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Q9EPI9

- KCNH5_RAT

UniProt

Q9EPI9 - KCNH5_RAT

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Protein

Potassium voltage-gated channel subfamily H member 5

Gene

Kcnh5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi550 – 667118cNMPAdd
BLAST

GO - Molecular functioni

  1. phosphorelay sensor kinase activity Source: InterPro
  2. voltage-gated potassium channel activity Source: RefGenome

GO - Biological processi

  1. potassium ion transmembrane transport Source: RefGenome
  2. regulation of G2/M transition of mitotic cell cycle Source: Ensembl
  3. regulation of membrane potential Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calmodulin-binding, Potassium

Enzyme and pathway databases

ReactomeiREACT_199159. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily H member 5
Alternative name(s):
Ether-a-go-go potassium channel 2
Short name:
rEAG2
Voltage-gated potassium channel subunit Kv10.2
Gene namesi
Name:Kcnh5
Synonyms:Eag2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi621417. Kcnh5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 217217CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei218 – 23821Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Topological domaini239 – 2435ExtracellularSequence Analysis
Transmembranei244 – 26421Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Topological domaini265 – 29127CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei292 – 31221Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Topological domaini313 – 3197ExtracellularSequence Analysis
Transmembranei320 – 34021Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Topological domaini341 – 3466CytoplasmicSequence Analysis
Transmembranei347 – 36721Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Topological domaini368 – 41952ExtracellularSequence AnalysisAdd
BLAST
Intramembranei420 – 44021Pore-forming; Name=Segment H5Sequence AnalysisAdd
BLAST
Topological domaini441 – 4466ExtracellularSequence Analysis
Transmembranei447 – 46721Helical; Name=Segment S6Sequence AnalysisAdd
BLAST
Topological domaini468 – 988521CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. integral component of plasma membrane Source: RefGenome
  3. intracellular Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 988988Potassium voltage-gated channel subfamily H member 5PRO_0000054012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis
Cross-linki785 – 785Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Expressioni

Tissue specificityi

Detected in adult testis and in embryonic and adult brain, but not in other tissues. Highly expressed in specific brain areas, such as neocortex, olfactory bulb, primary olfactory cortex and brain stem. In cortex, expression is concentrated in a narrow band toward the middle lamella (layer IV). Moderately expressed in spinal cord, dorsal thalamic nuclei, medial hypothalamus, colliculus, lateral lemniscus, pontine nuclei and Islands of Calleja.

Gene expression databases

GenevestigatoriQ9EPI9.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH1/EAG (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013275.

Structurei

3D structure databases

ProteinModelPortaliQ9EPI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 8673PASAdd
BLAST
Domaini91 – 14353PACPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni704 – 71512Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni909 – 94840CAD (involved in subunit assembly)By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi432 – 4376Selectivity filterBy similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2202.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000230794.
HOVERGENiHBG101348.
InParanoidiQ9EPI9.
KOiK04908.
OMAiGSTQSDP.
OrthoDBiEOG7QG43V.
PhylomeDBiQ9EPI9.
TreeFamiTF313130.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003949. K_chnl_volt-dep_EAG.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01464. EAGCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPI9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGGKRGLVA PQNTFLENIV RRSSESSFLL GNAQIVDWPV VYSNDGFCKL
60 70 80 90 100
SGYHRADVMQ KSSTCSFMYG ELTDKKTIEK VRQTFDNYES NCFEVLLYKK
110 120 130 140 150
NRTPVWFYMQ IAPIRNEHEK VVLFLCTFKD ITLFKQPIED DSTKGWTKFA
160 170 180 190 200
RLTRALTNSR SVLQQLTPMN KTETVHKHSR LAEVLQLGSD ILPQYKQEAP
210 220 230 240 250
KTPPHIILHY CAFKTTWDWV ILILTFYTAI MVPYNVSFKT KQNNIAWLVL
260 270 280 290 300
DSVVDVIFLV DIVLNFHTTF VGPGGEVISD PKLIRMNYLK TWFVIDLLSC
310 320 330 340 350
LPYDIINAFE NVDEGISSLF SSLKVVRLLR LGRVARKLDH YLEYGAAVLV
360 370 380 390 400
LLVCVFGLVA HWLACIWYSI GDYEVIDEVT NTIQIDSWLY QLALSIGTPY
410 420 430 440 450
RYNTSAGIWE GGPSKDSLYV SSLYFTMTSL TTIGFGNIAP TTDVEKMFSV
460 470 480 490 500
AMMMVGSLLY ATIFGNVTTI FQQMYANTNR YHEMLNNVRD FLKLYQVPKG
510 520 530 540 550
LSERVMDYIV STWSMSKGID TEKVLSICPK DMRADICVHL NRKVFNEHPA
560 570 580 590 600
FRLASDGCLR ALAVEFQTIH CAPGDLIYHA GESVDALCFV VSGSLEVIQD
610 620 630 640 650
EEVVAILGKG DVFGDIFWKE TTLAHACANV RALTYCDLHI IKREALLKVL
660 670 680 690 700
DFYTAFANSF SRNLTLTCNL RKRIIFRKIS DVKKEEEERL RQKNEVTLSI
710 720 730 740 750
PVDHPVRKLF QKFKQQKELR NQGSAQSDPE RSQLQVESRP LQNGASITGT
760 770 780 790 800
SVVTVSQITP IQTSLAYVKT SETLKQNNRD AMELKPNGGA EPKCLKVNSP
810 820 830 840 850
IRMKNGNGKG WLRLKNNMGA HEEKKEEWNN VTKAESMGLL SEDPKGSDSE
860 870 880 890 900
NSVTKNPLRK TDSCDSGITK SDLRLDKAGE ARSPLEHSPS QADAKHPFYP
910 920 930 940 950
IPEQALQTTL QEVKHELKED IQLLSCRMTA LEKQVAEILK LLSEKSVPQT
960 970 980
SSPKPQIPLQ VPPQIPCQDI FSVSRPESPE SDKDEINF
Length:988
Mass (Da):111,830
Last modified:March 1, 2001 - v1
Checksum:i888AE96759F64C4A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti397 – 3971G → R in AAF19354. (PubMed:10594062)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF185637 mRNA. Translation: AAF19354.1.
AJ250280 mRNA. Translation: CAC20863.1.
AF073891 mRNA. Translation: AAC61521.1.
RefSeqiNP_598294.1. NM_133610.2.
UniGeneiRn.48753.

Genome annotation databases

EnsembliENSRNOT00000013275; ENSRNOP00000013275; ENSRNOG00000009542.
GeneIDi171146.
KEGGirno:171146.
UCSCiRGD:621417. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF185637 mRNA. Translation: AAF19354.1 .
AJ250280 mRNA. Translation: CAC20863.1 .
AF073891 mRNA. Translation: AAC61521.1 .
RefSeqi NP_598294.1. NM_133610.2.
UniGenei Rn.48753.

3D structure databases

ProteinModelPortali Q9EPI9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000013275.

Chemistry

GuidetoPHARMACOLOGYi 571.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000013275 ; ENSRNOP00000013275 ; ENSRNOG00000009542 .
GeneIDi 171146.
KEGGi rno:171146.
UCSCi RGD:621417. rat.

Organism-specific databases

CTDi 27133.
RGDi 621417. Kcnh5.

Phylogenomic databases

eggNOGi COG2202.
GeneTreei ENSGT00760000118772.
HOGENOMi HOG000230794.
HOVERGENi HBG101348.
InParanoidi Q9EPI9.
KOi K04908.
OMAi GSTQSDP.
OrthoDBi EOG7QG43V.
PhylomeDBi Q9EPI9.
TreeFami TF313130.

Enzyme and pathway databases

Reactomei REACT_199159. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi 621956.
PROi Q9EPI9.

Gene expression databases

Genevestigatori Q9EPI9.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003949. K_chnl_volt-dep_EAG.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view ]
PRINTSi PR01463. EAGCHANLFMLY.
PR01464. EAGCHANNEL.
SMARTi SM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of components of a novel subthreshold-activating K+ channel with a unique pattern of expression in the cerebral cortex."
    Saganich M.J., Vega-Saenz de Miera E., Nadal M.S., Baker H., Coetzee W.A., Rudy B.
    J. Neurosci. 19:10789-10802(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Thalamus.
  2. "Cloning and functional expression of rat eag2, a new member of the ether-a-go-go family of potassium channels and comparison of its distribution with that of eag1."
    Ludwig J., Weseloh R., Karschin C., Liu Q., Netzer R., Engeland B., Stansfeld C., Pongs O.
    Mol. Cell. Neurosci. 16:59-70(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Cerebellum.
  3. "Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia."
    Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E.
    J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 220-360.
    Tissue: Brain.

Entry informationi

Entry nameiKCNH5_RAT
AccessioniPrimary (citable) accession number: Q9EPI9
Secondary accession number(s): O88893, Q9QXT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The channel is down-regulated to 10% residual activity by 400 nano molar cytosolic calcium ions.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3