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Protein

Carbohydrate sulfotransferase 1

Gene

Chst1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 75PAPSBy similarity7
Nucleotide bindingi234 – 242PAPSBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 1 (EC:2.8.2.21)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
Short name:
GST-1
Keratan sulfate Gal-6 sulfotransferase
Short name:
KS6ST
Short name:
KSGal6ST
Short name:
KSST
Gene namesi
Name:Chst1
Synonyms:Gst1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924219. Chst1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 2CytoplasmicSequence analysis2
Transmembranei3 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini24 – 411LumenalSequence analysisAdd BLAST388

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851831 – 411Carbohydrate sulfotransferase 1Add BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...)Sequence analysis1
Glycosylationi145N-linked (GlcNAc...)Sequence analysis1
Glycosylationi189N-linked (GlcNAc...)Sequence analysis1
Glycosylationi334N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9EQC0.
PRIDEiQ9EQC0.

PTM databases

PhosphoSitePlusiQ9EQC0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027221.
ExpressionAtlasiQ9EQC0. baseline and differential.
GenevisibleiQ9EQC0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000064246.

Structurei

3D structure databases

ProteinModelPortaliQ9EQC0.
SMRiQ9EQC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi337 – 339Cell attachment siteSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHC0. Eukaryota.
ENOG4110V0B. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiQ9EQC0.
KOiK01022.
OMAiAKHKFGT.
OrthoDBiEOG091G0V3Y.
PhylomeDBiQ9EQC0.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9EQC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLTDT GLAERLCEEG
60 70 80 90 100
PTFSYNLSRK THVLILATTR SGSSFVGQLF NQHMDVFYLF EPLYHVQNTL
110 120 130 140 150
IPRFTQGKSP ADRRVMLGAS RDLLRSLYDC DLYFLENYIK PPPVNHTTNR
160 170 180 190 200
VFRRGASRVL CSRPVCDPPG SSDLILEEGD CVRMCGLLNL TLAAEACRER
210 220 230 240 250
SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL ASRSETFRDT
260 270 280 290 300
YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS SSVSTGLMRP SWLKGKYMLV
310 320 330 340 350
RYEDLARNPM KKTEEIYEFL GIPLDSHVAH WIQNNTRGDP TLGKHKYSTV
360 370 380 390 400
RNSAATAEKW RFRLSYDIVA FAQNACQQVL AQLGYKMANS EEELKNPAIS
410
LVEERDFRPF L
Length:411
Mass (Da):46,904
Last modified:March 1, 2001 - v1
Checksum:iB1AE590EF5B9CBDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF280087 mRNA. Translation: AAG48245.1.
BC030667 mRNA. Translation: AAH30667.1.
AK011344 mRNA. Translation: BAB27556.2.
CCDSiCCDS16450.1.
RefSeqiNP_076339.1. NM_023850.2.
XP_006500451.1. XM_006500388.1.
XP_006500452.1. XM_006500389.1.
UniGeneiMm.38021.

Genome annotation databases

EnsembliENSMUST00000065797; ENSMUSP00000064246; ENSMUSG00000027221.
GeneIDi76969.
KEGGimmu:76969.
UCSCiuc008lfk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF280087 mRNA. Translation: AAG48245.1.
BC030667 mRNA. Translation: AAH30667.1.
AK011344 mRNA. Translation: BAB27556.2.
CCDSiCCDS16450.1.
RefSeqiNP_076339.1. NM_023850.2.
XP_006500451.1. XM_006500388.1.
XP_006500452.1. XM_006500389.1.
UniGeneiMm.38021.

3D structure databases

ProteinModelPortaliQ9EQC0.
SMRiQ9EQC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000064246.

PTM databases

PhosphoSitePlusiQ9EQC0.

Proteomic databases

PaxDbiQ9EQC0.
PRIDEiQ9EQC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065797; ENSMUSP00000064246; ENSMUSG00000027221.
GeneIDi76969.
KEGGimmu:76969.
UCSCiuc008lfk.2. mouse.

Organism-specific databases

CTDi8534.
MGIiMGI:1924219. Chst1.

Phylogenomic databases

eggNOGiENOG410IHC0. Eukaryota.
ENOG4110V0B. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiQ9EQC0.
KOiK01022.
OMAiAKHKFGT.
OrthoDBiEOG091G0V3Y.
PhylomeDBiQ9EQC0.
TreeFamiTF342871.

Enzyme and pathway databases

ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.

Miscellaneous databases

PROiQ9EQC0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027221.
ExpressionAtlasiQ9EQC0. baseline and differential.
GenevisibleiQ9EQC0. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCHST1_MOUSE
AccessioniPrimary (citable) accession number: Q9EQC0
Secondary accession number(s): Q9D0K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.