Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9EPR4 (S23A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 23 member 2
Alternative name(s):
Na(+)/L-ascorbic acid transporter 2
Sodium-dependent vitamin C transporter 2
Short name=SVCT-2
Short name=mSVCT2
Yolk sac permease-like molecule 2
Gene names
Name:Slc23a2
Synonyms:Kiaa0238, Svct2, Yspl2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na+ for each ascorbate By similarity.

Subcellular location

Cell membrane By similarity; Multi-pass membrane protein.

Tissue specificity

Expressed in metabolically active and specialized tissues, including high expression in brain and adrenals. Detected in a wide range of tissues. Expression in kidney is almost undetectable. Ref.8

Involvement in disease

Elevated expression levels in the adrenals of diabetic mice. Ref.8

Sequence similarities

Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. [View classification]

Sequence caution

The sequence AAG02252.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA90751.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC65509.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9EPR4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9EPR4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-272: LPLFQASAFA...RAGKHWGIAM → DYSCQWNGRA...SLVEYLKQSH
     273-648: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648Solute carrier family 23 member 2
PRO_0000165979

Regions

Topological domain9 – 110102Cytoplasmic Potential
Transmembrane111 – 13121Helical; Potential
Topological domain132 – 1398Extracellular Potential
Transmembrane140 – 16021Helical; Potential
Topological domain1611Cytoplasmic Potential
Transmembrane162 – 18221Helical; Potential
Topological domain183 – 21634Extracellular Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 26427Cytoplasmic Potential
Transmembrane265 – 28218Helical; Potential
Topological domain283 – 2864Extracellular Potential
Intramembrane287 – 30014Helical; Potential
Topological domain301 – 3077Extracellular Potential
Transmembrane308 – 32821Helical; Potential
Topological domain329 – 36941Cytoplasmic Potential
Transmembrane370 – 39021Helical; Potential
Topological domain391 – 41525Extracellular Potential
Transmembrane416 – 43621Helical; Potential
Topological domain437 – 45923Cytoplasmic Potential
Transmembrane460 – 48021Helical; Potential
Topological domain481 – 4833Extracellular Potential
Transmembrane484 – 50421Helical; Potential
Topological domain505 – 51410Cytoplasmic Potential
Transmembrane515 – 53521Helical; Potential
Topological domain536 – 54510Extracellular Potential
Transmembrane546 – 56621Helical; Potential
Topological domain567 – 64882Cytoplasmic Potential

Amino acid modifications

Modified residue701Phosphoserine Ref.9
Modified residue781Phosphoserine Ref.9
Modified residue791Phosphothreonine Ref.9
Modified residue811Phosphoserine Ref.9
Modified residue6471Phosphothreonine Ref.9
Glycosylation1881N-linked (GlcNAc...) Potential
Glycosylation1961N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence162 – 272111LPLFQ…WGIAM → DYSCQWNGRAVGTHLASPNP RDPGGYHHVLTDRSGHWPPW PAWGSAEVYWTLDHHTHRGP HWPLWFPGSRRESRKALGHC HAVSVLRELQGWGTIFTTMW DSLVEYLKQSH in isoform 2.
VSP_007367
Alternative sequence273 – 648376Missing in isoform 2.
VSP_007368

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 2590BB11B2A257CB

FASTA64870,049
        10         20         30         40         50         60 
MMGIGKNTAS KSVEAGGSTE GKYEEEAKHS NFFTLPVVIN GGATSSGEQD NEDTELMAIY 

        70         80         90        100        110        120 
TTENGIAEKS SLAETLDSTG SLDPQRSDMI YTIEDVPPWY LCIFLGLQHY LTCFSGTIAV 

       130        140        150        160        170        180 
PFLLADAMCV GDDQWATSQL IGTIFFCVGI TTLLQTTFGC RLPLFQASAF AFLAPARAIL 

       190        200        210        220        230        240 
SLDKWKCNTT EITVANGTAE LLEHIWHPRI QEIQGAIIMS SLIEVVIGLL GLPGALLRYI 

       250        260        270        280        290        300 
GPLTITPTVA LIGLSGFQAA GERAGKHWGI AMLTIFLVLL FSQYARNVKF PLPIYKSKKG 

       310        320        330        340        350        360 
WTAYKFQLFK MFPIILAILV SWLLCFIFTV TDVFPSNSTD YGYYARTDAR KGVLLVAPWF 

