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Q9EPU2

- NCOA3_RAT

UniProt

Q9EPU2 - NCOA3_RAT

Protein

Nuclear receptor coactivator 3

Gene

Ncoa3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit By similarity.By similarity

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Enzyme regulationi

    Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones, and the TNF cytokine, respectively. TNF stimulation probably enhances phosphorylation, which in turn activates coactivator function. In contrast, acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors By similarity.By similarity

    GO - Molecular functioni

    1. androgen receptor binding Source: RGD
    2. estrogen receptor binding Source: RGD
    3. histone acetyltransferase activity Source: UniProtKB-EC
    4. ligand-dependent nuclear receptor transcription coactivator activity Source: RGD
    5. peroxisome proliferator activated receptor binding Source: RGD
    6. protein complex binding Source: RGD
    7. RNA polymerase II regulatory region DNA binding Source: RGD
    8. signal transducer activity Source: InterPro

    GO - Biological processi

    1. male gonad development Source: RGD
    2. negative regulation of apoptotic process Source: RGD
    3. positive regulation of cell growth Source: RGD
    4. positive regulation of intracellular estrogen receptor signaling pathway Source: RGD
    5. positive regulation of intracellular steroid hormone receptor signaling pathway Source: RGD
    6. positive regulation of sequence-specific DNA binding transcription factor activity Source: RGD
    7. positive regulation of transcription from RNA polymerase II promoter Source: RGD
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Acyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor coactivator 3 (EC:2.3.1.48)
    Short name:
    NCoA-3
    Alternative name(s):
    Amplified in breast cancer-1 protein homolog
    Short name:
    AIB-1
    Gene namesi
    Name:Ncoa3
    Synonyms:Aib1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620109. Ncoa3.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. Golgi apparatus Source: RGD
    3. nucleus Source: RGD
    4. perinuclear region of cytoplasm Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 1082›1082Nuclear receptor coactivator 3PRO_0000094408Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei251 – 2511PhosphoserineBy similarity
    Modified residuei316 – 3161N6-acetyllysine; by CREBBPBy similarity
    Modified residuei319 – 3191N6-acetyllysine; by CREBBPBy similarity
    Modified residuei320 – 3201N6-acetyllysine; by CREBBPBy similarity
    Modified residuei387 – 3871N6-acetyllysineBy similarity
    Modified residuei427 – 4271PhosphoserineBy similarity
    Modified residuei551 – 5511PhosphoserineBy similarity
    Modified residuei554 – 5541PhosphoserineBy similarity

    Post-translational modificationi

    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function By similarity.By similarity
    Methylated by CARM1.By similarity
    Phosphorylated by IKK complex. Regulated its function By similarity.By similarity

    Keywords - PTMi

    Acetylation, Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9EPU2.
    PRIDEiQ9EPU2.

    PTM databases

    PhosphoSiteiQ9EPU2.

    Expressioni

    Tissue specificityi

    Expressed constitutively in uterus.1 Publication

    Gene expression databases

    GenevestigatoriQ9EPU2.

    Interactioni

    Subunit structurei

    Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP. Interacts with PCAF and CARM1. Interacts with CASP8AP2 and NR3C1 By similarity. Interacts with ATAD2 and this interaction is enhanced by estradiol By similarity. Interacts with PSMB9. Binds to CSNK1D By similarity. Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with NPAS2 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EPU2.
    SMRiQ9EPU2. Positions 734-780.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni712 – 78271Interaction with CREBBPBy similarityAdd
    BLAST
    Regioni786 – 962177AcetyltransferaseAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi385 – 3895LXXLL motif 1
    Motifi437 – 4415LXXLL motif 2
    Motifi723 – 7275LXXLL motif 3

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi136 – 371236Ser-richAdd
    BLAST
    Compositional biasi773 – 936164Gln-richAdd
    BLAST

    Domaini

    Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain) (By similarity).By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG315556.
    HOGENOMiHOG000230947.
    HOVERGENiHBG052583.
    PhylomeDBiQ9EPU2.

    Family and domain databases

    Gene3Di4.10.630.10. 2 hits.
    InterProiIPR010011. DUF1518.
    IPR028818. NCOA3.
    IPR009110. Nuc_rcpt_coact.
    IPR014920. Nuc_rcpt_coact_Ncoa-typ.
    IPR017426. Nuclear_rcpt_coactivator.
    IPR000014. PAS.
    IPR014935. SRC-1.
    IPR008955. Src1_rcpt_coact.
    [Graphical view]
    PANTHERiPTHR10684. PTHR10684. 1 hit.
    PTHR10684:SF3. PTHR10684:SF3. 1 hit.
    PfamiPF07469. DUF1518. 1 hit.
    PF08815. Nuc_rec_co-act. 1 hit.
    PF08832. SRC-1. 1 hit.
    [Graphical view]
    SUPFAMiSSF55785. SSF55785. 1 hit.
    SSF69125. SSF69125. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Q9EPU2-1 [UniParc]FASTAAdd to Basket

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    IIRRCIQRFF SLNDGQSWSQ KRHYQEAYIH GHAETPVYRF SLADGTIVSA     50
    QTKSKLFRNP VTNDRHGFVS THFLQREQNG CRPNPILQDK GIRPPAAGCG 100
    MSLSPSQSVQ MLGSRTYGVA DPSNTGQMAG ARYGASSSVA SLTPGQSLQS 150
    PSSYQSNSYG LNMSSPPHGS PGLGPNQQNI MISPRNRGSP KMASHQFSPA 200
    AGVHSPMGSS GNTGSHSFSS SSLSALQAIS EGVGTSLLST LSSPGPKLDN 250
    SPNMNINQPS KASSQDSKSP LGLYCEQNPV ESSVCPSNSR DPPVTKENKE 300
    NSGEASETPR GPLESKGHKK LLQLLTCSSD DRGHSSLTNS PLDSNCKDSS 350
    ISVTSPSGVS SSTSGAVSST SNMHGSLLQE KHRILHKLLQ NGNSPAEVAK 400
    ITAEATGKDT SSTASGGEGS VXQEQLSPXK KENNALLRYL LDRDDPSDVL 450
    AKELQPQADG GDSKLSQCSC XTNPSSGQEK DPKIKTEEVS GDLDNLDAIL 500
    GDLTSSDFYN SPTNGSHPGA KQQMFAGPSS LGLRSPQPVQ SVRPPYNRAL 550
    SLDSPVSVGS VPPVKNVSAF PVLPKQPILA GNPRMMDSQE NYGANMGGPN 600
    RNVPVNPTSS SGDWGLANSR ASRMEPLASS PLGRAGGDYS AALPRPALGS 650
    SGPTLPLRSN RLPGARPTLM LQMRAGEVPM GMGVSPYSPA VPSNQPGSWP 700
    EGMLSMEQGP HGAQNRPLLR NSLDELLGPP SNPEGQSDER ALLDQLHTLL 750
    SNTDATGLEE IDRALGIPEL VSQGQALESK QDVFQGQEAA VMMDQKAALY 800
    GQTYPAQGPP LQGGFHLQGQ SPSLNSMMSQ ISQQGSFPLQ GLHPRASMVR 850
    PRTNTPKQLR MQLQQRLQGQ QFLNQSRQAL EMKMESPTGA AVMRPMLQSQ 900
    QAFFNAQMAA QQKRELMNHH LQQQRMAMMM SQPQPQAFSP PPNVTASPSM 950
    DGVLAGSAMP QAPPQQFPYA TNYGMGQPPE PAFGRGSSPP SAMMSSRMGP 1000
    SQNAMVQHPQ TAPMYQSSEM KGWPSGNLAR NGSFPQQQFA PQANPAAYNM 1050
    VHMNSSGSHL GQMTMTPMPM SGMPMGPDQK YC 1082
    Length:1,082
    Mass (Da):115,134
    Last modified:March 1, 2001 - v1
    Checksum:iBA35D946CBC8C080
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF322224 mRNA. Translation: AAG42837.1.
    UniGeneiRn.20691.

    Genome annotation databases

    UCSCiRGD:620109. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF322224 mRNA. Translation: AAG42837.1 .
    UniGenei Rn.20691.

    3D structure databases

    ProteinModelPortali Q9EPU2.
    SMRi Q9EPU2. Positions 734-780.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9EPU2.

    Proteomic databases

    PaxDbi Q9EPU2.
    PRIDEi Q9EPU2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:620109. rat.

    Organism-specific databases

    RGDi 620109. Ncoa3.

    Phylogenomic databases

    eggNOGi NOG315556.
    HOGENOMi HOG000230947.
    HOVERGENi HBG052583.
    PhylomeDBi Q9EPU2.

    Miscellaneous databases

    PROi Q9EPU2.

    Gene expression databases

    Genevestigatori Q9EPU2.

    Family and domain databases

    Gene3Di 4.10.630.10. 2 hits.
    InterProi IPR010011. DUF1518.
    IPR028818. NCOA3.
    IPR009110. Nuc_rcpt_coact.
    IPR014920. Nuc_rcpt_coact_Ncoa-typ.
    IPR017426. Nuclear_rcpt_coactivator.
    IPR000014. PAS.
    IPR014935. SRC-1.
    IPR008955. Src1_rcpt_coact.
    [Graphical view ]
    PANTHERi PTHR10684. PTHR10684. 1 hit.
    PTHR10684:SF3. PTHR10684:SF3. 1 hit.
    Pfami PF07469. DUF1518. 1 hit.
    PF08815. Nuc_rec_co-act. 1 hit.
    PF08832. SRC-1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55785. SSF55785. 1 hit.
    SSF69125. SSF69125. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression of estrogen receptor coactivators in the rat uterus."
      Nephew K.P., Ray S., Hlaing M., Ahluwalia A., Wu S.D., Long X., Hyder S.M., Bigsby R.M.
      Biol. Reprod. 63:361-367(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiNCOA3_RAT
    AccessioniPrimary (citable) accession number: Q9EPU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3