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Reviewed, UniProtKB/Swiss-Prot Q9EQE1 (SENP2_RAT)

Last modified January 19, 2010. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Sentrin-specific protease 2
    EC=3.4.22.-
Alternative name(s):
    Sentrin/SUMO-specific protease SENP2
    Axin-associating molecule
      Short name=Axam
Gene names
Name: Senp2
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Ref.2

Subunit structure

Binds to SUMO2 and SUMO3. Interacts with the C-terminal domain of NUP153 via its N-terminus By similarity. Binds to axin.

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side By similarity. Cytoplasm. Cytoplasmic vesicle. Note: Cytoplasmic and in cytoplasmic vesicles, together with axin. May shuttle between the nucleus and the cytoplasm.

Tissue specificity

Ubiquitous. Highly expressed in brain, lung and testis.

Post-translational modification

Polyubiquitinated; which leads to proteasomal degradation By similarity.

Sequence similarities

Belongs to the peptidase C48 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 588588Sentrin-specific protease 2
PRO_0000101720

Regions

Region72 – 381310Axin-binding
Region394 – 558165Protease
Motif28 – 314Nuclear localization signal Potential
Motif47 – 526Nuclear localization signal Potential
Motif316 – 33116Nuclear export signal By similarity

Sites

Active site4771 By similarity
Active site4941 By similarity
Active site5471Nucleophile By similarity

Amino acid modifications

Modified residue2171Phosphotyrosine By similarity
Modified residue3321Phosphoserine By similarity

Experimental info

Mutagenesis5471C → S: Abolishes protease activity. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9EQE1-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: BEC186367D8284C4

FASTA58867,252
        10         20         30         40         50         60 
MYRWLTKVLG TILRLCERPA PGARALLKRR RSSSSLFSTA VDTDEIPAKR PRLDCFIHQV 

        70         80         90        100        110        120 
KNSLYNAASL FGFPFQLTTK PMVSSACNGT RNVAPSGEVF SNSPSCELTT SGSCSSMLKL 

       130        140        150        160        170        180 
GNKSPNGISD YPKIRVTVAR DQPRRVLPSF GFTLKSEGYN RRPSGRRHSK SNPESSLPWK 

       190        200        210        220        230        240 
PQEQGVTEMI SEEGGKGARR PHCTVEEGVQ KDEREKYLKL LERLKEGAHG STFPPAVSHH 

       250        260        270        280        290        300 
SSQRTQMDTL KTKGWMEEQN HGVRTTHLVP KQYRVVETRG PLCSVRSEKR YSKGKADTEK 

       310        320        330        340        350        360 
VVGLRFEKDG TRGHQLEPDL SEEVSARLRL GSGSNGLLRR KISVLEAKEK NFPSKEKDRR 

       370        380        390        400        410        420 
TEDLFELTED MEKEISNALG HGPPDEILSS AFKLRITRGD IQTLKNYHWL NDEVINFYMN 

       430        440        450        460        470        480 
LLVERSKKQG YPALHALSTF FYPKLKSGGY QAVKRWTKGV NLFDQELVLV PIHRKVHWSL 

       490        500        510        520        530        540 
VVMDLRKKCL KYLDSMGQKG HRICEILLQY LQDESKTKRN TDLNLLEWTH YSMKPHEIPQ 

       550        560        570        580 
QLNGSDCGMF TCKYADYISR DKPITFTQHQ MPLFRKKMVW EILHQQLL 

« Hide

References

[1]"Inhibition of Wnt signaling pathway by a novel Axin-binding protein."
Kadoya T., Kishida S., Fukui A., Hinoi T., Michiue T., Asashima M., Kikuchi A.
J. Biol. Chem. 275:37030-37037(2000) [PubMed: 10944533] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Desumoylation activity of Axam, a novel Axin-binding protein, is involved in downregulation of beta-catenin."
Kadoya T., Yamamoto H., Suzuki T., Yukita A., Fukui A., Michiue T., Asahara T., Tanaka K., Asashima M., Kikuchi A.
Mol. Cell. Biol. 22:3803-3819(2002) [PubMed: 11997515] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF CYS-547.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF260129 mRNA. Translation: AAG34653.1.
IPIIPI00189898.
RefSeqNP_076479.1.
UniGeneRn.9367

3D structure databases

SMRQ9EQE1. Positions 363-588.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9EQE1.

Protein family/group databases

MEROPSC48.007.

PTM databases

PhosphoSiteQ9EQE1.

Genome annotation databases

EnsemblENSRNOT00000002425; ENSRNOP00000002425; ENSRNOG00000001773; Rattus norvegicus. [Genome view]
GeneID78973.
KEGGrno:78973.
UCSCNM_023989. rat.

Organism-specific databases

CTD78973.
RGD708378. Senp2.

Phylogenomic databases

eggNOGroNOG06700.
HOVERGENQ9EQE1.
InParanoidQ9EQE1.
OMAIDLRKKC.
OrthoDBEOG9SR0GC.

Gene expression databases

ArrayExpressQ9EQE1.
GenevestigatorQ9EQE1.
GermOnlineENSRNOG00000001773. Rattus norvegicus.

Family and domain databases

InterProIPR003653. Peptidase_C48.
[Graphical view]
PfamPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio614440.

Entry information

Entry nameSENP2_RAT
AccessionPrimary (citable) accession number: Q9EQE1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2001
Last modified: January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents