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Protein

Nucleotide exchange factor SIL1

Gene

Sil1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5 (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleotide exchange factor SIL1
Gene namesi
Name:Sil1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1932040. Sil1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Mice develop adult-onset ataxia with cerebellar Purkinje cell loss. Affected cells have intracellular protein accumulations in the endoplasmic reticulum and the nucleus.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000022335532 – 465Nucleotide exchange factor SIL1Add BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi197N-linked (GlcNAc...)Sequence analysis1
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9EPK6.
MaxQBiQ9EPK6.
PaxDbiQ9EPK6.
PeptideAtlasiQ9EPK6.
PRIDEiQ9EPK6.

PTM databases

iPTMnetiQ9EPK6.
PhosphoSitePlusiQ9EPK6.
SwissPalmiQ9EPK6.

Expressioni

Tissue specificityi

Expressed in several areas of the brain including the cerebellum, cerebral cortex, cortical neurons, glial cells of white matter, hippocampus, olfactory bulb, Purkinje cells, inferior olive and the choroids plexus. Also expressed in the eye and skeletal muscle.3 Publications

Developmental stagei

Expressed in the developing retina and epithelial cells of the lens at E12.5. Expressed in the developing cerebral cortex at E15.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000024357.
GenevisibleiQ9EPK6. MM.

Interactioni

Subunit structurei

Interacts with HSPA5.1 Publication

Protein-protein interaction databases

BioGridi219881. 2 interactors.
IntActiQ9EPK6. 1 interactor.
MINTiMINT-4128883.
STRINGi10090.ENSMUSP00000025215.

Structurei

3D structure databases

ProteinModelPortaliQ9EPK6.
SMRiQ9EPK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 260Interaction with HSPA5 and localization to the endoplasmic reticulum1 PublicationAdd BLAST260

Sequence similaritiesi

Belongs to the SIL1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2160. Eukaryota.
ENOG410Y90B. LUCA.
GeneTreeiENSGT00510000048102.
HOGENOMiHOG000154326.
HOVERGENiHBG093955.
InParanoidiQ9EPK6.
KOiK14001.
OMAiQEQGWCE.
OrthoDBiEOG091G0196.
PhylomeDBiQ9EPK6.
TreeFamiTF324307.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EPK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQHLPSTR MASPGMLLGL LLTSCLTLCL SCQNSNNFAL TNPEKSIHQE
60 70 80 90 100
SDTKETREEE ELDTEILEVF HPTQEWQTLQ PGQAVPAGSH VRMNLQTGVN
110 120 130 140 150
EVKLQQEDKF QNNLKGFKRG RRLDINANTY TSQDLKSALA KFKEGTEMEN
160 170 180 190 200
SKDELARQAT VKQLFRPIEE LKKEFDELNV VLETDMQIMV RLINKFNSSS
210 220 230 240 250
SSLEEKVAAL FDLEYYVHQM DNAQDLLSFG GLQVVINGLN STEPLVKEYA
260 270 280 290 300
AFVLGAAFSS NPKVQVEAIE GGALQKLLVI LATNQPLPAK KKVLFALCSL
310 320 330 340 350
LRHFPYAQQQ FLKLGGLQVL RSLVQEKSAK VLAVRVVTLL YDLVTEKMFA
360 370 380 390 400
EEEAELTQDS SPEKLQQYRQ VQLLPGLQEQ GWCEITAQLL ALPEHDAREK
410 420 430 440 450
VLQTLGALLT TCRDRYRQDL QLSRTLGRLQ AEYQALASLE LQEGEDDGYF
460
RELLASINSL MKELR
Length:465
Mass (Da):52,430
Last modified:February 21, 2006 - v2
Checksum:iC3E2DB44394F1161
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143K → T in CAC17789 (PubMed:11101517).Curated1
Sequence conflicti152K → Q in CAC17789 (PubMed:11101517).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297884 mRNA. Translation: CAC17789.1.
AK170415 mRNA. Translation: BAE41781.1.
BC016119 mRNA. Translation: AAH16119.1.
BC016466 mRNA. Translation: AAH16466.1.
CCDSiCCDS29140.1.
RefSeqiNP_109674.2. NM_030749.2.
XP_006526428.1. XM_006526365.3.
XP_006526429.1. XM_006526366.3.
XP_011245345.1. XM_011247043.2.
UniGeneiMm.291482.

Genome annotation databases

EnsembliENSMUST00000025215; ENSMUSP00000025215; ENSMUSG00000024357.
GeneIDi81500.
KEGGimmu:81500.
UCSCiuc008emc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297884 mRNA. Translation: CAC17789.1.
AK170415 mRNA. Translation: BAE41781.1.
BC016119 mRNA. Translation: AAH16119.1.
BC016466 mRNA. Translation: AAH16466.1.
CCDSiCCDS29140.1.
RefSeqiNP_109674.2. NM_030749.2.
XP_006526428.1. XM_006526365.3.
XP_006526429.1. XM_006526366.3.
XP_011245345.1. XM_011247043.2.
UniGeneiMm.291482.

3D structure databases

ProteinModelPortaliQ9EPK6.
SMRiQ9EPK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219881. 2 interactors.
IntActiQ9EPK6. 1 interactor.
MINTiMINT-4128883.
STRINGi10090.ENSMUSP00000025215.

PTM databases

iPTMnetiQ9EPK6.
PhosphoSitePlusiQ9EPK6.
SwissPalmiQ9EPK6.

Proteomic databases

EPDiQ9EPK6.
MaxQBiQ9EPK6.
PaxDbiQ9EPK6.
PeptideAtlasiQ9EPK6.
PRIDEiQ9EPK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025215; ENSMUSP00000025215; ENSMUSG00000024357.
GeneIDi81500.
KEGGimmu:81500.
UCSCiuc008emc.2. mouse.

Organism-specific databases

CTDi64374.
MGIiMGI:1932040. Sil1.

Phylogenomic databases

eggNOGiKOG2160. Eukaryota.
ENOG410Y90B. LUCA.
GeneTreeiENSGT00510000048102.
HOGENOMiHOG000154326.
HOVERGENiHBG093955.
InParanoidiQ9EPK6.
KOiK14001.
OMAiQEQGWCE.
OrthoDBiEOG091G0196.
PhylomeDBiQ9EPK6.
TreeFamiTF324307.

Miscellaneous databases

ChiTaRSiSil1. mouse.
PROiQ9EPK6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024357.
GenevisibleiQ9EPK6. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIL1_MOUSE
AccessioniPrimary (citable) accession number: Q9EPK6
Secondary accession number(s): Q91V34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 21, 2006
Last modified: November 2, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.