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Protein

Neurobeachin

Gene

Nbea

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins. May have a role in membrane trafficking.1 Publication1 Publication

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB
  • protein kinase binding Source: MGI

GO - Biological processi

  • post-Golgi vesicle-mediated transport Source: UniProtKB
  • protein localization Source: MGI
  • protein targeting Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Neurobeachin
Alternative name(s):
Lysosomal-trafficking regulator 2
Gene namesi
Name:Nbea
Synonyms:Lyst2
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1347075. Nbea.

Subcellular locationi

  • Membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Endomembrane system 1 Publication; Peripheral membrane protein 1 Publication
  • Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Associated with pleomorphic tubulovesicular endomembranes near the trans sides of Golgi stacks and throughout the cell bodies and cell processes. Concentrated at the postsynaptic plasma membrane of a subpopulation of synapses.

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • endomembrane system Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510901 – 2936NeurobeachinAdd BLAST2936

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1001PhosphoserineCombined sources1
Modified residuei1004PhosphoserineCombined sources1
Modified residuei1519PhosphoserineCombined sources1
Modified residuei1704PhosphoserineCombined sources1
Modified residuei1707PhosphoserineCombined sources1
Modified residuei2128PhosphoserineCombined sources1
Modified residuei2565PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9EPN1.
PeptideAtlasiQ9EPN1.
PRIDEiQ9EPN1.

PTM databases

iPTMnetiQ9EPN1.
PhosphoSitePlusiQ9EPN1.

Expressioni

Tissue specificityi

Forebrain, brainstem and cerebellum.1 Publication

Developmental stagei

Highly expressed in neonatal brain, levels decline in adults.1 Publication

Gene expression databases

BgeeiENSMUSG00000027799.
CleanExiMM_NBEA.
GenevisibleiQ9EPN1. MM.

Interactioni

Subunit structurei

Interacts with RII subunit of PKA.1 Publication

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB
  • protein kinase binding Source: MGI

Protein-protein interaction databases

IntActiQ9EPN1. 3 interactors.
MINTiMINT-4103083.
STRINGi10090.ENSMUSP00000029374.

Structurei

3D structure databases

ProteinModelPortaliQ9EPN1.
SMRiQ9EPN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1316 – 1358WD 1CuratedAdd BLAST43
Domaini2137 – 2245BEACH-type PHPROSITE-ProRule annotationAdd BLAST109
Domaini2264 – 2553BEACHPROSITE-ProRule annotationCuratedAdd BLAST290
Repeati2708 – 2751WD 2CuratedAdd BLAST44
Repeati2768 – 2808WD 3CuratedAdd BLAST41
Repeati2850 – 2889WD 4CuratedAdd BLAST40
Repeati2892 – 2931WD 5CuratedAdd BLAST40

Domaini

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.1 Publication

Sequence similaritiesi

Belongs to the WD repeat neurobeachin family.Curated
Contains 1 BEACH domain.PROSITE-ProRule annotationCurated
Contains 1 BEACH-type PH domain.PROSITE-ProRule annotation
Contains 5 WD repeats.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1786. Eukaryota.
ENOG410XNQC. LUCA.
GeneTreeiENSGT00760000119083.
HOGENOMiHOG000007936.
HOVERGENiHBG012582.
InParanoidiQ9EPN1.
OMAiEANHYGP.
OrthoDBiEOG091G024D.
PhylomeDBiQ9EPN1.
TreeFamiTF313490.

Family and domain databases

CDDicd06071. Beach. 1 hit.
Gene3Di1.10.1540.10. 1 hit.
2.130.10.10. 1 hit.
2.30.29.40. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000409. BEACH_dom.
IPR013320. ConA-like_dom.
IPR010508. DUF1088.
IPR031570. DUF4704.
IPR023362. PH-BEACH_dom.
IPR011993. PH_dom-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02138. Beach. 1 hit.
PF06469. DUF1088. 1 hit.
PF15787. DUF4704. 1 hit.
PF14844. PH_BEACH. 1 hit.
[Graphical view]
SMARTiSM01026. Beach. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF50978. SSF50978. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEiPS50197. BEACH. 1 hit.
PS51783. PH_BEACH. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9EPN1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASDKPGPGL EPQPVALLAV GAGGGAGGGG AMGEPRGAAG SGPVVLPAGM
60 70 80 90 100
INPSVPIRNI RMKFAVLIGL IQVGEVSNRD IVETVLNLLV GGEFDLEMNF
110 120 130 140 150
IIQDAESITC MTELLEHCDV TCQAEIWSMF TAILRKSVRN LQTSTEVGLI
160 170 180 190 200
EQVLLKMSAV DDMIADLLVD MLGVLASYSI TVKELKLLFS MLRGESGIWP
210 220 230 240 250
RHAVKLLSVL NQMPQRHGPD TFFNFPGCSA AAIALPPIAK WPYQNGFTLN
260 270 280 290 300
TWFRMDPLNN INVDKDKPYL YCFRTSKGVG YSAHFVGNCL IVTSLKSKGK
310 320 330 340 350
GFQHCVKYDF QPRKWYMISI VHIYNRWRNS EIRCYVNGQL VSYGDMAWHV
360 370 380 390 400
NTNDSYDKCF LGSSETADAN RVFCGQLGAV YVFSEALNPA QIFAVHQLGP
410 420 430 440 450
GYKSTFKFKS ESDIHLAEHH KQVLYDGKLA SSIAFSYNAK ATDAQLCLES
460 470 480 490 500
SPKENASIFV HSPHALMLQD VKAIVTHSIH SAIHSIGGIQ VLFPLFAQLD
510 520 530 540 550
NRQLNDSQVE TTVCATLLAF LVELLKSSVA MQEQMLGGKG FLVIGYLLEK
560 570 580 590 600
SSRVHITRAV LEQFLSFAKY LDGLSHGAPL LKQLCDHILF NPAIWIHTPA
610 620 630 640 650
KVQLSLYTYL SAEFIGTATI YTTIRRVGTV LQLMHTLKYY YWVINPADSS
660 670 680 690 700
GIAPKGLDGP RPSQKEIISL RAFMLLFLKQ LILKDRGVKE DELQSILNYL
710 720 730 740 750
LTMHEDENIH DVLQLLVALM SEHPASMIPA FDQRNGIRVI YKLLASKSES
760 770 780 790 800
IWVQALKVLG YFLKHLGHKR KVEIMHTHSL FTLLGERLML HTNTVTVTTY
810 820 830 840 850
NTLYEILTEQ VCTQVVHKPH PEPDSTVKIQ NPMILKVVAT LLKNSTPSAE
860 870 880 890 900
LMEVRRLFLS DMIKLFSNSR ENRRCLLQCS VWQDWMFSLG YINPKSSEEQ
910 920 930 940 950
KITEMVYNIF RILLYHAIKY EWGGWRVWVD TLSIAHSKVT YEAHKEYLAK
960 970 980 990 1000
MYEEYQRQEE ENIKKGKKGN VSTISGLSSQ TAGAKGGMEI REIEDLSQSQ
1010 1020 1030 1040 1050
SPESETDYPV STDTRDLLMS TKVSDDILGS SDRPGSGVHV EVHDLLVDIK
1060 1070 1080 1090 1100
AEKVEATEVK LDDMDLSPET LVGGENGALV EVESLLDNVY SAAVEKLQNN
1110 1120 1130 1140 1150
VHGSVGIIKK NEEKDNGPLI TLADEKEELP NSSTPFLFDK IPRQEEKLLP
1160 1170 1180 1190 1200
ELSSNHIIPN IQDTQVHLGV SDDLGLLAHM TASVELTCTS SIMEEKDFRI
1210 1220 1230 1240 1250
HTTSDGVSSV SERELASSTK GLDYAEMTAT TLETESSNSK AVPNVDAGSI
1260 1270 1280 1290 1300
ISDTERSDDG KESGKEIRKI QTTATTQAVQ GRSSTQQDRD LRVDLGFRGM
1310 1320 1330 1340 1350
PMTEEQRRQF SPGPRTTMFR IPEFKWSPMH QRLLTDLLFA LETDVHVWRS
1360 1370 1380 1390 1400
HSTKSVMDFV NSNENIIFVH NTIHLISQMV DNIIIACGGI LPLLSAATSP
1410 1420 1430 1440 1450
TGSKTELENI EVTQGMSAET AVTFLSRLMA MVDVLVFASS LNFSEIEAEK
1460 1470 1480 1490 1500
NMSSGGLMRQ CLRLVCCVAV RNCLECRQRQ RDRGSKSSHG SSKPQEAPHS
1510 1520 1530 1540 1550
VTAASASKTP LENVPGNLSP IKDPDRLLQD VDINRLRAVV FRDVDDSKQA
1560 1570 1580 1590 1600
QFLALAVVYF ISVLMVSKYR DILEPQRETA RTGSQPGRNI RQEINSPTST
1610 1620 1630 1640 1650
VVVIPSIPHP SLNHGLLAKL MPEQSFAHSF YKETPATFPD TVKEKETPTP
1660 1670 1680 1690 1700
GEDIQLESSV PHTDSGMGEE QVASILDGAE LEPAAGPDAM SELLSTLSSE
1710 1720 1730 1740 1750
VKKSQESLTE HPSEMLKPAP SISSISQTKG INVKEILKSL VAAPVEIAEC
1760 1770 1780 1790 1800
GPEPIPYPDP ALKREAHAIL PMQFHSFDRS VVVPVKKPPP GSLAVTTVGA
1810 1820 1830 1840 1850
TAAGSGLPTG STSSIFAAPG ATPKSMINTT GAVDSGSSSS SSSSSFVNGA
1860 1870 1880 1890 1900
TSKNLPAVQT VAPMPEDSAE NMSITAKLER ALEKVAPLLR EIFVDFAPFL
1910 1920 1930 1940 1950
SRTLLGSHGQ ELLIEGLVCM KSSTSVVELV MLLCSQEWQN SIQKNAGLAF
1960 1970 1980 1990 2000
IELINEGRLL CHAMKDHIVR VANEAEFILN RQRAEDVHKH AEFESQCAQY
2010 2020 2030 2040 2050
AADRREEEKM CDHLISAAKH RDHVTANQLK QKILNILTNK HGAWGAVSHS
2060 2070 2080 2090 2100
QLHDFWRLDY WEDDLRRRRR FVRNAFGSTH AEALLKSAVE YGTEEDVVKS
2110 2120 2130 2140 2150
KKAFRSQAIV NQNSETELML EGDDDAVSLL QEKEIDNLAG PVVLSTPAQL
2160 2170 2180 2190 2200
IAPVVVAKGT LSITTTEIYF EVDEDDAAFK KIDTKVLAYT EGLHGKWMFS
2210 2220 2230 2240 2250
EIRAVFSRRY LLQNTALEVF MANRTSVMFN FPDQATVKKV VYSLPRVGVG
2260 2270 2280 2290 2300
TSYGLPQARR ISLATPRQLY KSSNMTQRWQ RREISNFEYL MFLNTIAGRT
2310 2320 2330 2340 2350
YNDLNQYPVF PWVLTNYESE ELDLTLPGNF RDLSKPIGAL NPKRAVFYAE
2360 2370 2380 2390 2400
RYETWEEDQS PPFHYNTHYS TATSALSWLV RIEPFTTFFL NANDGKFDHP
2410 2420 2430 2440 2450
DRTFSSIARS WRTSQRDTSD VKELIPEFYY LPEMFVNSNG YHLGVREDEV
2460 2470 2480 2490 2500
VVNDVDLPPW AKKPEDFVRI NRMALESEFV SCQLHQWIDL IFGYKQRGPE
2510 2520 2530 2540 2550
AVRALNVFHY LTYEGSVNLD SITDPVLREA MEAQIQNFGQ TPSQLLIEPH
2560 2570 2580 2590 2600
PPRSSAMHLC FLPQSPLMFK DQMQQDVIMV LKFPSNSPVT HVAANTLPHL
2610 2620 2630 2640 2650
TIPAVVTVTC SRLFAVNRWH NTVGLRGAPG YSLDQAHHLP IEMDPLIANN
2660 2670 2680 2690 2700
SGVNKRQITD LVDQSIQINA HCFVVTADNR YILICGFWDK SFRVYSTETG
2710 2720 2730 2740 2750
KLTQIVFGHW DVVTCLARSE SYIGGDCYIV SGSRDATLLL WYWSGRHHII
2760 2770 2780 2790 2800
GDNPNSSDYP APRAVLTGHD HEVVCVSVCA ELGLVISGAK EGPCLVHTIT
2810 2820 2830 2840 2850
GDLLRALEGP ENCLFPRLIS VSSEGHCIIY YERGRFSNFS INGKLLAQME
2860 2870 2880 2890 2900
INDSTRAILL SSDGQNLVTG GDNGVVEVWQ ACDFKQLYIY PGCDAGIRAM
2910 2920 2930
DLSHDQRTLI TGMASGSIVA FNIDFNRWHY EHQNRY
Length:2,936
Mass (Da):326,743
Last modified:March 1, 2001 - v1
Checksum:iA6CFD90CA666CEA4
GO
Isoform 2Curated (identifier: Q9EPN1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2197: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:739
Mass (Da):84,006
Checksum:i99FBF86BC953F0F3
GO
Isoform 31 Publication (identifier: Q9EPN1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1601-1632: Missing.

Show »
Length:2,904
Mass (Da):323,202
Checksum:iB41D4ADF71AD6FF4
GO
Isoform 41 Publication (identifier: Q9EPN1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2560-2564: Missing.

Show »
Length:2,931
Mass (Da):326,154
Checksum:iB2E2E8E87E6B2696
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2220 – 2221FM → SR (PubMed:12160729).Curated2
Sequence conflicti2332D → H in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2337I → K in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2375A → P in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2431L → V in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2532E → V in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2539G → A in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2554S → T in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2561F → S in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2802D → N in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2805R → K in AAD41634 (PubMed:12160729).Curated1
Sequence conflicti2920 – 2936AFNID…HQNRY → LLI in AAD41634 (PubMed:12160729).CuratedAdd BLAST17

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0505401 – 2197Missing in isoform 2. 1 PublicationAdd BLAST2197
Alternative sequenceiVSP_0505411601 – 1632Missing in isoform 3. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_0505422560 – 2564Missing in isoform 4. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18276 mRNA. Translation: CAC18811.1.
Y18276 mRNA. Translation: CAC18812.1.
Y18276 mRNA. Translation: CAC18813.1.
AK043125 mRNA. Translation: BAC31466.1.
AF072372 mRNA. Translation: AAD41634.1.
CCDSiCCDS50911.1. [Q9EPN1-1]
RefSeqiNP_085098.1. NM_030595.1. [Q9EPN1-1]
XP_006501586.1. XM_006501523.3. [Q9EPN1-4]
UniGeneiMm.384353.

Genome annotation databases

EnsembliENSMUST00000029374; ENSMUSP00000029374; ENSMUSG00000027799. [Q9EPN1-1]
GeneIDi26422.
KEGGimmu:26422.
UCSCiuc008pgt.2. mouse. [Q9EPN1-2]
uc033htk.1. mouse. [Q9EPN1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18276 mRNA. Translation: CAC18811.1.
Y18276 mRNA. Translation: CAC18812.1.
Y18276 mRNA. Translation: CAC18813.1.
AK043125 mRNA. Translation: BAC31466.1.
AF072372 mRNA. Translation: AAD41634.1.
CCDSiCCDS50911.1. [Q9EPN1-1]
RefSeqiNP_085098.1. NM_030595.1. [Q9EPN1-1]
XP_006501586.1. XM_006501523.3. [Q9EPN1-4]
UniGeneiMm.384353.

3D structure databases

ProteinModelPortaliQ9EPN1.
SMRiQ9EPN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EPN1. 3 interactors.
MINTiMINT-4103083.
STRINGi10090.ENSMUSP00000029374.

PTM databases

iPTMnetiQ9EPN1.
PhosphoSitePlusiQ9EPN1.

Proteomic databases

PaxDbiQ9EPN1.
PeptideAtlasiQ9EPN1.
PRIDEiQ9EPN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029374; ENSMUSP00000029374; ENSMUSG00000027799. [Q9EPN1-1]
GeneIDi26422.
KEGGimmu:26422.
UCSCiuc008pgt.2. mouse. [Q9EPN1-2]
uc033htk.1. mouse. [Q9EPN1-1]

Organism-specific databases

CTDi26960.
MGIiMGI:1347075. Nbea.

Phylogenomic databases

eggNOGiKOG1786. Eukaryota.
ENOG410XNQC. LUCA.
GeneTreeiENSGT00760000119083.
HOGENOMiHOG000007936.
HOVERGENiHBG012582.
InParanoidiQ9EPN1.
OMAiEANHYGP.
OrthoDBiEOG091G024D.
PhylomeDBiQ9EPN1.
TreeFamiTF313490.

Miscellaneous databases

ChiTaRSiNbea. mouse.
PROiQ9EPN1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027799.
CleanExiMM_NBEA.
GenevisibleiQ9EPN1. MM.

Family and domain databases

CDDicd06071. Beach. 1 hit.
Gene3Di1.10.1540.10. 1 hit.
2.130.10.10. 1 hit.
2.30.29.40. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000409. BEACH_dom.
IPR013320. ConA-like_dom.
IPR010508. DUF1088.
IPR031570. DUF4704.
IPR023362. PH-BEACH_dom.
IPR011993. PH_dom-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02138. Beach. 1 hit.
PF06469. DUF1088. 1 hit.
PF15787. DUF4704. 1 hit.
PF14844. PH_BEACH. 1 hit.
[Graphical view]
SMARTiSM01026. Beach. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF50978. SSF50978. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEiPS50197. BEACH. 1 hit.
PS51783. PH_BEACH. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNBEA_MOUSE
AccessioniPrimary (citable) accession number: Q9EPN1
Secondary accession number(s): Q8C931
, Q9EPM9, Q9EPN0, Q9WVM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.