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Protein

Pescadillo homolog

Gene

Pes1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  • nucleolus organization Source: MGI
  • protein localization to organelle Source: MGI
  • regulation of cell cycle Source: MGI
  • ribosome biogenesis Source: MGI
  • rRNA processing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

ReactomeiR-MMU-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Pescadillo homologUniRule annotation
Gene namesi
Name:Pes1
Synonyms:Pes
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1890613. Pes1.

Subcellular locationi

  • Nucleusnucleolus
  • Nucleusnucleoplasm
  • Chromosome

  • Note: Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.

GO - Cellular componenti

  • condensed chromosome Source: MGI
  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • nucleus Source: MGI
  • PeBoW complex Source: UniProtKB
  • preribosome, large subunit precursor Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryos die during preimplantation stages of development, with blastomeres failing to progress past morula stages. Within blastocysts the nucleoli fail to form correctly and the number of ribosomes appears dramatically reduced.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001861891 – 584Pescadillo homologAdd BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei98N6-acetyllysineBy similarity1
Cross-linki513Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki513Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Sumoylated.UniRule annotation

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9EQ61.
MaxQBiQ9EQ61.
PaxDbiQ9EQ61.
PRIDEiQ9EQ61.

PTM databases

iPTMnetiQ9EQ61.
PhosphoSitePlusiQ9EQ61.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels appear to be found in tissues that contain a population of proliferating cells, such as ovary and testis. Also appears to be highly expressed in kidney and liver. In the brain expression is restricted to neural progenitor cells and postmitotic neurons. Highly expressed in malignant astrocytes.2 Publications

Developmental stagei

In 2-cell and 4-cell stage interphase blastomeres expression is restricted to a sub-nuclear band that encircles one or more large vacuoles within the nucleus. These vacuoles may give rise to the mature nucleolus. Later in embryogenesis high levels are detected in developing liver. Is also widely and highly expressed throughout the developing brain and spinal cord at embryonic day 13.2 Publications

Inductioni

Induced in malignant astrocytes following the loss of p53. Induced in hepatocytes following partial hepatectomy.2 Publications

Gene expression databases

BgeeiENSMUSG00000020430.
ExpressionAtlasiQ9EQ61. baseline and differential.
GenevisibleiQ9EQ61. MM.

Interactioni

Subunit structurei

Component of the PeBoW complex, composed of BOP1, PES1 and WDR12 (PubMed:15225545). The complex is held together by BOP1, which interacts with PES1 via its N-terminal domain and with WDR12 via a high-affinity interaction between the seven-bladed beta-propeller domains of the 2 proteins. The PeBoW complex associates with the 66S pre-ribosome (By similarity). The PeBoW complex also associates with DDX27, PES1 interacts directly with DDX27 (By similarity). Interacts with IRS1 and UBTF (PubMed:15169904). May interact with MAP1B (PubMed:17308336).UniRule annotation3 Publications

Protein-protein interaction databases

BioGridi211118. 1 interactor.
IntActiQ9EQ61. 1 interactor.
MINTiMINT-4107198.
STRINGi10090.ENSMUSP00000020705.

Structurei

3D structure databases

ProteinModelPortaliQ9EQ61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini321 – 414BRCTUniRule annotationAdd BLAST94

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 257Sufficient for nucleolar localizationBy similarityAdd BLAST257
Regioni1 – 54Required for 28S ribosomal RNA processingBy similarityAdd BLAST54
Regioni312 – 414Sufficient for interaction with MAP1B1 PublicationAdd BLAST103
Regioni535 – 584Required for 28S ribosomal RNA processingBy similarityAdd BLAST50

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi454 – 527Glu-richAdd BLAST74

