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Protein

Dimethylaniline monooxygenase [N-oxide-forming] 3

Gene

Fmo3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Acts on TMA to produce TMA-N-oxide. Has activities of methimazole S-oxidation and NADPH oxidation associated with the N- or S-oxidation of trimethylamine and thioacetamide.

Catalytic activityi

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
N,N,N-trimethylamine + NADPH + O2 = N,N,N-trimethylamine N-oxide + NADP+ + H2O.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 13FADBy similarity5
Nucleotide bindingi40 – 41FADBy similarity2
Nucleotide bindingi60 – 61NADPBy similarity2
Nucleotide bindingi61 – 62FADBy similarity2
Nucleotide bindingi195 – 198NADPBy similarity4

GO - Molecular functioni

  • amino acid binding Source: RGD
  • flavin adenine dinucleotide binding Source: InterPro
  • monooxygenase activity Source: RGD
  • N,N-dimethylaniline monooxygenase activity Source: RGD
  • NADP binding Source: RGD
  • trimethylamine monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  • drug metabolic process Source: RGD

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.14.13.8 5301
SABIO-RKQ9EQ76

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 3 (EC:1.14.13.8)
Alternative name(s):
Dimethylaniline oxidase 3
Hepatic flavin-containing monooxygenase 3
Short name:
FMO 3
Trimethylamine monooxygenase (EC:1.14.13.148)
Gene namesi
Name:Fmo3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619761 Fmo3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei511 – 531HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476591 – 531Dimethylaniline monooxygenase [N-oxide-forming] 3Add BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9EQ76
PRIDEiQ9EQ76

PTM databases

CarbonylDBiQ9EQ76
iPTMnetiQ9EQ76
PhosphoSitePlusiQ9EQ76

Expressioni

Tissue specificityi

Detected in liver and kidney (at protein level) (PubMed:19307449). Expressed in kidney and liver. Weakly expressed in lung. Does not seem to be expressed in brain, adipose tissue, or muscle.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004864

Structurei

3D structure databases

ProteinModelPortaliQ9EQ76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FMO family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1399 Eukaryota
COG2072 LUCA
HOGENOMiHOG000076537
HOVERGENiHBG002037
InParanoidiQ9EQ76
KOiK00485
PhylomeDBiQ9EQ76
TreeFamiTF105285

Family and domain databases

Gene3Di3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR000960 Flavin_mOase
IPR020946 Flavin_mOase-like
IPR002255 Flavin_mOase_3
PfamiView protein in Pfam
PF00743 FMO-like, 1 hit
PIRSFiPIRSF000332 FMO, 1 hit
PRINTSiPR00370 FMOXYGENASE
PR01123 FMOXYGENASE3
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

Q9EQ76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKVAVIGA GVSGLAAIRS CLEEGLEPTC FERSDDVGGL WKFSDHTEEG
60 70 80 90 100
RASIYQSVFT NSSKEMMCFP DFPYPDDFPN FMHNSKLQEY ITSFATEKNL
110 120 130 140 150
LKYIQFETLV TRINKCPDFS TTGKWEVTTE KNSKKETAVF DAVMICSGHH
160 170 180 190 200
VYPHLPKDSF PGLNRFKGKC FHSRDYKEPG TWKGKRVLVI GLGNSGCDIA
210 220 230 240 250
AELSHVAQQV IISSRSGSWV MSRVWNDGYP WDMVVITRFQ TFLKNNLPTA
260 270 280 290 300
ISDWWYMKQM NARFKHENYG LMPLNGTLRK EPVFNDELPA RILCGTVSIK
310 320 330 340 350
PNVKEFTETS AVFEDGTVFE GIDCVIFATG YGYAYPFLDD SIIKSRNNEV
360 370 380 390 400
TLYKGIFPPQ LEKPTMAVIG LVQSLGAAIP TTDLQARWAA QVIRGTCILP
410 420 430 440 450
SVNDMMDDID EKMGKKLKWF GNSTTIQTDY IVYMDELASF IGAKPNILWL
460 470 480 490 500
FLKDPRLAIE VFFGPCSPYQ FRLVGPGKWS GARNAILTQW DRSLKPMKTR
510 520 530
VVGGIQKPCL YSHFLRLLAV PVLIALFLVL I
Length:531
Mass (Da):59,960
Last modified:March 1, 2001 - v1
Checksum:i35A89988323B311F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti501V → D in AAH87008 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286595 mRNA Translation: AAG44891.1
BC087008 mRNA Translation: AAH87008.1
RefSeqiNP_445885.2, NM_053433.2
UniGeneiRn.163228

Genome annotation databases

GeneIDi84493
KEGGirno:84493
UCSCiRGD:619761 rat

Similar proteinsi

Entry informationi

Entry nameiFMO3_RAT
AccessioniPrimary (citable) accession number: Q9EQ76
Secondary accession number(s): Q5PQV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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