Reviewed, UniProtKB/Swiss-Prot Q9EQE1 (SENP2_RAT)

Names and origin

Protein names

Recommended name:
    Sentrin-specific protease 2
    EC=3.4.22.-
Alternative name(s):
    Sentrin/SUMO-specific protease SENP2
    Axin-associating molecule
      Short name=Axam

Gene names

Name:

Senp2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Ref.2
Subunit structure	Binds to SUMO2 and SUMO3. Interacts with the C-terminal domain of NUP153 via its N-terminus By similarity. Binds to axin.
Subcellular location	Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side By similarity. Cytoplasm. Cytoplasmic vesicle. Note: Cytoplasmic and in cytoplasmic vesicles, together with axin. May shuttle between the nucleus and the cytoplasm.
Tissue specificity	Ubiquitous. Highly expressed in brain, lung and testis.
Post-translational modification	Polyubiquitinated; which leads to proteasomal degradation By similarity.
Sequence similarities	Belongs to the peptidase C48 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport Ubl conjugation pathway Wnt signaling pathway mRNA transport
Cellular component	Cytoplasm Cytoplasmic vesicle Membrane Nuclear pore complex Nucleus
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear pore Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	SUMO-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB protein binding Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Sentrin-specific protease 2		PRO_0000101720
Regions
	Region	72 – 381	310	Axin-binding
	Region	394 – 558	165	Protease
	Motif	28 – 31	4	Nuclear localization signal Potential
	Motif	47 – 52	6	Nuclear localization signal Potential
	Motif	316 – 331	16	Nuclear export signal By similarity
Sites
	Active site	477	1	By similarity
	Active site	494	1	By similarity
	Active site	547	1	Nucleophile By similarity
Amino acid modifications
	Modified residue	217	1	Phosphotyrosine By similarity
	Modified residue	332	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	547	1	C → S: Abolishes protease activity. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9EQE1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BEC186367D8284C4	FASTA	588	67,252
	10 20 30 40 50 60 MYRWLTKVLG TILRLCERPA PGARALLKRR RSSSSLFSTA VDTDEIPAKR PRLDCFIHQV 70 80 90 100 110 120 KNSLYNAASL FGFPFQLTTK PMVSSACNGT RNVAPSGEVF SNSPSCELTT SGSCSSMLKL 130 140 150 160 170 180 GNKSPNGISD YPKIRVTVAR DQPRRVLPSF GFTLKSEGYN RRPSGRRHSK SNPESSLPWK 190 200 210 220 230 240 PQEQGVTEMI SEEGGKGARR PHCTVEEGVQ KDEREKYLKL LERLKEGAHG STFPPAVSHH 250 260 270 280 290 300 SSQRTQMDTL KTKGWMEEQN HGVRTTHLVP KQYRVVETRG PLCSVRSEKR YSKGKADTEK 310 320 330 340 350 360 VVGLRFEKDG TRGHQLEPDL SEEVSARLRL GSGSNGLLRR KISVLEAKEK NFPSKEKDRR 370 380 390 400 410 420 TEDLFELTED MEKEISNALG HGPPDEILSS AFKLRITRGD IQTLKNYHWL NDEVINFYMN 430 440 450 460 470 480 LLVERSKKQG YPALHALSTF FYPKLKSGGY QAVKRWTKGV NLFDQELVLV PIHRKVHWSL 490 500 510 520 530 540 VVMDLRKKCL KYLDSMGQKG HRICEILLQY LQDESKTKRN TDLNLLEWTH YSMKPHEIPQ 550 560 570 580 QLNGSDCGMF TCKYADYISR DKPITFTQHQ MPLFRKKMVW EILHQQLL

References

[1]	"Inhibition of Wnt signaling pathway by a novel Axin-binding protein." Kadoya T., Kishida S., Fukui A., Hinoi T., Michiue T., Asashima M., Kikuchi A. J. Biol. Chem. 275:37030-37037(2000) [PubMed: 10944533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Desumoylation activity of Axam, a novel Axin-binding protein, is involved in downregulation of beta-catenin." Kadoya T., Yamamoto H., Suzuki T., Yukita A., Fukui A., Michiue T., Asahara T., Tanaka K., Asashima M., Kikuchi A. Mol. Cell. Biol. 22:3803-3819(2002) [PubMed: 11997515] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-547.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF260129 mRNA. Translation: AAG34653.1.
IPI	IPI00189898.
RefSeq	NP_076479.1.
UniGene	Rn.9367
3D structure databases
SMR	Q9EQE1. Positions 363-588.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9EQE1.
Protein family/group databases
MEROPS	C48.007.
PTM databases
PhosphoSite	Q9EQE1.
Genome annotation databases
Ensembl	ENSRNOT00000002425; ENSRNOP00000002425; ENSRNOG00000001773; Rattus norvegicus. [Genome view]
GeneID	78973.
KEGG	rno:78973.
UCSC	NM_023989. rat.
Organism-specific databases
CTD	78973.
RGD	708378. Senp2.
Phylogenomic databases
eggNOG	roNOG06700.
HOVERGEN	Q9EQE1.
InParanoid	Q9EQE1.
OMA	IDLRKKC.
OrthoDB	EOG9SR0GC.
Gene expression databases
ArrayExpress	Q9EQE1.
Genevestigator	Q9EQE1.
GermOnline	ENSRNOG00000001773. Rattus norvegicus.
Family and domain databases
InterPro	IPR003653. Peptidase_C48. [Graphical view]
Pfam	PF02902. Peptidase_C48. 1 hit. [Graphical view]
PROSITE	PS50600. ULP_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	614440.

Entry information

Entry name

SENP2_RAT

Accession

Primary (citable) accession number: Q9EQE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)