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Protein

Collagen type IV alpha-3-binding protein

Gene

Col4a3bp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei472CeramideBy similarity1
Binding sitei493CeramideBy similarity1
Binding sitei530CeramideBy similarity1
Binding sitei579CeramideBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell morphogenesis Source: MGI
  • cell proliferation Source: MGI
  • ceramide metabolic process Source: MGI
  • endoplasmic reticulum organization Source: MGI
  • ER to Golgi ceramide transport Source: UniProtKB
  • heart morphogenesis Source: MGI
  • in utero embryonic development Source: MGI
  • lipid homeostasis Source: MGI
  • mitochondrion morphogenesis Source: MGI
  • muscle contraction Source: MGI
  • response to endoplasmic reticulum stress Source: MGI
  • signal transduction Source: MGI

Keywordsi

Biological processLipid transport, Transport
LigandLipid-binding

Enzyme and pathway databases

BRENDAi2.7.11.9. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen type IV alpha-3-binding protein
Alternative name(s):
Ceramide transfer protein
Goodpasture antigen-binding protein
Short name:
GPBP
START domain-containing protein 11
Short name:
StARD11
StAR-related lipid transfer protein 11
Gene namesi
Name:Col4a3bp
Synonyms:Stard11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1915268. Col4a3bp.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002206661 – 624Collagen type IV alpha-3-binding proteinAdd BLAST624

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineBy similarity1
Modified residuei132PhosphoserineCombined sources1
Modified residuei135PhosphoserineCombined sources1
Modified residuei315PhosphoserineBy similarity1
Modified residuei372PhosphotyrosineBy similarity1
Modified residuei373PhosphoserineBy similarity1
Modified residuei377PhosphoserineCombined sources1
Modified residuei380PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. Inactivated by hyperphosphorylation of serine residues by CSNK1G2/CK1 that triggers dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9EQG9.
PeptideAtlasiQ9EQG9.
PRIDEiQ9EQG9.

PTM databases

iPTMnetiQ9EQG9.
PhosphoSitePlusiQ9EQG9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021669.
GenevisibleiQ9EQG9. MM.

Interactioni

Subunit structurei

Interacts with COL4A3. Interacts with VAPA and VAPB. Interaction with VAPB is less efficient than with VAPA.By similarity

Protein-protein interaction databases

IntActiQ9EQG9. 1 interactor.
MINTiMINT-4089513.
STRINGi10090.ENSMUSP00000076856.

Structurei

3D structure databases

ProteinModelPortaliQ9EQG9.
SMRiQ9EQG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 117PHPROSITE-ProRule annotationAdd BLAST95
Domaini389 – 618STARTPROSITE-ProRule annotationAdd BLAST230

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili263 – 303Sequence analysisAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi321 – 327FFATBy similarity7

Domaini

The START domain recognizes ceramides and diacylglycerol lipids, interacts with membranes, and mediates the intermembrane transfer of ceramides and diacylglycerol lipids.By similarity
The PH domain targets the Golgi apparatus.By similarity
The FFAT motif is required for interaction with VAPA and VAPB.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1739. Eukaryota.
ENOG410XQTB. LUCA.
GeneTreeiENSGT00650000093230.
HOVERGENiHBG050753.
InParanoidiQ9EQG9.
KOiK08283.
OMAiSHALWPE.
OrthoDBiEOG091G063Z.
PhylomeDBiQ9EQG9.
TreeFamiTF106160.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.530.20. 1 hit.
InterProiView protein in InterPro
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
PfamiView protein in Pfam
PF00169. PH. 1 hit.
PF01852. START. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00234. START. 1 hit.
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiView protein in PROSITE
PS50003. PH_DOMAIN. 1 hit.
PS50848. START. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EQG9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDNQSWNSS GSEEDPETES GPPVERCGVL SKWTNYIHGW QDRWVVLKNN
60 70 80 90 100
TLSYYKSEDE TEYGCRGSIC LSKAVITPHD FDECRFDISV NDSVWYLRAQ
110 120 130 140 150
DPEHRQQWVD AIEQHKTESG YGSESSLRRH GSMVSLVSGA SGYSATSTSS
160 170 180 190 200
FKKGHSLREK LAEMETFRDI LCRQVDTLQK YFDVCADAVS KDELQRDKVV
210 220 230 240 250
EDDEDDFPTT RSDGDFLHNT NGNKEKLFPH VTPKGINGID FKGEAITFKA
260 270 280 290 300
TTAGILATLS HCIELMVKRE ESWQKRHDRE VEKRRRVEEA YKNVMEELKK
310 320 330 340 350
KPRFGGPDYE EGPNSLINEE EFFDAVEAAL DRQDKIEEQS QSEKVRLHWP
360 370 380 390 400
TSLPSGDTFS SVGTHRFVQK PYSRSSSMSS IDLVSASDDV HRFSSQVEEM
410 420 430 440 450
VQNHMNYSLQ DVGGDANWQL VVEEGEMKVY RREVEENGIV LDPLKATHAV
460 470 480 490 500
KGVTGHEVCN YFWNVDVRND WETTIENFHV VETLADNAII VYQTHKRVWP
510 520 530 540 550
ASQRDVLYLS AIRKIPALTE NDPETWIVCN FSVDHDSAPL NNRCVRAKIN
560 570 580 590 600
IAMICQTLVS PPEGDQEISR DNILCKITYV ANVNPGGWAP ASVLRAVAKR
610 620
EYPKFLKRFT SYVQEKTAGK PILF
Length:624
Mass (Da):71,111
Last modified:March 1, 2001 - v1
Checksum:iFD708AFFB25FCD31
GO
Isoform 2 (identifier: Q9EQG9-2) [UniParc]FASTAAdd to basket
Also known as: Delta26, GPBPD26, CERTBy similarity

The sequence of this isoform differs from the canonical sequence as follows:
     371-396: Missing.

Show »
Length:598
Mass (Da):68,283
Checksum:i8474A05E5F5ADA73
GO
Isoform 3 (identifier: Q9EQG9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     311-313: EGP → VWT
     314-624: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:313
Mass (Da):35,929
Checksum:iA51478AAAE76358D
GO

Sequence cautioni

The sequence AAH16197 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti526W → S in BAB31070 (PubMed:16141072).Curated1
Sequence conflicti592S → P in BAB31070 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028735311 – 313EGP → VWT in isoform 3. 1 Publication3
Alternative sequenceiVSP_028736314 – 624Missing in isoform 3. 1 PublicationAdd BLAST311
Alternative sequenceiVSP_006277371 – 396Missing in isoform 2. 2 PublicationsAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232932 mRNA. Translation: AAG42048.1.
AF232934 mRNA. Translation: AAG42050.1.
AK012989 mRNA. Translation: BAB28581.1.
AK018103 mRNA. Translation: BAB31070.1.
AK038061 mRNA. Translation: BAC29927.1.
AK047100 mRNA. Translation: BAC32962.1.
AK080958 mRNA. Translation: BAC38095.2.
AK163624 mRNA. Translation: BAE37427.1.
BC016197 mRNA. Translation: AAH16197.1. Sequence problems.
CCDSiCCDS26705.1. [Q9EQG9-1]
CCDS49335.1. [Q9EQG9-2]
RefSeqiNP_001157694.1. NM_001164222.1. [Q9EQG9-2]
NP_075909.1. NM_023420.2. [Q9EQG9-1]
UniGeneiMm.24125.

Genome annotation databases

EnsembliENSMUST00000077672; ENSMUSP00000076856; ENSMUSG00000021669. [Q9EQG9-1]
ENSMUST00000109444; ENSMUSP00000105070; ENSMUSG00000021669. [Q9EQG9-2]
ENSMUST00000179226; ENSMUSP00000136766; ENSMUSG00000021669. [Q9EQG9-2]
GeneIDi68018.
KEGGimmu:68018.
UCSCiuc007rni.2. mouse. [Q9EQG9-3]
uc007rnk.2. mouse. [Q9EQG9-1]
uc007rnl.2. mouse. [Q9EQG9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiC43BP_MOUSE
AccessioniPrimary (citable) accession number: Q9EQG9
Secondary accession number(s): Q3TQF6
, Q8BNN8, Q8C8H3, Q8CAR3, Q91WB1, Q9CU52, Q9EQG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: March 1, 2001
Last modified: September 27, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot