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Q9EPJ9 (ARFG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP-ribosylation factor GTPase-activating protein 1

Short name=ARF GAP 1
Alternative name(s):
ADP-ribosylation factor 1 GTPase-activating protein
Short name=ARF1 GAP
ARF1-directed GTPase-activating protein
Gene names
Name:Arfgap1
Synonyms:Arf1gap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine By similarity.

Subunit structure

Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. It is probably a component of the COPI coat protein complex. The interaction with TMED2 inhibits the GAP activity By similarity.

Subcellular location

Cytoplasm By similarity. Golgi apparatus By similarity. Note: Associates with the Golgi complex By similarity.

Domain

The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes By similarity.

Sequence similarities

Contains 1 Arf-GAP domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Lrrk2Q5S0063EBI-6288020,EBI-2693710

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9EPJ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9EPJ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     259-280: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414ADP-ribosylation factor GTPase-activating protein 1
PRO_0000074191

Regions

Domain7 – 124118Arf-GAP
Zinc finger22 – 4524C4-type

Amino acid modifications

Modified residue1351Phosphothreonine By similarity
Modified residue1891Phosphothreonine By similarity
Modified residue2311N6-acetyllysine By similarity

Natural variations

Alternative sequence259 – 28022Missing in isoform 2.
VSP_011303

Experimental info

Sequence conflict1121R → K in CAC18721. Ref.1
Sequence conflict1881N → K in CAC18721. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: 72A3279D185714C0

FASTA41445,288
        10         20         30         40         50         60 
MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR GLGVHLSFVR 

        70         80         90        100        110        120 
SVTMDKWKDI ELEKMKAGGN AKFREFLETQ DDYEPSWSLQ DKYSSRAAAL FRDKVATLAE 

       130        140        150        160        170        180 
GKEWSLESSP AQNWTPPQPK TLQFTAHRAS GQPQSAAASG DKAFEDWLND DLGSYQGAQE 

       190        200        210        220        230        240 
NRYVGFGNTV PPQKREDDFL NNAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA 

       250        260        270        280        290        300 
SELGHSLNEN VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED 

       310        320        330        340        350        360 
SSDRPLEGHS YQNSSGDNSQ NSNIDQSFWE TFGSAEPPKA KSPSSDSWTC ADASTGRRSS 

       370        380        390        400        410 
DSWDVWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA APSTADEGWD NQNW 

« Hide

Isoform 2 [UniParc].

Checksum: FC2BDDA150130CA2
Show »

FASTA39242,957

References

« Hide 'large scale' references
[1]"Cloning and functional characterisation of mouse ARF1GAP."
Venkateswarlu K.
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Ovary, Pituitary, Placenta and Uterus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ401461 mRNA. Translation: CAC18721.1.
AK030295 mRNA. Translation: BAC26883.1.
AK030520 mRNA. Translation: BAC27002.1.
AK145879 mRNA. Translation: BAE26720.1.
AK168007 mRNA. Translation: BAE39994.1.
BX649560 Genomic DNA. Translation: CAM23227.1.
BX649560 Genomic DNA. Translation: CAM23229.1.
RefSeqNP_001171177.1. NM_001177706.1.
NP_001171178.1. NM_001177707.1.
NP_001171179.1. NM_001177708.1.
NP_001171180.1. NM_001177709.1.
NP_665703.2. NM_145760.3.
XP_006500658.1. XM_006500595.1.
XP_006500661.1. XM_006500598.1.
UniGeneMm.33765.
Mm.470800.

3D structure databases

ProteinModelPortalQ9EPJ9.
SMRQ9EPJ9. Positions 3-181.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230805. 1 interaction.
IntActQ9EPJ9. 1 interaction.

PTM databases

PhosphoSiteQ9EPJ9.

Proteomic databases

PaxDbQ9EPJ9.
PRIDEQ9EPJ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029092; ENSMUSP00000029092; ENSMUSG00000027575. [Q9EPJ9-1]
ENSMUST00000108859; ENSMUSP00000104487; ENSMUSG00000027575. [Q9EPJ9-2]
ENSMUST00000108860; ENSMUSP00000104488; ENSMUSG00000027575. [Q9EPJ9-2]
GeneID228998.
KEGGmmu:228998.
UCSCuc008okj.2. mouse. [Q9EPJ9-1]
uc008okm.2. mouse. [Q9EPJ9-2]

Organism-specific databases

CTD55738.
MGIMGI:2183559. Arfgap1.

Phylogenomic databases

eggNOGCOG5347.
GeneTreeENSGT00670000098104.
HOGENOMHOG000008494.
HOVERGENHBG050562.
InParanoidA8WIR9.
KOK12492.
OMAGWDNQNW.
OrthoDBEOG7C8GH3.
PhylomeDBQ9EPJ9.
TreeFamTF105931.

Gene expression databases

BgeeQ9EPJ9.
GenevestigatorQ9EPJ9.

Family and domain databases

InterProIPR001164. ArfGAP.
[Graphical view]
PfamPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSPR00405. REVINTRACTNG.
SMARTSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio379297.
PROQ9EPJ9.
SOURCESearch...

Entry information

Entry nameARFG1_MOUSE
AccessionPrimary (citable) accession number: Q9EPJ9
Secondary accession number(s): A8WIR9 expand/collapse secondary AC list , A8WIS1, Q3TI52, Q8BMM6, Q8BMQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot