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Q9EQ61

- PESC_MOUSE

UniProt

Q9EQ61 - PESC_MOUSE

Protein

Pescadillo homolog

Gene

Pes1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.2 PublicationsUniRule annotation

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. ribonucleoprotein complex binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell proliferation Source: UniProtKB
    2. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
    3. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
    4. nucleolus organization Source: MGI
    5. protein localization to organelle Source: MGI
    6. regulation of cell cycle Source: Ensembl
    7. ribosome biogenesis Source: MGI
    8. rRNA processing Source: MGI

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pescadillo homologUniRule annotation
    Gene namesi
    Name:Pes1
    Synonyms:Pes
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1890613. Pes1.

    Subcellular locationi

    Nucleusnucleolus. Nucleusnucleoplasm. Chromosome
    Note: Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.

    GO - Cellular componenti

    1. condensed chromosome Source: MGI
    2. nucleolus Source: UniProtKB
    3. nucleoplasm Source: UniProtKB
    4. nucleus Source: MGI
    5. PeBoW complex Source: UniProtKB
    6. preribosome, large subunit precursor Source: UniProtKB

    Keywords - Cellular componenti

    Chromosome, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Embryos die during preimplantation stages of development, with blastomeres failing to progress past morula stages. Within blastocysts the nucleoli fail to form correctly and the number of ribosomes appears dramatically reduced.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Pescadillo homologPRO_0000186189Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei98 – 981N6-acetyllysineBy similarity

    Post-translational modificationi

    Sumoylated.UniRule annotation

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    PaxDbiQ9EQ61.
    PRIDEiQ9EQ61.

    PTM databases

    PhosphoSiteiQ9EQ61.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highest levels appear to be found in tissues that contain a population of proliferating cells, such as ovary and testis. Also appears to be highly expressed in kidney and liver. In the brain expression is restricted to neural progenitor cells and postmitotic neurons. Highly expressed in malignant astrocytes.2 Publications

    Developmental stagei

    In 2-cell and 4-cell stage interphase blastomeres expression is restricted to a sub-nuclear band that encircles one or more large vacuoles within the nucleus. These vacuoles may give rise to the mature nucleolus. Later in embryogenesis high levels are detected in developing liver. Is also widely and highly expressed throughout the developing brain and spinal cord at embryonic day 13.2 Publications

    Inductioni

    Induced in malignant astrocytes following the loss of p53. Induced in hepatocytes following partial hepatectomy.2 Publications

    Gene expression databases

    ArrayExpressiQ9EQ61.
    BgeeiQ9EQ61.
    GenevestigatoriQ9EQ61.

    Interactioni

    Subunit structurei

    Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Interacts with IRS1 and UBTF. May interact with MAP1B.3 PublicationsUniRule annotation

    Protein-protein interaction databases

    BioGridi211118. 1 interaction.
    IntActiQ9EQ61. 1 interaction.
    MINTiMINT-4107198.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EQ61.
    SMRiQ9EQ61. Positions 321-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini321 – 41494BRCTUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 257257Sufficient for nucleolar localizationBy similarityAdd
    BLAST
    Regioni1 – 5454Required for 28S ribosomal RNA processingBy similarityAdd
    BLAST
    Regioni312 – 414103Sufficient for interaction with MAP1BAdd
    BLAST
    Regioni535 – 58450Required for 28S ribosomal RNA processingBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi454 – 52774Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the pescadillo family.UniRule annotation
    Contains 1 BRCT domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG5163.
    GeneTreeiENSGT00390000002626.
    HOGENOMiHOG000006022.
    HOVERGENiHBG023409.
    KOiK14843.

    Family and domain databases

    Gene3Di3.40.50.10190. 1 hit.
    HAMAPiMF_03028. Pescadillo.
    InterProiIPR001357. BRCT_dom.
    IPR010613. PES.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF06732. Pescadillo_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 1 hit.
    [Graphical view]
    SUPFAMiSSF52113. SSF52113. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EQ61-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGGLEKKKYE RGSATNYITR NKARKKLQLS LPDFRRLCIL KGIYPHEPKH    50
    KKKVNKGSTA ARTFYLIKDI KFLLHEPIVN KFREYKVFVR KLRKAYGKSE 100
    WNAVERLKDN KPCYKLDHIV KERYPTFIDA LRDLDDALSM CFLFSTFPRT 150
    GKCHVQTIQL CRRLTVEFLH YVITARALRK VFLSIKGIYY QAEVLGQPIV 200
    WIAPYAFSHD HPTDVDYRVM ATFTEFYTTL LGFVNFRLYQ SLNLHYPPKL 250
    EGQAQAETKI SEDTYALDSE SSMEKLAALS ASLARVVVPA IEEAEADEFP 300
    TDGEVTAQEE DRKKELEAQE KHKKLFEGLK FFLNREVPRE ALAFIIRSFG 350
    GDVSWDKSLC IGATYDVTDS CITHQIVDRP GQQTPIIGRY YVQPQWVFDC 400
    VNARLLLPVA EYFPGMQLPP HLSPFVSEKE GDYIPPEKLK LLALQRGEDP 450
    GHLEEEEEED EDDDNEGDVA AENEEEDVEV ESEEEEEEEV HLSALEQHRL 500
    EEKKPQVMAG TVKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG 550
    KRRKIREANK LAEKRKAHDD AVRSEKKAKR TRPV 584
    Length:584
    Mass (Da):67,796
    Last modified:March 1, 2001 - v1
    Checksum:i63D3C352DF5E9893
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF289539 mRNA. Translation: AAG40734.1.
    AK029075 mRNA. Translation: BAC26278.1.
    AK088831 mRNA. Translation: BAC40599.1.
    BC004844 mRNA. Translation: AAH04844.1.
    BC011142 mRNA. Translation: AAH11142.1.
    CCDSiCCDS24372.1.
    RefSeqiNP_075027.1. NM_022889.3.
    UniGeneiMm.28659.

    Genome annotation databases

    EnsembliENSMUST00000020705; ENSMUSP00000020705; ENSMUSG00000020430.
    GeneIDi64934.
    KEGGimmu:64934.
    UCSCiuc007hua.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF289539 mRNA. Translation: AAG40734.1 .
    AK029075 mRNA. Translation: BAC26278.1 .
    AK088831 mRNA. Translation: BAC40599.1 .
    BC004844 mRNA. Translation: AAH04844.1 .
    BC011142 mRNA. Translation: AAH11142.1 .
    CCDSi CCDS24372.1.
    RefSeqi NP_075027.1. NM_022889.3.
    UniGenei Mm.28659.

    3D structure databases

    ProteinModelPortali Q9EQ61.
    SMRi Q9EQ61. Positions 321-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211118. 1 interaction.
    IntActi Q9EQ61. 1 interaction.
    MINTi MINT-4107198.

    PTM databases

    PhosphoSitei Q9EQ61.

    Proteomic databases

    PaxDbi Q9EQ61.
    PRIDEi Q9EQ61.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020705 ; ENSMUSP00000020705 ; ENSMUSG00000020430 .
    GeneIDi 64934.
    KEGGi mmu:64934.
    UCSCi uc007hua.1. mouse.

    Organism-specific databases

    CTDi 23481.
    MGIi MGI:1890613. Pes1.

    Phylogenomic databases

    eggNOGi COG5163.
    GeneTreei ENSGT00390000002626.
    HOGENOMi HOG000006022.
    HOVERGENi HBG023409.
    KOi K14843.

    Miscellaneous databases

    NextBioi 320241.
    PROi Q9EQ61.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9EQ61.
    Bgeei Q9EQ61.
    Genevestigatori Q9EQ61.

    Family and domain databases

    Gene3Di 3.40.50.10190. 1 hit.
    HAMAPi MF_03028. Pescadillo.
    InterProi IPR001357. BRCT_dom.
    IPR010613. PES.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF06732. Pescadillo_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52113. SSF52113. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The murine Pes1 gene encodes a nuclear protein containing a BRCT domain."
      Haque J., Boger S., Li J., Duncan S.A.
      Genomics 70:201-210(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Skin and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon and Mammary gland.
    4. Cited for: INDUCTION, DEVELOPMENTAL STAGE.
    5. "Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation."
      Lerch-Gaggl A.F., Haque J., Li J., Ning G., Traktman P., Duncan S.A.
      J. Biol. Chem. 277:45347-45355(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE.
    6. "Physical and functional interaction between Pes1 and Bop1 in mammalian ribosome biogenesis."
      Lapik Y.R., Fernandes C.J., Lau L.F., Pestov D.G.
      Mol. Cell 15:17-29(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BOP1, SUBCELLULAR LOCATION.
    7. "Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells."
      Prisco M., Maiorana A., Guerzoni C., Calin G., Calabretta B., Voit R., Grummt I., Baserga R.
      Mol. Cell. Biol. 24:5421-5433(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IRS1 AND UBTF, SUBCELLULAR LOCATION.
    8. "Light chain 1 of microtubule-associated protein 1B can negatively regulate the action of Pes1."
      Lerch-Gaggl A.F., Sun K., Duncan S.A.
      J. Biol. Chem. 282:11308-11316(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MAP1B, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPESC_MOUSE
    AccessioniPrimary (citable) accession number: Q9EQ61
    Secondary accession number(s): Q542F0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3