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Protein

Chloride intracellular channel protein 5

Gene

Clic5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal hearing. It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti. Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (By similarity).By similarity

GO - Molecular functioni

  • chloride channel activity Source: RGD
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Hearing, Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 5
Gene namesi
Name:Clic5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi620659. Clic5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei34 – 5421Helical; Note=After insertion into the membraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Chloride intracellular channel protein 5PRO_0000144216Add
BLAST

Proteomic databases

PaxDbiQ9EPT8.
PRIDEiQ9EPT8.

PTM databases

iPTMnetiQ9EPT8.
PhosphoSiteiQ9EPT8.

Expressioni

Tissue specificityi

Detected in cochlea, in cochlear and vestibular hair cell bundles in the organ of Corti (at protein level).1 Publication

Gene expression databases

GenevisibleiQ9EPT8. RN.

Interactioni

Subunit structurei

Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1.By similarity

Protein-protein interaction databases

BioGridi250191. 1 interaction.
STRINGi10116.ENSRNOP00000067890.

Structurei

3D structure databases

ProteinModelPortaliQ9EPT8.
SMRiQ9EPT8. Positions 10-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 241141GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9898Required for insertion into the membraneBy similarityAdd
BLAST

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated
Contains 1 GST C-terminal domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ9EPT8.
KOiK05025.
OMAiANGDDRD.
OrthoDBiEOG7X3QR3.
PhylomeDBiQ9EPT8.
TreeFamiTF315438.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030264. CLIC-5.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF152. PTHR11260:SF152. 2 hits.
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSATANGD DRDPEIELFV KAGIDGESIG NCPFSQRLFM ILWLKGVVFN
60 70 80 90 100
VTTVDLKRKP ADLHNLAPGT HPPFLTFNGD VKTDVNKIEE FLEETLTPEK
110 120 130 140 150
YPKLAARHRE SNTAGIDIFS KFSAYIKNTK QQNNAALERG LTKALRKLDD
160 170 180 190 200
YLNTPLPEEI DTNTHGDEKG SQRKFLDGDE LTLADCNLLP KLHVVKIVAK
210 220 230 240 250
KYRNYDIPAE MTGLWRYLKN AYARDEFTNT CAADSEIELA YADVARRLSR

S
Length:251
Mass (Da):28,299
Last modified:March 1, 2001 - v1
Checksum:i11079FDE8508D53C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF323174 mRNA. Translation: AAG49367.1.
RefSeqiNP_446055.1. NM_053603.2.
UniGeneiRn.1838.

Genome annotation databases

EnsembliENSRNOT00000073667; ENSRNOP00000067890; ENSRNOG00000047218.
ENSRNOT00000079517; ENSRNOP00000072035; ENSRNOG00000047218.
GeneIDi94272.
KEGGirno:94272.
UCSCiRGD:620659. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF323174 mRNA. Translation: AAG49367.1.
RefSeqiNP_446055.1. NM_053603.2.
UniGeneiRn.1838.

3D structure databases

ProteinModelPortaliQ9EPT8.
SMRiQ9EPT8. Positions 10-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250191. 1 interaction.
STRINGi10116.ENSRNOP00000067890.

PTM databases

iPTMnetiQ9EPT8.
PhosphoSiteiQ9EPT8.

Proteomic databases

PaxDbiQ9EPT8.
PRIDEiQ9EPT8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073667; ENSRNOP00000067890; ENSRNOG00000047218.
ENSRNOT00000079517; ENSRNOP00000072035; ENSRNOG00000047218.
GeneIDi94272.
KEGGirno:94272.
UCSCiRGD:620659. rat.

Organism-specific databases

CTDi53405.
RGDi620659. Clic5.

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ9EPT8.
KOiK05025.
OMAiANGDDRD.
OrthoDBiEOG7X3QR3.
PhylomeDBiQ9EPT8.
TreeFamiTF315438.

Miscellaneous databases

NextBioi617913.
PROiQ9EPT8.

Gene expression databases

GenevisibleiQ9EPT8. RN.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030264. CLIC-5.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF152. PTHR11260:SF152. 2 hits.
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of a rat homolog of the human chloride intracellular channel (CLIC5)."
    Vanderbilt J., Mager E., Dobbs L.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Lung.
  2. "The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function."
    Gagnon L.H., Longo-Guess C.M., Berryman M., Shin J.-B., Saylor K.W., Yu H., Gillespie P.G., Johnson K.R.
    J. Neurosci. 26:10188-10198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCLIC5_RAT
AccessioniPrimary (citable) accession number: Q9EPT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.