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Protein

Krueppel-like factor 15

Gene

Klf15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that binds to the GA element of the CLCNKA promoter (By similarity). Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CTGF expression, involved in the control of cardiac fibrosis. Is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A, GATA4 and MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation (By similarity). Involved in podocyte differentiation.By similarity7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri320 – 34425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri350 – 37425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri380 – 40223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: MGI
  4. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. cardiac muscle hypertrophy in response to stress Source: UniProtKB
  2. cellular response to peptide Source: Ensembl
  3. glial cell differentiation Source: MGI
  4. glomerular visceral epithelial cell differentiation Source: UniProtKB
  5. glucose transport Source: MGI
  6. negative regulation of peptidyl-lysine acetylation Source: UniProtKB
  7. positive regulation of transcription, DNA-templated Source: MGI
  8. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  9. regulation of gene expression Source: UniProtKB
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 15
Alternative name(s):
Cardiovascular Krueppel-like factor
Gene namesi
Name:Klf15
Synonyms:Cklf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1929988. Klf15.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

KLF15 null mice are viable, but in response to pressure overload, they develop cardiac hypertrophy and fibrosis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Krueppel-like factor 15PRO_0000047188Add
BLAST

Proteomic databases

PRIDEiQ9EPW2.

PTM databases

PhosphoSiteiQ9EPW2.

Expressioni

Tissue specificityi

Expressed in aortic smooth muscle cells.1 Publication

Inductioni

In the heart, up-regulated by the CLOCK/ARNTL heterodimer. In podocytes, up-regulated by retinoic acid.2 Publications

Gene expression databases

BgeeiQ9EPW2.
CleanExiMM_CKLF.
MM_KLF15.
GenevestigatoriQ9EPW2.

Interactioni

Subunit structurei

Interacts with MYOCD. Interacts with EP300 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9EPW2.
SMRiQ9EPW2. Positions 317-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri320 – 34425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri350 – 37425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri380 – 40223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000294058.
HOVERGENiHBG052266.
InParanoidiQ9EPW2.
KOiK09210.
OMAiTAGVRSY.
OrthoDBiEOG7JMGF2.
PhylomeDBiQ9EPW2.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDHLLPVDE TFSSPKCSVG YLGDRLASRQ PYHMLPSPIS EDDSDVSSPC
60 70 80 90 100
SCASPDSQAF CSCYSAGPGP EAQGSILDFL LSRATLGSGG GSGGIGDSSG
110 120 130 140 150
PVTWGSWRRA SVPVKEEHFC FPEFLSGDTD DVSRPFQPTL EEIEEFLEEN
160 170 180 190 200
MEAEVKEAPE NGSRDLETCS QLSAGSHRSH LHPESAGRER CTPPPGGTSG
210 220 230 240 250
GGAQSAGEGP AHDGPVPVLL QIQPVAVKQE AGTGPASPGQ APESVKVAQL
260 270 280 290 300
LVNIQGQTFA LLPQVVPSSN LNLPSKFVRI APVPIAAKPI GSGSLGPGPA
310 320 330 340 350
GLLVGQKFPK NPAAELLKMH KCTFPGCSKM YTKSSHLKAH LRRHTGEKPF
360 370 380 390 400
ACTWPGCGWR FSRSDELSRH RRSHSGVKPY QCPVCEKKFA RSDHLSKHIK
410
VHRFPRSSRA VRAIN
Length:415
Mass (Da):44,253
Last modified:March 1, 2001 - v1
Checksum:i127A7B80DB3E33CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317225 mRNA. Translation: AAG38597.1.
AK009739 mRNA. Translation: BAB26470.1.
AK142618 mRNA. Translation: BAE25132.1.
BC013486 mRNA. Translation: AAH13486.1.
CCDSiCCDS20360.1.
RefSeqiNP_075673.1. NM_023184.3.
UniGeneiMm.41389.

Genome annotation databases

EnsembliENSMUST00000032174; ENSMUSP00000032174; ENSMUSG00000030087.
ENSMUST00000113530; ENSMUSP00000109158; ENSMUSG00000030087.
GeneIDi66277.
KEGGimmu:66277.
UCSCiuc009cxj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317225 mRNA. Translation: AAG38597.1.
AK009739 mRNA. Translation: BAB26470.1.
AK142618 mRNA. Translation: BAE25132.1.
BC013486 mRNA. Translation: AAH13486.1.
CCDSiCCDS20360.1.
RefSeqiNP_075673.1. NM_023184.3.
UniGeneiMm.41389.

3D structure databases

ProteinModelPortaliQ9EPW2.
SMRiQ9EPW2. Positions 317-402.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9EPW2.

Proteomic databases

PRIDEiQ9EPW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032174; ENSMUSP00000032174; ENSMUSG00000030087.
ENSMUST00000113530; ENSMUSP00000109158; ENSMUSG00000030087.
GeneIDi66277.
KEGGimmu:66277.
UCSCiuc009cxj.1. mouse.

Organism-specific databases

CTDi28999.
MGIiMGI:1929988. Klf15.

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000294058.
HOVERGENiHBG052266.
InParanoidiQ9EPW2.
KOiK09210.
OMAiTAGVRSY.
OrthoDBiEOG7JMGF2.
PhylomeDBiQ9EPW2.
TreeFamiTF350556.

Miscellaneous databases

NextBioi321181.
PROiQ9EPW2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EPW2.
CleanExiMM_CKLF.
MM_KLF15.
GenevestigatoriQ9EPW2.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Kruppel-like factor KLF15 regulates the insulin-sensitive glucose transporter GLUT4."
    Gray S.J., Feinberg M.W., Hull S., Kuo C.T., Watanabe M., Sen-Banerjee S., DePina A., Haspel R., Jain M.K.
    J. Biol. Chem. 277:34322-34328(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland and Tongue.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. Cited for: FUNCTION AS INHIBITOR OF CARDIAC HYPERTROPHY, FUNCTION AS REPRESSOR OF GATA4 AND MEF2A, DISRUPTION PHENOTYPE.
  5. "The Kruppel-like factor KLF15 inhibits connective tissue growth factor (CTGF) expression in cardiac fibroblasts."
    Wang B., Haldar S.M., Lu Y., Ibrahim O.A., Fisch S., Gray S., Leask A., Jain M.K.
    J. Mol. Cell. Cardiol. 45:193-197(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS INHIBITOR OF CARDIAC FIBROSIS AND CTGF EXPRESSION, DISRUPTION PHENOTYPE.
  6. Cited for: FUNCTION AS NEGATIVE REGULATOR OF MYOCD, INTERACTION WITH MYOCD.
  7. Cited for: FUNCTION AS NEGATIVE REGULATOR OF TP53 ACETYLATION.
  8. Cited for: FUNCTION IN PODOCYTE DIFFERENTIATION, INDUCTION.
  9. Cited for: FUNCTION IN KCNIP2 CIRCADIAN EXPRESSION, INDUCTION, BINDING TO THE KCNIP2 PROMOTER.
  10. Cited for: FUNCTION AS INHIBITOR OF VASCULAR INFLAMMATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiKLF15_MOUSE
AccessioniPrimary (citable) accession number: Q9EPW2
Secondary accession number(s): Q3UQB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: March 1, 2001
Last modified: February 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.