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Protein

N-terminal kinase-like protein

Gene

Scyl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal kinase-like protein
Alternative name(s):
105 kDa kinase-like protein
Mitosis-associated kinase-like protein NTKL
SCY1-like protein 1
Gene namesi
Name:Scyl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1931787. Scyl1.

Subcellular locationi

GO - Cellular componenti

  • cis-Golgi network Source: UniProtKB
  • COPI vesicle coat Source: UniProtKB
  • cytoplasm Source: HGNC
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • membrane Source: MGI
  • microtubule organizing center Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus

Pathology & Biotechi

Involvement in diseasei

Defects in Scyl1 are the cause of the muscle deficient phenotype (mdf). Mice exhibit progressive neuromuscular atrophy, hindlimb paralysis, gait ataxia, abnormal hindlimb posture and tremor. Pathology of mdf comprises cerebellar atrophy, Purkinje cell loss and optic nerve atrophy.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi791 – 7922KK → AA: Decreased interaction with COPB1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 806806N-terminal kinase-like proteinPRO_0000249542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei752 – 7521PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9EQC5.
MaxQBiQ9EQC5.
PaxDbiQ9EQC5.
PeptideAtlasiQ9EQC5.
PRIDEiQ9EQC5.

PTM databases

iPTMnetiQ9EQC5.
PhosphoSiteiQ9EQC5.

Expressioni

Tissue specificityi

Expressed in diaphragm, quadriceps, thymus, liver, lung, spleen, kidney, heart and brain. Prominently expressed in neurons, and enriched at central nervous system synapses and neuromuscular junctions.2 Publications

Gene expression databases

BgeeiQ9EQC5.
CleanExiMM_SCYL1.
GenevisibleiQ9EQC5. MM.

Interactioni

Subunit structurei

Homooligomer (By similarity). Interacts with GORAB. Interacts with COPA, COPB1 and COPB2. Interacts with AP2B1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi219686. 2 interactions.
STRINGi10090.ENSMUSP00000025890.

Structurei

3D structure databases

ProteinModelPortaliQ9EQC5.
SMRiQ9EQC5. Positions 34-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 314301Protein kinasePROSITE-ProRule annotationAdd
BLAST
Repeati350 – 38839HEAT 1Add
BLAST
Repeati389 – 42739HEAT 2Add
BLAST
Repeati507 – 54539HEAT 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni791 – 80616Interaction with COPB1Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili755 – 79541Sequence analysisAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 3 HEAT repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1243. Eukaryota.
ENOG410XQTG. LUCA.
GeneTreeiENSGT00760000119345.
HOGENOMiHOG000208340.
HOVERGENiHBG082065.
InParanoidiQ9EQC5.
KOiK08876.
OMAiNEVRDGW.
OrthoDBiEOG7RJPQX.
PhylomeDBiQ9EQC5.
TreeFamiTF313435.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFFARDPVR DFPFELSPEP PEGGPPGPWI LHRGRKKATG SAVSIFVYDV
60 70 80 90 100
KPGAEEQTQV AKAAFKRLKT LRHPNILAYI DGLETEKCLH IVTEAVTPLG
110 120 130 140 150
TYLKARAEAG GLKEQELSWG LHQIVKALSF LVNDCNLIHN NVCMAAVFVD
160 170 180 190 200
RAGEWKLGGL DYMYSAQGNG GGPPSKGIPE LEQYDPPELA DSSSRAVREK
210 220 230 240 250
WSADMWRLGC LIWEVFNGSL PRAAALRNPG KIPKSLVTHY CELVGANPKV
260 270 280 290 300
RPNPARFLQN CRAPGGFMSN RFVETNLFLE EIQIKEPAEK QKFFQELSKS
310 320 330 340 350
LDSFPEDFCR HKVLPQLLTA FEFGNAGAVV LTPLFKVGKS LRAEEYQEKI
360 370 380 390 400
IPVVVKMFSS TDRAMRIRLL QQMEQFIQYL DEPTVNTQIF PHVTHGFLDT
410 420 430 440 450
NPAIREQTVK SMLLLAPKLN EANLNVELMK HFARLQAKDD QGPIRCNTTV
460 470 480 490 500
CLGKIGSYLS ASTRHRVLTS AFSRATKDPF APSRVAGVLG FAATHNLYSM
510 520 530 540 550
DDCAHKILPV LCGLTVDPEK SVRDQAFKTI RSFLSKLESV SEDPTQLAEV
560 570 580 590 600
EKDVHAASSP GTGGAAASWA GWAVTGVSSL TSKLIRAHPT PVPSDTTVPQ
610 620 630 640 650
RPVPEGNPAP APALAQAIPA TSGHWETQED KDTAEDSATA DRWDDEDWGS
660 670 680 690 700
LEQEAESVLA QQDDWSAKGQ GSRAGQINHP DHKSLESHWS SWEVEGSWDQ
710 720 730 740 750
GWQEPSSVEP PPEGTRLASE YNWGGAEPSD KGDPFAALSV RPSAQPRPDP
760 770 780 790 800
DSWGEDNWEG LEAESRQVKA ELARKKREER RREMEAKRAE KKTTKGPMKL

GARKLD
Length:806
Mass (Da):89,160
Last modified:March 1, 2001 - v1
Checksum:i71B559E6066B4E4E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871P → Q in BAE26711 (PubMed:16141072).Curated
Sequence conflicti642 – 6421R → K in BAE22111 (PubMed:16141072).Curated
Sequence conflicti642 – 6421R → K in BAE36588 (PubMed:16141072).Curated
Sequence conflicti653 – 6531Missing in BAE22111 (PubMed:16141072).Curated
Sequence conflicti653 – 6531Missing in BAE36588 (PubMed:16141072).Curated
Sequence conflicti677 – 6771I → V in AAH34519 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276514 mRNA. Translation: AAG17393.1.
AK134356 mRNA. Translation: BAE22111.1.
AK144134 mRNA. Translation: BAE25720.1.
AK145869 mRNA. Translation: BAE26711.1.
AK161813 mRNA. Translation: BAE36588.1.
BC034519 mRNA. Translation: AAH34519.1.
CCDSiCCDS29480.1.
RefSeqiNP_076401.1. NM_023912.2.
UniGeneiMm.276063.

Genome annotation databases

EnsembliENSMUST00000025890; ENSMUSP00000025890; ENSMUSG00000024941.
GeneIDi78891.
KEGGimmu:78891.
UCSCiuc008gfh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276514 mRNA. Translation: AAG17393.1.
AK134356 mRNA. Translation: BAE22111.1.
AK144134 mRNA. Translation: BAE25720.1.
AK145869 mRNA. Translation: BAE26711.1.
AK161813 mRNA. Translation: BAE36588.1.
BC034519 mRNA. Translation: AAH34519.1.
CCDSiCCDS29480.1.
RefSeqiNP_076401.1. NM_023912.2.
UniGeneiMm.276063.

3D structure databases

ProteinModelPortaliQ9EQC5.
SMRiQ9EQC5. Positions 34-285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219686. 2 interactions.
STRINGi10090.ENSMUSP00000025890.

PTM databases

iPTMnetiQ9EQC5.
PhosphoSiteiQ9EQC5.

Proteomic databases

EPDiQ9EQC5.
MaxQBiQ9EQC5.
PaxDbiQ9EQC5.
PeptideAtlasiQ9EQC5.
PRIDEiQ9EQC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025890; ENSMUSP00000025890; ENSMUSG00000024941.
GeneIDi78891.
KEGGimmu:78891.
UCSCiuc008gfh.1. mouse.

Organism-specific databases

CTDi57410.
MGIiMGI:1931787. Scyl1.

Phylogenomic databases

eggNOGiKOG1243. Eukaryota.
ENOG410XQTG. LUCA.
GeneTreeiENSGT00760000119345.
HOGENOMiHOG000208340.
HOVERGENiHBG082065.
InParanoidiQ9EQC5.
KOiK08876.
OMAiNEVRDGW.
OrthoDBiEOG7RJPQX.
PhylomeDBiQ9EQC5.
TreeFamiTF313435.

Miscellaneous databases

PROiQ9EQC5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EQC5.
CleanExiMM_SCYL1.
GenevisibleiQ9EQC5. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and preliminary characterization of a 105 kDa protein with an N-terminal kinase-like domain."
    Liu S.C.H., Lane W.S., Lienhard G.E.
    Biochim. Biophys. Acta 1517:148-152(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Adipocyte.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Fetal testis, Leydig cell and Placenta.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Cloning and characterization of a novel gene which encodes a protein interacting with the mitosis-associated kinase-like protein NTKL."
    Di Y., Li J., Fang J., Xu Z., He X., Zhang F., Ling J., Li X., Xu D., Li L., Li Y.Y., Huo K.
    J. Hum. Genet. 48:315-321(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GORAB, SUBCELLULAR LOCATION.
  5. "Mutation in the Scyl1 gene encoding amino-terminal kinase-like protein causes a recessive form of spinocerebellar neurodegeneration."
    Schmidt W.M., Kraus C., Hoeger H., Hochmeister S., Oberndorfer F., Branka M., Bingemann S., Lassmann H., Mueller M., Macedo-Souza L.I., Vainzof M., Zatz M., Reis A., Bittner R.E.
    EMBO Rep. 8:691-697(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISEASE, TISSUE SPECIFICITY.
  6. "Scyl1, mutated in a recessive form of spinocerebellar neurodegeneration, regulates COPI-mediated retrograde traffic."
    Burman J.L., Bourbonniere L., Philie J., Stroh T., Dejgaard S.Y., Presley J.F., McPherson P.S.
    J. Biol. Chem. 283:22774-22786(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH COPA; COPB1 AND COPB2, MUTAGENESIS OF 791-LYS-LYS-792.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiNTKL_MOUSE
AccessioniPrimary (citable) accession number: Q9EQC5
Secondary accession number(s): Q3TST7, Q3UKU0, Q8K222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.