Reviewed, UniProtKB/Swiss-Prot Q9EPT4 (CP11A_MESAU)

Names and origin

Protein names

Recommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    Cytochrome P450 11A1
    CYPXIA1
    P450(scc)
    Cholesterol desmolase

Gene names

Name:	CYP11A1
Synonyms:	CYP11A

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone By similarity.
Catalytic activity	Cholesterol + reduced adrenal ferredoxin + O2 = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol monooxygenase (side-chain-cleaving) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Mitochondrion By similarity
	Chain	37 – 520	484	Cholesterol side-chain cleavage enzyme, mitochondrial		PRO_0000003586
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9EPT4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 648C5A82D0D41D1E	FASTA	520	60,271
	10 20 30 40 50 60 MLAKGLSLRS VLAKGCQPFL SPTWQSSVLA TGGGANISTN SPRPFNEIPS PGDNGWLNLY 70 80 90 100 110 120 HFWRENGTHR IHYHHMQNFQ KYGPIYREKL GNKDSVYILD PEDAAQLFLS EGPYPERYLV 130 140 150 160 170 180 PPWVAYHQYY KRPIGVLFKS SEAWKKDRLV LNQEVMAPEA IKNFVPLLEG VVQDFINVLH 190 200 210 220 230 240 RRIKQQKSGN FSGDISDDLF RFAFESITSV VFGERLGMLE EIVDPESQRF IDAIYQMFHT 250 260 270 280 290 300 SVPMLNLPPE LFRFFRTKTW KEHAAAWDMI FKKADDYTQT FYWDLRQKQE FSKYPGVLYS 310 320 330 340 350 360 LLGGNKLPFK NIQANITEML AGGVDTTSMT LQWSLYEMAH NLKVQEMLRA EVLAARRQAQ 370 380 390 400 410 420 GDMVKMVQLV PLLKASIKET LRLHPISVTV QRYLVDDLVL RNYRIPAKML VQVANYAMGR 430 440 450 460 470 480 EPSFFPNPNK FDPTRWLEKS KNTTHFRYLS FGWGVRQCLG RRIAELEMTI FLINVLENFR 490 500 510 520 IELQSLHDVG TKFNLILMPE KPILFNLQPL KKDLGTTTNR

References

[1]	"The cDNA cloning and tissue expression of the cytochrome P450scc from Syrian hamster (Mesocricetus auratus)." Vilchis F., Chavez B., Larrea F., Timossi C., Montiel F. Gen. Comp. Endocrinol. 126:279-286(2002) [PubMed: 12093115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF323965 mRNA. Translation: AAG42320.1.
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9EPT4.
Enzyme and pathway databases
BRENDA	1.14.15.6. 824.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP11A_MESAU

Accession

Primary (citable) accession number: Q9EPT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	March 1, 2001
Last modified:	May 26, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)