Potassium voltage-gated channel subfamily H member 5

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Q9EPI9 (KCNH5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily H member 5

Alternative name(s):
Ether-a-go-go potassium channel 2
Short name=rEAG2
Voltage-gated potassium channel subunit Kv10.2

Gene names

Name:	Kcnh5
Synonyms:	Eag2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	988 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.
Subunit structure	The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH1/EAG By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Detected in adult testis and in embryonic and adult brain, but not in other tissues. Highly expressed in specific brain areas, such as neocortex, olfactory bulb, primary olfactory cortex and brain stem. In cortex, expression is concentrated in a narrow band toward the middle lamella (layer IV). Moderately expressed in spinal cord, dorsal thalamic nuclei, medial hypothalamus, colliculus, lateral lemniscus, pontine nuclei and Islands of Calleja.
Miscellaneous	The channel is down-regulated to 10% residual activity by 400 nano molar cytosolic calcium ions.
Sequence similarities	Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.2/KCNH5 sub-subfamily. [View classification] Contains 1 cyclic nucleotide-binding domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Calmodulin-binding Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro signal transduction by phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	phosphorelay sensor kinase activity Inferred from electronic annotation. Source: InterPro voltage-gated potassium channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 988

988

Potassium voltage-gated channel subfamily H member 5

PRO_0000054012

Regions

Topological domain

1 – 217

217

Cytoplasmic Potential

Transmembrane

218 – 238

Helical; Name=Segment S1; Potential

Topological domain

239 – 243

Extracellular Potential

Transmembrane

244 – 264

Helical; Name=Segment S2; Potential

Topological domain

265 – 291

Cytoplasmic Potential

Transmembrane

292 – 312

Helical; Name=Segment S3; Potential

Topological domain

313 – 319

Extracellular Potential

Transmembrane

320 – 340

Helical; Voltage-sensor; Name=Segment S4; Potential

Topological domain

341 – 346

Cytoplasmic Potential

Transmembrane

347 – 367

Helical; Name=Segment S5; Potential

Topological domain

368 – 419

Extracellular Potential

Intramembrane

420 – 440

Pore-forming; Name=Segment H5; Potential

Topological domain

441 – 446

Extracellular Potential

Transmembrane

447 – 467

Helical; Name=Segment S6; Potential

Topological domain

468 – 988

521

Cytoplasmic Potential

Domain

14 – 86

PAS

Domain

91 – 143

PAC

Nucleotide binding

550 – 667

118

cNMP

Region

704 – 715

Calmodulin-binding Potential

Region

909 – 948

CAD (involved in subunit assembly) By similarity

Motif

432 – 437

Selectivity filter By similarity

Amino acid modifications

Glycosylation

403

N-linked (GlcNAc...) Potential

Cross-link

785

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict

397

G → R in AAF19354. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9EPI9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 888AE96759F64C4A
Show »

FASTA

988

111,830

        10         20         30         40         50         60 
MPGGKRGLVA PQNTFLENIV RRSSESSFLL GNAQIVDWPV VYSNDGFCKL SGYHRADVMQ 

        70         80         90        100        110        120 
KSSTCSFMYG ELTDKKTIEK VRQTFDNYES NCFEVLLYKK NRTPVWFYMQ IAPIRNEHEK 

       130        140        150        160        170        180 
VVLFLCTFKD ITLFKQPIED DSTKGWTKFA RLTRALTNSR SVLQQLTPMN KTETVHKHSR 

       190        200        210        220        230        240 
LAEVLQLGSD ILPQYKQEAP KTPPHIILHY CAFKTTWDWV ILILTFYTAI MVPYNVSFKT 

       250        260        270        280        290        300 
KQNNIAWLVL DSVVDVIFLV DIVLNFHTTF VGPGGEVISD PKLIRMNYLK TWFVIDLLSC 

       310        320        330        340        350        360 
LPYDIINAFE NVDEGISSLF SSLKVVRLLR LGRVARKLDH YLEYGAAVLV LLVCVFGLVA 

       370        380        390        400        410        420 
HWLACIWYSI GDYEVIDEVT NTIQIDSWLY QLALSIGTPY RYNTSAGIWE GGPSKDSLYV 

       430        440        450        460        470        480 
SSLYFTMTSL TTIGFGNIAP TTDVEKMFSV AMMMVGSLLY ATIFGNVTTI FQQMYANTNR 

       490        500        510        520        530        540 
YHEMLNNVRD FLKLYQVPKG LSERVMDYIV STWSMSKGID TEKVLSICPK DMRADICVHL 

       550        560        570        580        590        600 
NRKVFNEHPA FRLASDGCLR ALAVEFQTIH CAPGDLIYHA GESVDALCFV VSGSLEVIQD 

       610        620        630        640        650        660 
EEVVAILGKG DVFGDIFWKE TTLAHACANV RALTYCDLHI IKREALLKVL DFYTAFANSF 

       670        680        690        700        710        720 
SRNLTLTCNL RKRIIFRKIS DVKKEEEERL RQKNEVTLSI PVDHPVRKLF QKFKQQKELR 

       730        740        750        760        770        780 
NQGSAQSDPE RSQLQVESRP LQNGASITGT SVVTVSQITP IQTSLAYVKT SETLKQNNRD 

       790        800        810        820        830        840 
AMELKPNGGA EPKCLKVNSP IRMKNGNGKG WLRLKNNMGA HEEKKEEWNN VTKAESMGLL 

       850        860        870        880        890        900 
SEDPKGSDSE NSVTKNPLRK TDSCDSGITK SDLRLDKAGE ARSPLEHSPS QADAKHPFYP 

       910        920        930        940        950        960 
IPEQALQTTL QEVKHELKED IQLLSCRMTA LEKQVAEILK LLSEKSVPQT SSPKPQIPLQ 

       970        980 
VPPQIPCQDI FSVSRPESPE SDKDEINF

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References

[1]	"Cloning of components of a novel subthreshold-activating K+ channel with a unique pattern of expression in the cerebral cortex." Saganich M.J., Vega-Saenz de Miera E., Nadal M.S., Baker H., Coetzee W.A., Rudy B. J. Neurosci. 19:10789-10802(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Thalamus.
[2]	"Cloning and functional expression of rat eag2, a new member of the ether-a-go-go family of potassium channels and comparison of its distribution with that of eag1." Ludwig J., Weseloh R., Karschin C., Liu Q., Netzer R., Engeland B., Stansfeld C., Pongs O. Mol. Cell. Neurosci. 16:59-70(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cerebellum.
[3]	"Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia." Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E. J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 220-360. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF185637 mRNA. Translation: AAF19354.1. AJ250280 mRNA. Translation: CAC20863.1. AF073891 mRNA. Translation: AAC61521.1.
IPI	IPI00189118.
RefSeq	NP_598294.1. NM_133610.2.
UniGene	Rn.48753.
3D structure databases
ProteinModelPortal	Q9EPI9.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000013275.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000013275; ENSRNOP00000013275; ENSRNOG00000009542.
GeneID	171146.
KEGG	rno:171146.
UCSC	RGD:621417. rat.
Organism-specific databases
CTD	27133.
RGD	621417. Kcnh5.
Phylogenomic databases
eggNOG	COG2202.
GeneTree	ENSGT00680000099663.
HOGENOM	HOG000230794.
HOVERGEN	HBG101348.
InParanoid	Q9EPI9.
KO	K04908.
OrthoDB	EOG47PX5C.
Gene expression databases
Genevestigator	Q9EPI9.
GermOnline	ENSRNOG00000009542. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
InterPro	IPR018490. cNMP-bd-like. IPR000595. cNMP-bd_dom. IPR005821. Ion_trans_dom. IPR003949. K_chnl_volt-dep_EAG. IPR003938. K_chnl_volt-dep_EAG/ELK/ERG. IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR014710. RmlC-like_jellyroll. [Graphical view]
Pfam	PF00027. cNMP_binding. 1 hit. PF00520. Ion_trans. 1 hit. [Graphical view]
PRINTS	PR01463. EAGCHANLFMLY. PR01464. EAGCHANNEL.
SMART	SM00100. cNMP. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 1 hit.
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS00888. CNMP_BINDING_1. False negative. PS00889. CNMP_BINDING_2. False negative. PS50042. CNMP_BINDING_3. 1 hit. PS50113. PAC. 1 hit. PS50112. PAS. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	621956.

Entry information

Entry name

KCNH5_RAT

Accession

Primary (citable) accession number: Q9EPI9
Secondary accession number(s): O88893, Q9QXT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents