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Protein

Protein XRP2

Gene

Rp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 96GTPBy similarity2
Nucleotide bindingi112 – 115GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5624138. Trafficking of myristoylated proteins to the cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein XRP2
Gene namesi
Name:Rp2
Synonyms:Rp2h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1277953. Rp2.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Cell projectioncilium By similarity

  • Note: Detected predominantly at the plasma membrane of rod and cone photoreceptors. Not detected in the nucleus.By similarity

GO - Cellular componenti

  • centriole Source: MGI
  • ciliary basal body Source: MGI
  • cytoplasm Source: UniProtKB
  • cytoplasmic vesicle Source: MGI
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • periciliary membrane compartment Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002358042 – 347Protein XRP2Add BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi3S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Myristoylated on Gly-2; which may be required for membrane targeting.Curated
Palmitoylated on Cys-3; which may be required for plasma membrane targeting.Curated

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiQ9EPK2.
PeptideAtlasiQ9EPK2.
PRIDEiQ9EPK2.

PTM databases

iPTMnetiQ9EPK2.
PhosphoSitePlusiQ9EPK2.
SwissPalmiQ9EPK2.

Expressioni

Tissue specificityi

Retina (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000060090.
CleanExiMM_RP2H.
GenevisibleiQ9EPK2. MM.

Interactioni

Subunit structurei

Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with ARL3; interaction is direct and stimulated with the activated GTP-bound form of ARL3 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9EPK2. 2 interactors.
STRINGi10090.ENSMUSP00000033372.

Structurei

3D structure databases

ProteinModelPortaliQ9EPK2.
SMRiQ9EPK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 176C-CAP/cofactor C-likePROSITE-ProRule annotationAdd BLAST156

Sequence similaritiesi

Belongs to the TBCC family.Curated
Contains 1 C-CAP/cofactor C-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2512. Eukaryota.
ENOG410Y28B. LUCA.
GeneTreeiENSGT00530000063741.
HOGENOMiHOG000007790.
HOVERGENiHBG054784.
InParanoidiQ9EPK2.
KOiK18272.
OMAiFFLVQTK.
OrthoDBiEOG091G0K00.
PhylomeDBiQ9EPK2.
TreeFamiTF105832.

Family and domain databases

Gene3Di2.160.20.70. 1 hit.
InterProiIPR013912. Adenylate_cyclase-assoc_CAP_C.
IPR017901. C-CAP_CF_C-like.
IPR016098. CAP/MinC_C.
IPR006599. CARP_motif.
IPR017332. Protein_XRP2.
IPR012945. Tubulin-bd_cofactor_C_dom.
[Graphical view]
PANTHERiPTHR15440:SF0. PTHR15440:SF0. 1 hit.
PfamiPF07986. TBCC. 1 hit.
[Graphical view]
PIRSFiPIRSF037947. Protein_XRP2_. 1 hit.
SMARTiSM00673. CARP. 2 hits.
[Graphical view]
SUPFAMiSSF69340. SSF69340. 1 hit.
PROSITEiPS51329. C_CAP_COFACTOR_C. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EPK2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCCFTKRRK SEKAEGEEEQ PKLYSWDQRE KVDPKDYMFS GLKDETVGRL
60 70 80 90 100
PGKVAGQQFV IQDCENCNIY IFDHSATITI DDCTNCVIFL GPVKGSVFFR
110 120 130 140 150
NCRDCKCTLA CQQFRVRDCR KLEVFLCCAT QPIIESSTNI KFGCFQWYYP
160 170 180 190 200
ELAAQFKDAG LSIFNNIWSH VHDFTPVSGE LNWSLLPENA VVQDYVPIPM
210 220 230 240 250
TEEFKAVRIS TEANRSIVPV SRGQRQKYSD ESCLVVLFAD DYTTANARKL
260 270 280 290 300
IDEMVGKGFS LVQTKEMSMK TEDAQRVFQE KASDFLLLLN KGPVIALEFN
310 320 330 340
GDDAVQECHL IVNGMFNGTK MFVSEKKETA SGDVDSFYNF AEIQMGI
Length:347
Mass (Da):39,377
Last modified:January 23, 2007 - v3
Checksum:iB5F0A3C4C0F4913B
GO
Isoform 2 (identifier: Q9EPK2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-347: MFVSEKKETASGDVDSFYNFAEIQMGI → VLGMEPRTLL...DPLNCGYKWL

Show »
Length:369
Mass (Da):41,957
Checksum:iC8DCE9A745DB4D8C
GO
Isoform 3 (identifier: Q9EPK2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-322: MF → IP
     323-347: Missing.

Show »
Length:322
Mass (Da):36,561
Checksum:i81556E0AA3D151D8
GO
Isoform 4 (identifier: Q9EPK2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-37: Missing.

Show »
Length:310
Mass (Da):34,899
Checksum:i53673DD6440BCB7F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0184811 – 37Missing in isoform 4. 1 PublicationAdd BLAST37
Alternative sequenceiVSP_018482321 – 347MFVSE…IQMGI → VLGMEPRTLLGKKTECQKVW MACQAQTIVTELQCQKRALD PLNCGYKWL in isoform 2. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_018483321 – 322MF → IP in isoform 3. 1 Publication2
Alternative sequenceiVSP_018484323 – 347Missing in isoform 3. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303371 mRNA. Translation: CAC21499.1.
AK033954 mRNA. Translation: BAC28525.1.
AK044002 mRNA. Translation: BAC31734.1.
AK050418 mRNA. Translation: BAC34245.1.
AK076907 mRNA. Translation: BAC36524.1.
AK132713 mRNA. Translation: BAE21316.1.
AK162309 mRNA. Translation: BAE36846.1.
BC049698 mRNA. Translation: AAH49698.1.
CCDSiCCDS30041.1. [Q9EPK2-1]
CCDS72346.1. [Q9EPK2-4]
RefSeqiNP_001277572.1. NM_001290643.1. [Q9EPK2-1]
NP_001277573.1. NM_001290644.1. [Q9EPK2-4]
NP_598430.1. NM_133669.5. [Q9EPK2-1]
XP_017173928.1. XM_017318439.1. [Q9EPK2-4]
UniGeneiMm.288141.

Genome annotation databases

EnsembliENSMUST00000033372; ENSMUSP00000033372; ENSMUSG00000060090. [Q9EPK2-1]
ENSMUST00000115387; ENSMUSP00000111045; ENSMUSG00000060090. [Q9EPK2-4]
ENSMUST00000115391; ENSMUSP00000111049; ENSMUSG00000060090. [Q9EPK2-1]
ENSMUST00000133619; ENSMUSP00000138724; ENSMUSG00000060090. [Q9EPK2-3]
ENSMUST00000134349; ENSMUSP00000138352; ENSMUSG00000060090. [Q9EPK2-2]
GeneIDi19889.
KEGGimmu:19889.
UCSCiuc009ssv.2. mouse. [Q9EPK2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303371 mRNA. Translation: CAC21499.1.
AK033954 mRNA. Translation: BAC28525.1.
AK044002 mRNA. Translation: BAC31734.1.
AK050418 mRNA. Translation: BAC34245.1.
AK076907 mRNA. Translation: BAC36524.1.
AK132713 mRNA. Translation: BAE21316.1.
AK162309 mRNA. Translation: BAE36846.1.
BC049698 mRNA. Translation: AAH49698.1.
CCDSiCCDS30041.1. [Q9EPK2-1]
CCDS72346.1. [Q9EPK2-4]
RefSeqiNP_001277572.1. NM_001290643.1. [Q9EPK2-1]
NP_001277573.1. NM_001290644.1. [Q9EPK2-4]
NP_598430.1. NM_133669.5. [Q9EPK2-1]
XP_017173928.1. XM_017318439.1. [Q9EPK2-4]
UniGeneiMm.288141.

3D structure databases

ProteinModelPortaliQ9EPK2.
SMRiQ9EPK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EPK2. 2 interactors.
STRINGi10090.ENSMUSP00000033372.

PTM databases

iPTMnetiQ9EPK2.
PhosphoSitePlusiQ9EPK2.
SwissPalmiQ9EPK2.

Proteomic databases

PaxDbiQ9EPK2.
PeptideAtlasiQ9EPK2.
PRIDEiQ9EPK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033372; ENSMUSP00000033372; ENSMUSG00000060090. [Q9EPK2-1]
ENSMUST00000115387; ENSMUSP00000111045; ENSMUSG00000060090. [Q9EPK2-4]
ENSMUST00000115391; ENSMUSP00000111049; ENSMUSG00000060090. [Q9EPK2-1]
ENSMUST00000133619; ENSMUSP00000138724; ENSMUSG00000060090. [Q9EPK2-3]
ENSMUST00000134349; ENSMUSP00000138352; ENSMUSG00000060090. [Q9EPK2-2]
GeneIDi19889.
KEGGimmu:19889.
UCSCiuc009ssv.2. mouse. [Q9EPK2-1]

Organism-specific databases

CTDi6102.
MGIiMGI:1277953. Rp2.

Phylogenomic databases

eggNOGiKOG2512. Eukaryota.
ENOG410Y28B. LUCA.
GeneTreeiENSGT00530000063741.
HOGENOMiHOG000007790.
HOVERGENiHBG054784.
InParanoidiQ9EPK2.
KOiK18272.
OMAiFFLVQTK.
OrthoDBiEOG091G0K00.
PhylomeDBiQ9EPK2.
TreeFamiTF105832.

Enzyme and pathway databases

ReactomeiR-MMU-5624138. Trafficking of myristoylated proteins to the cilium.

Miscellaneous databases

PROiQ9EPK2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060090.
CleanExiMM_RP2H.
GenevisibleiQ9EPK2. MM.

Family and domain databases

Gene3Di2.160.20.70. 1 hit.
InterProiIPR013912. Adenylate_cyclase-assoc_CAP_C.
IPR017901. C-CAP_CF_C-like.
IPR016098. CAP/MinC_C.
IPR006599. CARP_motif.
IPR017332. Protein_XRP2.
IPR012945. Tubulin-bd_cofactor_C_dom.
[Graphical view]
PANTHERiPTHR15440:SF0. PTHR15440:SF0. 1 hit.
PfamiPF07986. TBCC. 1 hit.
[Graphical view]
PIRSFiPIRSF037947. Protein_XRP2_. 1 hit.
SMARTiSM00673. CARP. 2 hits.
[Graphical view]
SUPFAMiSSF69340. SSF69340. 1 hit.
PROSITEiPS51329. C_CAP_COFACTOR_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXRP2_MOUSE
AccessioniPrimary (citable) accession number: Q9EPK2
Secondary accession number(s): Q8BLN8, Q8BVQ8, Q8BZP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.