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Q9EPI9

- KCNH5_RAT

UniProt

Q9EPI9 - KCNH5_RAT

Protein

Potassium voltage-gated channel subfamily H member 5

Gene

Kcnh5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi550 – 667118cNMPAdd
    BLAST

    GO - Molecular functioni

    1. phosphorelay sensor kinase activity Source: InterPro
    2. voltage-gated potassium channel activity Source: RefGenome

    GO - Biological processi

    1. potassium ion transmembrane transport Source: RefGenome
    2. regulation of G2/M transition of mitotic cell cycle Source: Ensembl
    3. regulation of membrane potential Source: RefGenome

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Calmodulin-binding, Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199159. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily H member 5
    Alternative name(s):
    Ether-a-go-go potassium channel 2
    Short name:
    rEAG2
    Voltage-gated potassium channel subunit Kv10.2
    Gene namesi
    Name:Kcnh5
    Synonyms:Eag2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi621417. Kcnh5.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. integral component of plasma membrane Source: RefGenome
    3. intracellular Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 988988Potassium voltage-gated channel subfamily H member 5PRO_0000054012Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis
    Cross-linki785 – 785Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Glycoprotein, Isopeptide bond, Ubl conjugation

    Expressioni

    Tissue specificityi

    Detected in adult testis and in embryonic and adult brain, but not in other tissues. Highly expressed in specific brain areas, such as neocortex, olfactory bulb, primary olfactory cortex and brain stem. In cortex, expression is concentrated in a narrow band toward the middle lamella (layer IV). Moderately expressed in spinal cord, dorsal thalamic nuclei, medial hypothalamus, colliculus, lateral lemniscus, pontine nuclei and Islands of Calleja.

    Gene expression databases

    GenevestigatoriQ9EPI9.

    Interactioni

    Subunit structurei

    The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH1/EAG By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000013275.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EPI9.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 217217CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini239 – 2435ExtracellularSequence Analysis
    Topological domaini265 – 29127CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini313 – 3197ExtracellularSequence Analysis
    Topological domaini341 – 3466CytoplasmicSequence Analysis
    Topological domaini368 – 41952ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini441 – 4466ExtracellularSequence Analysis
    Topological domaini468 – 988521CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei420 – 44021Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei218 – 23821Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei244 – 26421Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei292 – 31221Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei320 – 34021Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei347 – 36721Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei447 – 46721Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 8673PASAdd
    BLAST
    Domaini91 – 14353PACPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni704 – 71512Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni909 – 94840CAD (involved in subunit assembly)By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi432 – 4376Selectivity filterBy similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
    Contains 1 PAS (PER-ARNT-SIM) domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2202.
    GeneTreeiENSGT00680000099663.
    HOGENOMiHOG000230794.
    HOVERGENiHBG101348.
    InParanoidiQ9EPI9.
    KOiK04908.
    OMAiGSTQSDP.
    OrthoDBiEOG7QG43V.
    PhylomeDBiQ9EPI9.
    TreeFamiTF313130.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003949. K_chnl_volt-dep_EAG.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view]
    PRINTSiPR01463. EAGCHANLFMLY.
    PR01464. EAGCHANNEL.
    SMARTiSM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EPI9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGGKRGLVA PQNTFLENIV RRSSESSFLL GNAQIVDWPV VYSNDGFCKL    50
    SGYHRADVMQ KSSTCSFMYG ELTDKKTIEK VRQTFDNYES NCFEVLLYKK 100
    NRTPVWFYMQ IAPIRNEHEK VVLFLCTFKD ITLFKQPIED DSTKGWTKFA 150
    RLTRALTNSR SVLQQLTPMN KTETVHKHSR LAEVLQLGSD ILPQYKQEAP 200
    KTPPHIILHY CAFKTTWDWV ILILTFYTAI MVPYNVSFKT KQNNIAWLVL 250
    DSVVDVIFLV DIVLNFHTTF VGPGGEVISD PKLIRMNYLK TWFVIDLLSC 300
    LPYDIINAFE NVDEGISSLF SSLKVVRLLR LGRVARKLDH YLEYGAAVLV 350
    LLVCVFGLVA HWLACIWYSI GDYEVIDEVT NTIQIDSWLY QLALSIGTPY 400
    RYNTSAGIWE GGPSKDSLYV SSLYFTMTSL TTIGFGNIAP TTDVEKMFSV 450
    AMMMVGSLLY ATIFGNVTTI FQQMYANTNR YHEMLNNVRD FLKLYQVPKG 500
    LSERVMDYIV STWSMSKGID TEKVLSICPK DMRADICVHL NRKVFNEHPA 550
    FRLASDGCLR ALAVEFQTIH CAPGDLIYHA GESVDALCFV VSGSLEVIQD 600
    EEVVAILGKG DVFGDIFWKE TTLAHACANV RALTYCDLHI IKREALLKVL 650
    DFYTAFANSF SRNLTLTCNL RKRIIFRKIS DVKKEEEERL RQKNEVTLSI 700
    PVDHPVRKLF QKFKQQKELR NQGSAQSDPE RSQLQVESRP LQNGASITGT 750
    SVVTVSQITP IQTSLAYVKT SETLKQNNRD AMELKPNGGA EPKCLKVNSP 800
    IRMKNGNGKG WLRLKNNMGA HEEKKEEWNN VTKAESMGLL SEDPKGSDSE 850
    NSVTKNPLRK TDSCDSGITK SDLRLDKAGE ARSPLEHSPS QADAKHPFYP 900
    IPEQALQTTL QEVKHELKED IQLLSCRMTA LEKQVAEILK LLSEKSVPQT 950
    SSPKPQIPLQ VPPQIPCQDI FSVSRPESPE SDKDEINF 988
    Length:988
    Mass (Da):111,830
    Last modified:March 1, 2001 - v1
    Checksum:i888AE96759F64C4A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti397 – 3971G → R in AAF19354. (PubMed:10594062)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF185637 mRNA. Translation: AAF19354.1.
    AJ250280 mRNA. Translation: CAC20863.1.
    AF073891 mRNA. Translation: AAC61521.1.
    RefSeqiNP_598294.1. NM_133610.2.
    UniGeneiRn.48753.

    Genome annotation databases

    EnsembliENSRNOT00000013275; ENSRNOP00000013275; ENSRNOG00000009542.
    GeneIDi171146.
    KEGGirno:171146.
    UCSCiRGD:621417. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF185637 mRNA. Translation: AAF19354.1 .
    AJ250280 mRNA. Translation: CAC20863.1 .
    AF073891 mRNA. Translation: AAC61521.1 .
    RefSeqi NP_598294.1. NM_133610.2.
    UniGenei Rn.48753.

    3D structure databases

    ProteinModelPortali Q9EPI9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000013275.

    Chemistry

    GuidetoPHARMACOLOGYi 571.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000013275 ; ENSRNOP00000013275 ; ENSRNOG00000009542 .
    GeneIDi 171146.
    KEGGi rno:171146.
    UCSCi RGD:621417. rat.

    Organism-specific databases

    CTDi 27133.
    RGDi 621417. Kcnh5.

    Phylogenomic databases

    eggNOGi COG2202.
    GeneTreei ENSGT00680000099663.
    HOGENOMi HOG000230794.
    HOVERGENi HBG101348.
    InParanoidi Q9EPI9.
    KOi K04908.
    OMAi GSTQSDP.
    OrthoDBi EOG7QG43V.
    PhylomeDBi Q9EPI9.
    TreeFami TF313130.

    Enzyme and pathway databases

    Reactomei REACT_199159. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 621956.
    PROi Q9EPI9.

    Gene expression databases

    Genevestigatori Q9EPI9.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003949. K_chnl_volt-dep_EAG.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view ]
    PRINTSi PR01463. EAGCHANLFMLY.
    PR01464. EAGCHANNEL.
    SMARTi SM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of components of a novel subthreshold-activating K+ channel with a unique pattern of expression in the cerebral cortex."
      Saganich M.J., Vega-Saenz de Miera E., Nadal M.S., Baker H., Coetzee W.A., Rudy B.
      J. Neurosci. 19:10789-10802(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Thalamus.
    2. "Cloning and functional expression of rat eag2, a new member of the ether-a-go-go family of potassium channels and comparison of its distribution with that of eag1."
      Ludwig J., Weseloh R., Karschin C., Liu Q., Netzer R., Engeland B., Stansfeld C., Pongs O.
      Mol. Cell. Neurosci. 16:59-70(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Cerebellum.
    3. "Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia."
      Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E.
      J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 220-360.
      Tissue: Brain.

    Entry informationi

    Entry nameiKCNH5_RAT
    AccessioniPrimary (citable) accession number: Q9EPI9
    Secondary accession number(s): O88893, Q9QXT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The channel is down-regulated to 10% residual activity by 400 nano molar cytosolic calcium ions.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3