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Reviewed, UniProtKB/Swiss-Prot Q9EQD0 (FZD5_MOUSE)

Last modified February 9, 2010. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Frizzled-5
      Short name=Fz-5
      Short name=mFz5
Gene names
Name: Fzd5
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in yolk sac angiogenesis and in placental vascularization. Binds to Wnt2, Wnt10B, Wnt5A, but not to Wnt2B or Wnt4.

Subunit structure

Interacts with GOPC. Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note: Localized at the plasma membrane and also found at the Golgi. Ref.6

Tissue specificity

Expressed in eye, kidney, lung, chondrocytes, epithelial cells of the small intestine and gobelet cells of the colon. Ref.5

Developmental stage

Expressed in the yolk sac, placenta, eye and lung bud at 9.5 dpc. At 10.5 dpc, also expressed in the placental blood vessel of embryonic origin.

Domain

The PDZ-binding motif mediates interaction with GOPC By similarity.

The FZ domain is involved in binding with Wnt ligands By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Sequence caution

The sequence AAG39355.1 differs from that shown. Reason: Frameshift at several positions.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 585559Frizzled-5
PRO_0000243936

Regions

Topological domain27 – 238212Extracellular Potential
Transmembrane239 – 259211 Potential
Topological domain260 – 27011Cytoplasmic Potential
Transmembrane271 – 291212 Potential
Topological domain292 – 31524Extracellular Potential
Transmembrane316 – 336213 Potential
Topological domain337 – 35822Cytoplasmic Potential
Transmembrane359 – 379214 Potential
Topological domain380 – 40223Extracellular Potential
Transmembrane403 – 423215 Potential
Topological domain424 – 44926Cytoplasmic Potential
Transmembrane450 – 470216 Potential
Topological domain471 – 50030Extracellular Potential
Transmembrane501 – 521217 Potential
Topological domain522 – 58564Cytoplasmic Potential
Domain28 – 150123FZ
Motif582 – 5843PDZ-binding By similarity

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 94 By similarity
Disulfide bond41 ↔ 87 By similarity
Disulfide bond78 ↔ 116 By similarity
Disulfide bond105 ↔ 147 By similarity
Disulfide bond109 ↔ 133 By similarity

Experimental info

Sequence conflict41P → T in BAC26789. Ref.3
Sequence conflict123 – 1242YG → S in BAC53981. Ref.2
Sequence conflict3931V → G in BAC26789. Ref.3
Sequence conflict4191L → H in BAC53981. Ref.2
Sequence conflict4441E → G in BAC53981. Ref.2
Sequence conflict5211I → F in BAC53981. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q9EQD0-1 [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: B065362F05AD7899

FASTA58564,138
        10         20         30         40         50         60 
MARPDPSAPP SLLLLLLAQL VGRAAAASKA PVCQEITVPM CRGIGYNLTH MPNQFNHDTQ 

        70         80         90        100        110        120 
DEAGLEVHQF WPLVEIHCSP DLRFFLCSMY TPICLPDYHK PLPPCRSVCE RAKAGCSPLM 

       130        140        150        160        170        180 
RQYGFAWPER MSCDRLPVLG GDAEVLCMDY NRSEATTASP KSFPAKPTLP GPPGAPSSGG 

       190        200        210        220        230        240 
ECPSGGPSVC TCREPFVPIL KESHPLYNKV RTGQVPNCAV PCYQPSFSPD ERTFATFWIG 

       250        260        270        280        290        300 
LWSVLCFIST STTVATFLID MERFRYPERP IIFLSACYLC VSLGFLVRLV VGHASVACSR 

       310        320        330        340        350        360 
EHSHIHYETT GPALCTVVFL LVYFFGMASS IWWVILSLTW FLAAGMKWGN EAIAGYAQYF 

       370        380        390        400        410        420 
HLAAWLIPSV KSITALALSS VDGDPVAGIC YGVNQNLNSL RGFVLGPLVL YLLVGTLFLL 

       430        440        450        460        470        480 
AGFVSLFRIR SVIKQGGTKT DKLEKLMIRI GIFTLLYTVP ASIVVACYLY EQHYRESWEA 

       490        500        510        520        530        540 
ALTCACPGPD AGQPRAKPEY WVLMLKYFMC LVVGITSGVW IWSGKTLESW RRFTSRCCCS 

       550        560        570        580 
SRRGHKSGGA MAAGDYAEAS AALTGRTGPP GPTAAYHKQV SLSHV

« Hide

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References

« Hide 'large scale' references
[1]"Mouse Wnt receptor gene Fzd5 is essential for yolk sac and placental angiogenesis."
Ishikawa T., Tamai Y., Zorn A.M., Yoshida H., Seldin M.F., Nishikawa S., Taketo M.M.
Development 128:25-33(2001) [PubMed: 11092808] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6N.
Tissue: Intestine.
[2]"Molecular cloning and characterization of a gene encoding for rat Frizzled 5."
Ito S., Imamura T., Shiota K.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[4]Johnson M.A., Greenberg N.M.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 211-300.
Strain: C57BL/6.
Tissue: Prostate.
[5]"A large family of putative transmembrane receptors homologous to the product of the Drosophila tissue polarity gene frizzled."
Wang Y., Macke J.P., Abella B.S., Andreasson K., Worley P., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J.
J. Biol. Chem. 271:4468-4476(1996) [PubMed: 8626800] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Identification of a PDZ domain containing Golgi protein, GOPC, as an interaction partner of frizzled."
Yao R., Maeda T., Takada S., Noda T.
Biochem. Biophys. Res. Commun. 286:771-778(2001) [PubMed: 11520064] [Abstract]
Cited for: INTERACTION WITH GOPC, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF272146 mRNA. Translation: AAG39355.1. Frameshift.
AB052910 mRNA. Translation: BAC53981.1.
AK030111 mRNA. Translation: BAC26789.1.
AF005203 mRNA. Translation: AAC01953.1.
IPIIPI00318553.
RefSeqNP_001036124.1.
NP_073558.2.
UniGeneMm.150813
Mm.470210

3D structure databases

SMRQ9EQD0. Positions 33-152.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9EQD0.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9EQD0.

Proteomic databases

PRIDEQ9EQD0.

Genome annotation databases

EnsemblENSMUST00000063982; ENSMUSP00000067783; ENSMUSG00000045005; Mus musculus. [Genome view]
GeneID14367.
KEGGmmu:14367.

Organism-specific databases

CTD14367.
MGIMGI:108571. Fzd5.

Phylogenomic databases

eggNOGroNOG07080.
HOGENOMHBG447413.
HOVERGENQ9EQD0.
InParanoidQ9EQD0.

Gene expression databases

ArrayExpressQ9EQD0.
BgeeQ9EQD0.
CleanExMM_FZD5.
GenevestigatorQ9EQD0.

Family and domain databases

InterProIPR000539. Frizzled.
IPR020067. Frizzled-like_dom.
IPR000024. Frizzled_Cys-rich.
IPR020068. Frizzled_dom_subgr.
IPR015526. Frizzled_related.
IPR017981. GPCR_2-like.
[Graphical view]
Gene3DG3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHERPTHR11309. Fz_related. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio285839.
SOURCESearch...

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Entry information

Entry nameFZD5_MOUSE
AccessionPrimary (citable) accession number: Q9EQD0
Secondary accession number(s): O08975, Q8BMR2, Q8CHK9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: June 27, 2006
Last modified: February 9, 2010
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents