Q9EPL6 (MMP1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 71.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Interstitial collagenase B EC=3.4.24.7 Alternative name(s): Matrix metalloproteinase-1b Short name=MMP-1b Mcol-B | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 463 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Catalytic activity | Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue. |
| Cofactor | Binds 4 calcium ions per subunit By similarity. Binds 2 zinc ions per subunit By similarity. |
| Enzyme regulation | Can be activated without removal of the activation peptide By similarity. |
| Subcellular location | Secreted › extracellular space › extracellular matrix Probable. |
| Domain | The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. |
| Sequence similarities | Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Collagen degradation |
| Cellular component | Extracellular matrix Secreted |
| Domain | Repeat Signal |
| Ligand | Calcium Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| PTM | Autocatalytic cleavage Disulfide bond Glycoprotein Zymogen |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: InterPro metalloendopeptidase activityInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||
Molecule processing | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 17 | 17 | Potential | |||||||
| Propeptide | 18 – 96 | 79 | Activation peptide By similarity | PRO_0000042647 | ||||||
| Chain | 97 – 463 | 367 | Interstitial collagenase B | PRO_0000042648 | ||||||
Regions | ||||||||||
| Domain | 281 – 323 | 43 | Hemopexin-like 1 | |||||||
| Domain | 325 – 369 | 45 | Hemopexin-like 2 | |||||||
| Domain | 374 – 421 | 48 | Hemopexin-like 3 | |||||||
| Domain | 423 – 463 | 41 | Hemopexin-like 4 | |||||||
| Region | 95 – 273 | 179 | Metalloprotease By similarity | |||||||
| Motif | 87 – 94 | 8 | Cysteine switch By similarity | |||||||
Sites | ||||||||||
| Active site | 216 | 1 | By similarity | |||||||
| Metal binding | 89 | 1 | Zinc 2; in inhibited form By similarity | |||||||
| Metal binding | 155 | 1 | Calcium 2 By similarity | |||||||
| Metal binding | 165 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 167 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 172 | 1 | Calcium 3 By similarity | |||||||
| Metal binding | 173 | 1 | Calcium 3; via carbonyl oxygen By similarity | |||||||
| Metal binding | 180 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 187 | 1 | Calcium 2; via carbonyl oxygen By similarity | |||||||
| Metal binding | 189 | 1 | Calcium 2; via carbonyl oxygen By similarity | |||||||
| Metal binding | 193 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 195 | 1 | Calcium 3 By similarity | |||||||
| Metal binding | 215 | 1 | Zinc 2; catalytic By similarity | |||||||
| Metal binding | 219 | 1 | Zinc 2; catalytic By similarity | |||||||
| Metal binding | 225 | 1 | Zinc 2; catalytic By similarity | |||||||
| Metal binding | 282 | 1 | Calcium 4; via carbonyl oxygen By similarity | |||||||
| Metal binding | 375 | 1 | Calcium 4; via carbonyl oxygen By similarity | |||||||
| Metal binding | 424 | 1 | Calcium 4; via carbonyl oxygen By similarity | |||||||
Amino acid modifications | ||||||||||
| Glycosylation | 370 | 1 | N-linked (GlcNAc...) Potential | |||||||
| Disulfide bond | 275 ↔ 463 | By similarity | ||||||||
Sequences
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References
| [1] | "Identification and enzymatic characterization of two diverging murine counterparts of human interstitial collagenase (MMP-1) expressed at sites of embryo implantation." Balbin M., Fueyo A., Knauper V., Lopez J.M., Alvarez J., Sanchez L.M., Quesada V., Bordallo J., Murphy G., Lopez-Otin C. J. Biol. Chem. 276:10253-10262(2001) [PubMed: 11113146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ278461 mRNA. Translation: CAC18879.1. |
| IPI | IPI00110076. |
| UniGene | Mm.379271. |
3D structure databases | |
| ProteinModelPortal | Q9EPL6. |
| SMR | Q9EPL6. Positions 29-462. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9EPL6. |
Protein family/group databases | |
| MEROPS | M10.034. |
Proteomic databases | |
| PRIDE | Q9EPL6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| UCSC | uc009ocn.1. mouse. |
Organism-specific databases | |
| MGI | MGI:1933847. Mmp1b. |
Phylogenomic databases | |
| GeneTree | ENSGT00580000081219. |
| HOGENOM | HBG747685. |
| HOVERGEN | HBG052484. |
| InParanoid | Q9EPL6. |
| OrthoDB | EOG4X97GX. |
Gene expression databases | |
| ArrayExpress | Q9EPL6. |
| Bgee | Q9EPL6. |
| Genevestigator | Q9EPL6. |
| GermOnline | ENSMUSG00000041620. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR024079. MetalloPept_cat_dom. IPR001818. Pept_M10_metallopeptidase. IPR016293. Pept_M10A_matrix_strom. IPR021190. Pept_M10A_matrixin. IPR021158. Pept_M10A_Zn_BS. IPR006026. Peptidase_Metallo. IPR002477. Peptidoglycan-bd-like. [Graphical view] |
| Gene3D | G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit. G3DSA:2.110.10.10. Hemopexin. 1 hit. |
| Pfam | PF00045. Hemopexin. 2 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF001191. Peptidase_M10A_matrix. 1 hit. |
| PRINTS | PR00138. MATRIXIN. |
| SMART | SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view] |
| SUPFAM | SSF50923. Hemopexin. 1 hit. SSF47090. PGBD_like. 1 hit. |
| PROSITE | PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| SOURCE | Search... |
Entry information
| Entry name | MMP1B_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9EPL6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with