Reviewed,
UniProtKB/Swiss-Prot Q9EPL5 (MMP1A_MOUSE)
Last modified
October 13, 2009.
Version 62.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Interstitial collagenase A EC=3.4.24.7 Alternative name(s): Mcol-A Matrix metalloproteinase-1a Short name=MMP-1a | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 464 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X By similarity. Able to degrade synthetic peptides and type I and II fibrillar collagen. |
| Catalytic activity | Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue. |
| Cofactor | Binds 4 calcium ions per subunit By similarity. Binds 2 zinc ions per subunit By similarity. |
| Enzyme regulation | Can be activated without removal of the activation peptide By similarity. |
| Subcellular location | Secreted › extracellular space › extracellular matrix Probable. |
| Domain | The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. |
| Sequence similarities | Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Collagen degradation |
| Cellular component | Extracellular matrix Secreted |
| Domain | Repeat Signal |
| Ligand | Calcium Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| PTM | Autocatalytic cleavage Disulfide bond Glycoprotein Zymogen |
| Gene Ontology (GO) | |
| Biological process | collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: InterPro |
| Cellular component | proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activityInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||
Molecule processing | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 17 | 17 | Potential | |||||||
| Propeptide | 18 – 96 | 79 | Activation peptide By similarity | PRO_0000042645 | ||||||
| Chain | 97 – 464 | 368 | Interstitial collagenase A | PRO_0000042646 | ||||||
Regions | ||||||||||
| Domain | 282 – 324 | 43 | Hemopexin-like 1 | |||||||
| Domain | 326 – 370 | 45 | Hemopexin-like 2 | |||||||
| Domain | 375 – 422 | 48 | Hemopexin-like 3 | |||||||
| Domain | 424 – 464 | 41 | Hemopexin-like 4 | |||||||
| Region | 95 – 274 | 180 | Metalloprotease | |||||||
| Motif | 87 – 94 | 8 | Cysteine switch By similarity | |||||||
Sites | ||||||||||
| Active site | 216 | 1 | By similarity | |||||||
| Metal binding | 89 | 1 | Zinc 2; in inhibited form By similarity | |||||||
| Metal binding | 155 | 1 | Calcium 2 By similarity | |||||||
| Metal binding | 165 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 167 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 172 | 1 | Calcium 3 By similarity | |||||||
| Metal binding | 173 | 1 | Calcium 3; via carbonyl oxygen By similarity | |||||||
| Metal binding | 180 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 187 | 1 | Calcium 2; via carbonyl oxygen By similarity | |||||||
| Metal binding | 189 | 1 | Calcium 2; via carbonyl oxygen By similarity | |||||||
| Metal binding | 191 | 1 | Calcium 2 By similarity | |||||||
| Metal binding | 193 | 1 | Zinc 1 By similarity | |||||||
| Metal binding | 195 | 1 | Calcium 3 By similarity | |||||||
| Metal binding | 198 | 1 | Calcium 3 By similarity | |||||||
| Metal binding | 215 | 1 | Zinc 2; catalytic By similarity | |||||||
| Metal binding | 219 | 1 | Zinc 2; catalytic By similarity | |||||||
| Metal binding | 225 | 1 | Zinc 2; catalytic By similarity | |||||||
| Metal binding | 283 | 1 | Calcium 4; via carbonyl oxygen By similarity | |||||||
| Metal binding | 376 | 1 | Calcium 4; via carbonyl oxygen By similarity | |||||||
| Metal binding | 425 | 1 | Calcium 4; via carbonyl oxygen By similarity | |||||||
Amino acid modifications | ||||||||||
| Glycosylation | 202 | 1 | N-linked (GlcNAc...) Potential | |||||||
| Glycosylation | 371 | 1 | N-linked (GlcNAc...) Potential | |||||||
| Disulfide bond | 276 ↔ 464 | By similarity | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification and enzymatic characterization of two diverging murine counterparts of human interstitial collagenase (MMP-1) expressed at sites of embryo implantation." Balbin M., Fueyo A., Knauper V., Lopez J.M., Alvarez J., Sanchez L.M., Quesada V., Bordallo J., Murphy G., Lopez-Otin C. J. Biol. Chem. 276:10253-10262(2001) [PubMed: 11113146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. |
| [3] | "Genomic sequence analysis in the mouse T-complex region." Brathwaite M.E., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129/SvJ. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AJ278462 mRNA. Translation: CAC18880.1. AK049552 mRNA. Translation: BAC33807.1. AY211543 Genomic DNA. Translation: AAO37582.1. BC117756 mRNA. Translation: AAI17757.1. | |
| IPI | IPI00110075. |
| RefSeq | NP_114395.1. |
| UniGene | Mm.156952 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HY7 based on UniProtKB P08254. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9EPL5. |
Protein family/group databases | |
| MEROPS | M10.033. |
Proteomic databases | |
| PRIDE | Q9EPL5. |
Genome annotation databases | |
| Ensembl | ENSMUST00000034492; ENSMUSP00000034492; ENSMUSG00000043089; Mus musculus. [Genome view] |
| GeneID | 83995. |
| KEGG | mmu:83995. |
Organism-specific databases | |
| CTD | 83995. |
| MGI | MGI:1933846. Mmp1a. |
Phylogenomic databases | |
| HOGENOM | Q9EPL5. |
| HOVERGEN | Q9EPL5. |
Enzyme and pathway databases | |
| BRENDA | 3.4.24.7. 244. |
Gene expression databases | |
| ArrayExpress | Q9EPL5. |
| Bgee | Q9EPL5. |
| Genevestigator | Q9EPL5. |
| GermOnline | ENSMUSG00000043089. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001818. Pept_M10A_M12B. IPR016293. Pept_M10A_matrix. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR002477. Peptidoglycan-bd-like. [Graphical view] |
| Gene3D | G3DSA:2.110.10.10. Hemopexin. 1 hit. |
| Pfam | PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF001191. Peptidase_M10A_matrix. 1 hit. |
| PRINTS | PR00138. MATRIXIN. |
| SMART | SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view] |
| PROSITE | PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 350854. |
| SOURCE | Search... |
Entry information
| Entry name | MMP1A_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9EPL5 Secondary accession number(s): Q149J4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


