Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium voltage-gated channel subfamily H member 5

Gene

Kcnh5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Miscellaneous

The channel is down-regulated to 10% residual activity by 400 nano molar cytosolic calcium ions.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi550 – 667cNMPAdd BLAST118

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB-KW
  • ion channel binding Source: RGD
  • phosphorelay sensor kinase activity Source: InterPro
  • protein heterodimerization activity Source: RGD
  • voltage-gated potassium channel activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Ion channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-RNO-1296072 Voltage gated Potassium channels

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily H member 5
Alternative name(s):
Ether-a-go-go potassium channel 2
Short name:
rEAG2
Voltage-gated potassium channel subunit Kv10.2
Gene namesi
Name:Kcnh5
Synonyms:Eag2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621417 Kcnh5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 217CytoplasmicSequence analysisAdd BLAST217
Transmembranei218 – 238Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini239 – 243ExtracellularSequence analysis5
Transmembranei244 – 264Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini265 – 291CytoplasmicSequence analysisAdd BLAST27
Transmembranei292 – 312Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini313 – 319ExtracellularSequence analysis7
Transmembranei320 – 340Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini341 – 346CytoplasmicSequence analysis6
Transmembranei347 – 367Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini368 – 419ExtracellularSequence analysisAdd BLAST52
Intramembranei420 – 440Pore-forming; Name=Segment H5Sequence analysisAdd BLAST21
Topological domaini441 – 446ExtracellularSequence analysis6
Transmembranei447 – 467Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini468 – 988CytoplasmicSequence analysisAdd BLAST521

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000540121 – 988Potassium voltage-gated channel subfamily H member 5Add BLAST988

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi403N-linked (GlcNAc...) asparagineSequence analysis1
Cross-linki785Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei883PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9EPI9
PRIDEiQ9EPI9

PTM databases

iPTMnetiQ9EPI9
PhosphoSitePlusiQ9EPI9

Expressioni

Tissue specificityi

Detected in adult testis and in embryonic and adult brain, but not in other tissues. Highly expressed in specific brain areas, such as neocortex, olfactory bulb, primary olfactory cortex and brain stem. In cortex, expression is concentrated in a narrow band toward the middle lamella (layer IV). Moderately expressed in spinal cord, dorsal thalamic nuclei, medial hypothalamus, colliculus, lateral lemniscus, pontine nuclei and Islands of Calleja.

Gene expression databases

BgeeiENSRNOG00000009542
GenevisibleiQ9EPI9 RN

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH1/EAG (By similarity).By similarity

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB-KW
  • ion channel binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013275

Structurei

3D structure databases

ProteinModelPortaliQ9EPI9
SMRiQ9EPI9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 86PASAdd BLAST73
Domaini91 – 143PACPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni704 – 715Calmodulin-bindingSequence analysisAdd BLAST12
Regioni909 – 948CAD (involved in subunit assembly)By similarityAdd BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi432 – 437Selectivity filterBy similarity6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
GeneTreeiENSGT00900000140833
HOGENOMiHOG000230794
HOVERGENiHBG101348
InParanoidiQ9EPI9
KOiK04908
OMAiDKAGETR
OrthoDBiEOG091G0OXR
PhylomeDBiQ9EPI9
TreeFamiTF313130

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
cd00130 PAS, 1 hit
Gene3Di1.20.120.350, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR030171 EAG2
IPR005821 Ion_trans_dom
IPR003949 K_chnl_volt-dep_EAG
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR001610 PAC
IPR000014 PAS
IPR000700 PAS-assoc_C
IPR035965 PAS-like_dom_sf
IPR014710 RmlC-like_jellyroll
IPR027359 Volt_channel_dom_sf
PANTHERiPTHR10217:SF533 PTHR10217:SF533, 1 hit
PfamiView protein in Pfam
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF13426 PAS_9, 1 hit
PRINTSiPR01463 EAGCHANLFMLY
PR01464 EAGCHANNEL
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SM00086 PAC, 1 hit
SUPFAMiSSF51206 SSF51206, 1 hit
SSF55785 SSF55785, 1 hit
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50042 CNMP_BINDING_3, 1 hit
PS50113 PAC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9EPI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGGKRGLVA PQNTFLENIV RRSSESSFLL GNAQIVDWPV VYSNDGFCKL
60 70 80 90 100
SGYHRADVMQ KSSTCSFMYG ELTDKKTIEK VRQTFDNYES NCFEVLLYKK
110 120 130 140 150
NRTPVWFYMQ IAPIRNEHEK VVLFLCTFKD ITLFKQPIED DSTKGWTKFA
160 170 180 190 200
RLTRALTNSR SVLQQLTPMN KTETVHKHSR LAEVLQLGSD ILPQYKQEAP
210 220 230 240 250
KTPPHIILHY CAFKTTWDWV ILILTFYTAI MVPYNVSFKT KQNNIAWLVL
260 270 280 290 300
DSVVDVIFLV DIVLNFHTTF VGPGGEVISD PKLIRMNYLK TWFVIDLLSC
310 320 330 340 350
LPYDIINAFE NVDEGISSLF SSLKVVRLLR LGRVARKLDH YLEYGAAVLV
360 370 380 390 400
LLVCVFGLVA HWLACIWYSI GDYEVIDEVT NTIQIDSWLY QLALSIGTPY
410 420 430 440 450
RYNTSAGIWE GGPSKDSLYV SSLYFTMTSL TTIGFGNIAP TTDVEKMFSV
460 470 480 490 500
AMMMVGSLLY ATIFGNVTTI FQQMYANTNR YHEMLNNVRD FLKLYQVPKG
510 520 530 540 550
LSERVMDYIV STWSMSKGID TEKVLSICPK DMRADICVHL NRKVFNEHPA
560 570 580 590 600
FRLASDGCLR ALAVEFQTIH CAPGDLIYHA GESVDALCFV VSGSLEVIQD
610 620 630 640 650
EEVVAILGKG DVFGDIFWKE TTLAHACANV RALTYCDLHI IKREALLKVL
660 670 680 690 700
DFYTAFANSF SRNLTLTCNL RKRIIFRKIS DVKKEEEERL RQKNEVTLSI
710 720 730 740 750
PVDHPVRKLF QKFKQQKELR NQGSAQSDPE RSQLQVESRP LQNGASITGT
760 770 780 790 800
SVVTVSQITP IQTSLAYVKT SETLKQNNRD AMELKPNGGA EPKCLKVNSP
810 820 830 840 850
IRMKNGNGKG WLRLKNNMGA HEEKKEEWNN VTKAESMGLL SEDPKGSDSE
860 870 880 890 900
NSVTKNPLRK TDSCDSGITK SDLRLDKAGE ARSPLEHSPS QADAKHPFYP
910 920 930 940 950
IPEQALQTTL QEVKHELKED IQLLSCRMTA LEKQVAEILK LLSEKSVPQT
960 970 980
SSPKPQIPLQ VPPQIPCQDI FSVSRPESPE SDKDEINF
Length:988
Mass (Da):111,830
Last modified:March 1, 2001 - v1
Checksum:i888AE96759F64C4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti397G → R in AAF19354 (PubMed:10594062).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF185637 mRNA Translation: AAF19354.1
AJ250280 mRNA Translation: CAC20863.1
AF073891 mRNA Translation: AAC61521.1
RefSeqiNP_598294.1, NM_133610.2
UniGeneiRn.48753

Genome annotation databases

EnsembliENSRNOT00000013275; ENSRNOP00000013275; ENSRNOG00000009542
GeneIDi171146
KEGGirno:171146
UCSCiRGD:621417 rat

Similar proteinsi

Entry informationi

Entry nameiKCNH5_RAT
AccessioniPrimary (citable) accession number: Q9EPI9
Secondary accession number(s): O88893, Q9QXT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health