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Protein

Voltage-dependent T-type calcium channel subunit alpha-1H

Gene

Cacna1h

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi140 – 1401ZincBy similarity
Metal bindingi189 – 1891ZincBy similarity
Metal bindingi191 – 1911ZincBy similarity
Sitei378 – 3781Calcium ion selectivity and permeabilityBy similarity
Sitei971 – 9711Calcium ion selectivity and permeabilityBy similarity
Sitei1515 – 15151Calcium ion selectivity and permeabilityBy similarity
Sitei1813 – 18131Calcium ion selectivity and permeabilityBy similarity

GO - Molecular functioni

  • low voltage-gated calcium channel activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • aldosterone biosynthetic process Source: UniProtKB
  • calcium ion import Source: RGD
  • calcium ion transport Source: RGD
  • cortisol biosynthetic process Source: UniProtKB
  • membrane depolarization during action potential Source: GO_Central
  • positive regulation of calcium ion-dependent exocytosis Source: GO_Central
  • sensory perception of pain Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent T-type calcium channel subunit alpha-1H
Alternative name(s):
Voltage-gated calcium channel subunit alpha Cav3.2
Gene namesi
Name:Cacna1h
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68943. Cacna1h.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 100100CytoplasmicSequence analysisAdd
BLAST
Transmembranei101 – 11919Helical; Name=S1 of repeat ISequence analysisAdd
BLAST
Topological domaini120 – 14122ExtracellularSequence analysisAdd
BLAST
Transmembranei142 – 16019Helical; Name=S2 of repeat ISequence analysisAdd
BLAST
Topological domaini161 – 1699CytoplasmicSequence analysis
Transmembranei170 – 18415Helical; Name=S3 of repeat ISequence analysisAdd
BLAST
Topological domaini185 – 1939ExtracellularSequence analysis
Transmembranei194 – 21219Helical; Name=S4 of repeat ISequence analysisAdd
BLAST
Topological domaini213 – 23220CytoplasmicSequence analysisAdd
BLAST
Transmembranei233 – 25321Helical; Name=S5 of repeat ISequence analysisAdd
BLAST
Topological domaini254 – 394141ExtracellularSequence analysisAdd
BLAST
Transmembranei395 – 41925Helical; Name=S6 of repeat ISequence analysisAdd
BLAST
Topological domaini420 – 790371CytoplasmicSequence analysisAdd
BLAST
Transmembranei791 – 81121Helical; Name=S1 of repeat IISequence analysisAdd
BLAST
Topological domaini812 – 82413ExtracellularSequence analysisAdd
BLAST
Transmembranei825 – 84622Helical; Name=S2 of repeat IISequence analysisAdd
BLAST
Topological domaini847 – 8526CytoplasmicSequence analysis
Transmembranei853 – 87119Helical; Name=S3 of repeat IISequence analysisAdd
BLAST
Topological domaini872 – 8798ExtracellularSequence analysis
Transmembranei880 – 90324Helical; Name=S4 of repeat IISequence analysisAdd
BLAST
Topological domaini904 – 91411CytoplasmicSequence analysisAdd
BLAST
Transmembranei915 – 93521Helical; Name=S5 of repeat IISequence analysisAdd
BLAST
Topological domaini936 – 98752ExtracellularSequence analysisAdd
BLAST
Transmembranei988 – 101225Helical; Name=S6 of repeat IISequence analysisAdd
BLAST
Topological domaini1013 – 1301289CytoplasmicSequence analysisAdd
BLAST
Transmembranei1302 – 132423Helical; Name=S1 of repeat IIISequence analysisAdd
BLAST
Topological domaini1325 – 134218ExtracellularSequence analysisAdd
BLAST
Transmembranei1343 – 136321Helical; Name=S2 of repeat IIISequence analysisAdd
BLAST
Topological domaini1364 – 137310CytoplasmicSequence analysis
Transmembranei1374 – 139320Helical; Name=S3 of repeat IIISequence analysisAdd
BLAST
Topological domaini1394 – 140714ExtracellularSequence analysisAdd
BLAST
Transmembranei1408 – 142922Helical; Name=S4 of repeat IIISequence analysisAdd
BLAST
Topological domaini1430 – 143910CytoplasmicSequence analysis
Transmembranei1440 – 146324Helical; Name=S5 of repeat IIISequence analysisAdd
BLAST
Topological domaini1464 – 154077ExtracellularSequence analysisAdd
BLAST
Transmembranei1541 – 156626Helical; Name=S6 of repeat IIISequence analysisAdd
BLAST
Topological domaini1567 – 162155CytoplasmicSequence analysisAdd
BLAST
Transmembranei1622 – 164221Helical; Name=S1 of repeat IVSequence analysisAdd
BLAST
Topological domaini1643 – 165614ExtracellularSequence analysisAdd
BLAST
Transmembranei1657 – 167822Helical; Name=S2 of repeat IVSequence analysisAdd
BLAST
Topological domaini1679 – 16857CytoplasmicSequence analysis
Transmembranei1686 – 170419Helical; Name=S3 of repeat IVSequence analysisAdd
BLAST
Topological domaini1705 – 171814ExtracellularSequence analysisAdd
BLAST
Transmembranei1719 – 174224Helical; Name=S4 of repeat IVSequence analysisAdd
BLAST
Topological domaini1743 – 175614CytoplasmicSequence analysisAdd
BLAST
Transmembranei1757 – 177721Helical; Name=S5 of repeat IVSequence analysisAdd
BLAST
Topological domaini1778 – 184063ExtracellularSequence analysisAdd
BLAST
Transmembranei1841 – 186828Helical; Name=S6 of repeat IVSequence analysisAdd
BLAST
Topological domaini1869 – 2359491CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: RGD
  • dendrite Source: RGD
  • nucleus Source: RGD
  • perikaryon Source: RGD
  • plasma membrane Source: GO_Central
  • voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

