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Q9EQH1 (GAB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GRB2-associated-binding protein 2
Alternative name(s):
GRB2-associated binder 2
Growth factor receptor bound protein 2-associated protein 2
Gene names
Name:Gab2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length665 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis By similarity.

Subunit structure

Interacts with HCK. Interacts with SHC1; may mediate interaction with receptors By similarity. Interacts with SYK By similarity. Interacts with PI-3 kinase By similarity. Interacts with GRB2 (via SH3 2 domain) By similarity. Interacts (phosphorylated) with PTPN11 By similarity. Interacts with TNFRSF11A (via cytoplasmic domain) By similarity. Interacts (phosphorylated) with 14-3-3 family proteins SFN, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ; prevents interaction with GRB2 and attenuates GAB2 signaling By similarity.

Subcellular location

Cytoplasm By similarity. Cell membrane By similarity.

Domain

The SH3-binding motifs mediate interaction with SHC1 and GRB2 By similarity.

The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding By similarity.

Post-translational modification

Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling By similarity. Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R By similarity. Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL By similarity.

Dephosphorylated by PTPN11 By similarity.

Sequence similarities

Belongs to the GAB family.

Contains 1 PH domain.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasm
Membrane
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular signal transduction

Inferred from direct assay PubMed 10973965. Source: RGD

osteoclast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

phosphatidylinositol-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of mast cell degranulation

Inferred from sequence or structural similarity. Source: UniProtKB

transmembrane receptor protein tyrosine kinase signaling pathway

Inferred from sequence or structural similarity. Source: GOC

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

protein complex

Inferred from direct assay PubMed 10973965. Source: RGD

   Molecular_functionphosphatidylinositol-3,4,5-trisphosphate binding

Inferred from sequence or structural similarity. Source: UniProtKB

phosphatidylinositol-3,4-bisphosphate binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein domain specific binding

Inferred from physical interaction PubMed 10973965. Source: RGD

transmembrane receptor protein tyrosine kinase adaptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 665665GRB2-associated-binding protein 2
PRO_0000050287

Regions

Domain8 – 119112PH
Motif348 – 3558SH3-binding By similarity
Motif499 – 50810SH3-binding By similarity

Amino acid modifications

Modified residue1351Phosphoserine By similarity
Modified residue1421Phosphoserine By similarity
Modified residue1431Phosphoserine By similarity
Modified residue1491Phosphoserine By similarity
Modified residue1501Phosphoserine By similarity
Modified residue1601Phosphoserine By similarity
Modified residue1651Phosphoserine By similarity
Modified residue2111Phosphoserine By similarity
Modified residue2201Phosphoserine By similarity
Modified residue2611Phosphoserine By similarity
Modified residue2621Phosphothreonine By similarity
Modified residue2631Phosphotyrosine By similarity
Modified residue2751Phosphothreonine By similarity
Modified residue2781Phosphoserine By similarity
Modified residue2841Phosphothreonine By similarity
Modified residue2901Phosphotyrosine By similarity
Modified residue3281Phosphothreonine By similarity
Modified residue3821Phosphothreonine By similarity
Modified residue3881Phosphothreonine By similarity
Modified residue4021Phosphoserine By similarity
Modified residue4411Phosphotyrosine By similarity
Modified residue5321Phosphoserine By similarity
Modified residue6121Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9EQH1 [UniParc].

Last modified October 1, 2001. Version 2.
Checksum: BEB170B69406063E

FASTA66573,328
        10         20         30         40         50         60 
MSGGGGDDVV CTGWLRKSPP EKKLRRYAWK KRWFILRSGR MSGDPDVLEY YKNEHSKKPL 

        70         80         90        100        110        120 
RIINLNFCEQ VDAGLTFNKK ELQDSFVFDI KTSERTFYLV AETEADMNKW VQSICQICGF 

       130        140        150        160        170        180 
NQAEESTDSL RNLSSASHGP RSSPAEFSSS QHLLRERKSS APSHSSQPTL FTFEPPMTSH 

       190        200        210        220        230        240 
MQPALSTSAP QEYLYLHQCI SRRTENSRSA SFSQGTRQKS DTAVQKLAQS NGHCINGVSN 

       250        260        270        280        290        300 
QVHGFYSLPK PSRHNTEFKD STYDLPRSLA SHGHTKSSLT GSETDNEDVY TFKMPSNTLC 

       310        320        330        340        350        360 
REFGDLLVDN MDVPTTPLSA YQIPRTFTLD KNHNAMTVAT SGDSAIAPPP RPPKPSQAET 

       370        380        390        400        410        420 
PRWGSPQQKP PIGENSRSVA ATIPRRNTLP AMDNSRLHRA SSCETYEYPT RGSGESASWS 

       430        440        450        460        470        480 
AESPGKTAVG RSDSASSDEN YVPMNPGSST LLAMERAGDN SQSAYIPMGP GPHHFDPLGY 

       490        500        510        520        530        540 
PSTALPIHRG PSRGSEIQPP PVNRNLKPDR KAKPTPLDLR NNTVIDELPF KSPVTKSWSR 

       550        560        570        580        590        600 
INHTFNSSSS QYCRPISTQS ITSTDSGDSE ENYVPMQNPV SASPVPSGTN SPAPRKSTGS 

       610        620        630        640        650        660 
VDYLALDFQP GSPSPHRKPS TSSVTSDEKV DYVQVDKEKT QALQNTMQEW TDVRQSSEPS 


KGAKL 

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References

[1]"Identification of Gab2 as the major molecule responsible for EGF-induced PI3-kinase activation and DNA synthesis in rat hepatocytes."
Kong M., Mounier C., Wu J., Posner B.I.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF230367 mRNA. Translation: AAG44268.2.
RefSeqNP_445869.1. NM_053417.1.
UniGeneRn.211990.

3D structure databases

ProteinModelPortalQ9EQH1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249977. 1 interaction.
IntActQ9EQH1. 1 interaction.
MINTMINT-7138417.
STRING10116.ENSRNOP00000016361.

PTM databases

PhosphoSiteQ9EQH1.

Proteomic databases

PaxDbQ9EQH1.
PRIDEQ9EQH1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID84477.
KEGGrno:84477.
UCSCRGD:621367. rat.

Organism-specific databases

CTD9846.
RGD621367. Gab2.

Phylogenomic databases

eggNOGNOG83177.
HOGENOMHOG000236270.
HOVERGENHBG051685.
InParanoidQ9EQH1.
KOK08091.
PhylomeDBQ9EQH1.

Gene expression databases

GenevestigatorQ9EQH1.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio616982.
PROQ9EQH1.

Entry information

Entry nameGAB2_RAT
AccessionPrimary (citable) accession number: Q9EQH1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2001
Last modified: April 16, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families