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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 5

Gene

Enpp5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Zinc 1; catalyticBy similarity
Active sitei72 – 721NucleophileSequence analysis
Metal bindingi72 – 721Zinc 1; catalyticBy similarity
Metal bindingi191 – 1911Zinc 2; catalyticBy similarity
Metal bindingi195 – 1951Zinc 2; via tele nitrogen; catalyticBy similarity
Metal bindingi238 – 2381Zinc 1; catalyticBy similarity
Metal bindingi239 – 2391Zinc 1; via tele nitrogen; catalyticBy similarity
Metal bindingi339 – 3391Zinc 2; via tele nitrogen; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.6.1.9. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 5 (EC:3.1.-.-)
Short name:
E-NPP 5
Short name:
NPP-5
Gene namesi
Name:Enpp5Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1933830. Enpp5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei432 – 45221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424By similarityAdd
BLAST
Chaini25 – 477453Ectonucleotide pyrophosphatase/phosphodiesterase family member 5PRO_0000036402Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence analysis
Glycosylationi292 – 2921N-linked (GlcNAc...)Sequence analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence analysis
Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence analysis
Glycosylationi382 – 3821N-linked (GlcNAc...)Sequence analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9EQG7.
PaxDbiQ9EQG7.
PRIDEiQ9EQG7.

Expressioni

Tissue specificityi

Expressed abundantly in the brain and kidney, and at lower levels in the liver.1 Publication

Gene expression databases

BgeeiQ9EQG7.
ExpressionAtlasiQ9EQG7. baseline and differential.
GenevisibleiQ9EQG7. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9EQG7. 2 interactions.
MINTiMINT-4998189.
STRINGi10090.ENSMUSP00000024756.

Structurei

3D structure databases

ProteinModelPortaliQ9EQG7.
SMRiQ9EQG7. Positions 29-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOVERGENiHBG051485.
InParanoidiQ9EQG7.
KOiK18398.
OMAiKHLIHSQ.
OrthoDBiEOG7W153S.
TreeFamiTF330032.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029896. ENPP5.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF80. PTHR10151:SF80. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EQG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPEFLLASC TLATLCHSAP FSLQPEEQKV LVVSFDGFRW DYLYKVPTPH
60 70 80 90 100
FHYIMKNGVH VNQVTNVFIT KTYPNHYTLV TGLFAENHGI VANDMFDPIL
110 120 130 140 150
NKSFSLEHMD IYDSKFWEEA TPIWITNQRA GHASGAAMWP GADVKIHDSF
160 170 180 190 200
PTYYLPYNES VSFEDRVAKI IEWFTAKDPI NLGFLYWEEP DDTGHDVGPD
210 220 230 240 250
SPLMGSVISD VDHKLGYLIK MLKRAKLWNN VNLIVTSDHG MTQCSKQRVI
260 270 280 290 300
ELDRYLDKEH YTLIDHSPVA AILPKEGKFD EVYDALAGAH PNLTVYKKEE
310 320 330 340 350
IPERWHYKHN DRVQPIVAVA DEGWYILQNK SDDFLLGNHG YDNALAEMHP
360 370 380 390 400
IFLAHGPAFR KNFTKEAMNS TDLYSLLCHL LNLTALPHNG SFWNVQDLLS
410 420 430 440 450
SATPKPIPYT QSTTLLLGSD KPGEDEQEES YPYYIGVSLG SIIAMVFFVV
460 470
LIKHLIRSQV HTLQYRQVEV AQPLLQA
Length:477
Mass (Da):54,387
Last modified:November 24, 2009 - v3
Checksum:i98845AF8B725FD61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233377 mRNA. Translation: AAG49143.1.
CT030230 Genomic DNA. Translation: CAP19172.1.
BC138977 mRNA. Translation: AAI38978.1.
BC138978 mRNA. Translation: AAI38979.1.
CCDSiCCDS28802.1.
PIRiA59390.
RefSeqiNP_001162091.1. NM_001168620.1.
NP_114392.1. NM_032003.2.
UniGeneiMm.30145.

Genome annotation databases

EnsembliENSMUST00000024756; ENSMUSP00000024756; ENSMUSG00000023960.
ENSMUST00000154166; ENSMUSP00000122767; ENSMUSG00000023960.
GeneIDi83965.
KEGGimmu:83965.
UCSCiuc008cpr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233377 mRNA. Translation: AAG49143.1.
CT030230 Genomic DNA. Translation: CAP19172.1.
BC138977 mRNA. Translation: AAI38978.1.
BC138978 mRNA. Translation: AAI38979.1.
CCDSiCCDS28802.1.
PIRiA59390.
RefSeqiNP_001162091.1. NM_001168620.1.
NP_114392.1. NM_032003.2.
UniGeneiMm.30145.

3D structure databases

ProteinModelPortaliQ9EQG7.
SMRiQ9EQG7. Positions 29-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EQG7. 2 interactions.
MINTiMINT-4998189.
STRINGi10090.ENSMUSP00000024756.

Proteomic databases

MaxQBiQ9EQG7.
PaxDbiQ9EQG7.
PRIDEiQ9EQG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024756; ENSMUSP00000024756; ENSMUSG00000023960.
ENSMUST00000154166; ENSMUSP00000122767; ENSMUSG00000023960.
GeneIDi83965.
KEGGimmu:83965.
UCSCiuc008cpr.2. mouse.

Organism-specific databases

CTDi59084.
MGIiMGI:1933830. Enpp5.

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOVERGENiHBG051485.
InParanoidiQ9EQG7.
KOiK18398.
OMAiKHLIHSQ.
OrthoDBiEOG7W153S.
TreeFamiTF330032.

Enzyme and pathway databases

BRENDAi3.6.1.9. 3474.

Miscellaneous databases

ChiTaRSiEnpp5. mouse.
NextBioi350842.
PROiQ9EQG7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EQG7.
ExpressionAtlasiQ9EQG7. baseline and differential.
GenevisibleiQ9EQG7. MM.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029896. ENPP5.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF80. PTHR10151:SF80. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and catalytic similarities between nucleotide pyrophosphatases/phosphodiesterases and alkaline phosphatases."
    Gijsbers R., Ceulemans H., Stalmans W., Bollen M.
    J. Biol. Chem. 276:1361-1368(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J1 Publication.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Characterization of nucleotide pyrophosphatase-5 as an oligomannosidic glycoprotein in rat brain."
    Ohe Y., Ohnishi H., Okazawa H., Tomizawa K., Kobayashi H., Okawa K., Matozaki T.
    Biochem. Biophys. Res. Commun. 308:719-725(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney and Lung.

Entry informationi

Entry nameiENPP5_MOUSE
AccessioniPrimary (citable) accession number: Q9EQG7
Secondary accession number(s): A9C479, Q921P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 24, 2009
Last modified: November 11, 2015
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.