GuidetoPHARMACOLOGYi536.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23592359Voltage-dependent T-type calcium channel subunit alpha-1HPRO_0000053956Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence analysis
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence analysis
Glycosylationi1477 – 14771N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9EQ60.
PRIDEiQ9EQ60.

PTM databases

PhosphoSiteiQ9EQ60.

Expressioni

Tissue specificityi

Expressed in brain.

Interactioni

Protein-protein interaction databases

MINTiMINT-4998177.
STRINGi10116.ENSRNOP00000042239.

Structurei

3D structure databases

ProteinModelPortaliQ9EQ60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati87 – 422336IAdd
BLAST
Repeati776 – 1015240IIAdd
BLAST
Repeati1292 – 1569278IIIAdd
BLAST
Repeati1607 – 1868262IVAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi521 – 53111Poly-HisAdd
BLAST
Compositional biasi1594 – 15974Poly-Arg

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2302. Eukaryota.
ENOG410XNP6. LUCA.
HOGENOMiHOG000007742.
HOVERGENiHBG050764.
InParanoidiQ9EQ60.
KOiK04855.
PhylomeDBiQ9EQ60.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR005445. VDCC_T_a1.
IPR030160. VDCC_T_a1H.
[Graphical view]
PANTHERiPTHR10037:SF192. PTHR10037:SF192. 4 hits.
PfamiPF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR01629. TVDCCALPHA1.

Sequencei

Sequence statusi: Complete.

