Q9EPR2 (PG12A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Group XIIA secretory phospholipase A2
Short name=GXII sPLA2
Short name=sPLA2-XII
EC=3.1.1.4

Alternative name(s):
Phosphatidylcholine 2-acylhydrolase 12A

Gene names

Name:	Pla2g12a
Synonyms:	Pla2g12

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted. Cytoplasm By similarity.
Sequence similarities	Belongs to the phospholipase A2 family.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cytoplasm Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred by curator Ref.1. Source: MGI phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay Ref.1. Source: MGI endoplasmic reticulum Inferred from direct assay Ref.1. Source: MGI extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9EPR2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9EPR2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MVTPRPAPAR...GLCQYKCSDG → MKDYHSGPGKYWEPFAFPVGCSGTEEEEGLRIGR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 192	167	Group XIIA secretory phospholipase A2		PRO_0000022771
Sites
	Active site	113	1	By similarity
	Active site	128	1	By similarity
	Metal binding	91	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	93	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	95	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	114	1	Calcium By similarity
Natural variations
	Alternative sequence	1 – 73	73	MVTPR…KCSDG → MKDYHSGPGKYWEPFAFPVG CSGTEEEEGLRIGR in isoform 2.		VSP_004509
Experimental info
	Sequence conflict	11	1	S → G in AAG23336. Ref.1
	Sequence conflict	173	1	P → H in BAB26094. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 670ACE8F6AB6FCA2	FASTA	192	21,319
	10 20 30 40 50 60 MVTPRPAPAR SPALLLLLLL ATARGQEQDQ TTDWRATLKT IRNGIHKIDT YLNAALDLLG 70 80 90 100 110 120 GEDGLCQYKC SDGSKPVPRY GYKPSPPNGC GSPLFGVHLN IGIPSLTKCC NQHDRCYETC 130 140 150 160 170 180 GKSKNDCDEE FQYCLSKICR DVQKTLGLSQ NVQACETTVE LLFDSVIHLG CKPYLDSQRA 190 ACWCRYEEKT DL
	Isoform 2 [UniParc]. Checksum: C9021DB502233080	FASTA	153	17,199
	10 20 30 40 50 60 MKDYHSGPGK YWEPFAFPVG CSGTEEEEGL RIGRSKPVPR YGYKPSPPNG CGSPLFGVHL 70 80 90 100 110 120 NIGIPSLTKC CNQHDRCYET CGKSKNDCDE EFQYCLSKIC RDVQKTLGLS QNVQACETTV 130 140 150 ELLFDSVIHL GCKPYLDSQR AACWCRYEEK TDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel group of phospholipase A2s preferentially expressed in type 2 helper T cells." Ho I.C., Arm J.P., Bingham C.O. III, Choi A., Austen K.F., Glimcher L.H. J. Biol. Chem. 276:18321-18326(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AKR.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum, Embryo and Tongue.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY007381 mRNA. Translation: AAG23336.1. AY007382 mRNA. Translation: AAG23337.1. AK003183 mRNA. No translation available. AK009133 mRNA. Translation: BAB26094.1. AK010011 mRNA. Translation: BAB26641.1. AK010174 mRNA. Translation: BAB26747.1. AK163693 mRNA. Translation: BAE37460.1. BC051117 mRNA. Translation: AAH51117.1.
IPI	IPI00110259. IPI00230716.
RefSeq	NP_075685.2. NM_023196.3. NP_904359.1. NM_183423.2.
UniGene	Mm.151951.
3D structure databases
ProteinModelPortal	Q9EPR2.
ModBase	Search...
PTM databases
PhosphoSite	Q9EPR2.
Proteomic databases
PaxDb	Q9EPR2.
PRIDE	Q9EPR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029629; ENSMUSP00000029629; ENSMUSG00000027999. ENSMUST00000061165; ENSMUSP00000053651; ENSMUSG00000027999.
GeneID	66350.
KEGG	mmu:66350.
UCSC	uc008rip.2. mouse. uc012cxt.1. mouse.
Organism-specific databases
CTD	81579.
MGI	MGI:1913600. Pla2g12a.
Phylogenomic databases
eggNOG	NOG68062.
GeneTree	ENSGT00390000008798.
HOGENOM	HOG000247019.
HOVERGEN	HBG053577.
InParanoid	Q9EPR2.
KO	K01047.
OMA	MHLGCKP.
OrthoDB	EOG4MPHR9.
Gene expression databases
Bgee	Q9EPR2.
CleanEx	MM_PLA2G12A.
Genevestigator	Q9EPR2.
GermOnline	ENSMUSG00000027999. Mus musculus.
Family and domain databases
Gene3D	1.20.90.10. 1 hit.
InterPro	IPR013090. PLipase_A2_AS. IPR016090. PLipase_A2_dom. IPR010711. PLipase_A2_secretory_G12. [Graphical view]
PANTHER	PTHR12824. PTHR12824. 1 hit.
Pfam	PF06951. PLA2G12. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. False negative. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	321399.
SOURCE	Search...

Entry information

Entry name

PG12A_MOUSE

Accession

Primary (citable) accession number: Q9EPR2
Secondary accession number(s): Q3TQC4 , Q9CQR3, Q9CTL1, Q9D7L3, Q9EPR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	April 16, 2002
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program