Reviewed,
UniProtKB/Swiss-Prot Q9EPK8 (TRPV4_MOUSE)
Last modified
February 9, 2010.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Transient receptor potential cation channel subfamily V member 4 Short name=TrpV4 Alternative name(s): Osm-9-like TRP channel 4 Short name=OTRPC4 Vanilloid receptor-like channel 2 Vanilloid receptor-like protein 2 Vanilloid receptor-related osmotically-activated channel Short name=VR-OAC Transient receptor potential protein 12 Short name=TRP12 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 871 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Ref.2 |
| Subunit structure | Interacts with calmodulin By similarity. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. Ref.8 Ref.9 |
| Subcellular location | Membrane; Multi-pass membrane protein Probable. |
| Tissue specificity | Expressed heart, liver, kidney and testis. In the central nervous system, expressed in the lamina terminalis (arched vascular organ and neurons of the subfornical organ), median preoptic area, ventral hippocampal commissure, and ependymal cells of the choroid plexus. In the cochlea, expressed in both inner and outer hair cells, and in marginal cells of the cochlear stria vascularis. Expressed in large neurons of the trigeminal ganglion. In the kidney cortex, strongly expressed by epithelial cells of tubules and much weaker in glomeruli. Ref.2 Ref.1 Ref.3 Ref.4 |
| Sequence similarities | Belongs to the transient receptor family. TrpV subfamily. Contains 3 ANK repeats. |
| Sequence caution | The sequence AAG28028.1 differs from that shown. Reason: Frameshift at positions 47, 50, 53, 72 and 73. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 871 | 871 | Transient receptor potential cation channel subfamily V member 4 | PRO_0000215348 | |||||
Regions | |||||||||
| Topological domain | 1 – 465 | 465 | Cytoplasmic Potential | ||||||
| Transmembrane | 466 – 486 | 21 | Potential | ||||||
| Topological domain | 487 – 508 | 22 | Extracellular Potential | ||||||
| Transmembrane | 509 – 529 | 21 | Potential | ||||||
| Topological domain | 530 – 550 | 21 | Cytoplasmic Potential | ||||||
| Transmembrane | 551 – 571 | 21 | Potential | ||||||
| Topological domain | 572 | 1 | Extracellular Potential | ||||||
| Transmembrane | 573 – 593 | 21 | Potential | ||||||
| Topological domain | 594 – 616 | 23 | Cytoplasmic Potential | ||||||
| Transmembrane | 617 – 637 | 21 | Potential | ||||||
| Topological domain | 638 – 690 | 53 | Pore forming Probable | ||||||
| Transmembrane | 691 – 711 | 21 | Potential | ||||||
| Topological domain | 712 – 871 | 160 | Cytoplasmic Potential | ||||||
| Repeat | 237 – 266 | 30 | ANK 1 | ||||||
| Repeat | 284 – 313 | 30 | ANK 2 | ||||||
| Repeat | 369 – 398 | 30 | ANK 3 | ||||||
| Region | 812 – 831 | 20 | Interaction with calmodulin By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 253 | 1 | Phosphotyrosine; by LYN Probable | ||||||
Experimental info | |||||||||
| Mutagenesis | 253 | 1 | Y → F: Abolishes hypotonicity-dependent channel activity. Ref.9 | ||||||
| Mutagenesis | 672 | 1 | D → A: Greatly reduces Ca(2+) permeation and channel rectification; when associated with A-682. Ref.7 | ||||||
| Mutagenesis | 675 | 1 | K → A: No effect on channel pore properties. Ref.7 | ||||||
| Mutagenesis | 680 | 1 | M → A: Impairs Ca(2+) permeation. Ref.7 | ||||||
| Mutagenesis | 682 | 1 | D → A: Greatly reduces Ca(2+) permeation and channel rectification; when associated with A-672. Ref.7 | ||||||
| Sequence conflict | 45 | 1 | G → S in AAG28028. Ref.1 | ||||||
| Sequence conflict | 90 | 1 | L → R Ref.3 | ||||||
| Sequence conflict | 90 | 1 | L → R Ref.5 | ||||||
| Sequence conflict | 137 | 1 | A → T in BAA83731. Ref.4 | ||||||
| Sequence conflict | 210 – 211 | 2 | IP → LQ in BAA83731. Ref.4 | ||||||
| Sequence conflict | 477 | 1 | S → P in AAG28028. Ref.1 | ||||||
| Sequence conflict | 784 | 1 | N → S in BAA83731. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor." Liedtke W.B., Choe Y., Marti-Renom M.A., Bell A.M., Denis C.S., Sali A., Hudspeth A.J., Friedman J.M., Heller S. Cell 103:525-535(2000) [PubMed: 11081638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Hypothalamus. |
| [2] | "Trp12, a novel Trp related protein from kidney." Wissenbach U., Boedding M., Freichel M., Flockerzi V. FEBS Lett. 485:127-134(2000) [PubMed: 11094154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Kidney. |
| [3] | "OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity." Strotmann R., Harteneck C., Nunnenmacher K., Schultz G., Plant T.D. Nat. Cell Biol. 2:695-702(2000) [PubMed: 11025659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: 129/SvEv. |
| [4] | "Impaired pressure sensation in mice lacking TRPV4." Suzuki M., Mizuno A., Kodaira K., Imai M. J. Biol. Chem. 278:22664-22668(2003) [PubMed: 12692122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Kidney. |
| [5] | "Cloning of mouse and human vanilloid receptor-like protein 2 (VRL-2)." Derst C., Schafer M.K. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [7] | "Molecular determinants of permeation through the cation channel TRPV4." Voets T., Prenen J., Vriens J., Watanabe H., Janssens A., Wissenbach U., Bodding M., Droogmans G., Nilius B. J. Biol. Chem. 277:33704-33710(2002) [PubMed: 12093812] [Abstract] Cited for: CHARACTERIZATION OF CHANNEL PORE, MUTAGENESIS OF ASP-672; LYS-675; MET-680 AND ASP-682. |
| [8] | "Microtubule-associated protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4)." Suzuki M., Hirao A., Mizuno A. J. Biol. Chem. 278:51448-51453(2003) [PubMed: 14517216] [Abstract] Cited for: INTERACTION WITH MAP7. |
| [9] | "Regulation of a transient receptor potential (TRP) channel by tyrosine phosphorylation. SRC family kinase-dependent tyrosine phosphorylation of TRPV4 on TYR-253 mediates its response to hypotonic stress." Xu H., Zhao H., Tian W., Yoshida K., Roullet J.B., Cohen D.M. J. Biol. Chem. 278:11520-11527(2003) [PubMed: 12538589] [Abstract] Cited for: INTERACTION WITH LYN; SRC; FYN; HCK; LCK AND YES, PHOSPHORYLATION AT TYR-253, MUTAGENESIS OF TYR-253. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF263522 mRNA. Translation: AAG28028.1. Frameshift. AJ296078 mRNA. Translation: CAC20703.1. AF208026 mRNA. Translation: AAG17543.1. AB021875 mRNA. Translation: BAA83731.2. AF279672 mRNA. Translation: AAK69486.1. Different initiation. BC127052 mRNA. Translation: AAI27053.1. |
| IPI | IPI00112949. |
| RefSeq | NP_071300.2. |
| UniGene | Mm.266450 |
3D structure databases | |
| SMR | Q9EPK8. Positions 149-393. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9EPK8. |
PTM databases | |
| PhosphoSite | Q9EPK8. |
Proteomic databases | |
| PRIDE | Q9EPK8. |
Genome annotation databases | |
| Ensembl | ENSMUST00000071968; ENSMUSP00000071859; ENSMUSG00000014158; Mus musculus. [Genome view] ENSMUST00000112225; ENSMUSP00000107844; ENSMUSG00000014158; Mus musculus. [Genome view] |
| GeneID | 63873. |
| KEGG | mmu:63873. |
Organism-specific databases | |
| CTD | 63873. |
| MGI | MGI:1926945. Trpv4. |
Phylogenomic databases | |
| HOGENOM | HBG446456. |
| HOVERGEN | Q9EPK8. |
| InParanoid | Q9EPK8. |
| OMA | ESTLYES. |
| OrthoDB | EOG9SR0FX. |
| PhylomeDB | Q9EPK8. |
Gene expression databases | |
| ArrayExpress | Q9EPK8. |
| Bgee | Q9EPK8. |
| Genevestigator | Q9EPK8. |
| GermOnline | ENSMUSG00000014158. Mus musculus. |
Family and domain databases | |
| InterPro | IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans. IPR004729. TRP_channel. IPR008347. Vanilpoid_rcpt. IPR008348. Vanilpoid_rcpt_2. [Graphical view] |
| Gene3D | G3DSA:1.25.40.20. ANK. 1 hit. |
| Pfam | PF00023. Ank. 1 hit. PF00520. Ion_trans. 1 hit. [Graphical view] |
| PRINTS | PR01768. TRPVRECEPTOR. PR01769. VRL2RECEPTOR. |
| SMART | SM00248. ANK. 3 hits. [Graphical view] |
| TIGRFAMs | TIGR00870. trp. 1 hit. |
| PROSITE | PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 319803. |
| SOURCE | Search... |
Entry information
| Entry name | TRPV4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9EPK8 Secondary accession number(s): A0JNY0 Q9ES76 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


