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Protein

Interstitial collagenase B

Gene

Mmp1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 4 Ca2+ ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Enzyme regulationi

Can be activated without removal of the activation peptide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi89Zinc 2; in inhibited formBy similarity1
Metal bindingi155Calcium 2By similarity1
Metal bindingi165Zinc 1By similarity1
Metal bindingi167Zinc 1By similarity1
Metal bindingi172Calcium 3By similarity1
Metal bindingi173Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi180Zinc 1By similarity1
Metal bindingi187Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi189Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi193Zinc 1By similarity1
Metal bindingi195Calcium 3By similarity1
Metal bindingi215Zinc 2; catalyticBy similarity1
Active sitei216PROSITE-ProRule annotation1
Metal bindingi219Zinc 2; catalyticBy similarity1
Metal bindingi225Zinc 2; catalyticBy similarity1
Metal bindingi282Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi375Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi424Calcium 4; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.034.

Names & Taxonomyi

Protein namesi
Recommended name:
Interstitial collagenase B (EC:3.4.24.7)
Alternative name(s):
Matrix metalloproteinase-1b
Short name:
MMP-1b
Mcol-B
Gene namesi
Name:Mmp1b
Synonyms:McolB
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1933847. Mmp1b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000004264718 – 96Activation peptideBy similarityAdd BLAST79
ChainiPRO_000004264897 – 463Interstitial collagenase BAdd BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi275 ↔ 463By similarity
Glycosylationi370N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9EPL6.
PRIDEiQ9EPL6.

PTM databases

PhosphoSitePlusiQ9EPL6.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047261.

Structurei

3D structure databases

ProteinModelPortaliQ9EPL6.
SMRiQ9EPL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati278 – 321Hemopexin 1Add BLAST44
Repeati322 – 368Hemopexin 2Add BLAST47
Repeati371 – 419Hemopexin 3Add BLAST49
Repeati420 – 463Hemopexin 4Add BLAST44

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 273MetalloproteaseBy similarityAdd BLAST179

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi87 – 94Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiQ9EPL6.
PhylomeDBiQ9EPL6.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 2 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EPL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLPLLLRL WAASSYSFPV IQDGLQKNVK TVWKYLENYY NLGKNMQAKN
60 70 80 90 100
VNGKEVMAEK LRQMQQLFGL KVTGNSDPET LRAMKKPRCG VPDVAPYAIT
110 120 130 140 150
HNNPRWTKTH LTYSILNYTP YLSKAVVEDA IARAFRVWSD VTPLTFQRVF
160 170 180 190 200
EEEGDIVLSF HRGDHGDLYT FDGSKYHFAH AFLPGLGLGG NVHYDLDQKW
210 220 230 240 250
TDNNEDFNLF YVTAHELGHS LGLSHSNDEE ALMFPSYTWS NKDFVLNQDD
260 270 280 290 300
INRIQALYGP SPNPIQLTDA TLDPCNSGLT FDAIITYRGE VIFFKDRFYI
310 320 330 340 350
RVISFLPEPL IDVIDLTWPN LPGKFDAAYE VSGVDELRFF KGSKVWAVQE
360 370 380 390 400
QNVLEGFPMD IQSFFGFPSN VTNIDAAVCE EETGKTYFFV DHMYWRYDEN
410 420 430 440 450
TRSMDPGYPR LIAEDFPGID YKVDDVIQKE DNFYFFHQSI QYRFNLKTRR
460
IDDSSDINTW FNC
Length:463
Mass (Da):53,492
Last modified:March 1, 2001 - v1
Checksum:i46013AB9D106C3F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278461 mRNA. Translation: CAC18879.1.
CCDSiCCDS22805.1.
UniGeneiMm.379271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278461 mRNA. Translation: CAC18879.1.
CCDSiCCDS22805.1.
UniGeneiMm.379271.

3D structure databases

ProteinModelPortaliQ9EPL6.
SMRiQ9EPL6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047261.

Protein family/group databases

MEROPSiM10.034.

PTM databases

PhosphoSitePlusiQ9EPL6.

Proteomic databases

PaxDbiQ9EPL6.
PRIDEiQ9EPL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1933847. Mmp1b.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiQ9EPL6.
PhylomeDBiQ9EPL6.

Miscellaneous databases

PROiQ9EPL6.
SOURCEiSearch...

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 2 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP1B_MOUSE
AccessioniPrimary (citable) accession number: Q9EPL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.