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Protein

DNA polymerase delta subunit 3

Gene

Pold3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for optimal DNA polymerase delta activity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiREACT_276436. Processive synthesis on the lagging strand.
REACT_278371. Processive synthesis on the C-strand of the telomere.
REACT_278538. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_283766. Polymerase switching.
REACT_290560. Leading Strand Synthesis.
REACT_303193. Removal of the Flap Intermediate.
REACT_304724. Telomere C-strand (Lagging Strand) Synthesis.
REACT_320650. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
REACT_329955. Removal of the Flap Intermediate from the C-strand.
REACT_330619. Polymerase switching on the C-strand of the telomere.
REACT_331202. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
REACT_342741. Repair synthesis of patch ~27-30 bases long by DNA polymerase.
REACT_358684. Recognition of DNA damage by PCNA-containing replication complex.
REACT_361486. PCNA-Dependent Long Patch Base Excision Repair.
REACT_362050. Termination of translesion DNA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase delta subunit 3
Alternative name(s):
DNA polymerase delta subunit p66
Gene namesi
Name:Pold3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1915217. Pold3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 462461DNA polymerase delta subunit 3PRO_0000186048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei306 – 3061PhosphoserineBy similarity
Modified residuei403 – 4031PhosphoserineBy similarity
Modified residuei405 – 4051PhosphoserineBy similarity
Modified residuei407 – 4071PhosphothreonineBy similarity
Modified residuei409 – 4091PhosphoserineBy similarity
Modified residuei454 – 4541PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9EQ28.
PaxDbiQ9EQ28.
PRIDEiQ9EQ28.

PTM databases

PhosphoSiteiQ9EQ28.

Expressioni

Gene expression databases

BgeeiQ9EQ28.
ExpressionAtlasiQ9EQ28. baseline and differential.

Interactioni

Subunit structurei

Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. Interacts with POLD2. Interacts with PCNA (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9EQ28. 1 interaction.
STRINGi10090.ENSMUSP00000032969.

Structurei

3D structure databases

ProteinModelPortaliQ9EQ28.
SMRiQ9EQ28. Positions 2-144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG83700.
HOGENOMiHOG000008055.
HOVERGENiHBG051397.
InParanoidiQ9EQ28.
PhylomeDBiQ9EQ28.

Family and domain databases

InterProiIPR019038. DNA_polymerase_subunit_Cdc27.
[Graphical view]
PfamiPF09507. CDC27. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EQ28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQLYLENI DEFVTDQNKI VTYKWLSYTL GVHVNQAKQM LYEYVERKRK
60 70 80 90 100
ENSGAQLHVT YLVSGSLIQN GHSCHKVAVV REDKLEAVKS KLAVTASIHV
110 120 130 140 150
YSIQKAMLKD SGPLFNTDYD ILKSNLQNCS KFSAIQCAAA VPRAPAESPS
160 170 180 190 200
SRKYEQSNLQ AASEAQASEL TTNGHGPPAS KQASQQPKGI MGMLISKAAT
210 220 230 240 250
KTQDTNKETK PEAREVTSAS SAGGKAPGKG SVMSNFFGKA AMNKLKVNLD
260 270 280 290 300
SEQAVKEEKT VEQPPVSVTE PKLAAPPAQK KSSRKSEPGK VQQKEKSSRG
310 320 330 340 350
KRVDLSDEEA KETEHLKKKR RRIKLPQSDS SEDEVFEDSP EMYEADSPSP
360 370 380 390 400
PPVSPPPDPM PKTEPPPVKR SSGETKRRRK RVLKSKTFVD EEGCIVTEKV
410 420 430 440 450
YESESCTDSE EELKMKPASA HKPPAAAVKR EPREERKGPK KGAAALGKAN
460
RQVSITGFFQ KK
Length:462
Mass (Da):50,836
Last modified:September 26, 2001 - v2
Checksum:iFF3F67E065A08B36
GO

Sequence cautioni

The sequence AAG45967.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294329 mRNA. Translation: AAG45967.1. Different initiation.
RefSeqiXP_006508212.2. XM_006508149.2.
UniGeneiMm.37562.

Genome annotation databases

GeneIDi67967.
UCSCiuc009imk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294329 mRNA. Translation: AAG45967.1. Different initiation.
RefSeqiXP_006508212.2. XM_006508149.2.
UniGeneiMm.37562.

3D structure databases

ProteinModelPortaliQ9EQ28.
SMRiQ9EQ28. Positions 2-144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EQ28. 1 interaction.
STRINGi10090.ENSMUSP00000032969.

PTM databases

PhosphoSiteiQ9EQ28.

Proteomic databases

MaxQBiQ9EQ28.
PaxDbiQ9EQ28.
PRIDEiQ9EQ28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi67967.
UCSCiuc009imk.1. mouse.

Organism-specific databases

MGIiMGI:1915217. Pold3.

Phylogenomic databases

eggNOGiNOG83700.
HOGENOMiHOG000008055.
HOVERGENiHBG051397.
InParanoidiQ9EQ28.
PhylomeDBiQ9EQ28.

Enzyme and pathway databases

ReactomeiREACT_276436. Processive synthesis on the lagging strand.
REACT_278371. Processive synthesis on the C-strand of the telomere.
REACT_278538. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_283766. Polymerase switching.
REACT_290560. Leading Strand Synthesis.
REACT_303193. Removal of the Flap Intermediate.
REACT_304724. Telomere C-strand (Lagging Strand) Synthesis.
REACT_320650. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
REACT_329955. Removal of the Flap Intermediate from the C-strand.
REACT_330619. Polymerase switching on the C-strand of the telomere.
REACT_331202. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
REACT_342741. Repair synthesis of patch ~27-30 bases long by DNA polymerase.
REACT_358684. Recognition of DNA damage by PCNA-containing replication complex.
REACT_361486. PCNA-Dependent Long Patch Base Excision Repair.
REACT_362050. Termination of translesion DNA synthesis.

Miscellaneous databases

PROiQ9EQ28.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EQ28.
ExpressionAtlasiQ9EQ28. baseline and differential.

Family and domain databases

InterProiIPR019038. DNA_polymerase_subunit_Cdc27.
[Graphical view]
PfamiPF09507. CDC27. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of testicular germ cell gene expression by differential display analysis."
    Anway M.D., Li Y., Griswold M.D.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Testis.
  2. "Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts."
    Hughes P., Tratner I., Ducoux M., Piard K., Baldacci G.
    Nucleic Acids Res. 27:2108-2114(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION.

Entry informationi

Entry nameiDPOD3_MOUSE
AccessioniPrimary (citable) accession number: Q9EQ28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: July 22, 2015
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.