Q9EQ60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEGTLAADE VRVPLGASPP APAAPVRASP ASPGAPGREE QGGSGSGVLA
60 70 80 90 100
PESPGTECGA DLGADEEQPV PYPALAATVF FCLGQTTRPR SWCLRLVCNP
110 120 130 140 150
WFEHISMLVI MLNCVTLGMF RPCEDVECRS ERCSILEAFD DFIFAFFAVE
160 170 180 190 200
MVIKMVALGL FGQKCYLGDT WNRLDFFIVM AGMMEYSLDG HNVSLSAIRT
210 220 230 240 250
VRVLRPLRAI NRVPSMRILV TLLLDTLPML GNVLLLCFFV FFIFGIVGVQ
260 270 280 290 300
LWAGLLRNRC FLDSAFVRNN NLTFLRPYYQ TEEGEENPFI CSSRRDNGMQ
310 320 330 340 350
KCSHIPSRRE LRVQCTLGWE AYGQPQAEDG GAGRNACINW NQYYNVCRSG
360 370 380 390 400
EFNPHNGAIN FDNIGYAWIA IFQVITLEGW VDIMYYVMDA HSFYNFIYFI
410 420 430 440 450
LLIIMGSFFM INLCLVVIAT QFSETKQREN QLMREQRARY LSNDSTLASF
460 470 480 490 500
SEPGSCYEEL LKYVGHIFRK VKRRSLRLYA RWQSRWRKKV DPSSTVHGQG
510 520 530 540 550
PGRRPRRAGR RTASVHHLVY HHHHHHHHHY HFSHGGPRRP SPEPGAGDNR
560 570 580 590 600
LVRACAPPSP PSPGHGPPDS ESVHSIYHAD CHVEGPQERA RVAHSIATAA
610 620 630 640 650
SLKLASGLGT MNYPTILPSG TVNSKGGTSS RPKGLRGAGA PGAAVHSPLS
660 670 680 690 700
LGSPRPYEKI QDVVGEQGLG RASSHLSGLS VPCPLPSPQA GTLTCELKSC
710 720 730 740 750
PYCASALEDP EFEFSGSESG DSDAHGVYEF TQDVRHGDCR DPVQQPHEVG
760 770 780 790 800
TPGHSNERRR TPLRKASQPG GIGHLWASFS GKLRRIVDSK YFNRGIMAAI
810 820 830 840 850
LVNTLSMGVE YHEQPEELTN ALEISNIVFT SMFALEMLLK LLACGPLGYI
860 870 880 890 900
RNPYNIFDGI VVVISVWEIV GQANGGLSVL RTFRLLRVLK LVRFLPALRR
910 920 930 940 950
QLVVLMRTMD NVATFCMLLM LFIFIFSILG MHLFGCKFSL KTDSGDTVPD
960 970 980 990 1000
RKNFDSLLWA IVTVFQILTQ EDWNVVLYNG MASTSSWAAL YFVALMTFGN
1010 1020 1030 1040 1050
YVLFNLLVAI LVEGFQAEGD ATRSDTDEDK TSTQLEGDFD KLRDLRATEM
1060 1070 1080 1090 1100
KMYSLAVTPN GHLEGRGSLP PPLITHTAAT PMPTPKSSPN LDVAHALLDS
1110 1120 1130 1140 1150
RRSSSGSVDP QLGDQKSLAS LRSSPCTPWG PNSAGSSRRS SWNSLGRAPS
1160 1170 1180 1190 1200
LKRRNQCGER ESLLSGEGKG STDDEAEDSR PSTGTHPGAS PGPRATPLRR
1210 1220 1230 1240 1250
AESLDHRSTL DLCPPRPAAL LPTKFHDCNG QMVALPSEFF LRIDSHKEDA
1260 1270 1280 1290 1300
AEFDDDIEDS CCFRLHKVLE PYAPQWCRSR ESWALYLFPP QNRLRVSCQK
1310 1320 1330 1340 1350
VIAHKMFDHV VLVFIFLNCI TIALERPDID PGSTERAFLS VSNYIFTAIF
1360 1370 1380 1390 1400
VVEMMVKVVA LGLLWGEHAY LQSSWNVLDG LLVLVSLVDI IVAMASAGGA
1410 1420 1430 1440 1450
KILGVLRVLR LLRTLRPLRV ISRAPGLKLV VETLISSLRP IGNIVLICCA
1460 1470 1480 1490 1500
FFIIFGILGV QLFKGKFYYC EGTDTRNITT KAECHAAHYR WVRRKYNFDN
1510 1520 1530 1540 1550
LGQALMSLFV LSSKDGWVNI MYDGLDAVGI DQQPVQNHNP WMLLYFISFL
1560 1570 1580 1590 1600
LIVSFFVLNM FVGVVVENFH KCRQHQEAEE ARRREEKRLR RLERRRRKAQ
1610 1620 1630 1640 1650
RRPYYADYSH TRRSIHSLCT SHYLDLFITF IICLNVITMS MEHYNQPKSL
1660 1670 1680 1690 1700
DEALKYCNYV FTIVFVFEAA LKLVAFGFRR FFKDRWNQLD LAIVLLSIMG
1710 1720 1730 1740 1750
IALEEIEMNA ALPINPTIIR IMRVLRIARV LKLLKMATGM RALLDTVVQA
1760 1770 1780 1790 1800
LPQVGNLGLL FMLLFFIYAA LGVELFGRLE CSEDNPCEGL SRHATFTNFG
1810 1820 1830 1840 1850
MAFLTLFRVS TGDNWNGIMK DTLRECTRED KHCLSYLPAL SPVYFVTFML
1860 1870 1880 1890 1900
VAQFVLVNVV VAVLMKHLEE SNKEAREDAE MDAEIELEMA QGSTAQPPPT
1910 1920 1930 1940 1950
AQESQGTQPD TPNLLVVRKV SVSRMLSLPN DSYMFRPVAP AAAPHSHPLQ
1960 1970 1980 1990 2000
EVEMETYTGP VTSAHSPPLE PRASFQVPSA ASSPARVSDP LCALSPRGTP
2010 2020 2030 2040 2050
RSLSLSRILC RQEAMHSESL EGKVDDVGGD SIPDYTEPAE NMSTSQASTG
2060 2070 2080 2090 2100
APRSPPCSPR PASVRTRKHT FGQRCISSRP PTLGGDEAEA ADPADEEVSH
2110 2120 2130 2140 2150
ITSSAHPWPA TEPHSPEASP TASPVKGTMG SGRDPRRFCS VDAQSFLDKP
2160 2170 2180 2190 2200
GRPDAQRWSS VELDNGESHL ESGEVRGRAS ELEPALGSRR KKKMSPPCIS
2210 2220 2230 2240 2250
IEPPTKDEGS SRPPAAEGGN TTLRRRTPSC EAALHRDCPE PTEGPGTGGD
2260 2270 2280 2290 2300
PVAKGERWGQ ASCRAEHLTV PNFAFEPLDM GGPGGDCFLD SDQSVTPEPR
2310 2320 2330 2340 2350
VSSLGAIVPL ILETELSMPS GDCPEKEQGL YLTVPQTPLK KPGSTPATPA

PDDSGDEPV
Length:2,359
Mass (Da):261,113
Last modified:December 20, 2005 - v2
Checksum:i6E708FA1D8E3765E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290213 mRNA. Translation: AAG35187.2.
RefSeqiNP_722521.1. NM_153814.2.
UniGeneiRn.49178.

Genome annotation databases

GeneIDi114862.
KEGGirno:114862.
UCSCiRGD:68943. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290213 mRNA. Translation: AAG35187.2.
RefSeqiNP_722521.1. NM_153814.2.
UniGeneiRn.49178.

3D structure databases

ProteinModelPortaliQ9EQ60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4998177.
STRINGi10116.ENSRNOP00000042239.

Chemistry

GuidetoPHARMACOLOGYi536.

PTM databases

PhosphoSiteiQ9EQ60.

Proteomic databases

PaxDbiQ9EQ60.
PRIDEiQ9EQ60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114862.
KEGGirno:114862.
UCSCiRGD:68943. rat.

Organism-specific databases

CTDi8912.
RGDi68943. Cacna1h.

Phylogenomic databases

eggNOGiKOG2302. Eukaryota.
ENOG410XNP6. LUCA.
HOGENOMiHOG000007742.
HOVERGENiHBG050764.
InParanoidiQ9EQ60.
KOiK04855.
PhylomeDBiQ9EQ60.

Miscellaneous databases

PROiQ9EQ60.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR005445. VDCC_T_a1.
IPR030160. VDCC_T_a1H.
[Graphical view]
PANTHERiPTHR10037:SF192. PTHR10037:SF192. 4 hits.
PfamiPF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR01629. TVDCCALPHA1.
ProtoNetiSearch...

Entry informationi

Entry nameiCAC1H_RAT
AccessioniPrimary (citable) accession number: Q9EQ60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.