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Q9EQ61 (PESC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pescadillo homolog
Gene names
Name:Pes1
Synonyms:Pes
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.6 Ref.8

Subunit structure

Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Interacts with IRS1 and UBTF. May interact with MAP1B. Ref.6 Ref.7 Ref.8

Subcellular location

Nucleusnucleolus. Nucleusnucleoplasm. Chromosome. Note: Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8

Tissue specificity

Ubiquitous. Highest levels appear to be found in tissues that contain a population of proliferating cells, such as ovary and testis. Also appears to be highly expressed in kidney and liver. In the brain expression is restricted to neural progenitor cells and postmitotic neurons. Highly expressed in malignant astrocytes. Ref.1 Ref.5

Developmental stage

In 2-cell and 4-cell stage interphase blastomeres expression is restricted to a sub-nuclear band that encircles one or more large vacuoles within the nucleus. These vacuoles may give rise to the mature nucleolus. Later in embryogenesis high levels are detected in developing liver. Is also widely and highly expressed throughout the developing brain and spinal cord at embryonic day 13. Ref.4 Ref.5

Induction

Induced in malignant astrocytes following the loss of p53. Induced in hepatocytes following partial hepatectomy. Ref.4 Ref.5

Post-translational modification

Sumoylated By similarity. HAMAP-Rule MF_03028

Disruption phenotype

Embryos die during preimplantation stages of development, with blastomeres failing to progress past morula stages. Within blastocysts the nucleoli fail to form correctly and the number of ribosomes appears dramatically reduced. Ref.5

Sequence similarities

Belongs to the pescadillo family.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processRibosome biogenesis
rRNA processing
   Cellular componentChromosome
Nucleus
   PTMAcetylation
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from sequence or structural similarity. Source: UniProtKB

maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from sequence or structural similarity. Source: UniProtKB

nucleolus organization

Inferred from mutant phenotype Ref.5. Source: MGI

protein localization to organelle

Inferred from mutant phenotype Ref.6. Source: MGI

rRNA processing

Inferred from mutant phenotype Ref.6. Source: MGI

regulation of cell cycle

Inferred from electronic annotation. Source: Ensembl

ribosome biogenesis

Inferred from mutant phenotype Ref.5. Source: MGI

   Cellular_componentPeBoW complex

Inferred from sequence or structural similarity. Source: UniProtKB

condensed chromosome

Inferred from direct assay Ref.5. Source: MGI

nucleolus

Inferred from sequence or structural similarity. Source: UniProtKB

nucleoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from direct assay Ref.1. Source: MGI

preribosome, large subunit precursor

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction Ref.6Ref.8. Source: MGI

ribonucleoprotein complex binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 584584Pescadillo homolog HAMAP-Rule MF_03028
PRO_0000186189

Regions

Domain321 – 41494BRCT
Region1 – 257257Sufficient for nucleolar localization By similarity
Region1 – 5454Required for 28S ribosomal RNA processing By similarity
Region312 – 414103Sufficient for interaction with MAP1B HAMAP-Rule MF_03028
Region535 – 58450Required for 28S ribosomal RNA processing By similarity
Compositional bias454 – 52774Glu-rich HAMAP-Rule MF_03028

Amino acid modifications

Modified residue981N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9EQ61 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 63D3C352DF5E9893

FASTA58467,796
        10         20         30         40         50         60 
MGGLEKKKYE RGSATNYITR NKARKKLQLS LPDFRRLCIL KGIYPHEPKH KKKVNKGSTA 

        70         80         90        100        110        120 
ARTFYLIKDI KFLLHEPIVN KFREYKVFVR KLRKAYGKSE WNAVERLKDN KPCYKLDHIV 

       130        140        150        160        170        180 
KERYPTFIDA LRDLDDALSM CFLFSTFPRT GKCHVQTIQL CRRLTVEFLH YVITARALRK 

       190        200        210        220        230        240 
VFLSIKGIYY QAEVLGQPIV WIAPYAFSHD HPTDVDYRVM ATFTEFYTTL LGFVNFRLYQ 

       250        260        270        280        290        300 
SLNLHYPPKL EGQAQAETKI SEDTYALDSE SSMEKLAALS ASLARVVVPA IEEAEADEFP 

