasmic domain) (By similarity). Interacts (phosphorylated) with 14-3-3 family proteins SFN, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ; prevents interaction with GRB2 and attenuates GAB2 signaling (By similarity).By similarity

GO - Molecular functioni

  • protein domain specific binding Source: RGD
  • protein phosphatase binding Source: RGD
  • transmembrane receptor protein tyrosine kinase adaptor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi249977, 1 interactor
CORUMiQ9EQH1
IntActiQ9EQH1, 1 interactor
MINTiQ9EQH1
STRINGi10116.ENSRNOP00000016361

Structurei

3D structure databases

ProteinModelPortaliQ9EQH1
SMRiQ9EQH1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 119PHPROSITE-ProRule annotationAdd BLAST112

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi348 – 355SH3-bindingBy similarity8
Motifi499 – 508SH3-bindingBy similarity10

Domaini

The SH3-binding motifs mediate interaction with SHC1 and GRB2.By similarity
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.By similarity

Sequence similaritiesi

Belongs to the GAB family.Curated

Phylogenomic databases

HOGENOMiHOG000236270
HOVERGENiHBG051685
InParanoidiQ9EQH1
KOiK08091
PhylomeDBiQ9EQH1

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9EQH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGGGDDVV CTGWLRKSPP EKKLRRYAWK KRWFILRSGR MSGDPDVLEY
60 70 80 90 100
YKNEHSKKPL RIINLNFCEQ VDAGLTFNKK ELQDSFVFDI KTSERTFYLV
110 120 130 140 150
AETEADMNKW VQSICQICGF NQAEESTDSL RNLSSASHGP RSSPAEFSSS
160 170 180 190 200
QHLLRERKSS APSHSSQPTL FTFEPPMTSH MQPALSTSAP QEYLYLHQCI
210 220 230 240 250
SRRTENSRSA SFSQGTRQKS DTAVQKLAQS NGHCINGVSN QVHGFYSLPK
260 270 280 290 300
PSRHNTEFKD STYDLPRSLA SHGHTKSSLT GSETDNEDVY TFKMPSNTLC
310 320 330 340 350
REFGDLLVDN MDVPTTPLSA YQIPRTFTLD KNHNAMTVAT SGDSAIAPPP
360 370 380 390 400
RPPKPSQAET PRWGSPQQKP PIGENSRSVA ATIPRRNTLP AMDNSRLHRA
410 420 430 440 450
SSCETYEYPT RGSGESASWS AESPGKTAVG RSDSASSDEN YVPMNPGSST
460 470 480 490 500
LLAMERAGDN SQSAYIPMGP GPHHFDPLGY PSTALPIHRG PSRGSEIQPP
510 520 530 540 550
PVNRNLKPDR KAKPTPLDLR NNTVIDELPF KSPVTKSWSR INHTFNSSSS
560 570 580 590 600
QYCRPISTQS ITSTDSGDSE ENYVPMQNPV SASPVPSGTN SPAPRKSTGS
610 620 630 640 650
VDYLALDFQP GSPSPHRKPS TSSVTSDEKV DYVQVDKEKT QALQNTMQEW
660
TDVRQSSEPS KGAKL
Length:665
Mass (Da):73,328
Last modified:October 1, 2001 - v2
Checksum:iBEB170B69406063E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230367 mRNA Translation: AAG44268.2
RefSeqiNP_445869.1, NM_053417.1
UniGeneiRn.211990

Genome annotation databases

GeneIDi84477
KEGGirno:84477
UCSCiRGD:621367 rat

Similar proteinsi

Entry informationi

Entry nameiGAB2_RAT
AccessioniPrimary (citable) accession number: Q9EQH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2001
Last modified: April 25, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health