Sequence similaritiesi

Belongs to the pescadillo family.UniRule annotation
Contains 1 BRCT domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG2481. Eukaryota.
COG5163. LUCA.
GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
HOVERGENiHBG023409.
InParanoidiQ9EQ61.
KOiK14843.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQ61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGLEKKKYE RGSATNYITR NKARKKLQLS LPDFRRLCIL KGIYPHEPKH
60 70 80 90 100
KKKVNKGSTA ARTFYLIKDI KFLLHEPIVN KFREYKVFVR KLRKAYGKSE
110 120 130 140 150
WNAVERLKDN KPCYKLDHIV KERYPTFIDA LRDLDDALSM CFLFSTFPRT
160 170 180 190 200
GKCHVQTIQL CRRLTVEFLH YVITARALRK VFLSIKGIYY QAEVLGQPIV
210 220 230 240 250
WIAPYAFSHD HPTDVDYRVM ATFTEFYTTL LGFVNFRLYQ SLNLHYPPKL
260 270 280 290 300
EGQAQAETKI SEDTYALDSE SSMEKLAALS ASLARVVVPA IEEAEADEFP
310 320 330 340 350
TDGEVTAQEE DRKKELEAQE KHKKLFEGLK FFLNREVPRE ALAFIIRSFG
360 370 380 390 400
GDVSWDKSLC IGATYDVTDS CITHQIVDRP GQQTPIIGRY YVQPQWVFDC
410 420 430 440 450
VNARLLLPVA EYFPGMQLPP HLSPFVSEKE GDYIPPEKLK LLALQRGEDP
460 470 480 490 500
GHLEEEEEED EDDDNEGDVA AENEEEDVEV ESEEEEEEEV HLSALEQHRL
510 520 530 540 550
EEKKPQVMAG TVKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG
560 570 580
KRRKIREANK LAEKRKAHDD AVRSEKKAKR TRPV
Length:584
Mass (Da):67,796
Last modified:March 1, 2001 - v1
Checksum:i63D3C352DF5E9893
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF289539 mRNA. Translation: AAG40734.1.
AK029075 mRNA. Translation: BAC26278.1.
AK088831 mRNA. Translation: BAC40599.1.
BC004844 mRNA. Translation: AAH04844.1.
BC011142 mRNA. Translation: AAH11142.1.
CCDSiCCDS24372.1.
RefSeqiNP_075027.1. NM_022889.3.
UniGeneiMm.28659.

Genome annotation databases

EnsembliENSMUST00000020705; ENSMUSP00000020705; ENSMUSG00000020430.
GeneIDi64934.
KEGGimmu:64934.
UCSCiuc007hua.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF289539 mRNA. Translation: AAG40734.1.
AK029075 mRNA. Translation: BAC26278.1.
AK088831 mRNA. Translation: BAC40599.1.
BC004844 mRNA. Translation: AAH04844.1.
BC011142 mRNA. Translation: AAH11142.1.
CCDSiCCDS24372.1.
RefSeqiNP_075027.1. NM_022889.3.
UniGeneiMm.28659.

3D structure databases

ProteinModelPortaliQ9EQ61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211118. 1 interactor.
IntActiQ9EQ61. 1 interactor.
MINTiMINT-4107198.
STRINGi10090.ENSMUSP00000020705.

PTM databases

iPTMnetiQ9EQ61.
PhosphoSitePlusiQ9EQ61.

Proteomic databases

EPDiQ9EQ61.
MaxQBiQ9EQ61.
PaxDbiQ9EQ61.
PRIDEiQ9EQ61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020705; ENSMUSP00000020705; ENSMUSG00000020430.
GeneIDi64934.
KEGGimmu:64934.
UCSCiuc007hua.1. mouse.

Organism-specific databases

CTDi23481.
MGIiMGI:1890613. Pes1.

Phylogenomic databases

eggNOGiKOG2481. Eukaryota.
COG5163. LUCA.
GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
HOVERGENiHBG023409.
InParanoidiQ9EQ61.
KOiK14843.

Enzyme and pathway databases

ReactomeiR-MMU-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

PROiQ9EQ61.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020430.
ExpressionAtlasiQ9EQ61. baseline and differential.
GenevisibleiQ9EQ61. MM.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPESC_MOUSE
AccessioniPrimary (citable) accession number: Q9EQ61
Secondary accession number(s): Q542F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.