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Q9EPW9 (TLR6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toll-like receptor 6
Alternative name(s):
CD_antigen=CD286
Gene names
Name:Tlr6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length795 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Cooperates with TLR2 for the cellular activation. Ref.3

Subunit structure

Binds MYD88 via their respective TIR domains By similarity. Heterodimer with TLR2 via their respective extracellular domains. Ref.3

Subcellular location

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesiclephagosome membrane; Single-pass type I membrane protein.

Tissue specificity

Detected in thymus, spleen, ovary and lung.

Sequence similarities

Belongs to the Toll-like receptor family.

Contains 19 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 TIR domain.

Sequence caution

The sequence AAG38563.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA78632.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentCell membrane
Cytoplasmic vesicle
Membrane
   DomainLeucine-rich repeat
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMyD88-dependent toll-like receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

T-helper 1 type immune response

Inferred by curator PubMed 11686851. Source: UniProtKB

cellular response to diacyl bacterial lipopeptide

Inferred from electronic annotation. Source: Ensembl

defense response to bacterium

Inferred from electronic annotation. Source: Ensembl

detection of diacyl bacterial lipopeptide

Inferred from electronic annotation. Source: InterPro

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

microglial cell activation involved in immune response

Inferred from electronic annotation. Source: Ensembl

nitric oxide metabolic process

Inferred from electronic annotation. Source: Ensembl

pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response

Inferred from electronic annotation. Source: Ensembl

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of JUN kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-12 biosynthetic process

Non-traceable author statement PubMed 11686851. Source: UniProtKB

positive regulation of interleukin-6 biosynthetic process

Inferred from sequence or structural similarity PubMed 12077222. Source: UniProtKB

regulation of cytokine secretion

Inferred from electronic annotation. Source: InterPro

toll-like receptor 6 signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentToll-like receptor 2-Toll-like receptor 6 protein complex

Inferred from direct assay Ref.3. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

phagocytic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiacyl lipopeptide binding

Inferred from direct assay Ref.3. Source: MGI

lipopeptide binding

Inferred from mutant phenotype PubMed 11431423. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction Ref.3. Source: MGI

transmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 795768Toll-like receptor 6
PRO_0000034732

Regions

Topological domain28 – 584557Extracellular Potential
Transmembrane585 – 60521Helical; Potential
Topological domain606 – 795190Cytoplasmic Potential
Repeat54 – 7724LRR 1
Repeat78 – 10124LRR 2
Repeat102 – 12524LRR 3
Repeat126 – 15025LRR 4
Repeat151 – 17525LRR 5
Repeat176 – 19924LRR 6
Repeat200 – 22324LRR 7
Repeat224 – 25027LRR 8
Repeat251 – 27828LRR 9
Repeat279 – 30830LRR 10
Repeat309 – 33729LRR 11
Repeat338 – 36124LRR 12
Repeat362 – 38827LRR 13
Repeat389 – 41426LRR 14
Repeat415 – 43723LRR 15
Repeat438 – 45720LRR 16
Repeat458 – 47821LRR 17
Repeat479 – 50022LRR 18
Repeat501 – 52424LRR 19
Domain525 – 57652LRRCT
Domain640 – 784145TIR

Amino acid modifications

Glycosylation421N-linked (GlcNAc...) Potential
Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation1441N-linked (GlcNAc...) Ref.3
Glycosylation1951N-linked (GlcNAc...) Ref.3
Glycosylation2141N-linked (GlcNAc...) Ref.3
Glycosylation2531N-linked (GlcNAc...) Ref.3
Glycosylation2851N-linked (GlcNAc...)
Glycosylation3591N-linked (GlcNAc...) Ref.3
Glycosylation4011N-linked (GlcNAc...) Ref.3
Glycosylation4341N-linked (GlcNAc...) Ref.3
Disulfide bond117 ↔ 139 Ref.3
Disulfide bond235 ↔ 265 Ref.3
Disulfide bond348 ↔ 373 Ref.3
Disulfide bond424 ↔ 447 Ref.3

Experimental info

Mutagenesis6801P → H: Dominant negative mutant, blocks response to Gram-positive pathogens. Ref.2
Sequence conflict1811Y → H in BAA78632. Ref.1

Secondary structure

...................................................................... 795
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9EPW9 [UniParc].

