Q9EQ61 (PESC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pescadillo homolog

Gene names

Name:	Pes1
Synonyms:	Pes

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.6 Ref.8
Subunit structure	Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Interacts with IRS1 and UBTF. May interact with MAP1B. Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus › nucleolus. Nucleus › nucleoplasm. Chromosome. Note: Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8
Tissue specificity	Ubiquitous. Highest levels appear to be found in tissues that contain a population of proliferating cells, such as ovary and testis. Also appears to be highly expressed in kidney and liver. In the brain expression is restricted to neural progenitor cells and postmitotic neurons. Highly expressed in malignant astrocytes. Ref.1 Ref.5
Developmental stage	In 2-cell and 4-cell stage interphase blastomeres expression is restricted to a sub-nuclear band that encircles one or more large vacuoles within the nucleus. These vacuoles may give rise to the mature nucleolus. Later in embryogenesis high levels are detected in developing liver. Is also widely and highly expressed throughout the developing brain and spinal cord at embryonic day 13. Ref.4 Ref.5
Induction	Induced in malignant astrocytes following the loss of p53. Induced in hepatocytes following partial hepatectomy. Ref.4 Ref.5
Post-translational modification	Sumoylated By similarity. HAMAP-Rule MF_03028
Disruption phenotype	Embryos die during preimplantation stages of development, with blastomeres failing to progress past morula stages. Within blastocysts the nucleoli fail to form correctly and the number of ribosomes appears dramatically reduced. Ref.5
Sequence similarities	Belongs to the pescadillo family. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Chromosome Nucleus
PTM	Acetylation Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from sequence or structural similarity. Source: UniProtKB maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from sequence or structural similarity. Source: UniProtKB nucleolus organization Inferred from mutant phenotype Ref.5. Source: MGI protein localization to organelle Inferred from mutant phenotype Ref.6. Source: MGI rRNA processing Inferred from mutant phenotype Ref.6. Source: MGI regulation of cell cycle Inferred from electronic annotation. Source: Ensembl ribosome biogenesis Inferred from mutant phenotype Ref.5. Source: MGI
Cellular_component	PeBoW complex Inferred from sequence or structural similarity. Source: UniProtKB condensed chromosome Inferred from direct assay Ref.5. Source: MGI nucleolus Inferred from sequence or structural similarity. Source: UniProtKB nucleoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from direct assay Ref.1. Source: MGI preribosome, large subunit precursor Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein binding Inferred from physical interaction Ref.6Ref.8. Source: MGI ribonucleoprotein complex binding Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Pescadillo homolog HAMAP-Rule MF_03028		PRO_0000186189
Regions
	Domain	321 – 414	94	BRCT
	Region	1 – 257	257	Sufficient for nucleolar localization By similarity
	Region	1 – 54	54	Required for 28S ribosomal RNA processing By similarity
	Region	312 – 414	103	Sufficient for interaction with MAP1B HAMAP-Rule MF_03028
	Region	535 – 584	50	Required for 28S ribosomal RNA processing By similarity
	Compositional bias	454 – 527	74	Glu-rich HAMAP-Rule MF_03028
Amino acid modifications
	Modified residue	98	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9EQ61 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 63D3C352DF5E9893	FASTA	584	67,796
	10 20 30 40 50 60 MGGLEKKKYE RGSATNYITR NKARKKLQLS LPDFRRLCIL KGIYPHEPKH KKKVNKGSTA 70 80 90 100 110 120 ARTFYLIKDI KFLLHEPIVN KFREYKVFVR KLRKAYGKSE WNAVERLKDN KPCYKLDHIV 130 140 150 160 170 180 KERYPTFIDA LRDLDDALSM CFLFSTFPRT GKCHVQTIQL CRRLTVEFLH YVITARALRK 190 200 210 220 230 240 VFLSIKGIYY QAEVLGQPIV WIAPYAFSHD HPTDVDYRVM ATFTEFYTTL LGFVNFRLYQ 250 260 270 280 290 300 SLNLHYPPKL EGQAQAETKI SEDTYALDSE SSMEKLAALS ASLARVVVPA IEEAEADEFP 310 320 330 340 350 360 TDGEVTAQEE DRKKELEAQE KHKKLFEGLK FFLNREVPRE ALAFIIRSFG GDVSWDKSLC 370 380 390 400 410 420 IGATYDVTDS CITHQIVDRP GQQTPIIGRY YVQPQWVFDC VNARLLLPVA EYFPGMQLPP 430 440 450 460 470 480 HLSPFVSEKE GDYIPPEKLK LLALQRGEDP GHLEEEEEED EDDDNEGDVA AENEEEDVEV 490 500 510 520 530 540 ESEEEEEEEV HLSALEQHRL EEKKPQVMAG TVKLEDKQRL AQEEESEAKR LAIMMMKKRE 550 560 570 580 KYLYQKIMFG KRRKIREANK LAEKRKAHDD AVRSEKKAKR TRPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine Pes1 gene encodes a nuclear protein containing a BRCT domain." Haque J., Boger S., Li J., Duncan S.A. Genomics 70:201-210(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Skin and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Mammary gland.
[4]	"Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells." Kinoshita Y., Jarell A.D., Flaman J.-M., Foltz G., Schuster J., Sopher B.L., Irvin D.K., Kanning K., Kornblum H.I., Nelson P.S., Hieter P., Morrison R.S. J. Biol. Chem. 276:6656-6665(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, DEVELOPMENTAL STAGE.
[5]	"Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation." Lerch-Gaggl A.F., Haque J., Li J., Ning G., Traktman P., Duncan S.A. J. Biol. Chem. 277:45347-45355(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE.
[6]	"Physical and functional interaction between Pes1 and Bop1 in mammalian ribosome biogenesis." Lapik Y.R., Fernandes C.J., Lau L.F., Pestov D.G. Mol. Cell 15:17-29(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH BOP1, SUBCELLULAR LOCATION.
[7]	"Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells." Prisco M., Maiorana A., Guerzoni C., Calin G., Calabretta B., Voit R., Grummt I., Baserga R. Mol. Cell. Biol. 24:5421-5433(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IRS1 AND UBTF, SUBCELLULAR LOCATION.
[8]	"Light chain 1 of microtubule-associated protein 1B can negatively regulate the action of Pes1." Lerch-Gaggl A.F., Sun K., Duncan S.A. J. Biol. Chem. 282:11308-11316(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MAP1B, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF289539 mRNA. Translation: AAG40734.1. AK029075 mRNA. Translation: BAC26278.1. AK088831 mRNA. Translation: BAC40599.1. BC004844 mRNA. Translation: AAH04844.1. BC011142 mRNA. Translation: AAH11142.1.
CCDS	CCDS24372.1.
RefSeq	NP_075027.1. NM_022889.3.
UniGene	Mm.28659.
3D structure databases
ProteinModelPortal	Q9EQ61.
SMR	Q9EQ61. Positions 321-413.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	211118. 1 interaction.
IntAct	Q9EQ61. 1 interaction.
MINT	MINT-4107198.
PTM databases
PhosphoSite	Q9EQ61.
Proteomic databases
PaxDb	Q9EQ61.
PRIDE	Q9EQ61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020705; ENSMUSP00000020705; ENSMUSG00000020430.
GeneID	64934.
KEGG	mmu:64934.
UCSC	uc007hua.1. mouse.
Organism-specific databases
CTD	23481.
MGI	MGI:1890613. Pes1.
Phylogenomic databases
eggNOG	COG5163.
GeneTree	ENSGT00390000002626.
HOGENOM	HOG000006022.
HOVERGEN	HBG023409.
KO	K14843.
Gene expression databases
ArrayExpress	Q9EQ61.
Bgee	Q9EQ61.
Genevestigator	Q9EQ61.
Family and domain databases
Gene3D	3.40.50.10190. 1 hit.
HAMAP	MF_03028. Pescadillo.
InterPro	IPR001357. BRCT_dom. IPR010613. PES. [Graphical view]
Pfam	PF00533. BRCT. 1 hit. PF06732. Pescadillo_N. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. [Graphical view]
SUPFAM	SSF52113. SSF52113. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	320241.
PRO	Q9EQ61.
SOURCE	Search...

Entry information

Entry name

PESC_MOUSE

Accession

Primary (citable) accession number: Q9EQ61
Secondary accession number(s): Q542F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	March 1, 2001
Last modified:	July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program