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Q9EPL6 (MMP1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Interstitial collagenase B
EC=3.4.24.7
Alternative name(s):
Matrix metalloproteinase-1b
Short name=MMP-1b
Mcol-B
Gene names
Name:Mmp1b
Synonyms:McolB
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.

Cofactor

Binds 4 calcium ions per subunit By similarity.

Binds 2 zinc ions per subunit By similarity.

Enzyme regulation

Can be activated without removal of the activation peptide By similarity.

Subcellular location

Secreted › extracellular space › extracellular matrix Probable.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similarities

Belongs to the peptidase M10A family.

Contains 4 hemopexin-like domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 9679Activation peptide By similarity
PRO_0000042647
Chain97 – 463367Interstitial collagenase B
PRO_0000042648

Regions

Domain281 – 32343Hemopexin-like 1
Domain325 – 36945Hemopexin-like 2
Domain374 – 42148Hemopexin-like 3
Domain423 – 46341Hemopexin-like 4
Region95 – 273179Metalloprotease By similarity
Motif87 – 948Cysteine switch By similarity

Sites

Active site2161 By similarity
Metal binding891Zinc 2; in inhibited form By similarity
Metal binding1551Calcium 2 By similarity
Metal binding1651Zinc 1 By similarity
Metal binding1671Zinc 1 By similarity
Metal binding1721Calcium 3 By similarity
Metal binding1731Calcium 3; via carbonyl oxygen By similarity
Metal binding1801Zinc 1 By similarity
Metal binding1871Calcium 2; via carbonyl oxygen By similarity
Metal binding1891Calcium 2; via carbonyl oxygen By similarity
Metal binding1931Zinc 1 By similarity
Metal binding1951Calcium 3 By similarity
Metal binding2151Zinc 2; catalytic By similarity
Metal binding2191Zinc 2; catalytic By similarity
Metal binding2251Zinc 2; catalytic By similarity
Metal binding2821Calcium 4; via carbonyl oxygen By similarity
Metal binding3751Calcium 4; via carbonyl oxygen By similarity
Metal binding4241Calcium 4; via carbonyl oxygen By similarity

Amino acid modifications

Glycosylation3701N-linked (GlcNAc...) Potential
Disulfide bond275 ↔ 463 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9EPL6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 46013AB9D106C3F1

FASTA46353,492
        10         20         30         40         50         60 
MPSLPLLLRL WAASSYSFPV IQDGLQKNVK TVWKYLENYY NLGKNMQAKN VNGKEVMAEK 

        70         80         90        100        110        120 
LRQMQQLFGL KVTGNSDPET LRAMKKPRCG VPDVAPYAIT HNNPRWTKTH LTYSILNYTP 

       130        140        150        160        170        180 
YLSKAVVEDA IARAFRVWSD VTPLTFQRVF EEEGDIVLSF HRGDHGDLYT FDGSKYHFAH 

       190        200        210        220        230        240 
AFLPGLGLGG NVHYDLDQKW TDNNEDFNLF YVTAHELGHS LGLSHSNDEE ALMFPSYTWS 

       250        260        270        280        290        300 
NKDFVLNQDD INRIQALYGP SPNPIQLTDA TLDPCNSGLT FDAIITYRGE VIFFKDRFYI 

       310        320        330        340        350        360 
RVISFLPEPL IDVIDLTWPN LPGKFDAAYE VSGVDELRFF KGSKVWAVQE QNVLEGFPMD 

       370        380        390        400        410        420 
IQSFFGFPSN VTNIDAAVCE EETGKTYFFV DHMYWRYDEN TRSMDPGYPR LIAEDFPGID 

       430        440        450        460 
YKVDDVIQKE DNFYFFHQSI QYRFNLKTRR IDDSSDINTW FNC

« Hide

References

[1]"Identification and enzymatic characterization of two diverging murine counterparts of human interstitial collagenase (MMP-1) expressed at sites of embryo implantation."
Balbin M., Fueyo A., Knauper V., Lopez J.M., Alvarez J., Sanchez L.M., Quesada V., Bordallo J., Murphy G., Lopez-Otin C.
J. Biol. Chem. 276:10253-10262(2001) [PubMed: 11113146] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ278461 mRNA. Translation: CAC18879.1.
IPIIPI00110076.
UniGeneMm.379271.

3D structure databases

ProteinModelPortalQ9EPL6.
SMRQ9EPL6. Positions 29-462.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9EPL6.

Protein family/group databases

MEROPSM10.034.

Proteomic databases

PRIDEQ9EPL6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCuc009ocn.1. mouse.

Organism-specific databases

MGIMGI:1933847. Mmp1b.

Phylogenomic databases

GeneTreeENSGT00580000081219.
HOGENOMHBG747685.
HOVERGENHBG052484.
InParanoidQ9EPL6.
OrthoDBEOG4X97GX.

Gene expression databases

ArrayExpressQ9EPL6.
BgeeQ9EPL6.
GenevestigatorQ9EPL6.
GermOnlineENSMUSG00000041620. Mus musculus.

Family and domain databases

InterProIPR000585. Hemopexin/matrixin.
IPR018486. Hemopexin/matrixin_CS.
IPR018487. Hemopexin/matrixin_repeat.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR016293. Pept_M10A_matrix_strom.
IPR021190. Pept_M10A_matrixin.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
Gene3DG3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
G3DSA:2.110.10.10. Hemopexin. 1 hit.
PfamPF00045. Hemopexin. 2 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSPR00138. MATRIXIN.
SMARTSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMSSF50923. Hemopexin. 1 hit.
SSF47090. PGBD_like. 1 hit.
PROSITEPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

SOURCESearch...

Entry information

Entry nameMMP1B_MOUSE
AccessionPrimary (citable) accession number: Q9EPL6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: March 1, 2001
Last modified: November 16, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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