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Protein

3 beta-hydroxysteroid dehydrogenase type 7

Gene

Hsd3b7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis (PubMed:11067870). Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).2 Publications

Catalytic activityi

3-beta-hydroxy-Delta(5)-steroid + NAD+ = 3-oxo-Delta(5)-steroid + NADH.
Cholest-5-ene-3-beta,7-alpha-diol + NAD+ = 7-alpha-hydroxycholest-4-en-3-one + NADH.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei159Proton acceptorBy similarity1
Binding sitei163NADBy similarity1

GO - Molecular functioni

  • 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: InterPro
  • cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase activity Source: UniProtKB

GO - Biological processi

  • B cell chemotaxis Source: UniProtKB
  • steroid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
UniPathwayiUPA00062.

Chemistry databases

SwissLipidsiSLP:000001322.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase type 7
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase type VII
Short name:
3-beta-HSD VII
3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase (EC:1.1.1.-)
Short name:
C(27) 3-beta-HSD
Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase (EC:1.1.1.181)
Gene namesi
Name:Hsd3b7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2141879. Hsd3b7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei289 – 309HelicalSequence analysisAdd BLAST21
Transmembranei312 – 334HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877921 – 3693 beta-hydroxysteroid dehydrogenase type 7Add BLAST369

Proteomic databases

MaxQBiQ9EQC1.
PaxDbiQ9EQC1.
PRIDEiQ9EQC1.

PTM databases

iPTMnetiQ9EQC1.
PhosphoSitePlusiQ9EQC1.

Expressioni

Tissue specificityi

Predominantly expressed in liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000042289.
ExpressionAtlasiQ9EQC1. baseline and differential.
GenevisibleiQ9EQC1. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9EQC1. 542 interactors.
MINTiMINT-1845620.
STRINGi10090.ENSMUSP00000036245.

Structurei

3D structure databases

ProteinModelPortaliQ9EQC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiQ9EQC1.
KOiK12408.
OMAiGENHELM.
OrthoDBiEOG091G09QZ.
PhylomeDBiQ9EQC1.
TreeFamiTF354279.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9EQC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSAQVPTL VYLVTGGCGF LGEHIVRMLL EREPRLRELR VFDLHLSSWL
60 70 80 90 100
EELKAGPVQV TAIQGDVTQA HEVAAAMSGS HVVIHTAGLV DVFGKASPKT
110 120 130 140 150
IHKVNVQGTQ NVIDACVQTG TQYLVYTSSM EVVGPNIKGH PFYRGNEDTP
160 170 180 190 200
YEAVHSHPYP CSKALAEQLV LEANGRKVNG GLPLVTCALR PTGIYGEGHQ
210 220 230 240 250
VMRDFYYQGL RFGGRLFRAV PASVEHGRVY VGNVAWMHIL VARELEQRAA
260 270 280 290 300
LMGGQVYFCY DKSPYKSYED FNMEFLSPCG LRLIGAHPLL PYWLLVLLAT
310 320 330 340 350
LNALLQWLLR PLVLYTPLLN PYTLAMANTT FTVSTNKAQR HFGYKPLFSW
360
EESRTRTIQW VQAMEGSAR
Length:369
Mass (Da):41,135
Last modified:March 1, 2001 - v1
Checksum:i5857A690E0600F13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF277718 mRNA. Translation: AAG37823.1.
BC132605 mRNA. Translation: AAI32606.1.
BC138589 mRNA. Translation: AAI38590.1.
CCDSiCCDS40145.1.
RefSeqiNP_598704.2. NM_133943.2.
UniGeneiMm.157103.

Genome annotation databases

EnsembliENSMUST00000046863; ENSMUSP00000036245; ENSMUSG00000042289.
GeneIDi101502.
KEGGimmu:101502.
UCSCiuc009jwu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF277718 mRNA. Translation: AAG37823.1.
BC132605 mRNA. Translation: AAI32606.1.
BC138589 mRNA. Translation: AAI38590.1.
CCDSiCCDS40145.1.
RefSeqiNP_598704.2. NM_133943.2.
UniGeneiMm.157103.

3D structure databases

ProteinModelPortaliQ9EQC1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EQC1. 542 interactors.
MINTiMINT-1845620.
STRINGi10090.ENSMUSP00000036245.

Chemistry databases

SwissLipidsiSLP:000001322.

PTM databases

iPTMnetiQ9EQC1.
PhosphoSitePlusiQ9EQC1.

Proteomic databases

MaxQBiQ9EQC1.
PaxDbiQ9EQC1.
PRIDEiQ9EQC1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046863; ENSMUSP00000036245; ENSMUSG00000042289.
GeneIDi101502.
KEGGimmu:101502.
UCSCiuc009jwu.1. mouse.

Organism-specific databases

CTDi80270.
MGIiMGI:2141879. Hsd3b7.

Phylogenomic databases

eggNOGiKOG1430. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiQ9EQC1.
KOiK12408.
OMAiGENHELM.
OrthoDBiEOG091G09QZ.
PhylomeDBiQ9EQC1.
TreeFamiTF354279.

Enzyme and pathway databases

UniPathwayiUPA00062.
ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

Miscellaneous databases

ChiTaRSiHsd3b7. mouse.
PROiQ9EQC1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042289.
ExpressionAtlasiQ9EQC1. baseline and differential.
GenevisibleiQ9EQC1. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry namei3BHS7_MOUSE
AccessioniPrimary (citable) accession number: Q9EQC1
Secondary accession number(s): A2RTR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.