Reviewed, UniProtKB/Swiss-Prot Q9EPW0 (INP4A_MOUSE)

Names and origin

Protein names

Recommended name:
    Type I inositol-3,4-bisphosphate 4-phosphatase
    EC=3.1.3.66
Alternative name(s):
    Inositol polyphosphate 4-phosphatase type I

Gene names

Name:

Inpp4a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	939 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate. Involved in the regulation of megakaryocyte and fibroblast proliferation. Regulates cell growth downstream of transcription factor GATA-1. Ref.1
Catalytic activity	1-phosphatidyl-myo-inositol 3,4-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate.
Pathway	Signal transduction; phosphatidylinositol signaling pathway.
Tissue specificity	Spleen, skeletal muscle, lung and uterus. Ref.3
Sequence similarities	Belongs to the inositol-3,4-bisphosphate 4-phosphatase family. Contains 1 C2 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	polyphosphate catabolic process Ref.1 Traceable author statement. Source: MGI
Molecular function	phosphatidyl-inositol-4,5-bisphosphate 4-phosphatase activity Inferred from electronic annotation. Source: EC phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity Ref.1 Traceable author statement. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9EPW0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9EPW0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-265: Missing.
     266-272: LLEEDAA → MCVCFAC
     575-585: Missing.
     897-939: EGCRRENTMK...PEGTYGKVET → IGTREVVTQK...AYLVTKLRCK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 939	939	Type I inositol-3,4-bisphosphate 4-phosphatase		PRO_0000190233
Regions
	Domain	46 – 137	92	C2
Amino acid modifications
	Modified residue	355	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	1 – 265	265	Missing in isoform 2.		VSP_015243
	Alternative sequence	266 – 272	7	LLEEDAA → MCVCFAC in isoform 2.		VSP_015244
	Alternative sequence	575 – 585	11	Missing in isoform 2.		VSP_015245
	Alternative sequence	897 – 939	43	EGCRR…GKVET → IGTREVVTQKNLSGLVPIRD LRLDPSLLCSIPLLALSPNL LIVWLFLSIAYLVTKLRCK in isoform 2.		VSP_015246
Experimental info
	Sequence conflict	529	1	Q → E in BAC29220. Ref.2
	Sequence conflict	553	1	D → E in BAC29220. Ref.2
	Sequence conflict	717	1	S → N in BAC29220. Ref.2
	Sequence conflict	741	1	V → F in BAC29220. Ref.2
	Sequence conflict	744	1	F → L in BAC29220. Ref.2
	Sequence conflict	783	1	G → V in BAC29220. Ref.2
	Sequence conflict	786	1	G → V in BAC29220. Ref.2
	Sequence conflict	792	1	V → L in BAC29220. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FBF3750836FA8962	FASTA	939	105,540
	10 20 30 40 50 60 MTAREHSPRH GARARAMQRA STIDVAADMV GLSLAGNIQD PDEPILEFSL ACSELHTPSL 70 80 90 100 110 120 DRKPNSFVAV SVTTPPQAFW TKHAQTEIIE GTNNPIFLSS IAFFQDSLIN QMTQIKLSVY 130 140 150 160 170 180 DVKDRSQGTM YLLGSGTFVV KDLLQDRHHR LHLTLRSAES DRVGNITVIG WQMEEKSDQQ 190 200 210 220 230 240 PPVTRFLDTV NGRMVLPVDE SLTEALGIRS KYAFLRKDSL LKAVFGGAIC RMYRFPTTDG 250 260 270 280 290 300 NHLRILEQMA ESVLSLHVPR QFVKLLLEED AARVCELEEL GELSPCWESL RRQIVTQYQT 310 320 330 340 350 360 IILTYQENLT DLHQYKGPSF KASSLKADKK LEFVPTNLHI QRMRVQDDGG SDQNYDVVTI 370 380 390 400 410 420 GAPAAHCQGF KSGGLRKKLH KFEEAKKHSF EECCTSSSGQ SIIYIPQDIT RAKEIIAQIN 430 440 450 460 470 480 TLKTQVSYYA ERLSRAAKDR SATGLERTLA ILADKTRQLV TVCDCKLLAN SIHGLNAARP 490 500 510 520 530 540 DYIASKASPT STEEEQVMLR NDQDTLMARW AGRSSRSSLQ VDWHEEEWQK VWLNVDKSLE 550 560 570 580 590 600 CIIQRVDKLL QKDRLHGEGC EDAFPCSSTC SSKKDCSPPP EESSPGEWSE ALYPLLTTLT 610 620 630 640 650 660 DCVAMMSDKA KAAMVFLLMQ DSAPTIASYL SLQYRRDVVF CQTLTALICG FIIKLRNCLH 670 680 690 700 710 720 DGGFLRQLYT IGLLAQFESL LSTYGEELAM LEDMSLGIMD LRNVTFKVTQ ATSNASSDML 730 740 750 760 770 780 PVITGNRDGF NVRIPLPGPL VDSFPREIQS GMLLRVQPVL FNVGINEQQT LAERFGDTSL 790 800 810 820 830 840 QEGINGESLV RVNSYFEQFK EVLPEDCLPR SRSQTCLPEL LRFLGQNVHA RKNKNVDILW 850 860 870 880 890 900 QAAEVCRRLN GVRFTSCKSA KDRTAMSVTL EQCLILQHEH GMAPQVFTQA LECMRSEGCR 910 920 930 RENTMKNVGS RKYAFNSLQL KAFPKHYRPP EGTYGKVET
	Isoform 2. Checksum: AC4B87C45EB19C78	FASTA	679	76,356
	10 20 30 40 50 60 MCVCFACRVC ELEELGELSP CWESLRRQIV TQYQTIILTY QENLTDLHQY KGPSFKASSL 70 80 90 100 110 120 KADKKLEFVP TNLHIQRMRV QDDGGSDQNY DVVTIGAPAA HCQGFKSGGL RKKLHKFEEA 130 140 150 160 170 180 KKHSFEECCT SSSGQSIIYI PQDITRAKEI IAQINTLKTQ VSYYAERLSR AAKDRSATGL 190 200 210 220 230 240 ERTLAILADK TRQLVTVCDC KLLANSIHGL NAARPDYIAS KASPTSTEEE QVMLRNDQDT 250 260 270 280 290 300 LMARWAGRSS RSSLQVDWHE EEWQKVWLNV DKSLECIIQR VDKLLQKDRL HGEGCEDAFP 310 320 330 340 350 360 CSSTCSSKKG EWSEALYPLL TTLTDCVAMM SDKAKAAMVF LLMQDSAPTI ASYLSLQYRR 370 380 390 400 410 420 DVVFCQTLTA LICGFIIKLR NCLHDGGFLR QLYTIGLLAQ FESLLSTYGE ELAMLEDMSL 430 440 450 460 470 480 GIMDLRNVTF KVTQATSNAS SDMLPVITGN RDGFNVRIPL PGPLVDSFPR EIQSGMLLRV 490 500 510 520 530 540 QPVLFNVGIN EQQTLAERFG DTSLQEGING ESLVRVNSYF EQFKEVLPED CLPRSRSQTC 550 560 570 580 590 600 LPELLRFLGQ NVHARKNKNV DILWQAAEVC RRLNGVRFTS CKSAKDRTAM SVTLEQCLIL 610 620 630 640 650 660 QHEHGMAPQV FTQALECMRS IGTREVVTQK NLSGLVPIRD LRLDPSLLCS IPLLALSPNL 670 LIVWLFLSIA YLVTKLRCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inositol polyphosphate 4-phosphatase type I regulates cell growth downstream of transcription factor GATA-1." Vyas P., Norris F.A., Joseph R., Majerus P.W., Orkin S.H. Proc. Natl. Acad. Sci. U.S.A. 97:13696-13701(2000) [PubMed: 11087841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Cerebellum.
[3]	"Identification of a novel spliceoform of inositol polyphosphate 4-phosphatase type Ialpha expressed in human platelets: structure of human inositol polyphosphate 4-phosphatase type I gene." Shearn C.T., Walker J., Norris F.A. Biochem. Biophys. Res. Commun. 286:119-125(2001) [PubMed: 11485317] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF317838 mRNA. Translation: AAG40778.1. AK035867 mRNA. Translation: BAC29220.1.
IPI	IPI00110426. IPI00785480.
UniGene	Mm.425030 Mm.5294
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q9EPW0.
Proteomic databases
PRIDE	Q9EPW0.
Genome annotation databases
Ensembl	ENSMUSG00000026113. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1931123. Inpp4a.
Phylogenomic databases
HOGENOM	Q9EPW0.
HOVERGEN	Q9EPW0.
Enzyme and pathway databases
BRENDA	3.1.3.66. 244.
Gene expression databases
ArrayExpress	Q9EPW0.
Bgee	Q9EPW0.
CleanEx	MM_INPP4A.
GermOnline	ENSMUSG00000026113. Mus musculus.
Family and domain databases
PROSITE	PS50004. C2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

INP4A_MOUSE

Accession

Primary (citable) accession number: Q9EPW0
Secondary accession number(s): Q8CBJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2001
Last modified:	March 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)