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Protein

Xylosyltransferase 2

Gene

Xylt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the formation of heparan sulfate and chondroitin sulfate proteoglycans (By similarity). Probably catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes (By similarity).By similarity

Catalytic activityi

Transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxy group of an acceptor protein substrate.

Cofactori

Pathwayi: chondroitin sulfate biosynthesis

This protein is involved in the pathway chondroitin sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway chondroitin sulfate biosynthesis and in Glycan metabolism.

Pathwayi: heparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

GO - Molecular functioni

GO - Biological processi

  • chondroitin sulfate biosynthetic process Source: UniProtKB-UniPathway
  • chondroitin sulfate proteoglycan biosynthetic process Source: UniProtKB
  • glycosaminoglycan biosynthetic process Source: MGI
  • heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
  • heparin biosynthetic process Source: MGI
  • proteoglycan biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00755.
UPA00756.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Xylosyltransferase 2 (EC:2.4.2.26)
Alternative name(s):
Peptide O-xylosyltransferase 2
Xylosyltransferase II
Gene namesi
Name:Xylt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2444797. Xylt2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini37 – 865LumenalSequence analysisAdd BLAST829

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001914071 – 865Xylosyltransferase 2Add BLAST865

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi683N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Contains disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9EPL0.
PaxDbiQ9EPL0.
PRIDEiQ9EPL0.

PTM databases

iPTMnetiQ9EPL0.
PhosphoSitePlusiQ9EPL0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020868.
CleanExiMM_XYLT2.
ExpressionAtlasiQ9EPL0. baseline and differential.
GenevisibleiQ9EPL0. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ9EPL0. 1 interactor.
STRINGi10090.ENSMUSP00000112052.

Structurei

3D structure databases

ProteinModelPortaliQ9EPL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0799. Eukaryota.
ENOG410XQ7M. LUCA.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000070086.
HOVERGENiHBG059443.
InParanoidiQ9EPL0.
KOiK00771.
OMAiVPRHCQL.
OrthoDBiEOG091G020K.
PhylomeDBiQ9EPL0.
TreeFamiTF315534.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
IPR024448. XylT.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
PF12529. Xylo_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVASARVQKL VRRYKLAIAT ALAILLLQGL VVWSFSGLEE DEPGEKGRQR
60 70 80 90 100
KPRPLDPGEG SKDTDSSAGR RGSAGRRHGR WRGRAESPGV PVAKVVRAVT
110 120 130 140 150
SRQRASRRVP PAPPPEAPGR QNLSGAAAGE ALIGAPGFPQ HGDTGSVEGA
160 170 180 190 200
PQPTDNTFTP KCEIVGKDAL SALARASTKQ CQQEIANVVC LHQAGNLMPK
210 220 230 240 250
SVPRHCQLAG KMSPGVQWEE IRAQQPVGGP PVRIAYMLVV HGRAIRQLKR
260 270 280 290 300
LLKAVYHEQH FFYIHVDKRS NYLYREVVEL AQHYENVRVT PWRMVTIWGG
310 320 330 340 350
ASLLRMYLRS MKDLLEIPGW TWDFFINLSA TDYPTRTNEE LVAFLSKNRD
360 370 380 390 400
KNFLKSHGRD NSRFIKKQGL DRLFHECDSH MWRLGERQIP AGIVVDGGSD
410 420 430 440 450
WFVLTRSFVE YVVYTDDPLV AQLRQFYTYT LLPAESFFHT VLENSPACAS
460 470 480 490 500
LVDNNLRVTN WNRKLGCKCQ YKHIVDWCGC SPNDFKPQDF LRLQQVSRPT
510 520 530 540 550
FFARKFESTV NQEVLEILDF HLYGSYPPST PALKAYWENI YDVADGPGGL
560 570 580 590 600
SDVLLTAYTA FARLSLRHAA TAVSPLATAV CRFEPRGLPS SVHLYFYDDH
610 620 630 640 650
FQGYLVTQAV QPSAQGPAET LEMWLMPQRS LKLLGHSDQA SRLQSLEVGT
660 670 680 690 700
EWDPKERLFR NFGGLLGPLD EPVAMQRWAR GPNLTATVVW IDPTYVVATS
710 720 730 740 750
YDITVDADTE VTQYKPPLSL PLRPGAWTVR LLQFWEPLGE TRFLVLPLTF
760 770 780 790 800
NRKLPLRKDD ASWLHAGPPH NEYMEQSFQG LSGILNLPQA EALEEAARRH
810 820 830 840 850
TELTGSALEA WTDGELSNFW SVAGLCAIGP SACPSLELCR LTSWSSLSPD
860
PKSELGPVKA DGRLR
Length:865
Mass (Da):96,811
Last modified:May 10, 2005 - v3
Checksum:iDA30B77B9807A797
GO

Sequence cautioni

The sequence AAH34082 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94K → N in CAC18567 (Ref. 1) Curated1
Sequence conflicti460N → K in CAC18567 (Ref. 1) Curated1
Sequence conflicti466 – 467GC → AG in CAC18567 (Ref. 1) Curated2
Sequence conflicti478C → S in CAC18567 (Ref. 1) Curated1
Sequence conflicti483N → K in CAC18567 (Ref. 1) Curated1
Sequence conflicti487P → R in CAC18567 (Ref. 1) Curated1
Sequence conflicti504 – 506RKF → GKL in CAC18567 (Ref. 1) Curated3
Sequence conflicti515L → V in CAC18567 (Ref. 1) Curated1
Sequence conflicti666L → V in CAC18567 (Ref. 1) Curated1
Sequence conflicti672P → A in CAC18567 (Ref. 1) Curated1
Sequence conflicti675 – 676MQ → LR in CAC18567 (Ref. 1) Curated2
Sequence conflicti685T → R in CAC18567 (Ref. 1) Curated1
Sequence conflicti768 – 770PPH → QPQ in CAC18567 (Ref. 1) Curated3
Sequence conflicti780G → V in CAC18567 (Ref. 1) Curated1
Sequence conflicti788P → S in CAC18567 (Ref. 1) Curated1
Sequence conflicti799R → Q in CAC18567 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ291751 mRNA. Translation: CAC18567.2.
AL645764 Genomic DNA. Translation: CAI24967.1.
BC034082 mRNA. Translation: AAH34082.1. Different initiation.
CCDSiCCDS48888.1.
RefSeqiNP_665827.2. NM_145828.3.
UniGeneiMm.100638.

Genome annotation databases

EnsembliENSMUST00000116349; ENSMUSP00000112052; ENSMUSG00000020868.
GeneIDi217119.
KEGGimmu:217119.
UCSCiuc007kzi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ291751 mRNA. Translation: CAC18567.2.
AL645764 Genomic DNA. Translation: CAI24967.1.
BC034082 mRNA. Translation: AAH34082.1. Different initiation.
CCDSiCCDS48888.1.
RefSeqiNP_665827.2. NM_145828.3.
UniGeneiMm.100638.

3D structure databases

ProteinModelPortaliQ9EPL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EPL0. 1 interactor.
STRINGi10090.ENSMUSP00000112052.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

PTM databases

iPTMnetiQ9EPL0.
PhosphoSitePlusiQ9EPL0.

Proteomic databases

MaxQBiQ9EPL0.
PaxDbiQ9EPL0.
PRIDEiQ9EPL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116349; ENSMUSP00000112052; ENSMUSG00000020868.
GeneIDi217119.
KEGGimmu:217119.
UCSCiuc007kzi.2. mouse.

Organism-specific databases

CTDi64132.
MGIiMGI:2444797. Xylt2.

Phylogenomic databases

eggNOGiKOG0799. Eukaryota.
ENOG410XQ7M. LUCA.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000070086.
HOVERGENiHBG059443.
InParanoidiQ9EPL0.
KOiK00771.
OMAiVPRHCQL.
OrthoDBiEOG091G020K.
PhylomeDBiQ9EPL0.
TreeFamiTF315534.

Enzyme and pathway databases

UniPathwayiUPA00755.
UPA00756.
ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

ChiTaRSiXylt2. mouse.
PROiQ9EPL0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020868.
CleanExiMM_XYLT2.
ExpressionAtlasiQ9EPL0. baseline and differential.
GenevisibleiQ9EPL0. MM.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
IPR024448. XylT.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
PF12529. Xylo_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXYLT2_MOUSE
AccessioniPrimary (citable) accession number: Q9EPL0
Secondary accession number(s): Q5SUY1, Q8K060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 2, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.