       370        380        390        400        410        420 
KVPYPFQWGM PTVSAAGVIG MLSAVVASII ESIGDYYACA RLSCAPPPPI HAINRGIFVE 

       430        440        450        460        470        480 
GLSCVLDGIF GTGNGSTSSS PNIGVLGITK VGSRRVIQYG AALMLGLGMV GKFSALFASL 

       490        500        510        520        530        540 
PDPVLGALFC TLFGMITAVG LSNLQFIDLN SSRNLFVLGF SIFFGLVLPS YLRQNPLVTG 

       550        560        570        580        590        600 
ITGIDQILNV LLTTAMFVGG CVAFILDNTI PGTPEERGIK KWKKGVSKGS KSLDGMESYN 

       610        620        630        640 
LPFGMNIIKK YRCFSYLPIS PTFAGYTWKG FGKSENSRSS DKDSQATV 

« Hide

Isoform 2 [UniParc].

Checksum: 1C8D45706B56F5BC
Show »

FASTA27230,188

References

« Hide 'large scale' references
[1]"Cloning and genomic organization of the mouse gene slc23a1 encoding a vitamin C transporter."
Gispert S., Dutra A., Lieberman A., Friedlich D., Nussbaum R.L.
DNA Res. 7:339-345(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: 129/SvEv.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Lung.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Embryo.
[6]"Mouse sodium dependent vitamin C transporter 2 (mSVCT2)."
Fujita I., Hirano J., Tanaka K.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-647 (ISOFORM 1).
Tissue: Brain.
[7]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 108-648 (ISOFORM 1).
Tissue: Brain.
[8]"Upregulation of sodium-dependent vitamin C transporter 2 expression in adrenals increases norepinephrine production and aggravates hyperlipidemia in mice with streptozotocin-induced diabetes."
Wu X., Iguchi T., Hirano J., Fujita I., Ueda H., Itoh N., Tanaka K., Nakanishi T.
Biochem. Pharmacol. 74:1020-1028(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DISEASE.
[9]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70; SER-78; THR-79; SER-81 AND THR-647, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY004874 mRNA. Translation: AAG02252.1. Different initiation.
AK087175 mRNA. Translation: BAC39819.1.
AL831706 Genomic DNA. Translation: CAM26800.1.
CH466519 Genomic DNA. Translation: EDL28341.1.
BC050823 mRNA. Translation: AAH50823.1.
AB038145 mRNA. Translation: BAA90751.1. Different initiation.
AK122227 Transcribed RNA. Translation: BAC65509.1. Sequence problems.
RefSeqNP_061294.2. NM_018824.2.
XP_006499969.1. XM_006499906.1.
XP_006499970.1. XM_006499907.1.
UniGeneMm.103581.

3D structure databases

ProteinModelPortalQ9EPR4.
SMRQ9EPR4. Positions 90-571.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000028815.

PTM databases

PhosphoSiteQ9EPR4.

Proteomic databases

PaxDbQ9EPR4.
PRIDEQ9EPR4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028815; ENSMUSP00000028815; ENSMUSG00000027340. [Q9EPR4-1]
GeneID54338.
KEGGmmu:54338.
UCSCuc008mmi.1. mouse. [Q9EPR4-1]
uc008mmj.1. mouse. [Q9EPR4-2]

Organism-specific databases

CTD9962.
MGIMGI:1859682. Slc23a2.
RougeSearch...

Phylogenomic databases

eggNOGCOG2233.
GeneTreeENSGT00390000015686.
HOGENOMHOG000038201.
HOVERGENHBG056256.
InParanoidQ80Y23.
KOK14611.
OMAKHPTFFT.
OrthoDBEOG7R56RZ.
PhylomeDBQ9EPR4.
TreeFamTF313272.

Gene expression databases

ArrayExpressQ9EPR4.
BgeeQ9EPR4.
GenevestigatorQ9EPR4.

Family and domain databases

InterProIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERPTHR11119. PTHR11119. 1 hit.
PfamPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
PROSITEPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311116.
PROQ9EPR4.
SOURCESearch...

Entry information

Entry nameS23A2_MOUSE
AccessionPrimary (citable) accession number: Q9EPR4
Secondary accession number(s): Q80Y23, Q8C327, Q9JM78
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: September 22, 2009
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot