Q9EPM5 (SYNCI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syncoilin

Alternative name(s):
Syncoilin intermediate filament 1
Syncoilin-1

Gene names

Name:	Sync
Synonyms:	Sync1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with DES and DTNA. Ref.1 Ref.5
Subcellular location	Cytoplasm › perinuclear region. Note: In skeletal muscle, colocalizes with DES and DTNA, and is localized at the myotendinous and neuromuscular junctions, sarcolemma and Z-lines. In myotubes, detected in a punctate cytoplasmic pattern. Ref.1 Ref.5 Ref.6
Tissue specificity	Detected strongly in skeletal muscle and heart and weakly in lung (at protein level). Highly expressed in skeletal muscle and lung and weakly in lung and testis. Ref.1
Induction	Up-regulated in dystrophic muscle (at protein level). Ref.1 Ref.6
Sequence similarities	Belongs to the intermediate filament family.

Ontologies

Keywords
Cellular component	Cytoplasm Intermediate filament
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intermediate filament-based process Inferred from direct assay PubMed 11330864. Source: MGI
Cellular_component	Z disc Inferred from direct assay Ref.5. Source: MGI cytosol Inferred from direct assay Ref.5. Source: MGI intermediate filament Inferred from sequence or structural similarity Ref.1. Source: MGI neuromuscular junction Inferred from direct assay PubMed 11330864. Source: MGI perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell sarcolemma Inferred from direct assay Ref.5. Source: MGI
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q9EPM5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: Q9EPM5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     443-453: AMLPKSLEQAD → SVFSASQVSQI
     454-470: Missing.

Note: No experimental confirmation available.

Isoform 3 Ref.2 (identifier: Q9EPM5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Syncoilin		PRO_0000306181
Regions
	Region	1 – 148	148	Head
	Region	149 – 157	9	Rod
	Region	158 – 192	35	Coil 1A
	Region	193 – 219	27	Linker 1
	Region	220 – 297	78	Coil 1b
	Region	298 – 337	40	Linker 2
	Region	338 – 445	108	Coil 2
	Region	446 – 470	25	Tail
Amino acid modifications
	Modified residue	314	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 103	103	Missing in isoform 3. Ref.2		VSP_052550
	Alternative sequence	443 – 453	11	AMLPKSLEQAD → SVFSASQVSQI in isoform 2. Ref.2		VSP_052551
	Alternative sequence	454 – 470	17	Missing in isoform 2. Ref.2		VSP_052552
Experimental info
	Sequence conflict	23	1	A → E in AAI06856. Ref.4
	Sequence conflict	79	1	L → Q in AAI06856. Ref.4
	Sequence conflict	377	1	Q → K in BAC38387. Ref.2
	Sequence conflict	432	1	M → T in AAI06856. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3D3B6026B099B229	FASTA	470	53,630
	10 20 30 40 50 60 MASPEPLRGG DGARASREPH TEASFPLQES ESPKEAKTFN PEATLSLEGT VNLEDILYLG 70 80 90 100 110 120 ASGDFEESFY EEEYEKPALT LFIDESRQPD EALGLEEPVR PEEMLSVEES VTPDEVQISE 130 140 150 160 170 180 QPVEPAKSPT ACEGEMVATE GSLPAQPIPN TEEDPLSVED LERLEARFQQ CVQAVSQLEE 190 200 210 220 230 240 ERDQLIHELV LLREPALQEV QQVHQDILAA YKLHAQAELE RDGLREEIRT VKQKLFKVTK 250 260 270 280 290 300 ECVAYQYQLE CRQQDVAQFA DCREALTTRA AQLSEELTQL RDACQKQKEQ LQQQLEAPPT 310 320 330 340 350 360 QSDGHFLQES RRLSTQFENL MAESRQGLEE EYEPQLLRLL ERKEAGTKAL QDTQAEIQEM 370 380 390 400 410 420 REALRPLEAE ARQLQLQNRN LEDQITLVRQ KRDEEVQQYR EQLEEMEERQ RQLRSGVQVQ 430 440 450 460 470 QQKNKEMERL RMSLAEELST YKAMLPKSLE QADAPTSQAG GVEAQSPGTV
	Isoform 2 [UniParc]. Checksum: 9F7360528BE73B54	FASTA	453	52,041
	10 20 30 40 50 60 MASPEPLRGG DGARASREPH TEASFPLQES ESPKEAKTFN PEATLSLEGT VNLEDILYLG 70 80 90 100 110 120 ASGDFEESFY EEEYEKPALT LFIDESRQPD EALGLEEPVR PEEMLSVEES VTPDEVQISE 130 140 150 160 170 180 QPVEPAKSPT ACEGEMVATE GSLPAQPIPN TEEDPLSVED LERLEARFQQ CVQAVSQLEE 190 200 210 220 230 240 ERDQLIHELV LLREPALQEV QQVHQDILAA YKLHAQAELE RDGLREEIRT VKQKLFKVTK 250 260 270 280 290 300 ECVAYQYQLE CRQQDVAQFA DCREALTTRA AQLSEELTQL RDACQKQKEQ LQQQLEAPPT 310 320 330 340 350 360 QSDGHFLQES RRLSTQFENL MAESRQGLEE EYEPQLLRLL ERKEAGTKAL QDTQAEIQEM 370 380 390 400 410 420 REALRPLEAE ARQLQLQNRN LEDQITLVRQ KRDEEVQQYR EQLEEMEERQ RQLRSGVQVQ 430 440 450 QQKNKEMERL RMSLAEELST YKSVFSASQV SQI
	Isoform 3 [UniParc]. Checksum: 4C0EE9F8994C9F1C	FASTA	367	42,230
	10 20 30 40 50 60 MLSVEESVTP DEVQISEQPV EPAKSPTACE GEMVATEGSL PAQPIPNTEE DPLSVEDLER 70 80 90 100 110 120 LEARFQQCVQ AVSQLEEERD QLIHELVLLR EPALQEVQQV HQDILAAYKL HAQAELERDG 130 140 150 160 170 180 LREEIRTVKQ KLFKVTKECV AYQYQLECRQ QDVAQFADCR EALTTRAAQL SEELTQLRDA 190 200 210 220 230 240 CQKQKEQLQQ QLEAPPTQSD GHFLQESRRL STQFENLMAE SRQGLEEEYE PQLLRLLERK 250 260 270 280 290 300 EAGTKALQDT QAEIQEMREA LRPLEAEARQ LQLQNRNLED QITLVRQKRD EEVQQYREQL 310 320 330 340 350 360 EEMEERQRQL RSGVQVQQQK NKEMERLRMS LAEELSTYKA MLPKSLEQAD APTSQAGGVE AQSPGTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Syncoilin, a novel member of the intermediate filament superfamily that interacts with alpha-dystrobrevin in skeletal muscle." Newey S.E., Howman E.V., Ponting C.P., Benson M.A., Nawrotzki R., Loh N.Y., Davies K.E., Blake D.J. J. Biol. Chem. 276:6645-6655(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH DTNA, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J. Tissue: Blastocyst, Embryo and Embryonic head.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Association of syncoilin and desmin: linking intermediate filament proteins to the dystrophin-associated protein complex." Poon E., Howman E.V., Newey S.E., Davies K.E. J. Biol. Chem. 277:3433-3439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DTNA, SUBCELLULAR LOCATION.
[6]	"Intermediate filament-like protein syncoilin in normal and myopathic striated muscle." McCullagh K.J.A., Edwards B., Poon E., Lovering R.M., Paulin D., Davies K.E. Neuromuscul. Disord. 17:970-979(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ251641 mRNA. Translation: CAC17787.1. AK004279 mRNA. Translation: BAB23246.1. AK081983 mRNA. Translation: BAC38387.1. AK160756 mRNA. Translation: BAE35992.1. AK166916 mRNA. Translation: BAE39114.1. AL607123 Genomic DNA. Translation: CAM22957.1. BC106855 mRNA. Translation: AAI06856.1.
IPI	IPI00459104. IPI00867826. IPI00867843.
RefSeq	NP_075974.3. NM_023485.3.
UniGene	Mm.132243.
3D structure databases
ProteinModelPortal	Q9EPM5.
SMR	Q9EPM5. Positions 158-282, 306-449.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-148967.
PTM databases
PhosphoSite	Q9EPM5.
Proteomic databases
PaxDb	Q9EPM5.
PRIDE	Q9EPM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102599; ENSMUSP00000099659; ENSMUSG00000001333.
GeneID	68828.
KEGG	mmu:68828.
UCSC	uc008uwq.1. mouse. uc008uws.1. mouse.
Organism-specific databases
CTD	81493.
MGI	MGI:1916078. Sync.
Phylogenomic databases
eggNOG	NOG79532.
GeneTree	ENSGT00390000018108.
HOGENOM	HOG000010275.
HOVERGEN	HBG108528.
InParanoid	Q9EPM5.
KO	K10377.
OMA	ECVAYQY.
OrthoDB	EOG47H5RH.
Gene expression databases
ArrayExpress	Q9EPM5.
Bgee	Q9EPM5.
CleanEx	MM_SYNC.
Genevestigator	Q9EPM5.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. [Graphical view]
PANTHER	PTHR23239. PTHR23239. 1 hit.
Pfam	PF00038. Filament. 1 hit. [Graphical view]
PROSITE	PS00226. IF. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	328007.
SOURCE	Search...

Entry information

Entry name

SYNCI_MOUSE

Accession

Primary (citable) accession number: Q9EPM5
Secondary accession number(s): Q3KP79 , Q3TKN1, Q3TUH9, Q8C4J4, Q9CT88

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program