Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger protein 354C

Gene

Znf354c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a transcription repressor. Suppresses osteogenic effects of RUNX2. Binds to 5'-CCACA-3' core sequence. May be involved in osteoblastic differentiation.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri218 – 24023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri246 – 26823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri274 – 29623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri302 – 32423C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri330 – 35223C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri358 – 38023C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri386 – 40823C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri414 – 43623C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri442 – 46423C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri470 – 49223C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri498 – 52023C2H2-type 11PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 354C
Alternative name(s):
Protein AJ18
Gene namesi
Name:Znf354c
Synonyms:Zfp354c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69405. Zfp354c.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Zinc finger protein 354CPRO_0000280410Add
BLAST

Proteomic databases

PaxDbiQ9EPU7.

Expressioni

Tissue specificityi

Expressed in brain. Very low expression in adult bone tissues. Overexpression suppresses alkaline phosphatase induction by BMP7.1 Publication

Developmental stagei

Expressed during osteoblast differentiation and bone development. Detected in embryonic bone formation in calvariae and tibiae. Very low expression in neonate bone tissues. Expressed in kidney and brain.1 Publication

Interactioni

Subunit structurei

Interacts with RUNX2. Binds consensus element OSE2.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000248.

Structurei

3D structure databases

ProteinModelPortaliQ9EPU7.
SMRiQ9EPU7. Positions 227-380, 439-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 8471KRABPROSITE-ProRule annotationAdd
BLAST

Domaini

KRAB domain is not required for nuclear targeting or for DNA binding.
Zinc finger region is involved in nuclear targeting and DNA-binding.

Sequence similaritiesi

Contains 11 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri218 – 24023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri246 – 26823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri274 – 29623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri302 – 32423C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri330 – 35223C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri358 – 38023C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri386 – 40823C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri414 – 43623C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri442 – 46423C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri470 – 49223C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri498 – 52023C2H2-type 11PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ9EPU7.
KOiK09228.
PhylomeDBiQ9EPU7.

Family and domain databases

Gene3Di3.30.160.60. 11 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 2 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 11 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 11 hits.
PS50157. ZINC_FINGER_C2H2_2. 11 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVDLLAARG TEPVTFRDVA VSFSQDEWLH LDPAQRTLYR EVMLENYSNL
60 70 80 90 100
ASLGFQASIP PVIGKLQKGQ DPCMEREAPE DTCLDFQIQS EIEASSPEQD
110 120 130 140 150
VFIEGPSRGL LKNRSTKCAY WKISFGELVK YERLETAQEQ EKKAHEPGAA
160 170 180 190 200
SPKEVTSEDG IPTDPELEKP LFMNKALVSQ ETDPIERVPG MYHTSEKDLP
210 220 230 240 250
QDFDLMRNFQ IYPGQKPYVC SECGKGFSQS LHLLEHKRIH TGEKPYKCSE
260 270 280 290 300
CGKSFSHRSS LLAHQRTHTG EKPYKCSECE KAFGSSSTLI KHLRVHTGEK
310 320 330 340 350
PYRCRECGKA FSQCSTLTVH QRIHTGEKLY KCAECDKAFN CRAKLHRHQR
360 370 380 390 400
IHTGEKPYKC AECGKGYSQF PSLAEHQRLH TGGQLCQCLQ CGRTFTRVST
410 420 430 440 450
LIEHQRIHTG QKPYQCNECG KTFNQYSSFN EHRKIHTGEK LYTCEECGKA
460 470 480 490 500
FGCKSNLYRH QRIHTGEKPY QCNQCGKAFS QYSFLTEHER IHTGEKLYKC
510 520 530 540 550
MECGKAYSYR SNLCRHKKVH LKERLYKWKE YGTPFMYGSS LAPHQRCLKG
560
EKPEDLNSSL
Length:560
Mass (Da):64,193
Last modified:March 1, 2001 - v1
Checksum:i09249239626F22F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321874 mRNA. Translation: AAG41994.1.
RefSeqiNP_076478.1. NM_023988.1.
UniGeneiRn.230042.

Genome annotation databases

GeneIDi78972.
KEGGirno:78972.
UCSCiRGD:69405. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321874 mRNA. Translation: AAG41994.1.
RefSeqiNP_076478.1. NM_023988.1.
UniGeneiRn.230042.

3D structure databases

ProteinModelPortaliQ9EPU7.
SMRiQ9EPU7. Positions 227-380, 439-520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000248.

Proteomic databases

PaxDbiQ9EPU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi78972.
KEGGirno:78972.
UCSCiRGD:69405. rat.

Organism-specific databases

CTDi30944.
RGDi69405. Zfp354c.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ9EPU7.
KOiK09228.
PhylomeDBiQ9EPU7.

Miscellaneous databases

NextBioi614436.
PROiQ9EPU7.

Family and domain databases

Gene3Di3.30.160.60. 11 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 2 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 11 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 11 hits.
PS50157. ZINC_FINGER_C2H2_2. 11 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of a novel KRAB/C2H2 zinc finger transcription factor involved in bone development."
    Jheon A.H., Ganss B., Cheifetz S., Sodek J.
    J. Biol. Chem. 276:18282-18289(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH RUNX2.
    Strain: Wistar.

Entry informationi

Entry nameiZ354C_RAT
AccessioniPrimary (citable) accession number: Q9EPU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.