Q9EQ09 (OLR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oxidized low-density lipoprotein receptor 1
Short name=Ox-LDL receptor 1

Alternative name(s):
Lectin-like oxidized LDL receptor 1
Short name=LOX-1
Short name=Lectin-like oxLDL receptor 1
Lectin-type oxidized LDL receptor 1

Cleaved into the following chain:

1. Oxidized low-density lipoprotein receptor 1, soluble form

Gene names

Name:	Olr1
Synonyms:	Lox1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria By similarity.
Subunit structure	Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70 By similarity.
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity. Secreted By similarity. Note: A secreted form also exists By similarity.
Domain	The cytoplasmic region is required for subcellular sorting on the cell surface By similarity. The C-type lectin domain mediates the recognition and binding of oxLDL By similarity. The Neck region contains 3 internal repeats that are only found in rodents.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Biological process	Cell adhesion Immunity Inflammatory response
Cellular component	Cell membrane Membrane Secreted
Domain	Coiled coil Repeat Signal-anchor Transmembrane Transmembrane helix
Ligand	Lectin
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell death Inferred from electronic annotation. Source: Compara inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW leukocyte cell-cell adhesion Inferred from electronic annotation. Source: Compara lipoprotein metabolic process Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to plasma membrane Traceable author statement Ref.1. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro low-density lipoprotein receptor activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Oxidized low-density lipoprotein receptor 1		PRO_0000017445
	Chain	? – 363		Oxidized low-density lipoprotein receptor 1, soluble form		PRO_0000017446
Regions
	Topological domain	1 – 31	31	Cytoplasmic Potential
	Transmembrane	32 – 54	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	55 – 363	309	Extracellular Potential
	Repeat	96 – 141	46	1
	Repeat	142 – 187	46	2
	Repeat	188 – 233	46	3
	Domain	242 – 355	114	C-type lectin
	Region	55 – 241	187	Neck
	Coiled coil	57 – 232	176	Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Disulfide bond	235 ↔ 246		By similarity
	Disulfide bond	262 ↔ 354		By similarity
	Disulfide bond	333 ↔ 346		By similarity
Experimental info
	Sequence conflict	93	1	T → A in AAG44998. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9EQ09 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 8F370C86B58F11A8	FASTA	363	41,643
	10 20 30 40 50 60 MTFDDKMKPA NDEPDQKSCG KKPKGLHLLS SPWWFPAAMT LVILCLVLSV TLIVQWTQLR 70 80 90 100 110 120 QVSDLLKQYQ ANLTQQDRIL EGQMLAQQKA ENTSQESKKE LKGKIDTLTQ KLNEKSKEQE 130 140 150 160 170 180 ELLQKNQNLQ EALQRAANSS EESQRELKGK IDTITRKLDE KSKEQEELLQ MIQNLQEALQ 190 200 210 220 230 240 RAANSSEESQ RELKGKIDTL TLKLNEKSKE QEELLQKNQN LQEALQRAAN FSGPCPQDWL 250 260 270 280 290 300 WHKENCYLFH GPFSWEKNRQ TCQSLGGQLL QINGADDLTF ILQAISHTTS PFWIGLHRKK 310 320 330 340 350 360 PGQPWLWENG TPLNFQFFKT RGVSLQLYSS GNCAYLQDGA VFAENCILIA FSICQKKTNH LQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"High affinity binding of oxidized LDL to mouse lectin-like oxidized LDL receptor (LOX-1)." Hoshikawa H., Sawamura T., Kakutani M., Aoyama T., Nakamura T., Masaki T. Biochem. Biophys. Res. Commun. 245:841-846(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mouse LOX-1 is expressed in mast cells after IgE cross-linking." Park S.-H., Ahn H.-J., Cho J.-J. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Oxidised LDL receptor

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF303744 mRNA. Translation: AAG44998.1. AK154687 mRNA. Translation: BAE32764.1.
IPI	IPI00110227.
PIR	JE0111.
RefSeq	NP_619589.2. NM_138648.2.
UniGene	Mm.293626.
3D structure databases
ProteinModelPortal	Q9EQ09.
SMR	Q9EQ09. Positions 216-359.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032265.
Proteomic databases
PaxDb	Q9EQ09.
PRIDE	Q9EQ09.
Protocols and materials databases
DNASU	108078.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032265; ENSMUSP00000032265; ENSMUSG00000030162.
GeneID	108078.
KEGG	mmu:108078.
Organism-specific databases
CTD	4973.
MGI	MGI:1261434. Olr1.
Phylogenomic databases
eggNOG	NOG242244.
GeneTree	ENSGT00700000104266.
HOGENOM	HOG000220927.
HOVERGEN	HBG056863.
InParanoid	Q3U3M1.
KO	K08763.
OMA	SICQKKA.
OrthoDB	EOG4V9TRM.
Gene expression databases
ArrayExpress	Q9EQ09.
Bgee	Q9EQ09.
Genevestigator	Q9EQ09.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR016187. C-type_lectin_fold. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. False negative. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	360012.
SOURCE	Search...

Entry information

Entry name

OLR1_MOUSE

Accession

Primary (citable) accession number: Q9EQ09
Secondary accession number(s): Q3U3M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program