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Protein

P2Y purinoceptor 12

Gene

P2ry12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Required for normal platelet aggregation and blood coagulation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991ADPBy similarity
Binding sitei103 – 1031ADP; via carbonyl oxygenBy similarity
Binding sitei111 – 1111ADPBy similarity
Binding sitei193 – 1931ADPBy similarity
Binding sitei197 – 1971ADPBy similarity
Binding sitei269 – 2691ADPBy similarity
Binding sitei286 – 2861ADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 1654ADPBy similarity
Nucleotide bindingi181 – 1855ADPBy similarity
Nucleotide bindingi262 – 2654ADPBy similarity

GO - Molecular functioni

  • ADP receptor activity Source: RGD
  • G-protein coupled adenosine receptor activity Source: Ensembl

GO - Biological processi

  • adenosine receptor signaling pathway Source: RGD
  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  • calcium ion transmembrane transport Source: RGD
  • cell projection organization Source: RGD
  • cellular response to ATP Source: ParkinsonsUK-UCL
  • glial cell migration Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • negative regulation of cell differentiation Source: RGD
  • negative regulation of norepinephrine secretion Source: RGD
  • platelet aggregation Source: RGD
  • positive regulation of ion transport Source: Ensembl
  • potassium ion transmembrane transport Source: RGD
  • protein kinase B signaling Source: RGD
  • regulation of calcium ion transport Source: RGD
  • regulation of microglial cell migration Source: ParkinsonsUK-UCL
  • substrate-dependent cell migration, cell extension Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiR-RNO-392170. ADP signalling through P2Y purinoceptor 12.
R-RNO-417957. P2Y receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
P2Y purinoceptor 12
Short name:
P2Y12
Alternative name(s):
P2Y12 platelet ADP receptor
Gene namesi
Name:P2ry12
Synonyms:P2y12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621681. P2ry12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333ExtracellularBy similarityAdd
BLAST
Transmembranei34 – 5623Helical; Name=1By similarityAdd
BLAST
Topological domaini57 – 6711CytoplasmicBy similarityAdd
BLAST
Transmembranei68 – 8821Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 10315ExtracellularBy similarityAdd
BLAST
Transmembranei104 – 12421Helical; Name=3By similarityAdd
BLAST
Topological domaini125 – 14824CytoplasmicBy similarityAdd
BLAST
Transmembranei149 – 16820Helical; Name=4By similarityAdd
BLAST
Topological domaini169 – 19123ExtracellularBy similarityAdd
BLAST
Transmembranei192 – 21322Helical; Name=5By similarityAdd
BLAST
Topological domaini214 – 23926CytoplasmicBy similarityAdd
BLAST
Transmembranei240 – 26526Helical; Name=6By similarityAdd
BLAST
Topological domaini266 – 28419ExtracellularBy similarityAdd
BLAST
Transmembranei285 – 30420Helical; Name=7By similarityAdd
BLAST
Topological domaini305 – 34339CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • basal plasma membrane Source: RGD
  • caveola Source: RGD
  • external side of plasma membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • intracellular Source: GOC
  • intrinsic component of membrane Source: UniProtKB
  • plasma membrane Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343P2Y purinoceptor 12PRO_0000070039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence analysis
Glycosylationi12 – 121N-linked (GlcNAc...)Sequence analysis
Disulfide bondi23 ↔ 276PROSITE-ProRule annotation
Modified residuei61 – 611PhosphoserineCombined sources
Modified residuei63 – 631PhosphoserineCombined sources
Disulfide bondi103 ↔ 181PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9EPX4.
PRIDEiQ9EPX4.

PTM databases

iPTMnetiQ9EPX4.
PhosphoSiteiQ9EPX4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013902.
GenevisibleiQ9EPX4. RN.

Interactioni

Protein-protein interaction databases

BioGridi249199. 1 interaction.
STRINGi10116.ENSRNOP00000018600.

Chemistry

BindingDBiQ9EPX4.

Structurei

3D structure databases

ProteinModelPortaliQ9EPX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The transmembrane domain is composed of seven transmembrane helices; most of these are not strictly perpendicular to the plane of the membrane, but are tilted and/or kinked. Agonist binding promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. Antagonists can bind to an overlapping site, but block the inward movement of the transmembrane helices (By similarity).By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFYD. Eukaryota.
ENOG410ZJD1. LUCA.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000273884.
HOVERGENiHBG108228.
InParanoidiQ9EPX4.
KOiK04298.
OMAiIKVFIII.
OrthoDBiEOG091G0B3H.
PhylomeDBiQ9EPX4.
TreeFamiTF330969.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005394. P2Y12_rcpt.
[Graphical view]
PANTHERiPTHR24233:SF0. PTHR24233:SF0. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01569. P2Y12PRNCPTR.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVPGANATS ANTTSIPGTS TLCSRDYKIT QVLFPLLYTV LFFAGLITNS
60 70 80 90 100
LAMRIFFQIR SKSNFIIFLK NTVISDLLMI LTFPFKILSD AKLGAGHLRT
110 120 130 140 150
LVCQVTSVTF YFTMYISISF LGLITIDRYL KTTRPFKTSS PSNLLGAKIL
160 170 180 190 200
SVAIWAFMFL LSLPNMILTN RRPKDKDITK CSFLKSEFGL VWHEIVNYIC
210 220 230 240 250
QVIFWINFLI VIVCYSLITK ELYRSYVRTR GSAKAPKKRV NIKVFIIIAV
260 270 280 290 300
FFICFVPFHF ARIPYTLSQT RAVFDCNAEN TLFYVKESTL WLTSLNACLD
310 320 330 340
PFIYFFLCKS FRNSLMSMLR CSTSGANKKK GQEGGDPSEE TPM
Length:343
Mass (Da):39,047
Last modified:March 1, 2001 - v1
Checksum:i7AE0AFCE66674136
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF313450 mRNA. Translation: AAG48945.1.
RefSeqiNP_073637.1. NM_022800.1.
XP_006232468.1. XM_006232406.2.
XP_006232469.1. XM_006232407.2.
XP_006232470.1. XM_006232408.2.
UniGeneiRn.64592.

Genome annotation databases

EnsembliENSRNOT00000018600; ENSRNOP00000018600; ENSRNOG00000013902.
GeneIDi64803.
KEGGirno:64803.
UCSCiRGD:621681. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF313450 mRNA. Translation: AAG48945.1.
RefSeqiNP_073637.1. NM_022800.1.
XP_006232468.1. XM_006232406.2.
XP_006232469.1. XM_006232407.2.
XP_006232470.1. XM_006232408.2.
UniGeneiRn.64592.

3D structure databases

ProteinModelPortaliQ9EPX4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249199. 1 interaction.
STRINGi10116.ENSRNOP00000018600.

Chemistry

BindingDBiQ9EPX4.
ChEMBLiCHEMBL2188.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ9EPX4.
PhosphoSiteiQ9EPX4.

Proteomic databases

PaxDbiQ9EPX4.
PRIDEiQ9EPX4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018600; ENSRNOP00000018600; ENSRNOG00000013902.
GeneIDi64803.
KEGGirno:64803.
UCSCiRGD:621681. rat.

Organism-specific databases

CTDi64805.
RGDi621681. P2ry12.

Phylogenomic databases

eggNOGiENOG410IFYD. Eukaryota.
ENOG410ZJD1. LUCA.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000273884.
HOVERGENiHBG108228.
InParanoidiQ9EPX4.
KOiK04298.
OMAiIKVFIII.
OrthoDBiEOG091G0B3H.
PhylomeDBiQ9EPX4.
TreeFamiTF330969.

Enzyme and pathway databases

ReactomeiR-RNO-392170. ADP signalling through P2Y purinoceptor 12.
R-RNO-417957. P2Y receptors.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiQ9EPX4.

Gene expression databases

BgeeiENSRNOG00000013902.
GenevisibleiQ9EPX4. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005394. P2Y12_rcpt.
[Graphical view]
PANTHERiPTHR24233:SF0. PTHR24233:SF0. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01569. P2Y12PRNCPTR.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2Y12_RAT
AccessioniPrimary (citable) accession number: Q9EPX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.