Q9EPR4 (S23A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 23 member 2

Alternative name(s):
Na(+)/L-ascorbic acid transporter 2
Sodium-dependent vitamin C transporter 2
Short name=SVCT-2
Short name=mSVCT2
Yolk sac permease-like molecule 2

Gene names

Name:	Slc23a2
Synonyms:	Kiaa0238, Svct2, Yspl2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na+ for each ascorbate By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in metabolically active and specialized tissues, including high expression in brain and adrenals. Detected in a wide range of tissues. Expression in kidney is almost undetectable. Ref.9
Involvement in disease	Elevated expression levels in the adrenals of diabetic mice. Ref.9
Sequence similarities	Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. [View classification]
Sequence caution	The sequence AAG02252.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA90751.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC65509.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Symport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-ascorbic acid metabolic process Inferred from electronic annotation. Source: Compara L-ascorbic acid transport Inferred from direct assay PubMed 18668520. Source: UniProtKB response to oxidative stress Inferred from electronic annotation. Source: Compara transepithelial L-ascorbic acid transport Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara basal plasma membrane Inferred from direct assay PubMed 18668520. Source: UniProtKB cytoplasm Inferred from direct assay PubMed 18668520. Source: UniProtKB integral to plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	L-ascorbate:sodium symporter activity Inferred from mutant phenotype PubMed 11984597. Source: MGI L-ascorbic acid transporter activity Inferred from direct assay PubMed 18668520. Source: UniProtKB sodium-dependent L-ascorbate transmembrane transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9EPR4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9EPR4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-272: LPLFQASAFA...RAGKHWGIAM → DYSCQWNGRA...SLVEYLKQSH
     273-648: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Solute carrier family 23 member 2		PRO_0000165979
Regions
	Transmembrane	103 – 123	21	Helical; Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Transmembrane	240 – 260	21	Helical; Potential
	Transmembrane	264 – 284	21	Helical; Potential
	Transmembrane	311 – 331	21	Helical; Potential
	Transmembrane	370 – 390	21	Helical; Potential
	Transmembrane	460 – 480	21	Helical; Potential
	Transmembrane	484 – 504	21	Helical; Potential
	Transmembrane	512 – 532	21	Helical; Potential
	Transmembrane	546 – 566	21	Helical; Potential
Amino acid modifications
	Modified residue	78	1	Phosphoserine Ref.8
	Modified residue	79	1	Phosphothreonine Ref.11
	Modified residue	81	1	Phosphoserine Ref.10
	Modified residue	647	1	Phosphothreonine Ref.11
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	162 – 272	111	LPLFQ…WGIAM → DYSCQWNGRAVGTHLASPNP RDPGGYHHVLTDRSGHWPPW PAWGSAEVYWTLDHHTHRGP HWPLWFPGSRRESRKALGHC HAVSVLRELQGWGTIFTTMW DSLVEYLKQSH in isoform 2.		VSP_007367
	Alternative sequence	273 – 648	376	Missing in isoform 2.		VSP_007368

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 2590BB11B2A257CB	FASTA	648	70,049
	10 20 30 40 50 60 MMGIGKNTAS KSVEAGGSTE GKYEEEAKHS NFFTLPVVIN GGATSSGEQD NEDTELMAIY 70 80 90 100 110 120 TTENGIAEKS SLAETLDSTG SLDPQRSDMI YTIEDVPPWY LCIFLGLQHY LTCFSGTIAV 130 140 150 160 170 180 PFLLADAMCV GDDQWATSQL IGTIFFCVGI TTLLQTTFGC RLPLFQASAF AFLAPARAIL 190 200 210 220 230 240 SLDKWKCNTT EITVANGTAE LLEHIWHPRI QEIQGAIIMS SLIEVVIGLL GLPGALLRYI 250 260 270 280 290 300 GPLTITPTVA LIGLSGFQAA GERAGKHWGI AMLTIFLVLL FSQYARNVKF PLPIYKSKKG 310 320 330 340 350 360 WTAYKFQLFK MFPIILAILV SWLLCFIFTV TDVFPSNSTD YGYYARTDAR KGVLLVAPWF 370 380 390 400 410 420 KVPYPFQWGM PTVSAAGVIG MLSAVVASII ESIGDYYACA RLSCAPPPPI HAINRGIFVE 430 440 450 460 470 480 GLSCVLDGIF GTGNGSTSSS PNIGVLGITK VGSRRVIQYG AALMLGLGMV GKFSALFASL 490 500 510 520 530 540 PDPVLGALFC TLFGMITAVG LSNLQFIDLN SSRNLFVLGF SIFFGLVLPS YLRQNPLVTG 550 560 570 580 590 600 ITGIDQILNV LLTTAMFVGG CVAFILDNTI PGTPEERGIK KWKKGVSKGS KSLDGMESYN 610 620 630 640 LPFGMNIIKK YRCFSYLPIS PTFAGYTWKG FGKSENSRSS DKDSQATV
	Isoform 2 [UniParc]. Checksum: 1C8D45706B56F5BC	FASTA	272	30,188
	10 20 30 40 50 60 MMGIGKNTAS KSVEAGGSTE GKYEEEAKHS NFFTLPVVIN GGATSSGEQD NEDTELMAIY 70 80 90 100 110 120 TTENGIAEKS SLAETLDSTG SLDPQRSDMI YTIEDVPPWY LCIFLGLQHY LTCFSGTIAV 130 140 150 160 170 180 PFLLADAMCV GDDQWATSQL IGTIFFCVGI TTLLQTTFGC RDYSCQWNGR AVGTHLASPN 190 200 210 220 230 240 PRDPGGYHHV LTDRSGHWPP WPAWGSAEVY WTLDHHTHRG PHWPLWFPGS RRESRKALGH 250 260 270 CHAVSVLREL QGWGTIFTTM WDSLVEYLKQ SH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and genomic organization of the mouse gene slc23a1 encoding a vitamin C transporter." Gispert S., Dutra A., Lieberman A., Friedlich D., Nussbaum R.L. DNA Res. 7:339-345(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: 129/SvEv. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Lung.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo.
[6]	"Mouse sodium dependent vitamin C transporter 2 (mSVCT2)." Fujita I., Hirano J., Tanaka K. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-647 (ISOFORM 1). Tissue: Brain.
[7]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 108-648 (ISOFORM 1). Tissue: Brain.
[8]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78, MASS SPECTROMETRY. Tissue: Forebrain.
[9]	"Upregulation of sodium-dependent vitamin C transporter 2 expression in adrenals increases norepinephrine production and aggravates hyperlipidemia in mice with streptozotocin-induced diabetes." Wu X., Iguchi T., Hirano J., Fujita I., Ueda H., Itoh N., Tanaka K., Nakanishi T. Biochem. Pharmacol. 74:1020-1028(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DISEASE.
[10]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, MASS SPECTROMETRY. Tissue: Melanoma.
[11]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-79 AND THR-647, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY004874 mRNA. Translation: AAG02252.1. Different initiation. AK087175 mRNA. Translation: BAC39819.1. AL831706 Genomic DNA. Translation: CAM26800.1. CH466519 Genomic DNA. Translation: EDL28341.1. BC050823 mRNA. Translation: AAH50823.1. AB038145 mRNA. Translation: BAA90751.1. Different initiation. AK122227 Transcribed RNA. Translation: BAC65509.1. Sequence problems.
IPI	IPI00165688. IPI00261336.
RefSeq	NP_061294.2. NM_018824.2.
UniGene	Mm.103581.
3D structure databases
ProteinModelPortal	Q9EPR4.
SMR	Q9EPR4. Positions 93-526.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000028815.
PTM databases
PhosphoSite	Q9EPR4.
Proteomic databases
PaxDb	Q9EPR4.
PRIDE	Q9EPR4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028815; ENSMUSP00000028815; ENSMUSG00000027340.
GeneID	54338.
KEGG	mmu:54338.
UCSC	uc008mmi.1. mouse. uc008mmj.1. mouse.
Organism-specific databases
CTD	9962.
MGI	MGI:1859682. Slc23a2.
Rouge	Search...
Phylogenomic databases
eggNOG	COG2233.
GeneTree	ENSGT00390000015686.
HOGENOM	HOG000038201.
HOVERGEN	HBG056256.
InParanoid	Q80Y23.
KO	K14611.
OMA	LEKWKCN.
OrthoDB	EOG4JHCF8.
Gene expression databases
ArrayExpress	Q9EPR4.
Bgee	Q9EPR4.
Genevestigator	Q9EPR4.
GermOnline	ENSMUSG00000027340. Mus musculus.
Family and domain databases
InterPro	IPR006042. Xan_ur_permease. IPR006043. Xant/urac/vitC. [Graphical view]
PANTHER	PTHR11119. PTHR11119. 1 hit.
Pfam	PF00860. Xan_ur_permease. 1 hit. [Graphical view]
PROSITE	PS01116. XANTH_URACIL_PERMASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	311116.
SOURCE	Search...

Entry information

Entry name

S23A2_MOUSE

Accession

Primary (citable) accession number: Q9EPR4
Secondary accession number(s): Q80Y23, Q8C327, Q9JM78

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	September 22, 2009
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program