       310        320        330        340        350        360 
TDGEVTAQEE DRKKELEAQE KHKKLFEGLK FFLNREVPRE ALAFIIRSFG GDVSWDKSLC 

       370        380        390        400        410        420 
IGATYDVTDS CITHQIVDRP GQQTPIIGRY YVQPQWVFDC VNARLLLPVA EYFPGMQLPP 

       430        440        450        460        470        480 
HLSPFVSEKE GDYIPPEKLK LLALQRGEDP GHLEEEEEED EDDDNEGDVA AENEEEDVEV 

       490        500        510        520        530        540 
ESEEEEEEEV HLSALEQHRL EEKKPQVMAG TVKLEDKQRL AQEEESEAKR LAIMMMKKRE 

       550        560        570        580 
KYLYQKIMFG KRRKIREANK LAEKRKAHDD AVRSEKKAKR TRPV 

« Hide

References

« Hide 'large scale' references
[1]"The murine Pes1 gene encodes a nuclear protein containing a BRCT domain."
Haque J., Boger S., Li J., Duncan S.A.
Genomics 70:201-210(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Skin and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon and Mammary gland.
[4]"Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells."
Kinoshita Y., Jarell A.D., Flaman J.-M., Foltz G., Schuster J., Sopher B.L., Irvin D.K., Kanning K., Kornblum H.I., Nelson P.S., Hieter P., Morrison R.S.
J. Biol. Chem. 276:6656-6665(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, DEVELOPMENTAL STAGE.
[5]"Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation."
Lerch-Gaggl A.F., Haque J., Li J., Ning G., Traktman P., Duncan S.A.
J. Biol. Chem. 277:45347-45355(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE.
[6]"Physical and functional interaction between Pes1 and Bop1 in mammalian ribosome biogenesis."
Lapik Y.R., Fernandes C.J., Lau L.F., Pestov D.G.
Mol. Cell 15:17-29(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BOP1, SUBCELLULAR LOCATION.
[7]"Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells."
Prisco M., Maiorana A., Guerzoni C., Calin G., Calabretta B., Voit R., Grummt I., Baserga R.
Mol. Cell. Biol. 24:5421-5433(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IRS1 AND UBTF, SUBCELLULAR LOCATION.
[8]"Light chain 1 of microtubule-associated protein 1B can negatively regulate the action of Pes1."
Lerch-Gaggl A.F., Sun K., Duncan S.A.
J. Biol. Chem. 282:11308-11316(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MAP1B, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF289539 mRNA. Translation: AAG40734.1.
AK029075 mRNA. Translation: BAC26278.1.
AK088831 mRNA. Translation: BAC40599.1.
BC004844 mRNA. Translation: AAH04844.1.
BC011142 mRNA. Translation: AAH11142.1.
CCDSCCDS24372.1.
RefSeqNP_075027.1. NM_022889.3.
UniGeneMm.28659.

3D structure databases

ProteinModelPortalQ9EQ61.
SMRQ9EQ61. Positions 321-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid211118. 1 interaction.
IntActQ9EQ61. 1 interaction.
MINTMINT-4107198.

PTM databases

PhosphoSiteQ9EQ61.

Proteomic databases

PaxDbQ9EQ61.
PRIDEQ9EQ61.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020705; ENSMUSP00000020705; ENSMUSG00000020430.
GeneID64934.
KEGGmmu:64934.
UCSCuc007hua.1. mouse.

Organism-specific databases

CTD23481.
MGIMGI:1890613. Pes1.

Phylogenomic databases

eggNOGCOG5163.
GeneTreeENSGT00390000002626.
HOGENOMHOG000006022.
HOVERGENHBG023409.
KOK14843.

Gene expression databases

ArrayExpressQ9EQ61.
BgeeQ9EQ61.
GenevestigatorQ9EQ61.

Family and domain databases

Gene3D3.40.50.10190. 1 hit.
HAMAPMF_03028. Pescadillo.
InterProIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMSSF52113. SSF52113. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio320241.
PROQ9EQ61.
SOURCESearch...

Entry information

Entry namePESC_MOUSE
AccessionPrimary (citable) accession number: Q9EQ61
Secondary accession number(s): Q542F0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot