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Protein

Cleavage and polyadenylation specificity factor subunit 1

Gene

Cpsf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage and polyadenylation specificity factor subunit 1
Alternative name(s):
Cleavage and polyadenylation specificity factor 160 kDa subunit
Short name:
CPSF 160 kDa subunit
Gene namesi
Name:Cpsf1
Synonyms:Cpsf160
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2679722. Cpsf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14411441Cleavage and polyadenylation specificity factor subunit 1PRO_0000074388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei754 – 7541PhosphoserineCombined sources
Modified residuei764 – 7641PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9EPU4.
MaxQBiQ9EPU4.
PaxDbiQ9EPU4.
PeptideAtlasiQ9EPU4.
PRIDEiQ9EPU4.

PTM databases

iPTMnetiQ9EPU4.
PhosphoSiteiQ9EPU4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034022.
CleanExiMM_CPSF1.
GenevisibleiQ9EPU4. MM.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Found in a complex with CPSF1, FIP1L1 and PAPOLA. Interacts with FIP1L1 and SRRM1. Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of selected pre-mRNAs (By similarity). Interacts with PAPD4/GLD2.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220487. 3 interactions.
IntActiQ9EPU4. 1 interaction.
MINTiMINT-4091937.
STRINGi10090.ENSMUSP00000071794.

Structurei

3D structure databases

ProteinModelPortaliQ9EPU4.
SMRiQ9EPU4. Positions 143-395, 1118-1437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CPSF1 family.Curated

Phylogenomic databases

eggNOGiKOG1896. Eukaryota.
COG5161. LUCA.
GeneTreeiENSGT00550000075040.
HOGENOMiHOG000007904.
HOVERGENiHBG051105.
InParanoidiQ9EPU4.
KOiK14401.
OMAiFIVQVTT.
OrthoDBiEOG091G00LQ.
PhylomeDBiQ9EPU4.
TreeFamiTF314322.

Family and domain databases

InterProiIPR004871. Cleavage/polyA-sp_fac_asu_C.
[Graphical view]
PfamiPF03178. CPSF_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYAVYKQAHP PTGLEFTMYC NFFNNSERNL VVAGTSQLYV YRLNRDAEAL
60 70 80 90 100
TKNDGSTEGK AHREKLELVA SFSFFGNVMS MASVQLAGAK RDALLLSFKD
110 120 130 140 150
AKLSVVEYDP GTHDLKTLSL HYFEEPELRD GFVQNVHTPR VRVDPDGRCA
160 170 180 190 200
AMLIYGTRLV VLPFRRESLA EEHEGLMGEG QRSSFLPSYI IDVRALDEKL
210 220 230 240 250
LNIIDLQFLH GYYEPTLLIL FEPNQTWPGR VAVRQDTCSI VAISLNITQK
260 270 280 290 300
VHPVIWSLTS LPFDCTQALA VPKPIGGVVI FAVNSLLYLN QSVPPYGVAL
310 320 330 340 350
NSLTTGTTAF PLRTQEGVRI TLDCAQAAFI SYDKMVISLK GGEIYVLTLI
360 370 380 390 400
TDGMRSVRAF HFDKAAASVL TTSMVTMEPG YLFLGSRLGN SLLLKYTEKL
410 420 430 440 450
QEPPASSVRE AADKEEPPSK KKRVEPAVGW TGGKTVPQDE VDEIEVYGSE
460 470 480 490 500
AQSGTQLATY SFEVCDSMLN IGPCANAAVG EPAFLSEEFQ NSPEPDLEIV
510 520 530 540 550
VCSGYGKNGA LSVLQKSIRP QVVTTFELPG CYDMWTVIAP VRKEEEETPK
560 570 580 590 600
AESTEQEPSA PKAEEDGRRH GFLILSREDS TMILQTGQEI MELDTSGFAT
610 620 630 640 650
QGPTVFAGNI GDNRYIVQVS PLGIRLLEGV NQLHFIPVDL GAPIVQCAVA
660 670 680 690 700
DPYVVIMSAE GHVTMFLLKS DSYGGRHHRL ALHKPPLHHQ SKVIALCLYR
710 720 730 740 750
DVSGMFTTES RLGGARDELG GRSGSEAEGL GSETSPTVDD EEEMLYGDSS
760 770 780 790 800
ALFSPSKEEA RRSSQPPADR DPAPFKADPT HWCLLVRENG TMEIYQLPDW
810 820 830 840 850
RLVFLVKNFP VGQRVLVDSS FGQPTTQGEV RKEEATRQGE LPLVKEVLLV
860 870 880 890 900
ALGSRQSRPY LLVHVDQELL IYEAFPHDSQ LGQGNLKVRF KKVPHNINFR
910 920 930 940 950
EKKPKPSKKK AEGCSTEEGS GGRGRVARFR YFEDIYGYSG VFICGPSPHW
960 970 980 990 1000
LLVTGRGALR LHPMGIDGPI DSFAPFHNVN CPRGFLYFNR QGELRISVLP
1010 1020 1030 1040 1050
AYLSYDAPWP VRKIPLRCTA HYVAYHVESK VYAVATSTNT PCTRIPRMTG
1060 1070 1080 1090 1100
EEKEFEAIER DDRYIHPQQE AFSIQLISPV SWEAIPNARI ELEEWEHVTC
1110 1120 1130 1140 1150
MKTVSLRSEE TVSGLKGYVA AGTCLMQGEE VTCRGRILIM DVIEVVPEPG
1160 1170 1180 1190 1200
QPLTKNKFKV LYEKEQKGPV TALCHCNGHL VSAIGQKIFL WSLRASELTG
1210 1220 1230 1240 1250
MAFIDTQLYI HQMISVKNFI LAADVMKSIS LLRYQEESKT LSLVSRDAKP
1260 1270 1280 1290 1300
LEVYSVDFMV DNAQLGFLVS DRDRNLMVYM YLPEAKESFG GMRLLRRADF
1310 1320 1330 1340 1350
HVGAHVNTFW RTPCRGAAEG PSKKSVVWEN KHITWFATLD GGIGLLLPMQ
1360 1370 1380 1390 1400
EKTYRRLLML QNALTTMLPH HAGLNPRAFR MLHVDRRILQ NAVRNVLDGE
1410 1420 1430 1440
LLNRYLYLST MERSELAKKI GTTPDIILDD LLETDRVTAH F
Length:1,441
Mass (Da):160,818
Last modified:March 1, 2001 - v1
Checksum:i5D927224152AC3B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322193 mRNA. Translation: AAG40326.1.
BC056388 mRNA. Translation: AAH56388.1.
CCDSiCCDS27577.1.
RefSeqiNP_001157645.1. NM_001164173.1.
NP_444423.1. NM_053193.2.
UniGeneiMm.45141.

Genome annotation databases

EnsembliENSMUST00000071898; ENSMUSP00000071794; ENSMUSG00000034022.
GeneIDi94230.
KEGGimmu:94230.
UCSCiuc007wky.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322193 mRNA. Translation: AAG40326.1.
BC056388 mRNA. Translation: AAH56388.1.
CCDSiCCDS27577.1.
RefSeqiNP_001157645.1. NM_001164173.1.
NP_444423.1. NM_053193.2.
UniGeneiMm.45141.

3D structure databases

ProteinModelPortaliQ9EPU4.
SMRiQ9EPU4. Positions 143-395, 1118-1437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220487. 3 interactions.
IntActiQ9EPU4. 1 interaction.
MINTiMINT-4091937.
STRINGi10090.ENSMUSP00000071794.

PTM databases

iPTMnetiQ9EPU4.
PhosphoSiteiQ9EPU4.

Proteomic databases

EPDiQ9EPU4.
MaxQBiQ9EPU4.
PaxDbiQ9EPU4.
PeptideAtlasiQ9EPU4.
PRIDEiQ9EPU4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071898; ENSMUSP00000071794; ENSMUSG00000034022.
GeneIDi94230.
KEGGimmu:94230.
UCSCiuc007wky.2. mouse.

Organism-specific databases

CTDi29894.
MGIiMGI:2679722. Cpsf1.

Phylogenomic databases

eggNOGiKOG1896. Eukaryota.
COG5161. LUCA.
GeneTreeiENSGT00550000075040.
HOGENOMiHOG000007904.
HOVERGENiHBG051105.
InParanoidiQ9EPU4.
KOiK14401.
OMAiFIVQVTT.
OrthoDBiEOG091G00LQ.
PhylomeDBiQ9EPU4.
TreeFamiTF314322.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

PROiQ9EPU4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034022.
CleanExiMM_CPSF1.
GenevisibleiQ9EPU4. MM.

Family and domain databases

InterProiIPR004871. Cleavage/polyA-sp_fac_asu_C.
[Graphical view]
PfamiPF03178. CPSF_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPSF1_MOUSE
AccessioniPrimary (citable) accession number: Q9EPU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.