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Protein

Neuropeptide FF receptor 2

Gene

Npffr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-389397. Orexin and neuropeptides FF and QRFP bind to their respective receptors.
R-RNO-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptide FF receptor 2
Alternative name(s):
G-protein coupled receptor 74
Neuropeptide G-protein coupled receptor
Gene namesi
Name:Npffr2
Synonyms:Gpr74, Npff2, Npgpr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi620168. Npffr2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45ExtracellularSequence analysisAdd BLAST45
Transmembranei46 – 66Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini67 – 82CytoplasmicSequence analysisAdd BLAST16
Transmembranei83 – 103Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini104 – 119ExtracellularSequence analysisAdd BLAST16
Transmembranei120 – 140Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini141 – 160CytoplasmicSequence analysisAdd BLAST20
Transmembranei161 – 181Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini182 – 217ExtracellularSequence analysisAdd BLAST36
Transmembranei218 – 238Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini239 – 274CytoplasmicSequence analysisAdd BLAST36
Transmembranei275 – 295Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini296 – 310ExtracellularSequence analysisAdd BLAST15
Transmembranei311 – 331Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini332 – 417CytoplasmicSequence analysisAdd BLAST86

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3425.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699171 – 417Neuropeptide FF receptor 2Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi20N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi31N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi118 ↔ 206PROSITE-ProRule annotation
Glycosylationi198N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9EQD2.
PRIDEiQ9EQD2.

PTM databases

PhosphoSitePlusiQ9EQD2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003067.
GenevisibleiQ9EQD2. RN.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004153.

Chemistry databases

BindingDBiQ9EQD2.

Structurei

3D structure databases

ProteinModelPortaliQ9EQD2.
SMRiQ9EQD2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00900000140797.
HOGENOMiHOG000041285.
HOVERGENiHBG099624.
InParanoidiQ9EQD2.
KOiK08375.
OMAiHWLAFGN.
OrthoDBiEOG091G098F.
PhylomeDBiQ9EQD2.
TreeFamiTF315303.

Family and domain databases

InterProiView protein in InterPro
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005395. NPFF_rcpt.
IPR005397. NPFF_rcpt_2.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00237. GPCRRHODOPSN.
PR01570. NPFFRECEPTOR.
PR01572. NPFFRECEPTR2.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9EQD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRWDSNSS GSWDHIWSGN DTQHPWYSDI NITYMNYYLH QPHVTAVFIS
60 70 80 90 100
SYFLIFFLCM VGNTVVCFVV IRNRYMHTVT NFFIFNLAIS DLLVGIFCMP
110 120 130 140 150
ITLLDNIIAG WPFGSSMCKI SGLVQGISVA ASVFTLVAIA VDRFRCVVYP
160 170 180 190 200
FKPKLTVKTA FVMIVIIWGL AITIMTPSAI MLHVQEEKYY RVRLSSHNKT
210 220 230 240 250
STVYWCREDW PNQEMRRIYT TVLFATIYLA PLSLIVIMYA RIGASLFKTS
260 270 280 290 300
AHSTGKQRLE QWHVSKKKQK VIKMLLTVAL LFILSWLPLW TLMMLSDYAD
310 320 330 340 350
LSPNKLRVIN IYVYPFAHWL AFCNSSVNPI IYGFFNENFR SGFQDAFQFC
360 370 380 390 400
QKKVKPQEAY GLRAKRNLDI NTSGLLVHEP ASQNPSGENL GCRKSADNPT
410
QESLMEETGE ATNSTET
Length:417
Mass (Da):47,711
Last modified:March 1, 2001 - v1
Checksum:iDFEB6AC63AF2AAD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF268900 mRNA. Translation: AAG41399.1.
RefSeqiNP_076470.1. NM_023980.1.
XP_006250849.1. XM_006250787.3.
XP_017454875.1. XM_017599386.1.
UniGeneiRn.64524.

Genome annotation databases

EnsembliENSRNOT00000004153; ENSRNOP00000004153; ENSRNOG00000003067.
GeneIDi78964.
KEGGirno:78964.
UCSCiRGD:620168. rat.

Similar proteinsi

Entry informationi

Entry nameiNPFF2_RAT
AccessioniPrimary (citable) accession number: Q9EQD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families