Last modified January 31, 2002. Version 2.
Checksum: 34D8BD175A26C233

FASTA79591,116
        10         20         30         40         50         60 
MSQDRKPIVG SFHFVCALAL IVGSMTPFSN ELESMVDYSN RNLTHVPKDL PPRTKALSLS 

        70         80         90        100        110        120 
QNSISELRMP DISFLSELRV LRLSHNRIRS LDFHVFLFNQ DLEYLDVSHN RLQNISCCPM 

       130        140        150        160        170        180 
ASLRHLDLSF NDFDVLPVCK EFGNLTKLTF LGLSAAKFRQ LDLLPVAHLH LSCILLDLVS 

       190        200        210        220        230        240 
YHIKGGETES LQIPNTTVLH LVFHPNSLFS VQVNMSVNAL GHLQLSNIKL NDENCQRLMT 

       250        260        270        280        290        300 
FLSELTRGPT LLNVTLQHIE TTWKCSVKLF QFFWPRPVEY LNIYNLTITE RIDREEFTYS 

       310        320        330        340        350        360 
ETALKSLMIE HVKNQVFLFS KEALYSVFAE MNIKMLSISD TPFIHMVCPP SPSSFTFLNF 

       370        380        390        400        410        420 
TQNVFTDSVF QGCSTLKRLQ TLILQRNGLK NFFKVALMTK NMSSLETLDV SLNSLNSHAY 

       430        440        450        460        470        480 
DRTCAWAESI LVLNLSSNML TGSVFRCLPP KVKVLDLHNN RIMSIPKDVT HLQALQELNV 

       490        500        510        520        530        540 
ASNSLTDLPG CGAFSSLSVL VIDHNSVSHP SEDFFQSCQN IRSLTAGNNP FQCTCELRDF 

       550        560        570        580        590        600 
VKNIGWVARE VVEGWPDSYR CDYPESSKGT ALRDFHMSPL SCDTVLLTVT IGATMLVLAV 

       610        620        630        640        650        660 
TGAFLCLYFD LPWYVRMLCQ WTQTRHRARH IPLEELQRNL QFHAFVSYSE HDSAWVKNEL 

       670        680        690        700        710        720 
LPNLEKDDIR VCLHERNFVP GKSIVENIIN FIEKSYKAIF VLSPHFIQSE WCHYELYFAH 

       730        740        750        760        770        780 
HNLFHEGSDN LILILLEPIL QNNIPSRYHK LRALMAQRTY LEWPTEKGKR GLFWANLRAS 

       790 
FIMKLALVNE DDVKT 

« Hide

References

[1]"TLR6: a novel member of an expanding Toll-like receptor family."
Takeuchi O., Kawai T., Sanjo H., Copeland N.G., Gilbert D.J., Jenkins N.A., Takeda K., Akira S.
Gene 231:59-65(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]"The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between Toll-like receptors."
Ozinsky A., Underhill D.M., Fontenot J.D., Hajjar A.M., Smith K.D., Wilson C.B., Schroeder L., Aderem A.
Proc. Natl. Acad. Sci. U.S.A. 97:13766-13771(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF PRO-680.
Strain: BALB/c.
Tissue: Macrophage.
[3]"Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer."
Kang J.Y., Nan X., Jin M.S., Youn S.J., Ryu Y.H., Mah S., Han S.H., Lee H., Paik S.G., Lee J.O.
Immunity 31:873-884(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 1-483 IN COMPLEX WITH TLR2 AND LIPOPEPTIDE, DISULFIDE BONDS, GLYCOSYLATION AT ASN-144; ASN-195; ASN-214; ASN-253; ASN-359; ASN-401 AND ASN-434, FUNCTION, LRR REPEATS, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB020808 mRNA. Translation: BAA78632.1. Different initiation.
AF314636 mRNA. Translation: AAG38563.1. Different initiation.
RefSeqNP_035734.3. NM_011604.3.
XP_006503920.1. XM_006503857.1.
XP_006503921.1. XM_006503858.1.
XP_006503922.1. XM_006503859.1.
XP_006503923.1. XM_006503860.1.
UniGeneMm.42146.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3A79X-ray2.90B1-483[»]
ProteinModelPortalQ9EPW9.
SMRQ9EPW9. Positions 33-785.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9EPW9. 1 interaction.

PTM databases

PhosphoSiteQ9EPW9.

Proteomic databases

PRIDEQ9EPW9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID21899.
KEGGmmu:21899.

Organism-specific databases

CTD10333.
MGIMGI:1341296. Tlr6.

Phylogenomic databases

eggNOGNOG272762.
HOVERGENHBG023180.
InParanoidQ9EPW9.
KOK10169.
PhylomeDBQ9EPW9.

Gene expression databases

ArrayExpressQ9EPW9.
BgeeQ9EPW9.
CleanExMM_TLR6.
GenevestigatorQ9EPW9.

Family and domain databases

Gene3D3.40.50.10140. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027187. TLR1/6.
IPR017241. Toll-like_receptor.
[Graphical view]
PANTHERPTHR24365:SF130. PTHR24365:SF130. 1 hit.
PfamPF00560. LRR_1. 2 hits.
PF12799. LRR_4. 1 hit.
PF01582. TIR. 1 hit.
[Graphical view]
PIRSFPIRSF037595. Toll-like_receptor. 1 hit.
SMARTSM00369. LRR_TYP. 1 hit.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMSSF52200. SSF52200. 1 hit.
PROSITEPS51450. LRR. 10 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9EPW9.
NextBio301440.
PROQ9EPW9.
SOURCESearch...

Entry information

Entry nameTLR6_MOUSE
AccessionPrimary (citable) accession number: Q9EPW9
Secondary accession number(s): Q9WTQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 31, 2002
Last modified: April 16, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot