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Q9EPI9 (KCNH5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily H member 5
Alternative name(s):
Ether-a-go-go potassium channel 2
Short name=rEAG2
Voltage-gated potassium channel subunit Kv10.2
Gene names
Name:Kcnh5
Synonyms:Eag2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length988 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Subunit structure

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH1/EAG By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Detected in adult testis and in embryonic and adult brain, but not in other tissues. Highly expressed in specific brain areas, such as neocortex, olfactory bulb, primary olfactory cortex and brain stem. In cortex, expression is concentrated in a narrow band toward the middle lamella (layer IV). Moderately expressed in spinal cord, dorsal thalamic nuclei, medial hypothalamus, colliculus, lateral lemniscus, pontine nuclei and Islands of Calleja.

Miscellaneous

The channel is down-regulated to 10% residual activity by 400 nano molar cytosolic calcium ions.

Sequence similarities

Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.2/KCNH5 sub-subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 988988Potassium voltage-gated channel subfamily H member 5
PRO_0000054012

Regions

Topological domain1 – 217217Cytoplasmic Potential
Transmembrane218 – 23821Helical; Name=Segment S1; Potential
Topological domain239 – 2435Extracellular Potential
Transmembrane244 – 26421Helical; Name=Segment S2; Potential
Topological domain265 – 29127Cytoplasmic Potential
Transmembrane292 – 31221Helical; Name=Segment S3; Potential
Topological domain313 – 3197Extracellular Potential
Transmembrane320 – 34021Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain341 – 3466Cytoplasmic Potential
Transmembrane347 – 36721Helical; Name=Segment S5; Potential
Topological domain368 – 41952Extracellular Potential
Intramembrane420 – 44021Pore-forming; Name=Segment H5; Potential
Topological domain441 – 4466Extracellular Potential
Transmembrane447 – 46721Helical; Name=Segment S6; Potential
Topological domain468 – 988521Cytoplasmic Potential
Domain14 – 8673PAS
Domain91 – 14353PAC
Nucleotide binding550 – 667118cNMP
Region704 – 71512Calmodulin-binding Potential
Region909 – 94840CAD (involved in subunit assembly) By similarity
Motif432 – 4376Selectivity filter By similarity

Amino acid modifications

Glycosylation4031N-linked (GlcNAc...) Potential
Cross-link785Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict3971G → R in AAF19354. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9EPI9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 888AE96759F64C4A

FASTA988111,830
        10         20         30         40         50         60 
MPGGKRGLVA PQNTFLENIV RRSSESSFLL GNAQIVDWPV VYSNDGFCKL SGYHRADVMQ 

        70         80         90        100        110        120 
KSSTCSFMYG ELTDKKTIEK VRQTFDNYES NCFEVLLYKK NRTPVWFYMQ IAPIRNEHEK 

       130        140        150        160        170        180 
VVLFLCTFKD ITLFKQPIED DSTKGWTKFA RLTRALTNSR SVLQQLTPMN KTETVHKHSR 

       190        200        210        220        230        240 
LAEVLQLGSD ILPQYKQEAP KTPPHIILHY CAFKTTWDWV ILILTFYTAI MVPYNVSFKT 

       250        260        270        280        290        300 
KQNNIAWLVL DSVVDVIFLV DIVLNFHTTF VGPGGEVISD PKLIRMNYLK TWFVIDLLSC 

       310        320        330        340        350        360 
LPYDIINAFE NVDEGISSLF SSLKVVRLLR LGRVARKLDH YLEYGAAVLV LLVCVFGLVA 

       370        380        390        400        410        420 
HWLACIWYSI GDYEVIDEVT NTIQIDSWLY QLALSIGTPY RYNTSAGIWE GGPSKDSLYV 

       430        440        450        460        470        480 
SSLYFTMTSL TTIGFGNIAP TTDVEKMFSV AMMMVGSLLY ATIFGNVTTI FQQMYANTNR 

       490        500        510        520        530        540 
YHEMLNNVRD FLKLYQVPKG LSERVMDYIV STWSMSKGID TEKVLSICPK DMRADICVHL 

       550        560        570        580        590        600 
NRKVFNEHPA FRLASDGCLR ALAVEFQTIH CAPGDLIYHA GESVDALCFV VSGSLEVIQD 

       610        620        630        640        650        660 
EEVVAILGKG DVFGDIFWKE TTLAHACANV RALTYCDLHI IKREALLKVL DFYTAFANSF 

       670        680        690        700        710        720 
SRNLTLTCNL RKRIIFRKIS DVKKEEEERL RQKNEVTLSI PVDHPVRKLF QKFKQQKELR 

       730        740        750        760        770        780 
NQGSAQSDPE RSQLQVESRP LQNGASITGT SVVTVSQITP IQTSLAYVKT SETLKQNNRD 

       790        800        810        820        830        840 
AMELKPNGGA EPKCLKVNSP IRMKNGNGKG WLRLKNNMGA HEEKKEEWNN VTKAESMGLL 

       850        860        870        880        890        900 
SEDPKGSDSE NSVTKNPLRK TDSCDSGITK SDLRLDKAGE ARSPLEHSPS QADAKHPFYP 

       910        920        930        940        950        960 
IPEQALQTTL QEVKHELKED IQLLSCRMTA LEKQVAEILK LLSEKSVPQT SSPKPQIPLQ 

       970        980 
VPPQIPCQDI FSVSRPESPE SDKDEINF 

« Hide

References

[1]"Cloning of components of a novel subthreshold-activating K+ channel with a unique pattern of expression in the cerebral cortex."
Saganich M.J., Vega-Saenz de Miera E., Nadal M.S., Baker H., Coetzee W.A., Rudy B.
J. Neurosci. 19:10789-10802(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Thalamus.
[2]"Cloning and functional expression of rat eag2, a new member of the ether-a-go-go family of potassium channels and comparison of its distribution with that of eag1."
Ludwig J., Weseloh R., Karschin C., Liu Q., Netzer R., Engeland B., Stansfeld C., Pongs O.
Mol. Cell. Neurosci. 16:59-70(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cerebellum.
[3]"Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia."
Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E.
J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 220-360.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF185637 mRNA. Translation: AAF19354.1.
AJ250280 mRNA. Translation: CAC20863.1.
AF073891 mRNA. Translation: AAC61521.1.
RefSeqNP_598294.1. NM_133610.2.
UniGeneRn.48753.

3D structure databases

ProteinModelPortalQ9EPI9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000013275.

Chemistry

GuidetoPHARMACOLOGY571.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000013275; ENSRNOP00000013275; ENSRNOG00000009542.
GeneID171146.
KEGGrno:171146.
UCSCRGD:621417. rat.

Organism-specific databases

CTD27133.
RGD621417. Kcnh5.

Phylogenomic databases

eggNOGCOG2202.
GeneTreeENSGT00680000099663.
HOGENOMHOG000230794.
HOVERGENHBG101348.
InParanoidQ9EPI9.
KOK04908.
OMAPKCLKVN.
OrthoDBEOG7QG43V.
PhylomeDBQ9EPI9.
TreeFamTF313130.

Gene expression databases

GenevestigatorQ9EPI9.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003949. K_chnl_volt-dep_EAG.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSPR01463. EAGCHANLFMLY.
PR01464. EAGCHANNEL.
SMARTSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio621956.
PROQ9EPI9.

Entry information

Entry nameKCNH5_RAT
AccessionPrimary (citable) accession number: Q9EPI9
Secondary accession number(s): O88893, Q9QXT2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families