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Protein

Pescadillo homolog

Gene

Pes1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation2 Publications

GO - Molecular functioni

  1. poly(A) RNA binding Source: MGI
  2. ribonucleoprotein complex binding Source: UniProtKB-HAMAP
  3. RNA binding Source: GO_Central

GO - Biological processi

  1. cell proliferation Source: UniProtKB
  2. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  3. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  4. nucleolus organization Source: MGI
  5. protein localization to organelle Source: MGI
  6. regulation of cell cycle Source: MGI
  7. ribosome biogenesis Source: MGI
  8. rRNA processing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Pescadillo homologUniRule annotation
Gene namesi
Name:Pes1
Synonyms:Pes
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1890613. Pes1.

Subcellular locationi

Nucleusnucleolus. Nucleusnucleoplasm. Chromosome
Note: Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.

GO - Cellular componenti

  1. condensed chromosome Source: MGI
  2. cytoplasm Source: MGI
  3. membrane Source: MGI
  4. nucleolus Source: UniProtKB
  5. nucleoplasm Source: UniProtKB
  6. nucleus Source: MGI
  7. PeBoW complex Source: UniProtKB
  8. preribosome, large subunit precursor Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryos die during preimplantation stages of development, with blastomeres failing to progress past morula stages. Within blastocysts the nucleoli fail to form correctly and the number of ribosomes appears dramatically reduced.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Pescadillo homologPRO_0000186189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei98 – 981N6-acetyllysineBy similarity

Post-translational modificationi

Sumoylated.UniRule annotation

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

MaxQBiQ9EQ61.
PaxDbiQ9EQ61.
PRIDEiQ9EQ61.

PTM databases

PhosphoSiteiQ9EQ61.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels appear to be found in tissues that contain a population of proliferating cells, such as ovary and testis. Also appears to be highly expressed in kidney and liver. In the brain expression is restricted to neural progenitor cells and postmitotic neurons. Highly expressed in malignant astrocytes.2 Publications

Developmental stagei

In 2-cell and 4-cell stage interphase blastomeres expression is restricted to a sub-nuclear band that encircles one or more large vacuoles within the nucleus. These vacuoles may give rise to the mature nucleolus. Later in embryogenesis high levels are detected in developing liver. Is also widely and highly expressed throughout the developing brain and spinal cord at embryonic day 13.2 Publications

Inductioni

Induced in malignant astrocytes following the loss of p53. Induced in hepatocytes following partial hepatectomy.2 Publications

Gene expression databases

BgeeiQ9EQ61.
ExpressionAtlasiQ9EQ61. baseline and differential.
GenevestigatoriQ9EQ61.

Interactioni

Subunit structurei

Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Interacts with IRS1 and UBTF. May interact with MAP1B.UniRule annotation3 Publications

Protein-protein interaction databases

BioGridi211118. 1 interaction.
IntActiQ9EQ61. 1 interaction.
MINTiMINT-4107198.

Structurei

3D structure databases

ProteinModelPortaliQ9EQ61.
SMRiQ9EQ61. Positions 321-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini321 – 41494BRCTUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 257257Sufficient for nucleolar localizationBy similarityAdd
BLAST
Regioni1 – 5454Required for 28S ribosomal RNA processingBy similarityAdd
BLAST
Regioni312 – 414103Sufficient for interaction with MAP1BAdd
BLAST
Regioni535 – 58450Required for 28S ribosomal RNA processingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi454 – 52774Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the pescadillo family.UniRule annotation
Contains 1 BRCT domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG5163.
GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
HOVERGENiHBG023409.
InParanoidiQ9EQ61.
KOiK14843.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo.
InterProiIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQ61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGLEKKKYE RGSATNYITR NKARKKLQLS LPDFRRLCIL KGIYPHEPKH
60 70 80 90 100
KKKVNKGSTA ARTFYLIKDI KFLLHEPIVN KFREYKVFVR KLRKAYGKSE
110 120 130 140 150
WNAVERLKDN KPCYKLDHIV KERYPTFIDA LRDLDDALSM CFLFSTFPRT
160 170 180 190 200
GKCHVQTIQL CRRLTVEFLH YVITARALRK VFLSIKGIYY QAEVLGQPIV
210 220 230 240 250
WIAPYAFSHD HPTDVDYRVM ATFTEFYTTL LGFVNFRLYQ SLNLHYPPKL
260 270 280 290 300
EGQAQAETKI SEDTYALDSE SSMEKLAALS ASLARVVVPA IEEAEADEFP
310 320 330 340 350
TDGEVTAQEE DRKKELEAQE KHKKLFEGLK FFLNREVPRE ALAFIIRSFG
360 370 380 390 400
GDVSWDKSLC IGATYDVTDS CITHQIVDRP GQQTPIIGRY YVQPQWVFDC
410 420 430 440 450
VNARLLLPVA EYFPGMQLPP HLSPFVSEKE GDYIPPEKLK LLALQRGEDP
460 470 480 490 500
GHLEEEEEED EDDDNEGDVA AENEEEDVEV ESEEEEEEEV HLSALEQHRL
510 520 530 540 550
EEKKPQVMAG TVKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG
560 570 580
KRRKIREANK LAEKRKAHDD AVRSEKKAKR TRPV
Length:584
Mass (Da):67,796
Last modified:March 1, 2001 - v1
Checksum:i63D3C352DF5E9893
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF289539 mRNA. Translation: AAG40734.1.
AK029075 mRNA. Translation: BAC26278.1.
AK088831 mRNA. Translation: BAC40599.1.
BC004844 mRNA. Translation: AAH04844.1.
BC011142 mRNA. Translation: AAH11142.1.
CCDSiCCDS24372.1.
RefSeqiNP_075027.1. NM_022889.3.
UniGeneiMm.28659.

Genome annotation databases

EnsembliENSMUST00000020705; ENSMUSP00000020705; ENSMUSG00000020430.
GeneIDi64934.
KEGGimmu:64934.
UCSCiuc007hua.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF289539 mRNA. Translation: AAG40734.1.
AK029075 mRNA. Translation: BAC26278.1.
AK088831 mRNA. Translation: BAC40599.1.
BC004844 mRNA. Translation: AAH04844.1.
BC011142 mRNA. Translation: AAH11142.1.
CCDSiCCDS24372.1.
RefSeqiNP_075027.1. NM_022889.3.
UniGeneiMm.28659.

3D structure databases

ProteinModelPortaliQ9EQ61.
SMRiQ9EQ61. Positions 321-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211118. 1 interaction.
IntActiQ9EQ61. 1 interaction.
MINTiMINT-4107198.

PTM databases

PhosphoSiteiQ9EQ61.

Proteomic databases

MaxQBiQ9EQ61.
PaxDbiQ9EQ61.
PRIDEiQ9EQ61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020705; ENSMUSP00000020705; ENSMUSG00000020430.
GeneIDi64934.
KEGGimmu:64934.
UCSCiuc007hua.1. mouse.

Organism-specific databases

CTDi23481.
MGIiMGI:1890613. Pes1.

Phylogenomic databases

eggNOGiCOG5163.
GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
HOVERGENiHBG023409.
InParanoidiQ9EQ61.
KOiK14843.

Miscellaneous databases

NextBioi320241.
PROiQ9EQ61.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EQ61.
ExpressionAtlasiQ9EQ61. baseline and differential.
GenevestigatoriQ9EQ61.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo.
InterProiIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The murine Pes1 gene encodes a nuclear protein containing a BRCT domain."
    Haque J., Boger S., Li J., Duncan S.A.
    Genomics 70:201-210(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Skin and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon and Mammary gland.
  4. Cited for: INDUCTION, DEVELOPMENTAL STAGE.
  5. "Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation."
    Lerch-Gaggl A.F., Haque J., Li J., Ning G., Traktman P., Duncan S.A.
    J. Biol. Chem. 277:45347-45355(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE.
  6. "Physical and functional interaction between Pes1 and Bop1 in mammalian ribosome biogenesis."
    Lapik Y.R., Fernandes C.J., Lau L.F., Pestov D.G.
    Mol. Cell 15:17-29(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BOP1, SUBCELLULAR LOCATION.
  7. "Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells."
    Prisco M., Maiorana A., Guerzoni C., Calin G., Calabretta B., Voit R., Grummt I., Baserga R.
    Mol. Cell. Biol. 24:5421-5433(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IRS1 AND UBTF, SUBCELLULAR LOCATION.
  8. "Light chain 1 of microtubule-associated protein 1B can negatively regulate the action of Pes1."
    Lerch-Gaggl A.F., Sun K., Duncan S.A.
    J. Biol. Chem. 282:11308-11316(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MAP1B, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPESC_MOUSE
AccessioniPrimary (citable) accession number: Q9EQ61
Secondary accession number(s): Q542F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: March 1, 2001
Last modified: March 4, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.