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Q9EPU2

- NCOA3_RAT

UniProt

Q9EPU2 - NCOA3_RAT

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Protein
Nuclear receptor coactivator 3
Gene
Ncoa3, Aib1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit By similarity.

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Enzyme regulationi

Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones, and the TNF cytokine, respectively. TNF stimulation probably enhances phosphorylation, which in turn activates coactivator function. In contrast, acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors By similarity.

GO - Molecular functioni

  1. RNA polymerase II regulatory region DNA binding Source: RGD
  2. androgen receptor binding Source: RGD
  3. estrogen receptor binding Source: RGD
  4. histone acetyltransferase activity Source: UniProtKB-EC
  5. ligand-dependent nuclear receptor transcription coactivator activity Source: RGD
  6. peroxisome proliferator activated receptor binding Source: RGD
  7. protein complex binding Source: RGD
  8. signal transducer activity Source: InterPro

GO - Biological processi

  1. male gonad development Source: RGD
  2. negative regulation of apoptotic process Source: RGD
  3. positive regulation of cell growth Source: RGD
  4. positive regulation of intracellular estrogen receptor signaling pathway Source: RGD
  5. positive regulation of intracellular steroid hormone receptor signaling pathway Source: RGD
  6. positive regulation of sequence-specific DNA binding transcription factor activity Source: RGD
  7. positive regulation of transcription from RNA polymerase II promoter Source: RGD
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 3 (EC:2.3.1.48)
Short name:
NCoA-3
Alternative name(s):
Amplified in breast cancer-1 protein homolog
Short name:
AIB-1
Gene namesi
Name:Ncoa3
Synonyms:Aib1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620109. Ncoa3.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: RGD
  2. cytoplasm Source: RGD
  3. nucleus Source: RGD
  4. perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 1082›1082Nuclear receptor coactivator 3
PRO_0000094408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei251 – 2511Phosphoserine By similarity
Modified residuei316 – 3161N6-acetyllysine; by CREBBP By similarity
Modified residuei319 – 3191N6-acetyllysine; by CREBBP By similarity
Modified residuei320 – 3201N6-acetyllysine; by CREBBP By similarity
Modified residuei387 – 3871N6-acetyllysine By similarity
Modified residuei427 – 4271Phosphoserine By similarity
Modified residuei551 – 5511Phosphoserine By similarity
Modified residuei554 – 5541Phosphoserine By similarity

Post-translational modificationi

Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function By similarity.
Methylated by CARM1 By similarity.
Phosphorylated by IKK complex. Regulated its function By similarity.

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9EPU2.
PRIDEiQ9EPU2.

PTM databases

PhosphoSiteiQ9EPU2.

Expressioni

Tissue specificityi

Expressed constitutively in uterus.1 Publication

Gene expression databases

GenevestigatoriQ9EPU2.

Interactioni

Subunit structurei

Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP. Interacts with PCAF and CARM1. Interacts with CASP8AP2 and NR3C1 By similarity. Interacts with ATAD2 and this interaction is enhanced by estradiol By similarity. Interacts with PSMB9. Binds to CSNK1D By similarity. Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with NPAS2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9EPU2.
SMRiQ9EPU2. Positions 734-780.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni712 – 78271Interaction with CREBBP By similarity
Add
BLAST
Regioni786 – 962177Acetyltransferase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi385 – 3895LXXLL motif 1
Motifi437 – 4415LXXLL motif 2
Motifi723 – 7275LXXLL motif 3

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 371236Ser-rich
Add
BLAST
Compositional biasi773 – 936164Gln-rich
Add
BLAST

Domaini

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain) (By similarity).

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG315556.
HOGENOMiHOG000230947.
HOVERGENiHBG052583.
PhylomeDBiQ9EPU2.

Family and domain databases

Gene3Di4.10.630.10. 2 hits.
InterProiIPR010011. DUF1518.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PANTHERiPTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
PfamiPF07469. DUF1518. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF69125. SSF69125. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q9EPU2-1 [UniParc]FASTAAdd to Basket

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IIRRCIQRFF SLNDGQSWSQ KRHYQEAYIH GHAETPVYRF SLADGTIVSA     50
QTKSKLFRNP VTNDRHGFVS THFLQREQNG CRPNPILQDK GIRPPAAGCG 100
MSLSPSQSVQ MLGSRTYGVA DPSNTGQMAG ARYGASSSVA SLTPGQSLQS 150
PSSYQSNSYG LNMSSPPHGS PGLGPNQQNI MISPRNRGSP KMASHQFSPA 200
AGVHSPMGSS GNTGSHSFSS SSLSALQAIS EGVGTSLLST LSSPGPKLDN 250
SPNMNINQPS KASSQDSKSP LGLYCEQNPV ESSVCPSNSR DPPVTKENKE 300
NSGEASETPR GPLESKGHKK LLQLLTCSSD DRGHSSLTNS PLDSNCKDSS 350
ISVTSPSGVS SSTSGAVSST SNMHGSLLQE KHRILHKLLQ NGNSPAEVAK 400
ITAEATGKDT SSTASGGEGS VXQEQLSPXK KENNALLRYL LDRDDPSDVL 450
AKELQPQADG GDSKLSQCSC XTNPSSGQEK DPKIKTEEVS GDLDNLDAIL 500
GDLTSSDFYN SPTNGSHPGA KQQMFAGPSS LGLRSPQPVQ SVRPPYNRAL 550
SLDSPVSVGS VPPVKNVSAF PVLPKQPILA GNPRMMDSQE NYGANMGGPN 600
RNVPVNPTSS SGDWGLANSR ASRMEPLASS PLGRAGGDYS AALPRPALGS 650
SGPTLPLRSN RLPGARPTLM LQMRAGEVPM GMGVSPYSPA VPSNQPGSWP 700
EGMLSMEQGP HGAQNRPLLR NSLDELLGPP SNPEGQSDER ALLDQLHTLL 750
SNTDATGLEE IDRALGIPEL VSQGQALESK QDVFQGQEAA VMMDQKAALY 800
GQTYPAQGPP LQGGFHLQGQ SPSLNSMMSQ ISQQGSFPLQ GLHPRASMVR 850
PRTNTPKQLR MQLQQRLQGQ QFLNQSRQAL EMKMESPTGA AVMRPMLQSQ 900
QAFFNAQMAA QQKRELMNHH LQQQRMAMMM SQPQPQAFSP PPNVTASPSM 950
DGVLAGSAMP QAPPQQFPYA TNYGMGQPPE PAFGRGSSPP SAMMSSRMGP 1000
SQNAMVQHPQ TAPMYQSSEM KGWPSGNLAR NGSFPQQQFA PQANPAAYNM 1050
VHMNSSGSHL GQMTMTPMPM SGMPMGPDQK YC 1082
Length:1,082
Mass (Da):115,134
Last modified:March 1, 2001 - v1
Checksum:iBA35D946CBC8C080
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF322224 mRNA. Translation: AAG42837.1.
UniGeneiRn.20691.

Genome annotation databases

UCSCiRGD:620109. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF322224 mRNA. Translation: AAG42837.1 .
UniGenei Rn.20691.

3D structure databases

ProteinModelPortali Q9EPU2.
SMRi Q9EPU2. Positions 734-780.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9EPU2.

Proteomic databases

PaxDbi Q9EPU2.
PRIDEi Q9EPU2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620109. rat.

Organism-specific databases

RGDi 620109. Ncoa3.

Phylogenomic databases

eggNOGi NOG315556.
HOGENOMi HOG000230947.
HOVERGENi HBG052583.
PhylomeDBi Q9EPU2.

Miscellaneous databases

PROi Q9EPU2.

Gene expression databases

Genevestigatori Q9EPU2.

Family and domain databases

Gene3Di 4.10.630.10. 2 hits.
InterProi IPR010011. DUF1518.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view ]
PANTHERi PTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
Pfami PF07469. DUF1518. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 1 hit.
SSF69125. SSF69125. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Expression of estrogen receptor coactivators in the rat uterus."
    Nephew K.P., Ray S., Hlaing M., Ahluwalia A., Wu S.D., Long X., Hyder S.M., Bigsby R.M.
    Biol. Reprod. 63:361-367(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiNCOA3_RAT
AccessioniPrimary (citable) accession number: Q9EPU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3