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Protein

Potassium voltage-gated channel subfamily H member 5

Gene

Kcnh5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi550 – 667cNMPAdd BLAST118

GO - Molecular functioni

  • ion channel binding Source: RGD
  • phosphorelay sensor kinase activity Source: InterPro
  • protein heterodimerization activity Source: RGD
  • voltage-gated potassium channel activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calmodulin-binding, Potassium

Enzyme and pathway databases

ReactomeiR-RNO-1296072. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily H member 5
Alternative name(s):
Ether-a-go-go potassium channel 2
Short name:
rEAG2
Voltage-gated potassium channel subunit Kv10.2
Gene namesi
Name:Kcnh5
Synonyms:Eag2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621417. Kcnh5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 217CytoplasmicSequence analysisAdd BLAST217
Transmembranei218 – 238Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini239 – 243ExtracellularSequence analysis5
Transmembranei244 – 264Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini265 – 291CytoplasmicSequence analysisAdd BLAST27
Transmembranei292 – 312Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini313 – 319ExtracellularSequence analysis7
Transmembranei320 – 340Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini341 – 346CytoplasmicSequence analysis6
Transmembranei347 – 367Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini368 – 419ExtracellularSequence analysisAdd BLAST52
Intramembranei420 – 440Pore-forming; Name=Segment H5Sequence analysisAdd BLAST21
Topological domaini441 – 446ExtracellularSequence analysis6
Transmembranei447 – 467Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini468 – 988CytoplasmicSequence analysisAdd BLAST521

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000540121 – 988Potassium voltage-gated channel subfamily H member 5Add BLAST988

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1
Cross-linki785Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei883PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9EPI9.
PRIDEiQ9EPI9.

PTM databases

iPTMnetiQ9EPI9.
PhosphoSitePlusiQ9EPI9.

Expressioni

Tissue specificityi

Detected in adult testis and in embryonic and adult brain, but not in other tissues. Highly expressed in specific brain areas, such as neocortex, olfactory bulb, primary olfactory cortex and brain stem. In cortex, expression is concentrated in a narrow band toward the middle lamella (layer IV). Moderately expressed in spinal cord, dorsal thalamic nuclei, medial hypothalamus, colliculus, lateral lemniscus, pontine nuclei and Islands of Calleja.

Gene expression databases

BgeeiENSRNOG00000009542.
GenevisibleiQ9EPI9. RN.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH1/EAG (By similarity).By similarity

GO - Molecular functioni

  • ion channel binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013275.

Structurei

3D structure databases

ProteinModelPortaliQ9EPI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 86PASAdd BLAST73
Domaini91 – 143PACPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni704 – 715Calmodulin-bindingSequence analysisAdd BLAST12
Regioni909 – 948CAD (involved in subunit assembly)By similarityAdd BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi432 – 437Selectivity filterBy similarity6

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000230794.
HOVERGENiHBG101348.
InParanoidiQ9EPI9.
KOiK04908.
OMAiNKAEVVH.
OrthoDBiEOG091G0OXR.
PhylomeDBiQ9EPI9.
TreeFamiTF313130.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003949. K_chnl_volt-dep_EAG.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01464. EAGCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EPI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGGKRGLVA PQNTFLENIV RRSSESSFLL GNAQIVDWPV VYSNDGFCKL
60 70 80 90 100
SGYHRADVMQ KSSTCSFMYG ELTDKKTIEK VRQTFDNYES NCFEVLLYKK
110 120 130 140 150
NRTPVWFYMQ IAPIRNEHEK VVLFLCTFKD ITLFKQPIED DSTKGWTKFA
160 170 180 190 200
RLTRALTNSR SVLQQLTPMN KTETVHKHSR LAEVLQLGSD ILPQYKQEAP
210 220 230 240 250
KTPPHIILHY CAFKTTWDWV ILILTFYTAI MVPYNVSFKT KQNNIAWLVL
260 270 280 290 300
DSVVDVIFLV DIVLNFHTTF VGPGGEVISD PKLIRMNYLK TWFVIDLLSC
310 320 330 340 350
LPYDIINAFE NVDEGISSLF SSLKVVRLLR LGRVARKLDH YLEYGAAVLV
360 370 380 390 400
LLVCVFGLVA HWLACIWYSI GDYEVIDEVT NTIQIDSWLY QLALSIGTPY
410 420 430 440 450
RYNTSAGIWE GGPSKDSLYV SSLYFTMTSL TTIGFGNIAP TTDVEKMFSV
460 470 480 490 500
AMMMVGSLLY ATIFGNVTTI FQQMYANTNR YHEMLNNVRD FLKLYQVPKG
510 520 530 540 550
LSERVMDYIV STWSMSKGID TEKVLSICPK DMRADICVHL NRKVFNEHPA
560 570 580 590 600
FRLASDGCLR ALAVEFQTIH CAPGDLIYHA GESVDALCFV VSGSLEVIQD
610 620 630 640 650
EEVVAILGKG DVFGDIFWKE TTLAHACANV RALTYCDLHI IKREALLKVL
660 670 680 690 700
DFYTAFANSF SRNLTLTCNL RKRIIFRKIS DVKKEEEERL RQKNEVTLSI
710 720 730 740 750
PVDHPVRKLF QKFKQQKELR NQGSAQSDPE RSQLQVESRP LQNGASITGT
760 770 780 790 800
SVVTVSQITP IQTSLAYVKT SETLKQNNRD AMELKPNGGA EPKCLKVNSP
810 820 830 840 850
IRMKNGNGKG WLRLKNNMGA HEEKKEEWNN VTKAESMGLL SEDPKGSDSE
860 870 880 890 900
NSVTKNPLRK TDSCDSGITK SDLRLDKAGE ARSPLEHSPS QADAKHPFYP
910 920 930 940 950
IPEQALQTTL QEVKHELKED IQLLSCRMTA LEKQVAEILK LLSEKSVPQT
960 970 980
SSPKPQIPLQ VPPQIPCQDI FSVSRPESPE SDKDEINF
Length:988
Mass (Da):111,830
Last modified:March 1, 2001 - v1
Checksum:i888AE96759F64C4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti397G → R in AAF19354 (PubMed:10594062).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF185637 mRNA. Translation: AAF19354.1.
AJ250280 mRNA. Translation: CAC20863.1.
AF073891 mRNA. Translation: AAC61521.1.
RefSeqiNP_598294.1. NM_133610.2.
UniGeneiRn.48753.

Genome annotation databases

EnsembliENSRNOT00000013275; ENSRNOP00000013275; ENSRNOG00000009542.
GeneIDi171146.
KEGGirno:171146.
UCSCiRGD:621417. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF185637 mRNA. Translation: AAF19354.1.
AJ250280 mRNA. Translation: CAC20863.1.
AF073891 mRNA. Translation: AAC61521.1.
RefSeqiNP_598294.1. NM_133610.2.
UniGeneiRn.48753.

3D structure databases

ProteinModelPortaliQ9EPI9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013275.

PTM databases

iPTMnetiQ9EPI9.
PhosphoSitePlusiQ9EPI9.

Proteomic databases

PaxDbiQ9EPI9.
PRIDEiQ9EPI9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013275; ENSRNOP00000013275; ENSRNOG00000009542.
GeneIDi171146.
KEGGirno:171146.
UCSCiRGD:621417. rat.

Organism-specific databases

CTDi27133.
RGDi621417. Kcnh5.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000230794.
HOVERGENiHBG101348.
InParanoidiQ9EPI9.
KOiK04908.
OMAiNKAEVVH.
OrthoDBiEOG091G0OXR.
PhylomeDBiQ9EPI9.
TreeFamiTF313130.

Enzyme and pathway databases

ReactomeiR-RNO-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiQ9EPI9.

Gene expression databases

BgeeiENSRNOG00000009542.
GenevisibleiQ9EPI9. RN.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003949. K_chnl_volt-dep_EAG.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01464. EAGCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCNH5_RAT
AccessioniPrimary (citable) accession number: Q9EPI9
Secondary accession number(s): O88893, Q9QXT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The channel is down-regulated to 10% residual activity by 400 nano molar cytosolic calcium ions.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.