Q9EPW9 (TLR6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toll-like receptor 6

Alternative name(s):
CD_antigen=CD286

Gene names

Name:

Tlr6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Cooperates with TLR2 for the cellular activation. Ref.4
Subunit structure	Binds MYD88 via their respective TIR domains By similarity. Heterodimer with TLR2 via their respective extracellular domains. Ref.4
Subcellular location	Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle › phagosome membrane; Single-pass type I membrane protein.
Tissue specificity	Detected in thymus, spleen, ovary and lung.
Sequence similarities	Belongs to the Toll-like receptor family. Contains 19 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 TIR domain.
Sequence caution	The sequence AAG38563.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA78632.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Immunity Inflammatory response Innate immunity
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MyD88-dependent toll-like receptor signaling pathway Inferred from electronic annotation. Source: InterPro T-helper 1 type immune response Inferred by curator PubMed 11686851. Source: UniProtKB cellular response to diacyl bacterial lipopeptide Inferred from electronic annotation. Source: Compara detection of diacyl bacterial lipopeptide Inferred from electronic annotation. Source: Compara inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara positive regulation of JUN kinase activity Inferred from electronic annotation. Source: Compara positive regulation of interleukin-12 biosynthetic process Non-traceable author statement PubMed 11686851. Source: UniProtKB positive regulation of interleukin-6 biosynthetic process Inferred from sequence or structural similarity PubMed 12077222. Source: UniProtKB regulation of cytokine secretion Inferred from electronic annotation. Source: InterPro toll-like receptor 6 signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	Toll-like receptor 2-Toll-like receptor 6 protein complex Inferred from direct assay Ref.4. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	diacyl lipopeptide binding Inferred from direct assay Ref.4. Source: MGI transmembrane signaling receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Potential

Chain

28 – 795

768

Toll-like receptor 6

PRO_0000034732

Regions

Topological domain

28 – 584

557

Extracellular Potential

Transmembrane

585 – 605

21

Helical; Potential

Topological domain

606 – 795

190

Cytoplasmic Potential

Repeat

54 – 77

24

LRR 1

Repeat

78 – 101

24

LRR 2

Repeat

102 – 125

24

LRR 3

Repeat

126 – 150

25

LRR 4

Repeat

151 – 175

25

LRR 5

Repeat

176 – 199

24

LRR 6

Repeat

200 – 223

24

LRR 7

Repeat

224 – 250

27

LRR 8

Repeat

251 – 278

28

LRR 9

Repeat

279 – 308

30

LRR 10

Repeat

309 – 337

29

LRR 11

Repeat

338 – 361

24

LRR 12

Repeat

362 – 388

27

LRR 13

Repeat

389 – 414

26

LRR 14

Repeat

415 – 437

23

LRR 15

Repeat

438 – 457

20

LRR 16

Repeat

458 – 478

21

LRR 17

Repeat

479 – 500

22

LRR 18

Repeat

501 – 524

24

LRR 19

Domain

525 – 576

52

LRRCT

Domain

640 – 784

145

TIR

Amino acid modifications

Modified residue

780

1

Phosphoserine Ref.3

Glycosylation

42

1

N-linked (GlcNAc...) Potential

Glycosylation

114

1

N-linked (GlcNAc...) Potential

Glycosylation

144

1

N-linked (GlcNAc...) Ref.4

Glycosylation

195

1

N-linked (GlcNAc...) Ref.4

Glycosylation

214

1

N-linked (GlcNAc...) Ref.4

Glycosylation

253

1

N-linked (GlcNAc...) Ref.4

Glycosylation

285

1

N-linked (GlcNAc...)

Glycosylation

359

1

N-linked (GlcNAc...) Ref.4

Glycosylation

401

1

N-linked (GlcNAc...) Ref.4

Glycosylation

434

1

N-linked (GlcNAc...) Ref.4

Disulfide bond

117 ↔ 139

Ref.4

Disulfide bond

235 ↔ 265

Ref.4

Disulfide bond

348 ↔ 373

Ref.4

Disulfide bond

424 ↔ 447

Ref.4

Experimental info

Mutagenesis

680

1

P → H: Dominant negative mutant, blocks response to Gram-positive pathogens. Ref.2

Sequence conflict

181

1

Y → H in BAA78632. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

795

Helix Strand Turn

Details...

Beta strand

35 – 37

3

Beta strand

56 – 58

3

Helix

69 – 71

3

Turn

72 – 74

3

Beta strand

80 – 82

3

Turn

93 – 98

6

Beta strand

104 – 106

3

Beta strand

124 – 127

4

Helix

140 – 144

5

Beta strand

150 – 154

5

Turn

160 – 163

4

Helix

164 – 166

3

Beta strand

171 – 180

10

Beta strand

185 – 187

3

Beta strand

190 – 193

4

Beta strand

196 – 203

8

Beta strand

205 – 207

3

Beta strand

214 – 229

16

Helix

235 – 246

12

Beta strand

252 – 261

10

Helix

263 – 273

11

Beta strand

276 – 288

13

Beta strand

306 – 314

9

Helix

321 – 329

9

Beta strand

334 – 341

8

Beta strand

357 – 359

3

Turn

367 – 372

6

Beta strand

381 – 383

3

Helix

394 – 397

4

Turn

398 – 401

4

Beta strand

407 – 409

3

Beta strand

432 – 434

3

Helix

442 – 445

4

Beta strand

453 – 456

4

Turn

467 – 470

4

Beta strand

476 – 479

4

Sequences

	Sequence		Length	Mass (Da)
	Q9EPW9 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: 34D8BD175A26C233	FASTA	795	91,116
	10 20 30 40 50 60 MSQDRKPIVG SFHFVCALAL IVGSMTPFSN ELESMVDYSN RNLTHVPKDL PPRTKALSLS 70 80 90 100 110 120 QNSISELRMP DISFLSELRV LRLSHNRIRS LDFHVFLFNQ DLEYLDVSHN RLQNISCCPM 130 140 150 160 170 180 ASLRHLDLSF NDFDVLPVCK EFGNLTKLTF LGLSAAKFRQ LDLLPVAHLH LSCILLDLVS 190 200 210 220 230 240 YHIKGGETES LQIPNTTVLH LVFHPNSLFS VQVNMSVNAL GHLQLSNIKL NDENCQRLMT 250 260 270 280 290 300 FLSELTRGPT LLNVTLQHIE TTWKCSVKLF QFFWPRPVEY LNIYNLTITE RIDREEFTYS 310 320 330 340 350 360 ETALKSLMIE HVKNQVFLFS KEALYSVFAE MNIKMLSISD TPFIHMVCPP SPSSFTFLNF 370 380 390 400 410 420 TQNVFTDSVF QGCSTLKRLQ TLILQRNGLK NFFKVALMTK NMSSLETLDV SLNSLNSHAY 430 440 450 460 470 480 DRTCAWAESI LVLNLSSNML TGSVFRCLPP KVKVLDLHNN RIMSIPKDVT HLQALQELNV 490 500 510 520 530 540 ASNSLTDLPG CGAFSSLSVL VIDHNSVSHP SEDFFQSCQN IRSLTAGNNP FQCTCELRDF 550 560 570 580 590 600 VKNIGWVARE VVEGWPDSYR CDYPESSKGT ALRDFHMSPL SCDTVLLTVT IGATMLVLAV 610 620 630 640 650 660 TGAFLCLYFD LPWYVRMLCQ WTQTRHRARH IPLEELQRNL QFHAFVSYSE HDSAWVKNEL 670 680 690 700 710 720 LPNLEKDDIR VCLHERNFVP GKSIVENIIN FIEKSYKAIF VLSPHFIQSE WCHYELYFAH 730 740 750 760 770 780 HNLFHEGSDN LILILLEPIL QNNIPSRYHK LRALMAQRTY LEWPTEKGKR GLFWANLRAS 790 FIMKLALVNE DDVKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TLR6: a novel member of an expanding Toll-like receptor family." Takeuchi O., Kawai T., Sanjo H., Copeland N.G., Gilbert D.J., Jenkins N.A., Takeda K., Akira S. Gene 231:59-65(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between Toll-like receptors." Ozinsky A., Underhill D.M., Fontenot J.D., Hajjar A.M., Smith K.D., Wilson C.B., Schroeder L., Aderem A. Proc. Natl. Acad. Sci. U.S.A. 97:13766-13771(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF PRO-680. Strain: BALB/c. Tissue: Macrophage.
[3]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-780, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer." Kang J.Y., Nan X., Jin M.S., Youn S.J., Ryu Y.H., Mah S., Han S.H., Lee H., Paik S.G., Lee J.O. Immunity 31:873-884(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 1-483 IN COMPLEX WITH TLR2 AND LIPOPEPTIDE, DISULFIDE BONDS, GLYCOSYLATION AT ASN-144; ASN-195; ASN-214; ASN-253; ASN-359; ASN-401 AND ASN-434, FUNCTION, LRR REPEATS, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB020808 mRNA. Translation: BAA78632.1. Different initiation.
AF314636 mRNA. Translation: AAG38563.1. Different initiation.

IPI

IPI00380390.

RefSeq

NP_035734.3. NM_011604.3.

UniGene

Mm.42146.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3A79	X-ray	2.90	B	1-483	[»]

ProteinModelPortal

Q9EPW9.

SMR

Q9EPW9. Positions 33-785.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9EPW9. 1 interaction.

PTM databases

PhosphoSite

Q9EPW9.

Proteomic databases

PRIDE

Q9EPW9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

21899.

KEGG

mmu:21899.

Organism-specific databases

CTD

10333.

MGI

MGI:1341296. Tlr6.

Phylogenomic databases

eggNOG

NOG272762.

HOVERGEN

HBG023180.

InParanoid

Q9EPW9.

KO

K10169.

OrthoDB

EOG4SBDXJ.

Gene expression databases

ArrayExpress

Q9EPW9.

Bgee

Q9EPW9.

CleanEx

MM_TLR6.

Genevestigator

Q9EPW9.

GermOnline

ENSMUSG00000051498. Mus musculus.

Family and domain databases

InterPro

IPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027187. TLR6.
IPR017241. Toll-like_receptor.
[Graphical view]

PANTHER

PTHR24365:SF130. PTHR24365:SF130. 1 hit.

Pfam

PF00560. LRR_1. 2 hits.
PF01582. TIR. 1 hit.
[Graphical view]

PIRSF

PIRSF037595. Toll-like_receptor. 1 hit.

SMART

SM00369. LRR_TYP. 1 hit.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]

SUPFAM

SSF52200. TIR. 1 hit.

PROSITE

PS51450. LRR. 10 hits.
PS50104. TIR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9EPW9.

NextBio

301440.

SOURCE

Search...

Entry information

Entry name

TLR6_MOUSE

Accession

Primary (citable) accession number: Q9EPW9
Secondary accession number(s): Q9WTQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	January 31, 2002
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program