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Q9EQF5

- DPYS_MOUSE

UniProt

Q9EQF5 - DPYS_MOUSE

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Protein
Dihydropyrimidinase
Gene
Dpys
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate By similarity.

Catalytic activityi

5,6-dihydrouracil + H2O = 3-ureidopropanoate.

Cofactori

Binds 2 zinc ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Zinc 1 By similarity
Metal bindingi69 – 691Zinc 1 By similarity
Metal bindingi159 – 1591Zinc 1; via carbamate group By similarity
Metal bindingi159 – 1591Zinc 2; via carbamate group By similarity
Binding sitei164 – 1641Substrate By similarity
Metal bindingi192 – 1921Zinc 2 By similarity
Metal bindingi248 – 2481Zinc 2 By similarity
Metal bindingi326 – 3261Zinc 1 By similarity
Binding sitei347 – 3471Substrate; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. amino acid binding Source: BHF-UCL
  2. dihydropyrimidinase activity Source: BHF-UCL
  3. phosphoprotein binding Source: BHF-UCL
  4. thymine binding Source: BHF-UCL
  5. uracil binding Source: BHF-UCL
  6. zinc ion binding Source: BHF-UCL

GO - Biological processi

  1. beta-alanine metabolic process Source: BHF-UCL
  2. protein homooligomerization Source: BHF-UCL
  3. protein homotetramerization Source: BHF-UCL
  4. thymine catabolic process Source: BHF-UCL
  5. uracil catabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.2.2. 3474.

Protein family/group databases

MEROPSiM38.973.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropyrimidinase (EC:3.5.2.2)
Short name:
DHP
Short name:
DHPase
Alternative name(s):
Dihydropyrimidine amidohydrolase
Hydantoinase
Gene namesi
Name:Dpys
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1928679. Dpys.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: BHF-UCL
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Dihydropyrimidinase
PRO_0000165907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591N6-carboxylysine By similarity
Modified residuei256 – 2561N6-succinyllysine1 Publication

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two zinc ions By similarity.

Proteomic databases

MaxQBiQ9EQF5.
PaxDbiQ9EQF5.
PRIDEiQ9EQF5.

PTM databases

PhosphoSiteiQ9EQF5.

Expressioni

Gene expression databases

ArrayExpressiQ9EQF5.
BgeeiQ9EQF5.
CleanExiMM_DPYS.
GenevestigatoriQ9EQF5.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

BioGridi211101. 1 interaction.
IntActiQ9EQF5. 1 interaction.
MINTiMINT-1869933.

Structurei

3D structure databases

ProteinModelPortaliQ9EQF5.
SMRiQ9EQF5. Positions 6-493.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0044.
GeneTreeiENSGT00740000115468.
HOGENOMiHOG000219145.
HOVERGENiHBG000806.
InParanoidiQ9EQF5.
KOiK01464.
OMAiAVDYNIF.
OrthoDBiEOG7SJD48.
PhylomeDBiQ9EQF5.
TreeFamiTF314706.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
InterProiIPR006680. Amidohydro_1.
IPR011778. Hydantoinase/dihydroPyrase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
TIGRFAMsiTIGR02033. D-hydantoinase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9EQF5-1 [UniParc]FASTAAdd to Basket

« Hide

MAPQGRLLIR GGRIVNDDFS QVADVLVEDG VVRALGRDLL PPEDASRGLR    50
ILDAAGKLVL PGGIDTHTHM QFPFMGSQSV DDFYQGTKAA LAGGTTMIID 100
FAIPQKGSSL IEAFETWRNW ADPKVCCDYS LHVAVTWWSD KVKEEMKTLA 150
RDKGVNSFKM FMAYKGLYMV QDEQLYAAFS QCKEIGAIAQ VHAENGDLIA 200
EGAKKMLALG ITGPEGHELC RPEAVEAEAT LRAITIASAV NCPLYVVHVM 250
SKSAAKVVAD ARRAGNVVYG EPIAAGLGTD GRQYWSEEWS HAAHHVMGPP 300
LRPDPLTPGF LMDLLANGDL TTTGSDNCTF NTCQKALGKD DFTKIPNGVN 350
GVEDRMSVIW EKGVHSGKMD ENRFVAVTST NAAKIFNLYP KKGRIAVGSD 400
ADIVIWDPEA TRRISAKTHH QAVNFNIFEG MVCHGVPLVT ISRGRVVYEA 450
GVFNVTAGHG KFIPRQPFAE YIYKRIKQRD QTCTPVPVKR APYKGEVTTL 500
KARETKEDDT AGTRMQGHS 519
Length:519
Mass (Da):56,725
Last modified:June 6, 2002 - v2
Checksum:iF2C53D40EC120ABD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → L in AAG37999. 1 Publication
Sequence conflicti13 – 131R → L in AAH37086. 1 Publication
Sequence conflicti37 – 371R → P in AAK00644. 1 Publication
Sequence conflicti43 – 431E → G in AAK00644. 1 Publication
Sequence conflicti206 – 2061M → T in BAB23654. 1 Publication
Sequence conflicti299 – 2991P → L in AAK00644. 1 Publication
Sequence conflicti366 – 3661S → N in AAK00644. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF249296 mRNA. Translation: AAG37999.1.
AF227731 mRNA. Translation: AAK00644.1.
AK004822 mRNA. Translation: BAB23593.1.
AK004899 mRNA. Translation: BAB23654.1.
BC037086 mRNA. Translation: AAH37086.1.
CCDSiCCDS27445.1.
RefSeqiNP_001157938.1. NM_001164466.1.
NP_073559.3. NM_022722.3.
UniGeneiMm.275974.

Genome annotation databases

EnsembliENSMUST00000022915; ENSMUSP00000022915; ENSMUSG00000022304.
ENSMUST00000110306; ENSMUSP00000105935; ENSMUSG00000022304.
GeneIDi64705.
KEGGimmu:64705.
UCSCiuc007vok.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF249296 mRNA. Translation: AAG37999.1 .
AF227731 mRNA. Translation: AAK00644.1 .
AK004822 mRNA. Translation: BAB23593.1 .
AK004899 mRNA. Translation: BAB23654.1 .
BC037086 mRNA. Translation: AAH37086.1 .
CCDSi CCDS27445.1.
RefSeqi NP_001157938.1. NM_001164466.1.
NP_073559.3. NM_022722.3.
UniGenei Mm.275974.

3D structure databases

ProteinModelPortali Q9EQF5.
SMRi Q9EQF5. Positions 6-493.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211101. 1 interaction.
IntActi Q9EQF5. 1 interaction.
MINTi MINT-1869933.

Protein family/group databases

MEROPSi M38.973.

PTM databases

PhosphoSitei Q9EQF5.

Proteomic databases

MaxQBi Q9EQF5.
PaxDbi Q9EQF5.
PRIDEi Q9EQF5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022915 ; ENSMUSP00000022915 ; ENSMUSG00000022304 .
ENSMUST00000110306 ; ENSMUSP00000105935 ; ENSMUSG00000022304 .
GeneIDi 64705.
KEGGi mmu:64705.
UCSCi uc007vok.2. mouse.

Organism-specific databases

CTDi 1807.
MGIi MGI:1928679. Dpys.

Phylogenomic databases

eggNOGi COG0044.
GeneTreei ENSGT00740000115468.
HOGENOMi HOG000219145.
HOVERGENi HBG000806.
InParanoidi Q9EQF5.
KOi K01464.
OMAi AVDYNIF.
OrthoDBi EOG7SJD48.
PhylomeDBi Q9EQF5.
TreeFami TF314706.

Enzyme and pathway databases

BRENDAi 3.5.2.2. 3474.

Miscellaneous databases

NextBioi 320175.
PROi Q9EQF5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9EQF5.
Bgeei Q9EQF5.
CleanExi MM_DPYS.
Genevestigatori Q9EQF5.

Family and domain databases

Gene3Di 2.30.40.10. 2 hits.
InterProi IPR006680. Amidohydro_1.
IPR011778. Hydantoinase/dihydroPyrase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view ]
Pfami PF01979. Amidohydro_1. 1 hit.
[Graphical view ]
SUPFAMi SSF51338. SSF51338. 2 hits.
TIGRFAMsi TIGR02033. D-hydantoinase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of CRMP5, a novel member of the collapsin response mediator protein family."
    Fukada M., Watakabe I., Yuasa-Kawada J., Kawachi H., Kuroiwa A., Matsuda Y., Noda M.
    J. Biol. Chem. 275:37957-37965(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster / NIH.
    Tissue: Embryo.
  2. "Molecular cloning, nucleotide sequencing, characterization and overexpression of dihydropyrimidinase from mouse liver."
    Chang C.J., Huang C.J., Yang Y.S., Wu T.K.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver and Lung.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-256, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDPYS_MOUSE
AccessioniPrimary (citable) accession number: Q9EQF5
Secondary accession number(s): Q99PP1, Q9DBK3, Q9DBP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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