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Protein

ADP-ribosylation factor GTPase-activating protein 1

Gene

Arfgap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 4524C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ARF GTPase activator activity Source: InterPro
  2. GTPase activator activity Source: MGI
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of GTPase activity Source: MGI
  2. protein transport Source: UniProtKB-KW
  3. regulation of ARF GTPase activity Source: InterPro
  4. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_106572. XBP1(S) activates chaperone genes.
REACT_227904. COPI Mediated Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 1
Short name:
ARF GAP 1
Alternative name(s):
ADP-ribosylation factor 1 GTPase-activating protein
Short name:
ARF1 GAP
ARF1-directed GTPase-activating protein
Gene namesi
Name:Arfgap1
Synonyms:Arf1gap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2183559. Arfgap1.

Subcellular locationi

Cytoplasm By similarity. Golgi apparatus By similarity
Note: Associates with the Golgi complex.By similarity

GO - Cellular componenti

  1. Golgi membrane Source: Ensembl
  2. neuronal postsynaptic density Source: MGI
  3. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414ADP-ribosylation factor GTPase-activating protein 1PRO_0000074191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351PhosphothreonineBy similarity
Modified residuei189 – 1891PhosphothreonineBy similarity
Modified residuei231 – 2311N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9EPJ9.
PaxDbiQ9EPJ9.
PRIDEiQ9EPJ9.

PTM databases

PhosphoSiteiQ9EPJ9.

Expressioni

Gene expression databases

BgeeiQ9EPJ9.
ExpressionAtlasiQ9EPJ9. baseline and differential.
GenevestigatoriQ9EPJ9.

Interactioni

Subunit structurei

Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. It is probably a component of the COPI coat protein complex. The interaction with TMED2 inhibits the GAP activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Lrrk2Q5S0063EBI-6288020,EBI-2693710

Protein-protein interaction databases

BioGridi230805. 1 interaction.
IntActiQ9EPJ9. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9EPJ9.
SMRiQ9EPJ9. Positions 3-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 124118Arf-GAPPROSITE-ProRule annotationAdd
BLAST

Domaini

The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes (By similarity).By similarity

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 4524C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00670000098104.
HOGENOMiHOG000008494.
HOVERGENiHBG050562.
InParanoidiQ9EPJ9.
KOiK12492.
OrthoDBiEOG7C8GH3.
PhylomeDBiQ9EPJ9.
TreeFamiTF105931.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9EPJ9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR
60 70 80 90 100
GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLETQ DDYEPSWSLQ
110 120 130 140 150
DKYSSRAAAL FRDKVATLAE GKEWSLESSP AQNWTPPQPK TLQFTAHRAS
160 170 180 190 200
GQPQSAAASG DKAFEDWLND DLGSYQGAQE NRYVGFGNTV PPQKREDDFL
210 220 230 240 250
NNAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA SELGHSLNEN
260 270 280 290 300
VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED
310 320 330 340 350
SSDRPLEGHS YQNSSGDNSQ NSNIDQSFWE TFGSAEPPKA KSPSSDSWTC
360 370 380 390 400
ADASTGRRSS DSWDVWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA
410
APSTADEGWD NQNW
Length:414
Mass (Da):45,288
Last modified:August 16, 2004 - v2
Checksum:i72A3279D185714C0
GO
Isoform 2 (identifier: Q9EPJ9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-280: Missing.

Note: No experimental confirmation available.

Show »
Length:392
Mass (Da):42,957
Checksum:iFC2BDDA150130CA2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121R → K in CAC18721. 1 PublicationCurated
Sequence conflicti188 – 1881N → K in CAC18721. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei259 – 28022Missing in isoform 2. 1 PublicationVSP_011303Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401461 mRNA. Translation: CAC18721.1.
AK030295 mRNA. Translation: BAC26883.1.
AK030520 mRNA. Translation: BAC27002.1.
AK145879 mRNA. Translation: BAE26720.1.
AK168007 mRNA. Translation: BAE39994.1.
BX649560 Genomic DNA. Translation: CAM23227.1.
BX649560 Genomic DNA. Translation: CAM23229.1.
CCDSiCCDS17190.1. [Q9EPJ9-1]
CCDS50843.1. [Q9EPJ9-2]
RefSeqiNP_001171177.1. NM_001177706.1. [Q9EPJ9-2]
NP_001171178.1. NM_001177707.1.
NP_001171179.1. NM_001177708.1.
NP_001171180.1. NM_001177709.1. [Q9EPJ9-2]
NP_665703.2. NM_145760.3. [Q9EPJ9-1]
XP_006500658.1. XM_006500595.1. [Q9EPJ9-1]
XP_006500661.1. XM_006500598.1. [Q9EPJ9-2]
UniGeneiMm.33765.
Mm.470800.

Genome annotation databases

EnsembliENSMUST00000029092; ENSMUSP00000029092; ENSMUSG00000027575. [Q9EPJ9-1]
ENSMUST00000108859; ENSMUSP00000104487; ENSMUSG00000027575. [Q9EPJ9-2]
ENSMUST00000108860; ENSMUSP00000104488; ENSMUSG00000027575. [Q9EPJ9-2]
GeneIDi228998.
KEGGimmu:228998.
UCSCiuc008okj.2. mouse. [Q9EPJ9-1]
uc008okm.2. mouse. [Q9EPJ9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401461 mRNA. Translation: CAC18721.1.
AK030295 mRNA. Translation: BAC26883.1.
AK030520 mRNA. Translation: BAC27002.1.
AK145879 mRNA. Translation: BAE26720.1.
AK168007 mRNA. Translation: BAE39994.1.
BX649560 Genomic DNA. Translation: CAM23227.1.
BX649560 Genomic DNA. Translation: CAM23229.1.
CCDSiCCDS17190.1. [Q9EPJ9-1]
CCDS50843.1. [Q9EPJ9-2]
RefSeqiNP_001171177.1. NM_001177706.1. [Q9EPJ9-2]
NP_001171178.1. NM_001177707.1.
NP_001171179.1. NM_001177708.1.
NP_001171180.1. NM_001177709.1. [Q9EPJ9-2]
NP_665703.2. NM_145760.3. [Q9EPJ9-1]
XP_006500658.1. XM_006500595.1. [Q9EPJ9-1]
XP_006500661.1. XM_006500598.1. [Q9EPJ9-2]
UniGeneiMm.33765.
Mm.470800.

3D structure databases

ProteinModelPortaliQ9EPJ9.
SMRiQ9EPJ9. Positions 3-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230805. 1 interaction.
IntActiQ9EPJ9. 1 interaction.

PTM databases

PhosphoSiteiQ9EPJ9.

Proteomic databases

MaxQBiQ9EPJ9.
PaxDbiQ9EPJ9.
PRIDEiQ9EPJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029092; ENSMUSP00000029092; ENSMUSG00000027575. [Q9EPJ9-1]
ENSMUST00000108859; ENSMUSP00000104487; ENSMUSG00000027575. [Q9EPJ9-2]
ENSMUST00000108860; ENSMUSP00000104488; ENSMUSG00000027575. [Q9EPJ9-2]
GeneIDi228998.
KEGGimmu:228998.
UCSCiuc008okj.2. mouse. [Q9EPJ9-1]
uc008okm.2. mouse. [Q9EPJ9-2]

Organism-specific databases

CTDi55738.
MGIiMGI:2183559. Arfgap1.

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00670000098104.
HOGENOMiHOG000008494.
HOVERGENiHBG050562.
InParanoidiQ9EPJ9.
KOiK12492.
OrthoDBiEOG7C8GH3.
PhylomeDBiQ9EPJ9.
TreeFamiTF105931.

Enzyme and pathway databases

ReactomeiREACT_106572. XBP1(S) activates chaperone genes.
REACT_227904. COPI Mediated Transport.

Miscellaneous databases

NextBioi379297.
PROiQ9EPJ9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9EPJ9.
ExpressionAtlasiQ9EPJ9. baseline and differential.
GenevestigatoriQ9EPJ9.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional characterisation of mouse ARF1GAP."
    Venkateswarlu K.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Ovary, Pituitary, Placenta and Uterus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiARFG1_MOUSE
AccessioniPrimary (citable) accession number: Q9EPJ9
Secondary accession number(s): A8WIR9
, A8WIS1, Q3TI52, Q8BMM6, Q8BMQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: August 16, 2004
Last modified: February 4, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.